Physical Sciences and Mathematics Commons^™

Improvement Of Ifng Elispot Performance Following Overnight Resting Of Frozen Pbmc Samples Confirmed Through Rigorous Statistical Analysis, Radleigh Santos, Alcinette Bunying, Nazila Sabri, John Yu, Anthony Gringeri, James Bender, Sylvia Janetzki, Clemencia Pinilla, Valeria A. Judkowski

Mathematics Faculty Articles

Immune monitoring of functional responses is a fundamental parameter to establish correlates of protection in clinical trials evaluating vaccines and therapies to boost antigen-specific responses. The IFNg ELISPOT assay is a well-standardized and validated method for the determination of functional IFNg-producing T-cells in peripheral blood mononuclear cells (PBMC); however, its performance greatly depends on the quality and integrity of the cryopreserved PBMC. Here, we investigate the effect of overnight (ON) resting of the PBMC on the detection of CD8-restricted peptide-specific responses by IFNg ELISPOT. The study used PBMC from healthy donors to evaluate the CD8 T-cell response to five pooled …

Comparative Population Genetics And Evolutionary History Of Two Commonly Misidentified Billfishes Of Management And Conservation Concern, Andrea M. Bernard, Mahmood S. Shivji, Eric Prince, Fabio H.V. Hazin, Freddy Arocha, Andres Domingo, Kevin A. Feldheim

Marine & Environmental Sciences Faculty Articles

Background: Misidentifications between exploited species may lead to inaccuracies in population assessments, with potentially irreversible conservation ramifications if overexploitation of either species is occurring. A notable showcase is provided by the realization that the roundscale spearfish (Tetrapturus georgii), a recently validated species, has been historically misidentified as the morphologically very similar and severely overfished white marlin (Kajikia albida) (IUCN listing: Vulnerable). In effect, no information exists on the population status and evolutionary history of the enigmatic roundscale spearfish, a large, highly vagile and broadly distributed pelagic species. We provide the first population genetic evaluation of the …

Reproductive Parameters Of Two Coastal Pelagic Fishes Off Southeast Florida: Blackfin Tuna Thunnus Atlanticus And Little Tunny Euthynnus Alletteratus, Sonia Ahrabi-Nejad

HCNSO Student Theses and Dissertations

The ability to manage a fish stock relies on an understanding of life history characteristics and basic biology of the species. Numerous age-growth studies are facilitated by the relative ease of ageing fishes through hard-part analyses. Determining reproductive parameters for fish populations is equally important for stock assessments and management, and histological examination of gonads provides the most accurate determination of fecundity and spawning periods. Coastal pelagic fishes are often targeted commercially and recreationally due to their easy access by private vessels. However, there are few studies researching the biology and reproduction of recreational fishes in the waters of Southeastern …

Changes In Coral Community Composition At Devil's Crown, Galapagos Islands, Ecuador: A 7,700 Year Perspective, Katharine Jane Hendrickson

HCNSO Student Theses and Dissertations

Coral mortality caused by El Niño–Southern Oscillation (ENSO) activity and its related disturbances has been researched throughout the Eastern Pacific. In the past three decades, disturbances related to the El Niño–Southern Oscillation (ENSO) have been shown to influence coral growth in the Eastern Pacific. In the Galápagos Islands, Ecuador, more than 97% of corals experienced mortality after the severe 1982-1983 ENSO episode. However, two of the most dominant coral species found in a coral community adjacent to Devil’s Crown; Psammocora stellata and Diaseris distorta survived this severe ENSO event. By reconstructing sediment cores of the coral community, this study assessed …

Distribution, Growth, And Impact Of The Coral-Excavating Sponge, Cliona Delitrix, On The Stony Coral Communities Offshore Southeast Florida, Ari Halperin

HCNSO Student Theses and Dissertations

Bioerosion is a major process that affects the carbonate balance on coral reefs, and excavating sponges from the genus Cliona are some of the most important bioeroders on Caribbean reefs. The orange boring sponge, Cliona delitrix, is an abundant excavating sponge offshore southeast Florida that frequently colonizes dead portions of live stony corals, killing live coral tissue as it grows. With the recent decline in coral cover attributed to combined environmental and anthropogenic stressors, the increasing abundance of excavating sponges poses yet another threat to the persistence of Caribbean coral reefs.

