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Full-Text Articles in Physical Sciences and Mathematics
The Path To Preservation: Using Proteomics To Decipher The Fate Of Diatom Proteins During Microbial Degradation, Brook L. Nunn, Ying S. Ting, Lars Malmström, Yihsuan S. Tsai, Angela Aquier, David R. Goodlett, H. Rodger Harvey
The Path To Preservation: Using Proteomics To Decipher The Fate Of Diatom Proteins During Microbial Degradation, Brook L. Nunn, Ying S. Ting, Lars Malmström, Yihsuan S. Tsai, Angela Aquier, David R. Goodlett, H. Rodger Harvey
OES Faculty Publications
We drew upon recent advances in tandem mass spectrometry-based proteomic analyses in order to examine the proteins that remain after a diatom bloom enters the stationary phase, precipitates out of the photic zone, and is subjected to microbial degradation over a 23-d period within a controlled laboratory environment. Proteins were identified from tandem mass spectra searched against three different protein databases in order to track proteins from Thalassiosira pseudonana and any potential bacterial contributions. A rapid loss of diatom protein was observed over the incubation period; 75% of the proteins initially identified were not detected after 72 h of exposure …
Biochemical Composition Of Particles And Dissolved Organic Matter Slong An Estuarine Gradient: Sources And Implications For Dom Reactivity, Antonio Mannino, H. Rodger Harvey
Biochemical Composition Of Particles And Dissolved Organic Matter Slong An Estuarine Gradient: Sources And Implications For Dom Reactivity, Antonio Mannino, H. Rodger Harvey
OES Faculty Publications
The chemical composition of high molecular weight dissolved organic matter (DOM) and particulate organic matter (POM) was examined along the salinity gradient of the Delaware Estuary. DOM was collected and fractionated by tangential-flow ultrafiltration into 1-30 kDa (HDOM; high molecular weight) and 30 kDa to 0.2 μm (VHDOM; very high molecular weight) and compared to particles collected in parallel. Polysaccharides comprised 12-43% of particulate organic carbon (POC), 30-56% of VHDOM carbon, and 7.5-19% of HDOM carbon. Hydrolyzable amino acids comprised 17-38% of POC, 5.4-12% of VHDOM carbon, and 1.5-4.2% of HDOM carbon. Only 7-43% of dissolved organic nitrogen in VHDOM …
Regulation Of Algal Blooms In Antarctic Shelf Waters By The Release Of Iron From Melting Sea Ice, Peter N. Sedwick, Giacomo R. Ditullio
Regulation Of Algal Blooms In Antarctic Shelf Waters By The Release Of Iron From Melting Sea Ice, Peter N. Sedwick, Giacomo R. Ditullio
OES Faculty Publications
During summer 1995-96, we measured iron in the water column and conducted iron-enrichment bottle-incubation experiments at a station in the central Ross Sea (76°30'S, 170°40'W), first, in the presence of melting sea ice, and 17 days later, in ice-free conditions. We observed a striking temporal change in mixed-layer dissolved iron concentrations at this station, from 0.72-2.3 nM with sea ice present, to 0.16-0.17 nM in ice-free conditions. These changes were accompanied doubling of algal (diatom) biomass. Our incubation experiments suggest that conditions were iron-replete in the presence of sea ice, and iron-deficient in the absence of sea ice. We surmise …
Development And Lipid Composition Of The Harpacticoid Copepod Nitocra Spinipes Reared On Different Diets, Gregory M. Weiss, George B. Mcmanus, H. Rodger Harvey
Development And Lipid Composition Of The Harpacticoid Copepod Nitocra Spinipes Reared On Different Diets, Gregory M. Weiss, George B. Mcmanus, H. Rodger Harvey
OES Faculty Publications
We reared the harpacticoid copepod Nitocra spinipes on diets of bacteria, a diatom, or a macroalga, evaluating survivorship and growth in short-term (≤ 1 generation) experiments. Lipid content of the copepods and their diets was measured and used as an index of nutrition. Although growth, survivorship and lipid content of N. spinipes were significantly greater when fed the diatom, which had the highest lipid content of the 3 diets, the copepod was able to develop from egg to adult when fed a lipid- poor bacterial diet. Furthermore, this species was able to go through developmental molts without the addition of …
Observations Of The Phytoplankton Standing Crop At The Shelf Margin Of The Mid Atlantic Bight, Bruce B. Wagoner, Harold G. Marshall
Observations Of The Phytoplankton Standing Crop At The Shelf Margin Of The Mid Atlantic Bight, Bruce B. Wagoner, Harold G. Marshall
Biological Sciences Faculty Publications
A comparison of the total percentage cell abundance and cell biovolume relationships of major phytoplankton categories was made between two station sets across the shelf margin. Diatom values for abundance and biovolume were greater at oceanic stations compared to the outer shelf stations, with dinoflagellates having the reverse pattern. The composite contributions to biovolume and abundance in the standing crop from other phytoplankton categories were greater over the outer shelf than beyond the shelf margin. The major source of biovolume (biomass) from the outer shelf and these oceanic stations came from the diatoms and dinoflagellates, with an average mean of …