Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Analytical chemistry (20)
- Organic chemistry (20)
- Synthesis (18)
- Microfluidics (17)
- Nanoparticles (16)
-
- Mass spectrometry (12)
- Capillary electrophoresis (11)
- GUMBOS (11)
- Atomic force microscopy (10)
- Fluorescence (10)
- Intermetallics (10)
- Hydroformylation (9)
- NMR (9)
- Particle lithography (9)
- Spectroscopy (9)
- Self-assembly (8)
- AFM (7)
- Catalysis (7)
- Ionic liquids (7)
- Polymeric surfactants (7)
- EPFRs (6)
- Electrophoresis (6)
- Flux growth (6)
- Liposomes (6)
- Physical chemistry (6)
- Porphyrins (6)
- Protein (6)
- X-ray diffraction (6)
- Analytical chemistry; Organic chemistry (5)
- Conjugated polymer (5)
- Publication Year
- Publication
- Publication Type
Articles 1 - 30 of 573
Full-Text Articles in Physical Sciences and Mathematics
Simulations Of Intruder-Free X-Ray Transient Absorption And Time-Resolved X-Ray Scattering For Probing Attosecond Electron Dynamics, Mengqi Yang
LSU Doctoral Dissertations
The motion of electrons plays a fundamental role in both physics and chemistry, and capturing such dynamics requires the ability to resolve changes at the attosecond timescale, which is enabled by the advent of ultrashort laser pulses. This dissertation aims to facilitate the interpretation of ultrafast electron dynamics and attosecond spectroscopy by real-time time-dependent density functional theory (RT-TDDFT) simulations. The first part of this dissertation focuses on improving the simulation of X-ray transient absorption spectra (XTAS) with Gaussian basis sets in RT-TDDFT by applying a filter to the transition dipole matrix. Due to the spatial limitation of atom-centered Gaussian functions, …
Nanosecond Infrared Laser Ablation Dynamics, Achala Priyadarshani Deenamulla Kankanamalage
Nanosecond Infrared Laser Ablation Dynamics, Achala Priyadarshani Deenamulla Kankanamalage
LSU Doctoral Dissertations
Laser ablation dynamics encompasses studies of fundamental physical processes of mid infrared laser ablation. Understanding the mechanisms of IR laser desorption and ionization can lead to improvements in laser ablation-based techniques and expansion of their applications. Control of material removal ensures both accuracy and precision of the laser ablation-based techniques. The laser ablation mechanism in the studied wavelength region, is a process of water vaporization and photothermal disruption of tissue. Glycerol was used as the ablation target to establish the methods. Experiments were first aimed at developing methods to monitor material removal during ablation using a 2.94 µm wavelength mid-IR …
Impact Of The Choice Of Acidic Buffers On The Electrochemical Reduction Of Hexavalent Chromium In Water, Devin D. Meche
Impact Of The Choice Of Acidic Buffers On The Electrochemical Reduction Of Hexavalent Chromium In Water, Devin D. Meche
Honors Theses
No abstract provided.
Polymer Mimetics For Soil Modeling And Detection Of Biomarkers, Md Ragib Hasan
Polymer Mimetics For Soil Modeling And Detection Of Biomarkers, Md Ragib Hasan
LSU Doctoral Dissertations
The population of the world is increasing day by day and is expected to reach 9.8 billion by the year 2050. The ever-increasing demand for agricultural products is putting an unprecedented strain on the world's soils as the human population continues to expand. Soil degradation caused by over-farming and the agrochemicals (fertilizers, pesticides, etc.) used in agriculture is a growing problem, although its causes remain murky. In addition, little is understood about the molecular-level interactions of substances that are subsequently introduced to soils, such as agricultural chemicals (ACs). Therefore, it is expected that these constraints may be circumvented by the …
Development Of 1,2-Cis-Selective Glycosylation Protocols And Multistep Synthesis Of Glycans From Acinetobacter Baumannii., Dancan Kamau Njeri
Development Of 1,2-Cis-Selective Glycosylation Protocols And Multistep Synthesis Of Glycans From Acinetobacter Baumannii., Dancan Kamau Njeri
LSU Doctoral Dissertations
Access to various oligosaccharides has been made possible by glycosylation reactions. This is mainly through chemical synthesis and enzymatic approaches. Herein, I talk about glycosylation through chemical synthesis. In general, glycosylation reactions lead to a mixture of 1,2-cis and 1,2-trans stereoisomers. Stereoselective access to 1,2-trans isomer is highly developed; it efficiently relies on neighboring group participation. However, efficient access to 1,2-cis selective glycosylation is still a work in progress. In Chapter 1, I highlight notable efforts developed so far that address the 1,2-cis selectivity challenge in glycosylation.