In the first part of this study, I …

Assessment Of Macroinvertebrate Communities And Heavy Metal Contamination Along The Intracoastal Waterway In Fort Lauderdale, Florida, Robert Bernhard

HCNSO Student Theses and Dissertations

Sediments from four areas adjacent to marinas and a background site in the Intracoastal Waterway were assessed for macroinvertebrate composition and heavy metal contamination. Sediment core samples were collected in 2004 and 2005 for analyses of macroinvertebrate composition and sediment grain size. Additional sediment samples were collected in 2005 for chemical analyses of metals (Al, As, Cd, Cr, Cu, Fe, Pb, Hg, Ni and Zn). MANOVA and dendograms using Bray-Curtis similarity matrices grouped the sites into two clusters: the 3 sites closest to the New River formed one group, and the two end sites formed the other. The sites nearest …

Book Review - Wild Connection: What Animal Courtship And Mating Tell Us About Human Relationships, J. Matthew Hoch

Marine & Environmental Sciences Faculty Articles

No abstract provided.

Alternative Approaches To Correction Of Malapropisms In Aiml Based Conversational Agents, Walter A. Brock

CCE Theses and Dissertations

The use of Conversational Agents (CAs) utilizing Artificial Intelligence Markup Language (AIML) has been studied in a number of disciplines. Previous research has shown a great deal of promise. It has also documented significant limitations in the abilities of these CAs. Many of these limitations are related specifically to the method employed by AIML to resolve ambiguities in the meaning and context of words. While methods exist to detect and correct common errors in spelling and grammar of sentences and queries submitted by a user, one class of input error that is particularly difficult to detect and correct is the …

Factors Impacting Information Security Noncompliance When Completing Job Tasks, Martha Nanette Harrell

CCE Theses and Dissertations

Work systems are comprised of the technical and social systems that should harmoniously work together to ensure a successful attainment of organizational goals and objectives. Information security controls are often designed to protect the information system and seldom consider the work system design. Using a positivist case study, this research examines the user's perception of having to choose between completing job tasks or remaining compliant with information security controls. An understanding of this phenomenon can help mitigate the risk associated with an information system security user's choice. Most previous research fails to consider the work system perspective on this issue. …

Comment On “Chemically Mediated Behavior Of Recruiting Corals And Fishes: A Tipping Point That May Limit Reef Recovery”, Andrew H. Baird, Vivian R. Cumbo, Joana Figueiredo, Saki Harii, Tom Hata, Joshua S. Madin

Marine & Environmental Sciences Faculty Articles

Dixson et al. (2014) report that coral larvae navigate towards chemical cues associated with healthy reefs and avoid cues from degraded reefs. However, the swimming capabilities of coral larvae and well-established patterns of recruitment and reef hydrodynamics indicate that coral larvae will not be able to use these cues to recruit to healthy reefs. Perfuming degraded reefs, as suggested by Dixson et al (2014), will not enhance recovery rather it will distract from the difficult task of reducing fishing effort and improving water quality.

Temperature-Induced Viral Resistance In Emiliania Huxleyi (Prymnesiophyceae), B. Jacob Kendrick, Giacomo R. Ditullio, Tyler Cyronak, James M. Fulton, Benjamin A. S. Van Mooy, Kay D. Bidle

Marine & Environmental Sciences Faculty Articles

Annual Emiliania huxleyi blooms (along with other coccolithophorid species) play important roles in the global carbon and sulfur cycles. E. huxleyi blooms are routinely terminated by large, host-specific dsDNA viruses, (Emiliania huxleyi Viruses; EhVs), making these host-virus interactions a driving force behind their potential impact on global biogeochemical cycles. Given projected increases in sea surface temperature due to climate change, it is imperative to understand the effects of temperature on E. huxleyi’s susceptibility to viral infection and its production of climatically active dimethylated sulfur species (DSS). Here we demonstrate that a 3°C increase in temperature induces EhV-resistant phenotypes …

Information System Security Commitment: A Study Of External Influences On Senior Management, Kevin Andrew Barton

