Physical Sciences and Mathematics Commons^™

Examining Artifacts Of The Watershed Segmentation, Emily Jo Armitage

Electronic Theses and Dissertations

The watershed segmentation is an algorithm used to systematically track cell intercalary behaviors during germ band extension of the Drosophila embryo. Neighboring cells share a contracting vertical interface, called a T1, which continues contracting to a single point, a T2, and extending in the horizontal direction to create what is called a T3 interface (Fig. 1). Additionally, higher order vertices called rosettes occur when five or more cells meet at a common vertex. Simulated T2 events demonstrate that cell angle and not noise level in the image contributes to the incorrect detection of artifactual T1s in more acute angled cells …

Rock Glacier Development In The San Juan Mountains, Brandon K. Bailey

Electronic Theses and Dissertations

Rock glaciers are common landform features found in deglaciated alpine areas. They are commonly used in the study of climatic changes throughout the Holocene and the reconstruction of neoglacial chronologies. For this research, Schmidt hammer rebound values, weathering rind thicknesses, and the length of lichen thalli diameters found on rock glacier surfaces are used to investigate their effectiveness as field-based relative age determination techniques. Additionally, the ability to identify periods of neoglacial activity using these methods is assessed in two neighboring cirque basins in the San Juan Mountains of Colorado. 41 field sites across three rock glaciers are established with …

Controlling Transition Metal-Catalyzed Alkyne Annulations Utilizing Polarized Ynol Ethers, Brandon L. Coles-Taylor

Electronic Theses and Dissertations

Transition metal-catalyzed alkyne annulations have developed into incredibly powerful synthetic tools over there the past quarter century. These reactions provide rapid access to important organic scaffolds such as indole, quinoline, isoquinoline, indene, and isocoumarin scaffolds. Transition metal mediated alkyne annulations have proven invaluable in synthetic fields, such as natural product total synthesis, by offering efficient pathways to otherwise synthetically difficult to access substrates.

Foundational works performed by chemist such as Larock, Ackermann, Satoh, and Miura have been established through relying upon the usage of symmetrical alkynes. When unsymmetrical alkynes are used in annulation processes mixtures of regioisomers are often isolated. …

Development Towards An Ethylene Sensing Artificial Metalloenzyme, Mitchell Lee Ellinwood

Electronic Theses and Dissertations

Ethylene (ethene) is the essential ripening hormone for climacteric plants, and consequently its detection and quantification is of considerable interest to the horticulture industry. Its detection however is a non-trivial pursuit, traditionally requiring expensive and inaccessible instrumentation that fail to provide in vivo information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium …

A Spectropolarimetric Study Of Southern Wr + O Binaries, Andrew G. Fullard

Electronic Theses and Dissertations

The classical Wolf-Rayet (WR) state is the evolved stage of a massive star, post main-sequence. They are characterized by their strong emission line spectra and stellar winds that are often more than 10 times denser than that of their progenitor O-type stars, which have mass loss rates of 10^-6 M_Θyr^-1. The evolution of WR stars and their connection to specific types of supernovae (SNe) is an open question. Current theory suggests that rapidly rotating massive stars may be the progenitors of SNe that produce long-duration gamma-ray bursts. The interaction between WR stars and their companion …

Sensitivity Of Radical Sam Enzyme Mftc To Molecular Oxygen, Vishnu Govindarajan

Electronic Theses and Dissertations

MftC is a radical S-adenosyl L-methionine (SAM) enzyme that catalyzes the first step in the production of mycofactocin. It is a ribosomally synthesized post-translationally modified (RiPP) redox cofactor shown to be essential for the survival of bacteria in the Mycobacterium genus in the presence of cholesterol as a carbon source and for the sequestration of ethanol. MftC catalyzes the C – terminal decarboxylation of tyrosine and the subsequent cross-linking of tyrosine to the penultimate valine on the precursor peptide MftA. The product formed out of the reaction is processed into mycofactocin in downstream processes. The bacteria in the Mycobacterium tuberculosis …

Spatial And Temporal Controls On Streamflow Variability In The San Juan Mountains, Colorado, Christopher Lewis Hancock