In Chapter 2, I demonstrate how I improved …
A New Insight Into Fungal Cell Wall Architecture By Functional Genomics And Solid-State Nmr Along With Recent Advancements In Dynamic Nuclear Polarization For Analyzing Biomolecules, Arnab Chakraborty
LSU Master's Theses
This dissertation summarizes the findings related to the way by which supramolecular architecture of fungal cell wall changes with genetic mutation, dispensing genes responsible for biosynthesis of cell wall polysaccharides. This is necessary because without perfect picture of how supramolecular assembly changes with genetic mutation it is hard to assess new anti-fungal targets. Alongside this we have highlighted how recent advancement into Dynamic Nuclear Polarization (DNP) methods improved characterization of biomolecules both in case of labeled and unlabeled samples.
First study utilized Solid-state NMR (SSNMR) which is a non-destructive technique hence enabled us for the first time to deduce how …
New Synthetic Reactions Enabled By Protected Oxyallyl And Amidoallyl Cations, Binod Nepal
New Synthetic Reactions Enabled By Protected Oxyallyl And Amidoallyl Cations, Binod Nepal
LSU Doctoral Dissertations
This dissertation entails design and development of two new synthetic methodologies made possible through the generation of cationic species, namely oxyallyl and amidoallyl cations. Chapter 1 involves literature survey on generation and trapping of oxyallyl cations. Mostly known for cycloaddition reactions with these elusive species, the most recent approach of direct nucleophilic capture of oxyallyl cations are discussed. Apt design of substrates and reaction conditions by Kartika Group to access a-electrophilic carbon towards the synthesis of a variety of value-added compounds, i.e., 1,4- and 1,6-dicarbonyls, carbazoles, densely substituted pyrroles and furans are elaborated.
Chapter 2 focuses on our discovery of …
Competitive Sorption And Transport Of Heavy Metals In Soil And Mn Oxide-Coated Sand, Joshua Tyler Padilla
Competitive Sorption And Transport Of Heavy Metals In Soil And Mn Oxide-Coated Sand, Joshua Tyler Padilla
LSU Doctoral Dissertations
This study investigated the competitive sorption of Ni, Pb, and Zn as well as synergistic interactions between Ni and phosphate (P) in soils and Mn oxide-coated sand. During stirred-flow experiments, the sorption of heavy metal cations by Olivier soil was mutually reduced when applied simultaneously. When applied consecutively, the cation of higher affinity was able to displace the cation of lower affinity from the soil surface, however, no evidence for the reverse case was observed. A novel general exchange kinetics model was able to correctly describe such displacement. Competition between Ni and Zn mutually increased mobility in Olivier and Windsor …
Observing Reactive Events At The Aqueous Graphene Oxide Interface Using Deep Neural Network Potentials, Lukas Kim
Honors Theses
No abstract provided.
Mathematica Assessment Of Spatial Reasoning Development After Virtual Reality Collaboration, Arthur Odenheimer
Mathematica Assessment Of Spatial Reasoning Development After Virtual Reality Collaboration, Arthur Odenheimer
Honors Theses
No abstract provided.
Expression And Purification Of Tag-Free Chlamydia Trachomatis Scc4 For Improved Nmr Structural Studies, Peter Howard
Expression And Purification Of Tag-Free Chlamydia Trachomatis Scc4 For Improved Nmr Structural Studies, Peter Howard
Honors Theses
No abstract provided.
