Veterinary Physiology Commons^™

Kisspeptin Receptor Agonist (Ftm080) Increased Plasma Concentrations Of Luteinizing Hormone In Anestrous Ewes, Brian K. Whitlock, Kelly A. Chameroy, Rebecca R. Payton, Shinya Oishi, Nobutaka Fujii, Joseph A. Daniel

Brian K Whitlock, PhD, DVM, DACT

Kisspeptin (KP) and the KP receptor (Kiss1r) are integral to central regulation of the gonadotropic-axis. The demonstration that intravenous infusion of KP can stimulate gonadotropin secretion and ovulation in seasonally anestrous female sheep offers a means of manipulating the reproductive axis. However, KP may be of limited clinical use because of the short circulating half-life. Kiss1r agonists with increased half-life and similar efficacy to KP in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test and compare the effects of …

The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii

Animal Science Publications and Other Works

Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.

The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii

Brian K Whitlock, PhD, DVM, DACT

Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.

Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn

Brian K Whitlock, PhD, DVM, DACT

Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …

Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …

Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, Joseph A. Daniel, A B. Milam, M E. Gafnea, Brian K. Whitlock, D H. Keisler

Animal Science Publications and Other Works

Previous research has demonstrated circulating concentrations of leptin increase in ewes during mid pregnancy then decline in late pregnancy and early lactation. The objective of this study was to more narrowly define the timing of changes in circulating concentrations of leptin with pregnancy in ewes. Katahdin ewes (n= 19) located at latitude 34.275 and longitude -85.183 (Mount Berry, GA) were utilized. Blood samples were collected weekly via jugular veinpuncture beginning immediately before ram exposure on September 23 and continuing until 4 weeks post-lambing. Ewes were exposed to a ram fitted with a marking harness for a 63 day breeding season. …

Changes In Plasma Concentrations Of Growth Hormone And Luteinizing Hormone In Ewes Following Central And Peripheral Treatment With Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

Kisspeptin (KP), a neuroendocrine regulator of gonadotropin releasing hormone, has been hypothesized as an integrator of nutrition and hormones critical to metabolism and regulation of reproduction. Recent evidence suggests growth hormone (GH) secretion may be influenced by KP. The objective of this study was to determine if the GH stimulatory effect of KP is due to actions on the hypothalamus or anterior pituitary gland in ewes. Adult ovariectomized ewes (n=8) were fitted with intracerebroventricular (ICV) cannula to facilitate central administration of experimental treatments. Ewes received one of eight treatments [four intravenously (IV) and four ICV]. Peripheral treatments [0 (Veh), 100, …

Changes In Plasma Concentrations Of Growth Hormone And Luteinizing Hormone In Ewes Following Central And Peripheral Treatment With Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele, James L. Sartin

Brian K Whitlock, PhD, DVM, DACT

Kisspeptin (KP), a neuroendocrine regulator of gonadotropin releasing hormone, has been hypothesized as an integrator of nutrition and hormones critical to metabolism and regulation of reproduction. Recent evidence suggests growth hormone (GH) secretion may be influenced by KP. The objective of this study was to determine if the GH stimulatory effect of KP is due to actions on the hypothalamus or anterior pituitary gland in ewes. Adult ovariectomized ewes (n=8) were fitted with intracerebroventricular (ICV) cannula to facilitate central administration of experimental treatments. Ewes received one of eight treatments [four intravenously (IV) and four ICV]. Peripheral treatments [0 (Veh), 100, …

Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, Joseph A. Daniel, A B. Milam, M E. Gafnea, Brian K. Whitlock, D H. Keisler

Brian K Whitlock, PhD, DVM, DACT

Previous research has demonstrated circulating concentrations of leptin increase in ewes during mid pregnancy then decline in late pregnancy and early lactation. The objective of this study was to more narrowly define the timing of changes in circulating concentrations of leptin with pregnancy in ewes. Katahdin ewes (n= 19) located at latitude 34.275 and longitude -85.183 (Mount Berry, GA) were utilized. Blood samples were collected weekly via jugular veinpuncture beginning immediately before ram exposure on September 23 and continuing until 4 weeks post-lambing. Ewes were exposed to a ram fitted with a marking harness for a 63 day breeding season. …

Pregnancy Diagnosis Using Ultrasound, M A C Johns

Journal of the Department of Agriculture, Western Australia, Series 4

Few Western Australian ewe flocks are diagnosed for pregnancy despite the precence of high numbers of barren ewes. Surveys of farm flocks in the early 1970s recorded that between 14 and 29 per cent of ewes failed to lamb each year.

Techniques for diagnosing pregnant ewes have been available for many years, but ultrasonic devices and computer technology have made it possible for skilled operators to determine whether ewes are pregnant and more importantly, whether they have one, two or more foetuses.

Once the number of pregnant ewes is known farmers can use labour more efficiently. They can minimise the …

Reproductive Wastage In Sheep, R W. Kelly

Journal of the Department of Agriculture, Western Australia, Series 4

The average lambing performance of Western Australia's ewe flock fluctuates between 60 and 70 per cent. Although this level of performance and variation between years is important for the sheep industry, individual farmers are more concerned about performance of their own flocks.