CCE Theses and Dissertations

This dissertation investigated how senior management is motivated to commit to information system security (ISS). Research shows senior management participation is critical to successful ISS, but has not explained how senior managers are motivated to participate in ISS. Information systems research shows pressures external to the organization have greater influence on senior managers than internal pressures. However, research has not fully examined how external pressures motivate senior management participation in ISS. This study addressed that gap by examining how external pressures motivate senior management participation in ISS through the lens of neo-institutional theory. The research design was survey research. Data …

Serving Military Families: Perceptions Of Educational Counseling In A Virtual Environment, Taryn Stevenson

CCE Theses and Dissertations

The advances in communication technology over the past 20 years have significant implications for the delivery of psycho-educational therapeutic services to populations that have been historically underserved due to remote locations lacking trained providers. One such population is military families, who also suffer from a negative stigma of asking for outside help or education for personal growth. This population also faces increasing mental health needs due to military deployment in Operation Iraqi Freedom (OIF) and Operation Enduring Freedom (OEF). These operations have increased the number of returning service members who have been physically and mentally injured. The effect that these …

Marginal Coral Populations: The Densest Known Aggregation Of Pocillopora In The Galápagos Archipelago Is Of Asexual Origin, Iliana B. Baums, Meghann Devlin-Durante, Beatrice A. A. Laing, Joshua S. Feingold, Tyler B. Smith, Andrew Bruckner, Joao Monteiro

Marine & Environmental Sciences Faculty Articles

Coral populations at distributional margins frequently experience suboptimal and variable conditions. Recurrent El Niño-Southern Oscillation (ENSO) warming events have caused extensive mortality of reef-building corals in the Eastern Pacific, and particularly impacted branching pocilloporid corals in the Galápagos Islands. Pocillopora spp. were previously more common and formed incipient reefs at several locations in the archipelago but now occur as scattered colonies. Here, we report an unusually concentrated aggregation of colonies and evaluate their current genetic diversity. In particular we focus on a large population of 1614 live Pocillopora colonies found in a volcanic lagoon along the southern shore of Isabela …

A Method To Reduce The Cost Of Resilience Benchmarking Of Selfadaptive Systems, Steve Hernandez

CCE Theses and Dissertations

Ensuring the resilience of self-adaptive systems used in critical infrastructure systems is a concern as their failure has severe societal and financial consequences. The current trends in the growth of the scale and complexity of society's workload demands and the systems built to cope with these demands increases the anxiety surrounding service disruptions. Self-adaptive mechanisms instill dynamic behavior to systems in an effort to improve their resilience to runtime changes that would otherwise result in service disruption or failure, such as faults, errors, and attacks. Thus, the evaluation of a self-adaptive system's resilience is critical to ensure expected operational qualities …

Gwatch: A Web Platform For Automated Gene Association Discovery Analysis, Anton Svitin, Sergey Malov, Nikolay Cherkasov, Paul Geerts, Mikhail Rotkevich, Pavel Dobrynin, Andrey Shevchenko, Li Guan, Jennifer L. Troyer, Sher L. Hendrickson, Holli Hutcheson Dilks, T. K. Oleksyk, Sharyne Donfield, Edward Gomperts, Douglas A. Jabs, Efe Sezgin, Mark Van Natta, P. Richard Harrigan, Zabrina L. Brumme, Stephen J. O'Brien

Biology Faculty Articles

Background: As genome-wide sequence analyses for complex human disease determinants are expanding, it is increasingly necessary to develop strategies to promote discovery and validation of potential disease-gene associations.

Findings: Here we present a dynamic web-based platform – GWATCH – that automates and facilitates four steps in genetic epidemiological discovery: 1) Rapid gene association search and discovery analysis of large genome-wide datasets; 2) Expanded visual display of gene associations for genome-wide variants (SNPs, indels, CNVs), including Manhattan plots, 2D and 3D snapshots of any gene region, and a dynamic genome browser illustrating gene association chromosomal regions; 3) Real-time validation/replication …

Unsupervised Learning Trojan, Arturo Geigel

CCE Theses and Dissertations

This work presents a proof of concept of an Unsupervised Learning Trojan. The Unsupervised Learning Trojan presents new challenges over previous work on the Neural network Trojan, since the attacker does not control most of the environment. The current work will presented an analysis of how the attack can be successful by proposing new assumptions under which the attack can become a viable one. A general analysis of how the compromise can be theoretically supported is presented, providing enough background for practical implementation development. The analysis was carried out using 3 selected algorithms that can cover a wide variety of …