Electronic Theses and Dissertations

This project characterizes and examines changes to the annual hydroclimatic cycle throughout alpine regions of Colorado with a focus on trends in snowpack and snowmelt hydrology. Datasets analyzed for this research include 79 SNOTEL sites throughout Colorado (24 in the San Juan Mountains) which provide climate metrics for Water Years 1988-2018. Impacts on streamflow are evaluated in the San Juan Region through a network of 11 USGS stream gauges. Correlation matrices and linear regression methods examine the relative controls on the magnitude and timing of discharge, and trend detection using the regional Kendall test quantifies the rate of change within …

Climate Change And Seed Security Among Smallholder Farmers In Northern Ghana, Michael Biwalib Madin

Electronic Theses and Dissertations

Smallholder agriculture is highly susceptible to climate variability and change. According to recent projections by the Intergovernmental Panel on Climate Change, this sensitivity would likely increase in the coming decades, with more erratic rainfall, prolonged dry periods, shorter growing seasons, and seed germination failures. In the African context, the mechanisms through which these ecological stressors would affect seed security are poorly understood. Drawing upon a case study of semi-arid Ghana, this study examines climate change impacts on seed security among smallholder farmers. It adopts a mixed-methods approach with intensive fieldwork in two farming communities. Conceptually, the study uses a political …

Quantification Of Dynamic Epithelial Sheet Architecture In Botryllus Schlosseri Using 2-D & 3-D Image Analysis, Roopa Madhu

Electronic Theses and Dissertations

Epithelial tubules form critical structures in various body tissues; how- ever, since they are difficult to access experimentally, their architecture and dynamics are not well understood. Here we examine the dynamic remodeling of epithelial tubes in vivo using a novel and uniquely accessible model system: the extracorporeal vasculature of Botryllus schlosseri (sea squirt). In Botryllus, massive retraction of blood vessels can be triggered without loss of barrier function, through (i) disrupting collagen crosslinking in the basement membrane using β-aminopropionitrile (BAPN); or (ii) disrupting the integrin pathway through inhibition of focal adhesion kinase (FAK). We performed stereographic projections of 3-dimensional …

Instrument And Application Development In Saturation Recovery And Rapid Scan Electron Paramagnetic Resonance, Joseph E. Mcpeak

Electronic Theses and Dissertations

Enhanced signal sensitivity by the use of Rapid Scan (RS) electron paramagnetic resonance (EPR), a technique that allows for much faster magnetic field scans than traditional field-swept techniques, has facilitated improved data acquisition for many types of samples. For example, irradiated fingernails for radiation dosimetry have been studied using RS-EPR, which resulted in substantial decreases in detection limits. Samarium-mediated reduction mechanisms in organic synthesis have been investigated by RS-EPR providing evidence for a radical intermediate. Spectra of organic radicals exhibiting both narrow lines and closely spaced hyperfine interactions have been recorded via RS-EPR. Well-resolved spectra can be recorded at a …

Gas Adsorption In Carbon Nanohorns: Equilibrium And Kinetics, Justin Matthew Petucci

Electronic Theses and Dissertations

A study of gas adsorption has been carried out with the focus of better understanding the relationships between the individual properties of the adsorbent/adsorbate (e.g. material structure, interactions, gas size and shape, etc.) and the overall adsorptive properties of the combined system (e.g. capacity, binding strength, equilibration time, etc.) as a function of thermodynamical variables. This is useful from the perspective of a comprehensive and fundamental understanding as well as for practical applications. The equilibrium regime of adsorption on carbon nanostructure materials (nanohorns, nanotubes, and graphite) is investigated using molecular statics (MS) and grand canonical monte carlo (GCMC) methods for …

Discrepancy Inequalities In Graphs And Their Applications, Adam Purcilly

Electronic Theses and Dissertations

Spectral graph theory, which is the use of eigenvalues of matrices associated with graphs, is a modern technique that has expanded our understanding of graphs and their structure. A particularly useful tool in spectral graph theory is the Expander Mixing Lemma, also known as the discrepancy inequality, which bounds the edge distribution between two sets based on the spectral gap. More specifically, it states that a small spectral gap of a graph implies that the edge distribution is close to random. This dissertation uses this tool to study two problems in extremal graph theory, then produces similar discrepancy inequalities based …

Iron In Urban Air Pollution, Joseph R. Salazar

Electronic Theses and Dissertations

The processes by which iron is solubilized are largely unknown. There are three hypotheses on how iron is soluble: 1) iron is thought to undergo an acid based solubilization process, 2) iron undergoes an oxidation/reduction process and reduces iron(III) to the more soluble iron(II), and 3) iron is bound to organic molecules, which solubilizes it. The 3 hypotheses on iron water-solubilized are explored by presenting two individual studies.

