Physical Sciences and Mathematics Commons^™

Comparison Of The In Vitro Fermentation Characteristic Of Fractionated Alfalfa And Sainfoin, L R. Mcmahon, M Goto, T A. Mcallister, G A. Jones, K J. Cheng

IGC Proceedings (1997-2023)

Alfalfa and sainfoin leaves were lyophilized and ground (A, S, respectively) or were fractionated into soluble (A_SOL, S_SOL) and insoluble (A_INS, S_INS)components and lyophilized and incubated in vitro with diluted ruminal fluid for 24h. Whole leaf and soluble fractions were also incubated with polyethylene glycol 8000 (PEG). Gas production (GP), ammonia concentration [NH3] and volatile fatty acid (VFA) production were lower (P< 0.05) from S and SSOL than from A and ASOL Insoluble fractions differed only in [NH3] (SINS < AINS, P < 0.05). Inclusion of PEG increased (P < 0.05) GP, [NH3] and VFA production from whole leaf and soluble fractions of sainfoin, but not alfalfa. Inactivating the condensed tannins in sainfoin with PEG overcame differences in degradability between these two forages.

Identification Of Alfalfa Chromosomes Using Giemas Banding And Image Analysis Techniques, G R. Bauchan, M A. Hossain

IGC Proceedings (1997-2023)

Chromosomes of two diploid (2n=2x=16) subspecies of Medicago sativa, ssp. caerulea and ssp. falcata, their hybrid and tetraploid (2n=4x=32) cultivated alfalfa (M. sativa, ssp. sativa) were studied. Feulgen’s staining, C- and N-banding techniques and an image analysis system were used. The chromosomes of ssp. falcata have only centromeric bands, however, a multitude of bands were observed in both the C- and N-banding pattern of ssp. caerulea and ssp. sativa enabling the precise identification of each of the eight sets of chromosomes and development of a karyotype. The differences in banding patterns between the diploid subspecies makes it possible …

Aluminum Tolerance Qtl In Diploid Alfalfa, M K. Sledge, J H. Bouton, J Tamulonis, W A. Parrot, G Kochert

IGC Proceedings (1997-2023)

Aluminum (Al) toxicity associated with acid soils greatly inhibits alfalfa (Medicago sativa L.) productivity throughout much of the world’s major grassland areas. In this paper, we report the identification of quantitative trait loci (QTL) controlling aluminum tolerance in diploid alfalfa (Medicago sativa L). An in vitro callus growth bioassay was used to select aluminum tolerant and aluminum sensitive parents, and to screen an F2 population for aluminum tolerance. Fifty-eight cDNA probes were mapped to nine linkage groups, and the F2 genotypic classes were contrasted with means from the callus growth bioassay using ANOVA. We also used Mapmaker-QTL to …

Comparison Of Composition And Degradation Characteristics Of Early Bloom Alfalfa With Fenugreek (Trigonella Foenum-Graecum) Forages Harvested At Different Stages Of Maturity, Z Mir, P S. Mir, S N. Acharya, L A. Goonewardene

IGC Proceedings (1997-2023)

The objective of this study was to compare the composition and degradation characteristics of early bloom alfalfa and fenugreek harvested after 9, 15 and 17 wk of growth. Crude protein (CP) content of fenugreek cut at 9, 15 and 17 wk was lower (P<0.05) than that of alfalfa. Neutral detergent fibre (NDF) and lignin content of fenugreek harvested after 15 and 17 wk were higher (P<0.05) than that of alfalfa. Ash content was lower (P<0.05) in fenugreek than for alfalfa. In vitro dry matter disappearance (IVDMD) of fenugreek cut at 15 and 17 wk was similar to that of alfalfa. Total in vitro gas production of fenugreek cut at the three stages of growth was not significantly different from those observed for alfalfa. Results indicate that chemical composition and IVDMD of fenugreek at all stages of …

Quality Evaluation Of Feed Resources At The Newly Reclaimed Area In Egypt, M K. Hathout, F Z. Swidan, M F. El-Sayes, H A. Eid

IGC Proceedings (1997-2023)

The objective of this study was to examine the productivity of Egyptian clover (Trifolium alexandrinum L.) interseeded with ryegrass (Lolium multiflorum) and alfalfa (Medicaga sativa L.). These species were planted in newly reclaimed land in Ismailia, Egypt under two fertilization systems; organic (OF) or chemical (CF) during winter season of (1994-1995) using a split plot design. Feeding quality of forages was evaluated by conducting digestibility trials with rams and feeding trials with growing lambs where forage was offered ad. libitum with 1% of LBW concentrate. Productivity data indicated that organic fertilization yielded significantly more DM. Chemical …