Figure shows the range in lambing performances that exist between farms in this State in 1983-84. The seriously poor performance of many flocks is apparentm - 28 percent of farms had less than 60 per cent lambing. By contrast, 24 farms had performances better than 100 per cent. The reasons for the large differences between farms ( and …

Ovulation Rate Of Ewes : Role Of Energy And Protein, E Teleni, J. B. Rowe

Journal of the Department of Agriculture, Western Australia, Series 4

Western Australia's sheep farmers are familiar with the low ovulation rate of Merino ewes and how this limits the lambing performance of ewe flocks.

One way in which ovulation rate and therefore lambing percentage may be increased is to feed seed of sweet lupin (Lupinus augustifolius) to ewes at mating. However, Department of Agriculture research has found that these increases do not show up consistently, and that there is considerable variability between farms.

If improved nutrition is to be a useful way to increasing ovulation rate, the mechanism by which nutrition affects ovulation rate must first be understood. This article …

Protecting Off-Shears Sheep In Adverse Weather, T M. Ellis

Journal of the Department of Agriculture, Western Australia, Series 4

In January 2982 in the Narrogin-Darkan area of Western Australia, about 14,500 sheep up to 40 days off-shears died after a 48-hour period of heavy soaking rain that followed a thunderstorm three days previously. An estimated 100,000 sheep died throughout the South-West at the time.

P. G. Buckman - at the time Department of Agriculture District Veterinary Officer, Narrogin - surveyed 17 properties in the area. He found that losses in unshedded sheep 0 to 40 days off-shears ranged from 0 to 84 per cent of sheep on the property with an overall average of 27.9 per cent. Rainfall in …

Diagnosing Pregnancy In Sheep : The 'Scanopreg', R H. Wroth, M. J. Mccallum

Journal of the Department of Agriculture, Western Australia, Series 4

The "Scanopreg", an ultrasonic machine for diagnosing pregnancy in sheep, is effective as early as eight to nine weeks of pregnancy, and could therefore be a useful management tool.

Sheep Fertility In Western Australia, R J. Lightfoot

Journal of the Department of Agriculture, Western Australia, Series 4

Why do one-quarter of the ewes that are joined in W.A. fail to lamb? Sheep and Wool Adviser Dr. R. J. Lightfoot analyses the eight stages in sheep reproduction, explaining the causes and possible prevention of infertility at each stage.

Pasture Legume Varieties And Ewe Fertility, T Marshall, H. E. Fels, H. G. Neil, R. C. Rossiter

Journal of the Department of Agriculture, Western Australia, Series 4

ALTHOUGH it was first thought that all varieties of subterranean clover would cause ewe infertility, research in the last 10 to 15 years has shown that varieties differ in potency.

The relative level of potency has also been shown to remain consistent for any one variety under normal conditions.

Maintaining Ewe Bodyweight For A Late Mating, T Marshall

Journal of the Department of Agriculture, Western Australia, Series 4

DEPARTMENT OF AGRICULTURE experiments have indicated that the bodyweight of ewes at mating can have a large bearing on lambing performance.

It appears that for every 10 lb. increase in pre-mating bodyweight, an increase of some 3 to 6 per cent, lambs born could be expected, depending on the lambing time.

Later Mating Improves Lambing Results, R J. Suiter

Journal of the Department of Agriculture, Western Australia, Series 4

Ovulation studies in Western Australia have indicated that increased lambing percentages in crossbred and Merino ewes could be expected from mating in February-April rather than December- January.

Such increases have been demonstrated in time of lambing trials with crossbred ewes, but had not been seen in Merino flocks until recently.

This report summarises the results of time of lambing trials with Merinos carried out at the Merredin and Wongan Hills Research Stations between 1963 and 1969.

High Pre-Mating Liveweights Improve The Lambing Performance Of Merino Ewes, R J. Suiter

Journal of the Department of Agriculture, Western Australia, Series 4

Analysis of lambing trials with Merino ewes has shown a highly significant association between pre-mating liveweight and lambing performance.

With May-June lambing ewes there was a 2.9 per cent, lambing increase for every extra 10 lb. liveweight; for July-August- September lambing ewes there was a 6.9 per cent, increase for every 10 lb. liveweight increase.

With the May-June lambing there was a critical weight below which twinning was negligible.

Causes Of Low Lambing Percentages, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

West Australian Merino flocks average about 70 per cent, lambs marked each year. Reasons for this low percentage are that as many as 30 per cent, of ewes do not lamb, twinning rates are low and from 10 to 25 per cent, of lambs born die.

Among the research projects by the Department of Agriculture's Sheep and Wool Branch have been a number directed at the various stages of reproduction in the ewe. These have given some important leads on the causes of low lambing percentages.

If you have marked less than 60 per cent, lambs in the past several …

Reproductive Wastage And The Sheep Shortage In Western Australia, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

WESTERN AUSTRALIA has an acute shortage of sheep.

It is estimated that about seven million extra sheep are needed to stock existing pastures fully; meanwhile the carrying capacity of the State's pastures is increasing at the rate of three million sheep a year.

Weaning And Weaner Management, R J. Lightfoot

Journal of the Department of Agriculture, Western Australia, Series 4

MOST of Western Australia's Merino flock owners wean their lambs in the months of August, September and October.

Too often this is done indiscriminately to fit in with other operations, or lambs are left on their mothers for long: periods until the ewes are remated.