The first study investigates iron directly from car exhaust. Exhaust particles were collected from 32 vehicles. Iron solubility in these samples ranged from 0%-82% with the average iron solubility being 30%. X-ray …

Multidimensional Spectroscopy Of Mixed-Cation Perovskite Thin Films, Geoffrey Michael Diederich

Electronic Theses and Dissertations

Metal halide perovskite (MHP) thin films are currently undergoing an intense re- search thrust due to the excellent performance of MHP based photovoltaic (PV) devices, which have the potential to revolutionize the worlds energy production via a unique combination of low-cost fabrication and high power conversion efficiency (PCE). However, the vast majority of research is currently aimed at incremental improvements in device PCE, resulting in a body of work without the foundational understanding of the charge-carrier dynamics of the system upon photoexcitation.

This thesis begins with the development of a phase-modulated multidimensional coherent spectroscopy (PM-MDCS) experiment. PM-MDCS is an ultrafast …

Photoassisted Synthesis Of Complex Polyheterocycles Via Esipt-Driven Dearomative Intramolecular Cycloadditions, Dmitry Kuznetsov

Electronic Theses and Dissertations

This research is focused on the development of novel photoassisted synthetic methodologies that provide straightforward access to complex and diverse libraries of polyheterocycles from modularly assembled precursors. All methods are based on the dearomative cycloadditions between two components: (i) o-azaxylylenes, generated by the excited-state intramolecular proton transfer, and (ii) tethered arene groups.

We have demonstrated the synthetic utility of four new photoinduced processes that yield complex products amenable for post-photochemical modifications:

• [4+4] Cycloaddition of o-azaxylylenes to 1,3,4-oxadiazoles with subsequent dinitrogen extrusion. This reaction furnishes compounds outfitted with epoxide fragments, which undergo ring-openings with various nucleophiles; furthermore, the oxidized products …

Lewis Base Promoted Annulations Of Allenoates With 1,4-Naphthoquinones, Nicole Marie Shoup

Electronic Theses and Dissertations

Lewis base promoted cyclization of allenoates with electron deficient olefins open up the possibility to generate more complex organic structures. By generating more complex molecules, we are able to produce new compounds for biomedical and material applications. Both phosphine and amine Lewis bases offer interesting annulations with allenoates resulting in Lu or Morita–Baylis–Hillman type reactions. Once the Lewis base engages with the allenoate there can be several modes of nucleophilic reactivity that allows for the allenic ester to act as a two or three carbon synthon. The allenoate can then react with varying electron deficient olefins generating more complex molecules …

Barrier Graphs And Extremal Questions On Line, Ray, Segment, And Hyperplane Sensor Networks, Kirk Anthony Boyer

Electronic Theses and Dissertations

A sensor network is typically modeled as a collection of spatially distributed objects with the same shape, generally for the purpose of surveilling or protecting areas and locations. In this dissertation we address several questions relating to sensors with linear shapes: line, line segment, and rays in the plane, and hyperplanes in higher dimensions.

First we explore ray sensor networks in the plane, whose resilience is the number of sensors that must be crossed by an agent traveling between two known locations. The coverage of such a network is described by a particular tripartite graph, the barrier graph of the …

Development Of A Hek293 Cell Line To Show Inhibition Of Tau Aggregation, Justin Ray Shady

Electronic Theses and Dissertations

Intracellular deposition of aggregated tau is the hallmark of several different tauopathies, the most widespread of these being Alzheimer's disease. Tau is a highly soluble, intrinsically disordered, microtubule associated protein. Tau's native function is to stabilize microtubule formation in the axons of neurons. Post translational modification such as hyperphosphorylation as well as several familial mutations allow tau to nucleate and form fibrils. These fibrils can recruit healthy monomers onto their ends in a fashion described as template-assisted growth. Tau has 6 isoforms that vary by the inclusion or exclusion of two N-terminal repeats and the inclusion or exclusion of the …