Ecosystem Services And Life Cycle Assessment Of Perennial And Annual Cropping Systems, M. Berti, A. Cecchin

IGC Proceedings (1997-2023)

Corn cultivation has negative impacts to the environment, such as nitrate leaching, soil erosion, and nitrous oxide emissions. Perennial crops such as alfalfa (Medicago sativa L.) reduce annual disturbance of soil, which affects many biogeochemical cycles that are key to provide resilience and stability to cropping systems. Four three-year crop sequences were evaluated. 1) Corn-soybean-corn (CSC); 2) alfalfa-alfalfa-alfalfa (AAA); 3) Corn-spring planted alfalfa-alfalfa (CAA); and 4) Corn-intercropped/alfalfa-alfalfa-alfalfa (CAIAA). Modeling of C and N cycles were conducted with the Denitrification-Decomposition (DNDC) model and global warming potential (GWP) was estimated using life cycle assessment (LCA) methodology. The soil organic carbon (SOC) balance …

Drought Tolerance Dissection And Molecular Breeding In Alfalfa, Luo Dong, Qiang Zhou, Xueming Dong, Wenxian Liu, Zhipeng Liu

IGC Proceedings (1997-2023)

Drought stress is one of the leading impediments that limit the productivity of global alfalfa (Medicago sativa). The underlying molecular and genetic mechanisms for drought tolerance in alfalfa remain largely unclear. In order to fully reveal the transcriptional changes of alfalfa in response to abiotic stress, the alfalfa transcriptome database under mannitol (simulated drought stress), NaCl (simulated salt stress), or exogenous ABA application was built via various RNA-seq technologies. Through further screening of the transcriptome database, a number of genes significantly induced by drought stress, such as the Nuclear Transport Factor 2-like (MsNTF2L), Drought-Induced Unknown Protein 1 (MsDIUP1), …

Winter Performance Of North American Bos Grunniens Offered Hay Harvested From Different Forage Species, Jeff Lehmkuhler, G. Dike, M. Mccarty

IGC Proceedings (1997-2023)

Bos grunniens or the domesticated yak was brought to North America in the late 1800’s and early 1900’s. In Asia, yaks have been observed to lose 25% or more of their body weight over the winter in the high mountain altitudes. Little research has been conducted with the domesticated yak and their nutritional needs. Maintaining positive weight change during the winter is expected to improve the profitability and viability of yak production systems in North America. An on-farm study was conducted near Wellington, KY, USA to investigate the winter performance of yaks and hay type. Four outdoor grass lots approximate …

Developing A Simple Bioassay For Detection Of Alfalfa Autotoxicity In Field Soils, P. Baisley, K. Cassida, S. Gruss, E. Hill

IGC Proceedings (1997-2023)

Alfalfa autotoxicity causes yield reductions in alfalfa production by inhibiting plant establishment and decreasing plant productivity. Accurate predictions regarding autotoxic potential of the soil in a given field at a given time are an essential tool for alfalfa growers to make appropriate planting decisions. To address this issue, we are developing a soil bioassay that can be conducted as a mail-in soil test for alfalfa growers through plant diagnostic service laboratories. We hypothesize that we will detect differences in seed germination, emergence, root length, and root morphology between control and autotoxic soils. A preliminary trial testing alfalfa field soils and …

Grazing Management For Alfalfa Persistence And Productivity In Warm-Climate Regions, D. Basigalup

IGC Proceedings (1997-2023)

Alfalfa is the most important cultivated forage crop in Argentina, where about 3 million hectares are grown. Even though the use of direct grazing is coming down during the last 15 years, it is still important for both beef and dairy production. Alfalfa grazing can play an important role in reducing operative costs and decreasing quality loss due to forage conservation (hay or silage). In this paper, the main concepts necessary to implement adequate and practical rotational grazing systems are briefly discussed. Particular attention is given to issues such as grazing frequency, grazing period, pasture use efficiency, and alfalfa quality …

Assessing The Nutrient Status Of Alfalfa Stands In Kentucky, William R. Fleming, Christopher D. Teutsch, Edwin L. Ritchey, John H. Grove

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is a perennial forage legume and is sometimes referred to as the “Queen of Forages” due to its high nutritional value. Alfalfa is commonly produced as hay for use in the dairy and equine industries. Alfalfa yield increased exponentially from the 1950s to the1980s due to new breeding and management innovations. However, during the 1980s yields plateaued at around 7.5 Mg/ha and remain there today. The overarching goal of this study was to determine the role of soil fertility in the observed yield plateau. Fifty alfalfa stands in Kentucky were sampled to gather information about …

