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Full-Text Articles in Physical Sciences and Mathematics

Evaluation Of Blight Resistance In Chestnut F2 Half-Sibling And Full-Sibling Families Via Small Stem Assay, Kevin Gentner Aug 2018

Evaluation Of Blight Resistance In Chestnut F2 Half-Sibling And Full-Sibling Families Via Small Stem Assay, Kevin Gentner

Honors Theses

In 1904, Cryphonectria parasitica, the causal agent for chestnut blight, was imported into North America on chestnut nursery stock from China. Fifty-five years later, nearly all full-grown wild American chestnut trees (Castanea dentata) were dead. A century of work has been put into restoring the American chestnut to its rightful place among the forest canopy. Since the 1980s, The American Chestnut Foundation has pursued backcross breeding to introgress blight resistance into C. dentata from the resistant Chinese species (C. mollissima), and has used progeny testing to make predictions about parental resistance in B3F2 chestnuts. We performed a small stem assay …


Conservation And Collection Of Castanea Dentata Germplasm In The South, Trent Deason May 2018

Conservation And Collection Of Castanea Dentata Germplasm In The South, Trent Deason

Honors Theses

The American chestnut, Castanea dentata, has been devastated by the exotic invasive pathogens Cryphonectria parasitica and Phytophthora cinnamomi to which it has no resistance. The American Chestnut Foundation (TACF) has developed an interspecific backcross breeding program to introgress disease resistance from Asian chestnut species, primarily Castanea mollissima, into C. dentata hybrid populations. The genetic base of this program can be expanded by utilizing vegetative propagation through grafting in order to collect and conserve American chestnut individuals not amenable to traditional breeding. As the majority of the surviving American chestnuts are confined to the understory, they are shaded out by the …


Implementing Early Screening Methods To Detect Resistance To Phytophthora Cinnamomi In Backcross Chinese-American Chestnut Hybrids, Kirsten Hein May 2018

Implementing Early Screening Methods To Detect Resistance To Phytophthora Cinnamomi In Backcross Chinese-American Chestnut Hybrids, Kirsten Hein

Honors Theses

Phytophthora root rot (PRR), caused by the oomycete Phytophthora cinnamomi Rands., is one of the two greatest obstacles to survival of American chestnut (Castanea dentata Borkh.). The other is chestnut blight, caused by the ascomycete Cryphonectria parasitica (Murr.) Barr. Developing early and reliable PRR screening methods can facilitate the efficient introgression of PRR resistance from Chinese chestnut (C. mollissima Blume.) into the populations of potentially chestnut blight resistant trees currently under development by The American Chestnut Foundation (TACF). This study tests the efficacy of a method for early identification of PRR-resistant hybrid chestnuts in a greenhouse/nursery setting. The chestnut blight …


Measuring Phytophthora Resistance Phenotypes In Segregating Testcross Families Of Hybrid American Chestnut Trees, Anna C. Robinson May 2016

Measuring Phytophthora Resistance Phenotypes In Segregating Testcross Families Of Hybrid American Chestnut Trees, Anna C. Robinson

Honors Theses

Phytophthora root rot (PRR), caused by the oomycete Phytophthora cinnamomi Rands, is a formidable obstacle to the restoration of Castanea dentata Marsh. commonly known as the American chestnut. Genetic resistance to PRR has been observed in Asian species of chestnut including C. mollissima Blume, and in interspecific hybrids between C. mollissima and C. dentata. We hypothesized that root rot resistance alleles would segregate in a 1:1 ratio within progeny of crosses between PRR resistant F1 hybrids and PRR-susceptible American chestnut trees (first-backcrosses), and that PRR resistance could be successfully passed down to all families of first-backcross hybrids. To test these …


The Effect Of Phosphite On Mycorrhiza Formation In American Chestnut (Castanea Dentata), Matthew T. Perkins May 2012

The Effect Of Phosphite On Mycorrhiza Formation In American Chestnut (Castanea Dentata), Matthew T. Perkins

Honors Theses

One of the primary hindrances to Castanea dentata restoration in the Southeast is the root rot disease caused by the fungus-like microorganism Phytophthora cinnamomi. Root rot can be combated by the application of mono- and di-potassium salts of phosphorous acid, which are marketed as phosphite fungicides. Despite its value in preventing infection by P. cinnamomi it is also thought that phosphite may impede root colonization by beneficial, ectomycorrhizal fungi. I hypothesized that plants given a routine application of phosphite will display fewer mycorrhizas in the root tips than those plants that were not treated with potassium phosphite. Therefore I attempted …