Synthesis Of Thiol-Acrylate Hydrogels For 3d Cell Culture And Microfluidic Applications, Anowar Hossain Khan
Synthesis Of Thiol-Acrylate Hydrogels For 3d Cell Culture And Microfluidic Applications, Anowar Hossain Khan
LSU Doctoral Dissertations
Globally cell culture is an $18.98 billion industry as of 2020, with an 11.6 percent annual growth rate. Drug discovery has an estimated worth of $69.8 billion in 2020 and is predicted to grow to $110.4 billion by 2025. Three-dimensional (3D) cell culture of cancer cells is one of the rapidly growing felids since it better recapitulates in vivo conditions compared to two-dimensional (2D) models. However, it is challenging to grow 3D tumor spheroids outside the body, and some of the existing technology can generate these spheroids outside the human body but poorly mimic in vivo tumor models. Therefore, there …
Computational Study Of The Reactions Of Heteroatomic Compounds On Ceo2, Suman Bhasker Ranganath
Computational Study Of The Reactions Of Heteroatomic Compounds On Ceo2, Suman Bhasker Ranganath
LSU Doctoral Dissertations
The mechanisms of ambient-temperature reactions of heteroatomic compounds catalyzed by ceria (CeO2), an archetypical reducible oxide, for enzyme mimetics, environmental protection, and chemical synthesis are investigated in this dissertation using theoretical methods. CeO2 is modeled with thermodynamically stable low-index surfaces exposed by commonly studied ceria thin films and nano particles. To understand phosphatase-like dephosphorylation activity, stable adsorption states and surface reactions of model phosphates are examined. Binding of the central P-atom to surface lattice oxygen (Olatt) supplemented by phosphoryl O-Ce interaction is the only stable adsorption state for the un-dissociated molecule. Deprotonation of phosphate monoesters, …
Kinetics, Characterization, And Applications In The Development Of Next-Generation Cure-On-Demand Polymeric Materials Through Frontal Polymerization, Daniel Paul Gary
Kinetics, Characterization, And Applications In The Development Of Next-Generation Cure-On-Demand Polymeric Materials Through Frontal Polymerization, Daniel Paul Gary
LSU Doctoral Dissertations
Frontal polymerization (FP) is a process in which a front propagates in a localized reaction zone converting monomer into polymer. This work explored the kinetics and applications of FP for the development of cure-on-demand materials.
The kinetic effects of fillers on frontal polymerization have not been thoroughly explored. In Chapter 2, various fillers were used, and their effects on front velocity and front temperature were determined. Clay minerals are primarily used, but the thermal conductive effects of milled carbon fiber were also explored. It was found that some fillers inhibit frontal polymerization through radical scavenging, while others increased the front …
Infrared Investigation Of Lithium Ion Electrolytes: Characterization Of Structure And Dynamics Via Linear And Nonlinear Spectroscopy, Jeramie Christopher Rushing
Infrared Investigation Of Lithium Ion Electrolytes: Characterization Of Structure And Dynamics Via Linear And Nonlinear Spectroscopy, Jeramie Christopher Rushing
LSU Doctoral Dissertations
Lithium ion batteries are widely employed in energy storage, but the connection between the molecular interactions in their electrolytes and the macroscopic properties remains elusive. Across three vastly different electrolytes, speciation and dynamics were studied via linear and nonlinear infrared spectroscopy to shed light on this relationship. The impact of mixed solvation on ionic speciation was studied from the perspective of the anion, which revealed a significant energetic favorability for the formation of contact ion pairs in linear carbonate solvents over cyclic carbonates. Infrared spectroscopy and density functional theory calculations described a complete inversion of the speciation due to solvent …
Whole-Genome Sequencing And Rna-Seq Reveal Differences In Genetic Mechanism For Flowering Response Between Weedy Rice And Cultivated Rice, Richard S. Garcia, Sapphire Coronejo, Jonathan Concepcion, Prasanta K. Subudhi
Whole-Genome Sequencing And Rna-Seq Reveal Differences In Genetic Mechanism For Flowering Response Between Weedy Rice And Cultivated Rice, Richard S. Garcia, Sapphire Coronejo, Jonathan Concepcion, Prasanta K. Subudhi
Faculty Publications
Flowering is a key agronomic trait that influences adaptation and productivity. Previous studies have indicated the genetic complexity associated with the flowering response in a photoinsensitive weedy rice accession PSRR-1 despite the presence of a photosensitive allele of a key flowering gene Hd1. In this study, we used whole-genome and RNA sequencing data from both cultivated and weedy rice to add further insights. The de novo assembly of unaligned sequences predicted 225 genes, in which 45 were specific to PSRR-1, including two genes associated with flowering. Comparison of the variants in PSRR-1 with the 3K rice genome (RG) dataset identified …
Docking And Molecular Dynamic Of Microalgae Compounds As Potential Inhibitors Of Beta-Lactamase, Roberto Pestana-Nobles, Yani Aranguren-Diaz, Elwi Machado-Sierra, Juvenal Yosa, Nataly J. Galan-Freyle,, Laura X. Sepulveda-Montano, Daniel G. Kuroda, Leonardo C. Pacheco-Londono