Prospects For Improving Alfalfa Yield Using Genomic- And Phenomic-Based Breeding, M. W. Francis, D. Pap, A. Krill-Brown, E. C. Brummer

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is a perennial outcrossing legume that is cultivated as an important forage crop in many parts of the world. Yield is the most important trait for profitable alfalfa production, yet over the last 30 years yield improvement in California has stagnated. Current breeding methods focus on recurrent phenotypic selection; however, alternatives incorporating genomic- and phenomic-based information may enhance genetic gain and help to address the lack of yield improvement. Here we attempt to increase the yield potential of alfalfa using genomic selection (GS) in combination with high throughput phenotyping (HTP). A total of 193 families …

Effects Of Fall Dormancy, Cutting Frequency, And K Application Rate On Dry Matter Yield And Nutritive Value Of Alfalfa, Ji Yung Kim, J. S. Choi, M. H. Jo, K. I. Sung

IGC Proceedings (1997-2023)

[Introduction] This study was conducted to determine the effect of the cutting frequency and K application rate on the dry matter yield (DMY) and nutritive value of new alfalfa varieties in Korea.

[Material and Methods] The sowing date and rate were September 21, 2019, and 20 kg/ha, respectively. The treatment was by the split-split plot design, and the main plot was the Fall dormancy (FD) of 3 (Rugged Rancher), 4 (SW4113), and 9 (SW9720), the sub-plot was excess 40 day (LCF), from 31 to 40 days (MCF), and under 30 day (HCF) times of cutting frequency …

Irrigation Effects On Forage Dry Matter Yield And Nutritive Value Of Alfalfa, Rudra Baral, Doohong Min

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is among the top four field crops in United States (U.S.) in terms of acreage harvested and economic value. Several studies have documented that alfalfa requires high amount of water compared to other major field crops. However, more than 65% alfalfa in the U.S. is grown under rainfed condition where severe to moderate drought condition has been pronounced during alfalfa growing season. The magnitude of yield loss due to water-limited conditions is still unknown. In this context, the objective of our study is to evaluate the forage dry matter yield and forage nutritive value of …

Farmer Experiences With Alfalfa In The Southern U.S.A., M. K. Mullenix, L. S. Silva, C. G. Prevatt, J. J. Tucker

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is a forage crop of primary importance in the United States (USA); however, adoption and use in the southern USA region has been limited. The combination of adapted alfalfa cultivars and management strategies which demonstrate extended forage growing season length, improved forage nutritive value, and reduced N fertilizer inputs have renewed interest in forage-livestock farmers to consider planting alfalfa. A series of Extension-based educational strategies have been used in the southern USA to 1) create awareness on the use of alfalfa, 2) educate farmers regarding establishment and management practices, and 3) showcase opportunities and challenges …

Effects Of Insecticide And Tolerant Alfalfa Cultivars On Potato Leafhopper (Empoasca Fabae) Populations And Forage Yields In Quebec (Canada), Philippe Seguin, X. Shi, J. Saguez, Huguette Martel, A. Claessens

IGC Proceedings (1997-2023)

The potato leafhopper [PLH, Empoasca fabae (Harris)], which affects several crops including alfalfa (Medicago sativa L.), is a recurrent problem in several regions of Quebec. The objective was to evaluate alfalfa management tools in order to reduce yield losses caused by this pest. An experiment was conducted at two sites in Quebec over three field seasons to evaluate the impact of insecticide applications and the use of PLHtolerant cultivars on forage yield and PLH populations. Foliar insecticide applications in the seeding year reduced PLH populations but generally failed to impact alfalfa yields compared to untreated alfalfa. However, in one …

A New Perennial Legume To Combat Dryland Salinity In South-Western Australia, L. W. Bell, M. A. Ewing, M. Ryan, S. J. Bennett, G. A. Moore

IGC Proceedings (1997-2023)

Dryland salinity has devastated large tracts of productive land in Australia. This has resulted from the clearing of native perennial vegetation and its replacement with annual crops and pastures. As annual plants are shallow rooted and only use water during their winter-spring growing season, unutilised rainwater leaks into groundwater tables which rise and bring stored salt to the soil surface. The adoption of deep rooted perennial pasture plants that increase the water use can help to manage dryland salinity whilst maintaining productivity. However, new plants are needed as few perennial pasture options currently exist. Preliminary research into the potential of …