Decidability For Residuated Lattices And Substructural Logics, Gavin St. John

Electronic Theses and Dissertations

We present a number of results related to the decidability and undecidability of various varieties of residuated lattices and their corresponding substructural logics. The context of this analysis is the extension of residuated lattices by various simple equations, dually, the extension of substructural logics by simple structural rules, with the aim of classifying simple equations by the decidability properties shared by their extensions. We also prove a number of relationships among simple extensions by showing the equational theory of their idempotent semiring reducts coincides with simple extensions of idempotent semirings. On the decidability front, we develop both semantical and syntactical …

Development And Characterization Of An Inexpensive Single-Particle Fluorescence Spectrometer For Detection And Classification Of Pollen And Other Bioaerosols, Benjamin E. Swanson

Electronic Theses and Dissertations

Atmospheric aerosols are ubiquitous throughout the Earth’s atmosphere and can be important with respect to environmental systems and human health. Pollen particles are a class of primary biological aerosol particles (PBAPs) that cost the United States billions of dollars a year in loss of productivity and healthcare costs due to allergy and respiratory effects. Traditional methods of pollen detection rely on collection and subsequent identification by visual microscopy, yet few measurement stations exist in the United States. As such, current pollen forecasting models have relatively high prediction uncertainty, especially in regions without sampling stations. Recently, laser-induced fluorescence instrumentation has been …

Brønsted Acid Catalyzed Carbon-Carbon Bond Forming Reactions, Jiangyue Miao

Electronic Theses and Dissertations

The focus of this research project lies in the development of new methodology in the field of Brønsted acid catalysis enabling rapid synthesis of medicinally relevant compounds. It is foreseen that small molecule sulfonic acids evaluated in this research project will unveil new asymmetric carbon carbon bond forming reactions between substrates hitherto unexplored with Brønsted acid catalysis. It has been established that strong Brønsted acids, such as phosphoric acids, are capable of mediating highly selective transformations operating through unique mechanistic manifolds.

Specific focus for the sulfonic acid catalysts was geared towards asymmetric coupling reactions with synthetically useful precursors such as …

Characterization Of The Atcc Α-Tc1-6 Pancreatic Alpha Cell Line To Study Glucagon Secretion, Larissa M. Ikenouye

Electronic Theses and Dissertations

The endocrine pancreas is responsible for maintaining glycemic equilibrium in the body. Given the importance of this blood-glucose homeostasis and the implication an unbalance has on Diabetes mellitus, the study of the glucose-sensing alpha and beta cells in the pancreas is a popular field for scientific researchers. In this study, we use immunofluorescence, qPCR analysis, intracellular calcium experiments, and biochemical glucagon secretion assays to determine if the commercially available tumor cell line clone, α-TC1-6 obtained from American Type Culture Collection, is an appropriate model system for glucagon secretion in pancreatic alpha cells. We confirm the production of the hormone peptide …

Banach Spaces From Barriers In High Dimensional Ellentuck Spaces, Gabriel Girón-Garnica

Electronic Theses and Dissertations

We construct new Banach spaces using barriers in high dimensional Ellentuck spaces following the classical framework under which a Tsirelson type norm is defined from a barrier in Ellentuck space. It is shown that these spaces contain arbitrary large copies of lⁿ_infinity and specific block subspaces isomorphic to l_p. We also prove that they are l_p-saturated and not isomorphic to each other. Finally, a study of alternative norms for our spaces is presented.

Quinolines: Novel Synthesis And Derivatives Of Heterocyclic Bioactive Agents, Ginelle A. Ramann

Electronic Theses and Dissertations

Tuberculosis is a pulmonary disease that has ravaged the world throughout history. Even today, many developed and developing countries are experiencing this plight, made worse by the scourge of antibiotic-resistant bacteria.

Mycobacterium tuberculosis's class II fructose-1,6-bisphosphate aldolase presents an interesting target in the design of new therapies against this disease. Previous attempts to construct an inhibitor to this enzyme fell short due to poor selectivity or poor cellular uptake. However a new class of noncompetitive inhibitor based on 8-hydoxyquinoline-2-carboxylic acid presents a fresh approach. Preliminary studies indicate various modifications to this scaffold could be beneficial to its inhibitory efficacy.