Docking And Molecular Dynamic Of Microalgae Compounds As Potential Inhibitors Of Beta-Lactamase, Roberto Pestana-Nobles, Yani Aranguren-Diaz, Elwi Machado-Sierra, Juvenal Yosa, Nataly J. Galan-Freyle,, Laura X. Sepulveda-Montano, Daniel G. Kuroda, Leonardo C. Pacheco-Londono
Faculty Publications
Bacterial resistance is responsible for a wide variety of health problems, both in children and adults. The persistence of symptoms and infections are mainly treated with beta-lactam antibiotics. The increasing resistance to those antibiotics by bacterial pathogens generated the emergence of extended-spectrum beta-lactamases (ESBLs), an actual public health problem. This is due to rapid mutations of bacteria when exposed to antibiotics. In this case, beta-lactamases are enzymes used by bacteria to hydrolyze the beta-lactam rings present in the antibiotics. Therefore, it was necessary to explore novel molecules as potential beta-lactamases inhibitors to find antibacterial compounds against infection caused by ESBLs. …
A Survey Of Blind Modulation Classification Techniques For Ofdm Signals, Anand Kumar, Sudhan Majhi, Guan Gui, Hsiao-Chun Wu, Chau Yuen
A Survey Of Blind Modulation Classification Techniques For Ofdm Signals, Anand Kumar, Sudhan Majhi, Guan Gui, Hsiao-Chun Wu, Chau Yuen
Faculty Publications
Blind modulation classification (MC) is an integral part of designing an adaptive or intelligent transceiver for future wireless communications. Blind MC has several applications in the adaptive and automated systems of sixth generation (6G) communications to improve spectral efficiency and power efficiency, and reduce latency. It will become a integral part of intelligent software-defined radios (SDR) for future communication. In this paper, we provide various MC techniques for orthogonal frequency division multiplexing (OFDM) signals in a systematic way. We focus on the most widely used statistical and machine learning (ML) models and emphasize their advantages and limitations. The statistical-based blind …
A Survey Of Blind Modulation Classification Techniques For Ofdm Signals, Anand Kumar, Sudhan Majhi, Guan Gui, Hsiao-Chun Wu, Chau Yuen
A Survey Of Blind Modulation Classification Techniques For Ofdm Signals, Anand Kumar, Sudhan Majhi, Guan Gui, Hsiao-Chun Wu, Chau Yuen
Faculty Publications
Blind modulation classification (MC) is an integral part of designing an adaptive or intelligent transceiver for future wireless communications. Blind MC has several applications in the adaptive and automated systems of sixth generation (6G) communications to improve spectral efficiency and power efficiency, and reduce latency. It will become a integral part of intelligent software-defined radios (SDR) for future communication. In this paper, we provide various MC techniques for orthogonal frequency division multiplexing (OFDM) signals in a systematic way. We focus on the most widely used statistical and machine learning (ML) models and emphasize their advantages and limitations. The statistical-based blind …
Search Full Text Options Here 3 Of 3 Heat-Mitigated Design And Lorentz Force-Based Steering Of An Mri-Driven Microcatheter Toward Minimally Invasive Surgery, Martin Francis Phelan Iii, Mehmet Efe Tiryaki, Jelena Lazovic, Hunter Gilbert, Metin Sitti
Search Full Text Options Here 3 Of 3 Heat-Mitigated Design And Lorentz Force-Based Steering Of An Mri-Driven Microcatheter Toward Minimally Invasive Surgery, Martin Francis Phelan Iii, Mehmet Efe Tiryaki, Jelena Lazovic, Hunter Gilbert, Metin Sitti
Faculty Publications
Catheters integrated with microcoils for electromagnetic steering under the high, uniform magnetic field within magnetic resonance (MR) scanners (3-7 Tesla) have enabled an alternative approach for active catheter operations. Achieving larger ranges of tip motion for Lorentz force-based steering have previously been dependent on using high power coupled with active cooling, bulkier catheter designs, or introducing additional microcoil sets along the catheter. This work proposes an alternative approach using a heat-mitigated design and actuation strategy for a magnetic resonance imaging (MRI)-driven microcatheter. A quad-configuration microcoil (QCM) design is introduced, allowing miniaturization of existing MRI-driven, Lorentz force-based catheters down to 1-mm …
Functional Structure Of Biomacromolecules In Plant Biomass Using Solid-State Nmr And Dynamic Nuclear Polarization, Alex Kipchirchir Kirui
Functional Structure Of Biomacromolecules In Plant Biomass Using Solid-State Nmr And Dynamic Nuclear Polarization, Alex Kipchirchir Kirui
LSU Doctoral Dissertations
This dissertation summarizes the recent findings on complex biomacromolecules in cell wall of plants and fungi which perform important roles in cell recognition, structural build up, and energy storage. Because of the technical difficulty in characterizing these biomacromolecules, which are often polymorphic and disordered in structure, the functional structure of these biomacromolecules remains elusive. In this dissertation, I present two solid-state nuclear magnetic resonance (ssNMR) and dynamic nuclear polarization (DNP) studies of carbohydrate-rich biosystems: the energy-rich plant biomass and disease-relevant, pathogenic fungi.