Soil Carbon Sequestration Under Three Years Of No-Till Forage Cropping Systems, D. H. Min, J. D. Deyoung, Richard Leep

IGC Proceedings (1997-2023)

The effects of reduced tillage on soil organic carbon (SOC) are generally well established (Chan et al., 2002; Cabardella & Elliott, 1992). The effects of different crops are also somewhat understood (Drinkwater et al., 1998). However, many of these studies are done in the laboratory to study the effects of crop residues on SOC. Many forage-based systems have very little crop residue returned to the field. What residue does return is often in the form of manure or compost, which is usually broken down much more rapidly than most crop residues. The objective of this study is to …

Antioxidative Activities Of Alfalfa And Timothy Varieties, J. K. Lee, H. S. Park, J. G. Kim, B. H. Paek, J. H. Fike

IGC Proceedings (1997-2023)

The term "functional foods" is often used as a generic description for the beneficial effects of ingested foods that go beyond their traditional nutritive value (Bauman et al., 2001). Milk and dairy products are important dietary sources of nutrients, providing energy, high quality protein, and a variety of vitamins and minerals. Recent research has focused on altering the fat and protein content of milk and other dairy products in order to improve their nutrient content to more aptly reflect current dietary recommendations and trends. As a result, additional focus is being given to designing foods that have beneficial effects …

Increased Cuticular Wax Accumulation And Enhanced Drought Tolerance In Transgenic Alfalfa By Overexpression Of A Transcription Factor Gene, Z. -Y. Wang, J. -Y. Zhang, C. Broeckling, E. Blancaflor, M. Sledge, L. Summer

IGC Proceedings (1997-2023)

Plant cuticular waxes play an important role in protecting aerial organs from damage caused by multiple environmental stresses such as drought, cold, UV radiation, pathogen infection and insect attack. The identification of leaf wax genes involved in stress tolerance is expected to have great potential for crop improvement. Cuticular waxes are complex mixtures of very long chain fatty acids, alkanes, primary and/or secondary alcohols, aldehydes, ketones, esters, triterpenes, sterols and flavonoids. Mutant analysis in Arabidopsis has contributed to the identification of the components and genes involved in wax deposition. However, no information is available on the effects of overexpression of …

Development Of Alfalfa (Medicago Sativa L.) Transgenic Plants Expressing A Bacillus Thuringiensis Endotoxin And Their Evaluation Against Alfalfa Caterpillar (Colias Lesbia), F. Ardila, M. C. Gómez, M. J. Diéguez, E. M. Pagano, M. Turica, R. Lecuona, V. Arolfo, D. Basigalup, C. Vázquez Rovere, E. Hopp, P. Franzone, R. D. Rios

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is the most important forage crop in Argentina, with ca. 6 million cultivated hectares. The production of this crop is limited by the alfalfa caterpillar (Colias lesbia) which causes a loss equivalent to at least 10% of the biomass per year. No natural tolerance against this lepidoptera was found in alfalfa germplasm, hampering the development of tolerant cultivars by conventional breeding. This pest is usually controlled by using chemical insecticides but this has adverse effects on beneficial insects and the environment. Alternatively, low doses of commercial Bt insecticides (40 to 70 g/ha) also …

A Medicago Truncatula Population Segregating For Aluminum Tolerance, M. Sledge, B. Narasimhamoorthy, G. Jiang

IGC Proceedings (1997-2023)

Aluminium (Al) toxicity, manifested in inhibition of root elongation and reduced plant growth, is a major cause of poor crop yields on acid soils, which comprise up to 40% of the world’s arable land. Al toxicity associated with acid soils has been a major obstacle in alfalfa (Medicago sativa) production in the USA, as well as in tropical areas of the world. Recent molecular marker mapping studies indicate that the genomes of M. truncatula and M. sativa are highly similar (Choi et al., 2004). Thus, M. truncatula could be used as a source of genes that could …

A New Perennial Legume To Combat Dryland Salinity In South-Western Australia, L. W. Bell, M. A. Ewing, M. Ryan, S. J. Bennett, G. A. Moore

IGC Proceedings (1997-2023)

Dryland salinity has devastated large tracts of productive land in Australia. This has resulted from the clearing of native perennial vegetation and its replacement with annual crops and pastures. As annual plants are shallow rooted and only use water during their winter-spring growing season, unutilised rainwater leaks into groundwater tables which rise and bring stored salt to the soil surface. The adoption of deep rooted perennial pasture plants that increase the water use can help to manage dryland salinity whilst maintaining productivity. However, new plants are needed as few perennial pasture options currently exist. Preliminary research into the potential of …

Translational Genomics For Alfalfa Varietal Improvement, G. D. May

IGC Proceedings (1997-2023)

Key points

1. Medicago truncatula is a model legume with available mapping, genome, and RNA, protein and metabolite profiling databases and genetically diverse populations.
2. Genomics resources developed for M. truncatula have application in the study and improvement of alfalfa making it an excellent model for this forage legume.