Habitat And Seasonal Distribution Of The North American River Otter (Lontra Canadensis) And Vertebrate Species Assemblages In Two Protected Areas Of The Florida Everglades, Catherine Faye Hamilton

HCNSO Student Theses and Dissertations

The Florida Everglades ecosystem is threatened by human development, increased pollution, freshwater scarcity, and invasive species; factors that have negatively impacted the Everglades and native species health and populations. Man-made canals and levies have redirected the natural flow of fresh water from Lake Okeechobee into the Florida Everglades, starving central and south Florida ecosystems of necessary fresh water and nutrients. Through the efforts of the Comprehensive Everglades Restoration Project (CERP), freshwater is being redirected back into central and south Florida, returning the sheet flow of water back into the Everglades. Monitoring species abundance in the Everglades is a beneficial conservational …

The Effects Of An Extended Power Plant Shutdown On The Florida Manatee (Trichechus Manatus Latirostris) In Port Everglades, Florida, Christopher Grissett

HCNSO Student Theses and Dissertations

Florida manatees (Trichechus manatus latirostris) seek out warmer waters during winter months when ambient water temperatures drop below 20 degrees Celsius. Over time, manatees have discovered artificial warm water sites from power plant discharges in addition to natural sites such as springs and passive thermal refugia (PTRs). The Florida Power and Light (FPL) Port Everglades power plant in Broward County is one such artificial warm water refuge used by manatees. This plant was shutdown on July 16, 2013, and is expected to remain off line for at least three years during demolition and construction of a new facility. …

A Causal Model To Predict Organizational Knowledge Sharing Via Information And Communication Technologies, Simon Cleveland

CCE Theses and Dissertations

Knowledge management literature identifies numerous barriers that inhibit employees' knowledge seeking and knowledge contributing practices via information and communication technologies (ICTs). Presently, there is a significant gap in the literature that explains what factors promote common knowledge sharing barriers. To bridge this gap, this study examined two research questions: 1) What are the potential factors that contribute to the commonly accepted barriers to knowledge sharing?, and 2) How do these factors impact employees' use of ICTs for knowledge seeking and knowledge contributing? Literature review of 103 knowledge management articles identified three major barriers to knowledge sharing practices (lack of time, …

An Investigation Of The Dayton Regional Stem School Public-Private Partnerships, Kimberly S. Poole

CCE Theses and Dissertations

This dissertation study documents in-depth the exploration of the Public Private Partnerships (PPPs) between the Dayton Regional STEM School (DRSS) and their industry partners as well as the establishment of a framework for evaluating and assessing PPPs. The public-private partnership agreements were studied in order to answer the over-arching research question: How is an effective public-private partnership established, assessed, and evaluated in education? A descriptive case study methodology was used to study DRSS' public-private partnership agreements to determine if goals and objectives were established and whether or not the partnerships met those goals and objectives. This case study also included …

Using Class Interfaces And Mock Objects To Unit Test Aspects, Michael Bryan Snider

CCE Theses and Dissertations

In object oriented programming (OOP) class objects are individual units of code that encapsulate the desired functionality of each object. AOP is an attempt to handle the cross-cutting concerns that represent functionality needed by a class, but is not specific to that class. The cross-cutting functionality is implemented in AOP by using a class-like structure, the aspect. Aspects do not have their own context and as such are dependent upon other objects for their context. By not having their own context it is difficult to test the functionality of aspects. This study investigated the effectiveness of using class interfaces and …

Measuring The Socio-Economic Characteristics Of A Lionfish Derby Event, Adam Nardelli, Stephanie Green, L. Akins, David W. Kerstetter

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

No abstract provided.