First, we have investigated the secondary cell wall of plant biomass which is a carbohydrate-rich biosystem using solid …
Non-Adiabatic Excited State Molecular Dynamics Using Ehrenfest And Modulated X-Ray Absorption In Titania, Alexander Matthew Meyer
Non-Adiabatic Excited State Molecular Dynamics Using Ehrenfest And Modulated X-Ray Absorption In Titania, Alexander Matthew Meyer
LSU Doctoral Dissertations
This dissertation contains two separate sections aside from an introduction (Chapter 1): theory and methods (Chapter 2), time dependent density functional theory with Ehrenfest for excited state lifetimes in materials (Chapter 3), and simulated field modulated X-ray absorption in titania (Chapter 4).
Excited state lifetime in insulators and semiconductors can be difficult to compute using quantum chemistry due to their dense excited states. Non-radiative decay in these materials requires the use of non-adiabatic effects to dissipate energy through the means of electron-nuclear coupling such as coherent phonon generation. One method of approaching this challenge in these materials is using Ehrenfest …
Degumming Of Hemp Fibers Using Combined Microwave Energy And Deep Eutectic Solvent, Bulbul Ahmed
Degumming Of Hemp Fibers Using Combined Microwave Energy And Deep Eutectic Solvent, Bulbul Ahmed
LSU Master's Theses
Hemp is considered as one of the sustainable agricultural fiber materials. Degumming or surface modification of hemp bast is needed to produce single fibers for ensuing textile and industrial applications. The traditional degumming process necessitates a high amount of alkali, which causes detrimental environmental pollution. This study offers a new method to degum hemp fibers with reduced use of harmful alkali and precious water resources. In this work, hemp bast fibers were degummed by using combined microwave energy and deep eutectic solvent (DES). The properties of hemp fibers manufactured by this method were investigated and compared with the traditional alkali …
Electrochemical Reduction Of Hexavalent Chromium In Acetonitrile Using Glassy Carbon Electrodes, Temitope Jegede
Electrochemical Reduction Of Hexavalent Chromium In Acetonitrile Using Glassy Carbon Electrodes, Temitope Jegede
LSU Master's Theses
Hexavalent chromium Cr(VI), a form of chromium mainly generated through industrial processes, has attracted great research interests due to its severe toxicity to biological systems. A common way to remove hexavalent chromium from industrial wastes is through reduction to the non-toxic trivalent state. To this end, methods such as chemical reduction, chemical precipitation and coagulation have been used. However, these methods are limited by poor removal efficiency, formation of secondary pollutants (sludge), and are mostly applicable to aqueous solutions. Recently, electrochemical methods have been investigated for reducing hexavalent chromium, but there is a lack of data on the reduction of …
Investigation On The Structure And Dynamics Of Lithium Ion Solvation Shell By Linear And Non-Linear Infrared Spectroscopy, Xiaobing Chen
Investigation On The Structure And Dynamics Of Lithium Ion Solvation Shell By Linear And Non-Linear Infrared Spectroscopy, Xiaobing Chen
LSU Doctoral Dissertations
Lithium ion battery (LIB) is a lightweight, rechargeable and powerful battery that is used widely from mobile phones to laptops to electric cars. It has many exceptional advantages such as high energy density, high output power, relatively low self-discharge, etc. However, it has some disadvantages such as safety issues, capacity loss, and expensive manufacture. LIB is composed of three main components: electrolytes, positive and negative electrodes. Researchers have been working on improving the performance of LIB for the past decade, but only a few have focused on investigating the electrolytes at the molecular level. In addition, the molecular mechanisms behind …
Methodology Utilizing Triphosgene To Promote Cyclization Of Epoxy Ketones To Pyranoside Derivatives Via Proposed Epoxonium Ions, Isaac Camargo Dos Reis