Modifications …

Dihedral-Like Constructions Of Automorphic Loops, Mouna Ramadan Aboras

Electronic Theses and Dissertations

In this dissertation we study dihedral-like constructions of automorphic loops. Automorphic loops are loops in which all inner mappings are automorphisms. We start by describing a generalization of the dihedral construction for groups. Namely, if (G , +) is an abelian group, m > 1 and α ∈2 Aut(G ), let Dih(m, G, α) on Z_m × G be defined by

(i, u )(j, v ) = (i + j , ((-1)^j u + v )α^ij ).

We prove that the resulting loop is automorphic if and only if m = 2 …

Spectropolarimetry Of Epsilon Aurigae: Probing Stellar And Disk Atmospheres, Kathleen M. Geise

Electronic Theses and Dissertations

The bright eclipsing binary system epsilon Aurigae offers a unique opportunity to uncover physical mechanisms contributing to disk formation and evolution and to explore the relationship between photospheric anisotropies, stellar pulsation and mass loss. This research contributes to our understanding of stellar evolution in the context of binary stars. The research also offers the opportunity to investigate disk formation and evolution significant to our understanding of protoplanetary disks now seen in many star systems. Lastly, the project considers radiative transfer of polarized light that contributes to diverse fields such as atmospheric studies of exoplanets.

My objective is to understand the …

Economic And Traffic Impacts Following The Installation Of New Bicycle Facilities: A Denver Case Study, Stephen Antonio Rijo

Electronic Theses and Dissertations

America is currently experiencing a unique departure from the historical vehicle miles traveled (VMT) trend, often referred to as "peak travel." Decreasing VMT numbers suggest that the US is currently experiencing an unprecedented change in the way individuals utilize transportation systems. The following research calls on a need to understand the importance of improving alternative transportation infrastructure, namely cycling facilities, and how this impacts local businesses and their communities. This research informs the overarching question, "What are the economic and traffic impacts of cycling facilities?" A mixed methods analysis of retail sales tax, traffic count, and RTD transit access data …

Electron Paramagnetic Resonance Imaging And Probes, Hanan B. Elajaili

Electronic Theses and Dissertations

EPR imaging at low frequency is an important method to measure in vivo physiology. Because native paramagnetic species exist at low concentrations in living systems, exogenous paramagnetic species are needed for in vivo EPR. Selection of the appropriate EPR probe allows the experimenter to obtain information about the environment of the imaged probe and its spatial distribution. Relaxation times for several nitroxide radicals were studied with the aim of understanding the relaxation mechanisms, which provides criteria for design of suitable in vivo imaging probes.

Amino-substituted trityl radicals have the potential to monitor pH in vivo, and the suitability for this …

Molecular Encapsulation In Kinetically Trapped, Hydrogen-Bonded Pyrogallolarene Hexamers, Jennifer Christine Chapin Lake

Electronic Theses and Dissertations

Pyrogallolarene and resorcinarene hexamers are hydrogen-bonded capsules that self-assemble in the solid state and can be studied in gaseous and solution phases. Guest loading within pyrogallolarene hexamers in solution has primarily been comprised of solvent molecules with some tertiary amines. A novel solvent-free method for loading guests into the interior of the hexamer has been shown to be effective for encapsulation of a variety of molecules. Aromatic and aliphatic compounds have particularly high guest loading within the hexamers and often result in kinetically stable guest-filled capsules in solution.

Kinetic studies of the encapsulation complexes of anthracene, biphenyl, fluoranthene, fluorene, naphthalene, …

Dust And Pleistocene Ice Ages: Eolian Sediments And Climate Change At Ziegler Reservoir, Snowmass Village, Colorado, Jeffrey S. Honke

Electronic Theses and Dissertations

The discovery of the Ziegler Reservoir fossil site near Snowmass Village, Colorado presents an opportunity to examine subalpine ecosystem response, during a relatively unknown period, in which climate conditions were similar to present. Finegrained sediments at Ziegler Reservoir represent continuous deposition between ~140–55 ka (thousand years before present), spanning the close of the Bull Lake glacial period [marine isotope stage (MIS) 6], the Sangamon interglacial (MIS 5) and the early stages of the Pinedale (early Wisconsin) glacial period (MIS 4). Ziegler Reservoir is positioned on top of a ridge, at an elevation of 2705 m, and has a small watershed …