Visual Modelling Of Alfalfa Growth And Persistence Under Grazing, S. R. Smith Jr., L. Muendermann, A. Singh

IGC Proceedings (1997-2023)

A ‘virtual’ alfalfa plant model was developed at the University of Manitoba in Canada as part of a comprehensive grazing research project. This model shows an alfalfa plant ‘growing’ on a computer screen and the plant’s response to grazing (similar to time-lapse photography). The original model was constructed by Singh (2005) to show the research potential of visually modelling alfalfa plant growth. The ability to visually ‘grow’ a plant on a computer screen also offers tremendous opportunities for teaching and extension. Detailed morphological measurements were used in the construction of Singh’s model, based on single plants subjected to the following …

Lucerne Crown And Taproot Biomass Affected Early-Spring Canopy Expansion, E. I. Teixeira, D. J. Moot, A. L. Fletcher

IGC Proceedings (1997-2023)

Leaf area index (LAI) quantifies canopy expansion in crops and is used in lucerne (Medicago sativa L.) simulation models to predict daily PAR interception (PAR i). This then drives yield through radiation use efficiency (RUE) (Gosse et al., 1984). In perennial crops, like lucerne, the level of biomass stored in crown and taproot may affect canopy expansion in subsequent regrowth cycles (Avice et al., 1997). In temperate regions the impact of this is likely to be greatest in early-spring, when low temperatures delay development. The objective of the current research was to identify whether contrasting levels of winter biomass in …

Autumn Root Reserves Of Lucerne Affected Shoot Yields During The Following Spring, D. J. Moot, E. I. Teixeira

IGC Proceedings (1997-2023)

Frequent grazing affects shoot yield of lucerne (Medicago sativa L.) by limiting radiation interception (Teixeira et al., 2005b) and the accumulation of endogenous reserves (C and N) in perennial storage organs like crowns and taproots (Teixeira et al., 2005a). In temperate regions, the impact of low level of perennial reserves is particularly evident during early-spring, when lucerne regrowth resumes after an overwintering period. The analysis of lucerne yield can be fragmented into its yield components of plant population, shoots per plant and yield per shoot (Volenec et al., 1987). The objective of this research was to quantify …

Supplementation Under Intensive Grazing, Silage- Or Grain-Based Diets For Beef Production On Steer Performance And Meat Fatty Acid Composition, J. Martínez Ferrer, E. Ustarroz, C. G. Ferrayoli, A. R. Castillo, D. Alomar

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) is the main cultivated pasture in Argentina. In beef production enhanced productivity and profit depend on high stocking rates and pasture utilisation, with grain supplementation necessary to maintain high individual live weight gains (LWG) and to increase production per ha (Ustarroz, 1999). Substitution of grazed grass by concentrate can affect meat fatty acid (FA) composition (French et al., 2000). The objective of this study was to evaluate the effects of intensifying an alfalfa-based grazing system and two confinement dietary regimens for beef steer finishing on animal performance and meat FA composition.

Effect Of Stage Of Maturity On The Nutrient Content Of Alfalfa, Y. Tyrolova, A. Vyborna

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa), one of the major agricultural crops in the Czech Republic, is grown on 15.8% of the arable land. Alfalfa silage forms a substantial part of diets for farm animals. It is very important to choose a suitable term of harvesting for ensilage from the view of optimal nutrient content. The stage of maturity at cutting has large effects on each component, except crude protein (Yu et al., 2004). Alfalfa can be difficult to ensile due to a high buffering capacity and a low WSC content.

The Effects Of Alfalfa Silage Harvesting Systems On Dry Matter Intake Of Friesland Dairy Ewes In Late Pregnancy, H. F. Elizalde

IGC Proceedings (1997-2023)

With the recent introduction of alfalfa in Chilean Patagonia (Aisén), its utilisation as silage has to be reviewed relative to animal performance. The effect of silage chop length on the voluntary intake has been evaluated in different species, with sheep being more sensitive to chop length than cattle (Dulphy et al., 1984). The objective of this experiment was to evaluate the effects of different alfalfa silage chop lengths on dry matter (DM) intake and eating behaviour of Friesland dairy ewes in late pregnancy.