Security Policies That Make Sense For Complex Systems: Comprehensible Formalism For The System Consumer, Rhonda R. Henning

CCE Theses and Dissertations

Information Systems today rarely are contained within a single user workstation, server, or networked environment. Data can be transparently accessed from any location, and maintained across various network infrastructures. Cloud computing paradigms commoditize the hardware and software environments and allow an enterprise to lease computing resources by the hour, minute, or number of instances required to complete a processing task. An access control policy mediates access requests between authorized users of an information system and the system's resources. Access control policies are defined at any given level of abstraction, such as the file, directory, system, or network, and can be …

Designing For Statistical Reasoning And Thinking In A Technology-Enhanced Learning Environment, Wendy Tu

CCE Theses and Dissertations

Difficulties in learning and understanding statistics in college education have led to a reform movement in statistics education in the early 1990s. Although much work has been done, there is more work that needs to be done in statistics education. The progress depends on how well the educators bring interesting real-life data into the classroom.

The goal was to understand how course design based on First Principles of Instruction could facilitate tertiary-level students' conceptual understanding when learning introductory statistics in a technology-enhanced learning environment. An embedded single descriptive case design was employed to investigate how integrating technology and real data …

Secure Mobile Deployment Of Nfl Training Materials, Alexander R. Corris

CCE Theses and Dissertations

The problem addressed is the lack of empirical research describing the delivery of individualized learning material in a secure and mobile manner. The goal was to investigate the effectiveness of deploying training materials to National Football League (NFL) players during a recent NFL season. Over the past few seasons, NFL teams have started to deliver player training material to mobile devices. The training material is sensitive and includes planning documents for upcoming games. An effort was made to survey a representative at each of the 32 NFL teams in order to gain insight on effectiveness, security, and process. Nearly half …

Performance Comparison Of Projective Elliptic-Curve Point Multiplication In 64-Bit X86 Runtime Environment, Ninh Winson

CCE Theses and Dissertations

For over two decades, mathematicians and cryptologists have evaluated and presented the theoretical performance of Elliptic-curve scalar point-multiplication in projective geometry. Because computation in projective domain is composed of a wide array of formulations and computing optimizations, there is not a comprehensive performance comparison of point-multiplication using projective transformation available to verify its realistic efficiency in 64-bit x86 computing platforms. Today, research on explicit mathematical formulations in projective domain continues to excel by seeking higher computational efficiency and ease of realization. An explicit performance evaluation will help implementers choose better implementation methods and improve Elliptic-curve scalar point-multiplication. This paper was …

Understanding Usability-Related Information Security Failures In A Healthcare Context, Edward D. Boyer

CCE Theses and Dissertations

This research study explores how the nature and type of usability failures impact task performance in a healthcare organization. Healthcare organizations are composed of heterogeneous and disparate information systems intertwined with complex business processes that create many challenges for the users of the system. The manner in which Information Technology systems and products are implemented along with the overlapping intricate tasks the users have pose problems in the area of usability. Usability research primarily focuses on the user interface; therefore, designing a better interface often leaves security in question. When usability failures arise from the incongruence between healthcare task and …

Assistive Technologies Used By Students With Asperger’S Syndrome To Improve Performance In The General Education Classroom, Sara (Beth) Cardwell Foreman

CCE Theses and Dissertations

The No Child Left Behind Act requires all students; regardless of poverty level, learning disability, limited English proficiency, or racial/ethnic origins; to be in general education classrooms meeting high standards of proficiency in reading/language arts and mathematics by 2013-14. Meeting high standards of proficiency in a general education classroom is particularly challenging for students diagnosed with pervasive developmental disorders, such as Asperger's syndrome. Limited interests, inflexible language, and social skills deficits without a history of cognitive or language delays before the age of three characterize the disorder. Other symptoms, such as clumsiness, unusual sensory reactions, and talk in unusual ways …

An Investigation Of The Key Factors That Affect The Adoption Of Smartphones In Global Midmarket Professional Service Firms, Mark S. Kocour

CCE Theses and Dissertations

The evolution and proliferation of mobile devices (m-devices) in the workplace have been rapid. In comparison to conventional services provided by mobile phones (m-phones), smartphones feature sophisticated functionality, such as Internet access, video/audio streaming, and business productivity applications. As a consequence of increased demand for smartphones in the workplace, an understanding of the factors that determine the decision to adopt smartphones in business settings is necessary. The goal of this investigation was to identify the key factors that have an impact on the adoption of smartphones.

This dissertation investigation provided an understanding of the key factors that affect the adoption …