Methodology Utilizing Triphosgene To Promote Cyclization Of Epoxy Ketones To Pyranoside Derivatives Via Proposed Epoxonium Ions, Isaac Camargo Dos Reis
LSU Master's Theses
The development of new methodologies in organic chemistry is of great importance. In fact, the discovery and creation of new reactions frequently uncovers new chemical reactivities and creates new areas of chemistry. The purpose of this thesis is to present the development of new synthetic reactions that were discovered through previous findings. Additionally, the main theme of this work relies on the intermediacy of a cationic reactive intermediate: epoxonium ions. Chapter 1 provides a brief review of bicyclic epoxonium ions and methods for their generation, as well as their utility in the synthesis of interesting heterocycles. Furthermore, Chapter 1 illustrates …
Reversing The Regioselectivity Of Indole Addition To Cyclic Unsymmetrical Oxyallyl Cations: Construction Of Diaryl Quaternary Centers, Thomas Davis Curry Ii
Reversing The Regioselectivity Of Indole Addition To Cyclic Unsymmetrical Oxyallyl Cations: Construction Of Diaryl Quaternary Centers, Thomas Davis Curry Ii
Honors Theses
No abstract provided.
Adsorption And Transport Of Colloids At Interface And In Bulk, Jingyun Lee
Adsorption And Transport Of Colloids At Interface And In Bulk, Jingyun Lee
LSU Doctoral Dissertations
Colloids are suspensions of microscopic insoluble particles dispersed in a continuum phase such as liquid or gas. Colloids are found in our everyday life from food and cosmetic industries to pharmaceutical and biomedical applications. Depending on a global minimum of the free-energy landscape, colloidal suspensions can be classified as two major classes: equilibrium or active colloidal system. This Ph.D dissertation presents strategies to engineer equilibrium self-assembled structures and out-of-equilibrium active matter using various interparticle forces.
First, we introduce the means to promote the equilibrium self-assembled structures driven by adsorption of colloids at interface. Typically, adsorption of colloids at interface is …
A Systematic Multiscale Investigation Of Nanoparticle-Assisted Co2 Enhanced Oil Recovery (Eor) Process For Shale Oil Reservoirs, Dayo A. Afekare
A Systematic Multiscale Investigation Of Nanoparticle-Assisted Co2 Enhanced Oil Recovery (Eor) Process For Shale Oil Reservoirs, Dayo A. Afekare
LSU Doctoral Dissertations
Shale oil reservoirs are prolific on the short term due to hydraulic fracturing and horizontal drilling but experience significant production decline, leading to poor ultimate recovery and leaving billions of barrels of oil buried in the ground. In this study, a systematic multi-scale investigation of an enhanced oil recovery (EOR) process using relatively inexpensive silicon dioxide nanoparticles and carbon dioxide for shale oil reservoirs was conducted. Using the Tuscaloosa Marine Shale (TMS) as a case study, aqueous dispersions of nanosilica in conjunction with CO2 were investigated at nano-to-core scales. At the nanoscale, atomic force microscope was used to investigate …
Investigations Of The Structure And Protein-Protein Interactions Of Chlamydia Trachomatis Scc4, Thilini Oshadhi Senarath Ukwaththage
Investigations Of The Structure And Protein-Protein Interactions Of Chlamydia Trachomatis Scc4, Thilini Oshadhi Senarath Ukwaththage
LSU Doctoral Dissertations
Chlamydia trachomatis (CT) is the most common, sexually transmitted bacterial disease (STD) in the world. In the developmental cycle of CT, specific chlamydia chaperone 4 (Scc4) is a unique protein with essential and multiple roles. Hence, Scc4 is significant as a virulence target for therapeutic approaches to treat chlamydial infections. A novel approach was discovered to purify tag free Scc4 by utilizing a 6X-histidine-tag on Scc1 in the co-expressed Scc4:Scc1 complex by capturing the complex on nickel-charged immobilized metal affinity chromatography resin, followed by dissociation of Scc4 with sarkosyl. Using triple resonance NMR experiments, backbone and sidechain resonances …