Nanotechnology Commons^™

Binding Interactions Of Biologically Relevant Molecules Studied Using Surface-Modified And Nanostructured Surfaces, Palak Sondhi

Dissertations

This research focuses on the field of surface nanobioscience, wherein different nanosurfaces that will be used as working electrodes in the electrochemical cell are manufactured and surface modified to understand the critical binding interactions between biologically significant molecules like proteins, carbohydrates, small drug molecules, and glycoproteins. This research is essential if we are to determine whether a synthetic molecule can serve as a therapeutic candidate or diagnose a disease in its early stages. In order to fully understand the binding interactions, the study begins with defining some of the fundamental concepts, principles, and analytical tools for biosensing.

Afterwards, we addressed …

Liposome Synthesis And Evaluation In The Hek-293 Cell Line, Christine Skibinski

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Liposomes were synthesized using the thin film method. A lyophilized power of egg-derived phosphatidylcholine, stearylamine, and cholesterol were added to ethanol and dried under argon to form a lipid cake. The lipid cake was rehydrated with dPBS and sonicated at 60°C forming a heterogenous batch of liposomes. Our results revealed the average size of the liposomes, determined by Dynamic Light Scattering, was approximately 223.1nm, while demonstrating a weakly positive zeta-potential of 1.9± 8.07mv. Next, we tested the antitumor action of the liposomes in the HEK-293 cell line via an MTT assay. We observed that the liposomes were able to inhibit …

Development Of Targeted Drug Delivery System To Improve Immunotherapy In Pancreatic Cancer, Poornima Devi Shaji, Ana Martinez Bulnes, Nirnoy Dan, Subhash C. Chauhan, Sheema Khan, Murali M. Yallapu

Research Colloquium

Introduction: About 95% of tumor arises from epithelial cell lining ducts known to be pancreatic ductal adenocarcinomas, with less than 5-7% survival rate. Unfortunately, little progress has been seen in the outcomes of patients with PDAC as tumor develops high desmoplasia and chemo-resistance to chemotherapeutic drugs, such as gemcitabine (Gem). Immunotherapy has shown promising results in other cancers but limited response in pancreatic cancer due to desmoplasia and fibrotic tumor microenvironment. A recently identified mucin, MUC13 is aberrantly expressed in pancreatic tumors but not in normal pancreas. Due to its high membrane expression, MUC13 may serve as an excellent target …

Novel Nanoparticle Formulation Of Sabizabulin (Veru-111) For Pancreatic Cancer Treatment, Vivek K. Kashyap, Godwin P. Darkwah, Neeraj Chauhan, Prashanth Kb Nagesh, Anupam Dhasmana, Swati Dhasmana, Qinghui Wang, Duane D. Miller, Wei Li, Bilal B. B. Hafeez, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Background: Pancreatic cancer (PanCa) is one of the leading causes of cancer-related mortality in the United States due to very limited therapeutic options. Thus, developing novel therapeutic strategies will help for the management of this disease. We recently identified VERU-111, a novel synthetic molecule which showed potent anti-cancer effect against PanCa via targeting clinically important βIII and βIV tubulin isoforms. In this study, we synthesized and characterized its novel nanoformulation (MNP-VERU) and evaluated its therapeutic effects in vitro and xenograft mouse model.

Methods: MNPs were prepared by chemical precipitation method and loaded with VERU-111 using diffusion method. This formulation was …

A Natural Near-Infrared Fluorescent Probe For Cancer Cell Imaging, Benilde E. Adriano, Nycol M. Cotto, Neeraj Chauhan, Meena Jaggi, Subhash C. Chauhan, Murali M. Yallapu

Research Symposium

Background: Rio Grande Valley (RGV) suffers from a high prevalence of certain cancers and lack the resources for accurate early diagnosis. Near-infrared (NIR) fluorescence-based imaging is a noteworthy and safer strategy for cancer detection compared to radiological imaging. There are several NIR dyes including indocyanine green (ICG) and its analogues that allow high-resolution and deep tissue imaging. However, these dyes possess some drawbacks, namely photo instability, toxicity, poor water solubility, and short half-lives. Chlorophyll (Chl) is a natural dietary and biocompatible NIR fluorescent substance which has the potential to serve as a cancer NIR imaging candidate. Hence, we aim to …

Development, Optimization, And In Vitro/In Vivo Evaluation Of Azelaic Acid Transethosomal Gel For Antidermatophyte Activity, Ali Nasr, Noha Badawi, Yasmine Tartor, Nader Sobhy, Ali Nasr

Pharmacy

Treatment of dermatophytosis is quite challenging. This work aims to investigate the antidermatophyte action of Azelaic acid (AzA) and evaluate its efficacy upon entrapment into transethosomes (TEs) and incorporation into a gel to enhance its application. Optimization of formulation variables of TEs was carried out after preparation using the thin film hydration technique. The antidermatophyte activity of AzA-TEs was first evaluated in vitro. In addition, two guinea pig infection models with Trichophyton (T.) mentagrophytes and Microsporum (M.) canis were established for the in vivo assessment. The optimized formula showed a mean particle size of 219.8 ± 4.7 nm and a …

Leveraging Bio-Inspired Molecules For Cancer Theranostics, Douglas S. Macpherson

Dissertations, Theses, and Capstone Projects

A variety of molecules can be radiolabeled and delivered to a cancer site for the purposes of diagnostics and therapy. Among the most promising of tumor targeting molecules are peptides and antibodies. These bio-inspired molecules can be designed and synthesized to target and respond to cancer cells based on the properties of those cells. Matrix metalloproteinase (MMP) enzymes are over-expressed by some metastatic cancers, in which they are responsible for the degradation and remodeling of the extracellular matrix. In recent years, MMPs have emerged as promising targets for enzyme-responsive diagnostic probes because oligopeptides can be designed to be selectively hydrolyzed …

Cosemiprime Ring, Muntaha Abdul- Razaq Hasan

Al-Qadisiyah Journal of Pure Science

In this paper ,we introduce a new type of rings,namely cosemiprime ringas a generalization of semiprime ring,where a ring C is said to be cosemiprime ring if I= for each ideal I of C .Some properties of this type of rings are obtained . Also,we determin the relationship among types of ideals in this ring.

Review Of Key Derivation Functions In Cryptographic Systems, Hasan Kadhim A. Alsuwaiedi, Abdul Monem S. Rahma

Al-Qadisiyah Journal of Pure Science

Because of its significance in the formation of the core of encryption/decryption algorithms, the key derivation function (KDF) or pseudorandom number generator (PRNG) plays a major part in the cryptographic system. Random numbers are required for the usage of encryption and decryption techniques in a variety of network security applications. In this research we Focus on key generation functions, which are an important aspect of symmetric and asymmetric algorithms. Disgusting the background of PRNG basics and attempting to characterize and evaluate several publications that offer proposals for key generators.

The Refractive Index Of Photonic Crystal Fibers As A Function Of Some Parameters And Temperature, Mohammed Salim Jasim, Nadia Al-Aboody

Al-Qadisiyah Journal of Pure Science

Photonic crystal fibers in the late period occupied a wide range of studies and research because of the ease of dealing with them in terms of design and installation, as there is a group of parameters used in them that can affect the refractive index of the pulse propagation through it, including the diameter of the air holes, the distance between the air holes and their number, as this study showed in addition to the aforementioned parameters The effect of temperature on the refractive index was also studied. It has been observed that with an increase in the diameter of …

Common Fixed Point Theorems By Using Two Mappings In B-Rectangular Metric Space, Qasim K. Kadhim

Al-Qadisiyah Journal of Pure Science

In this paper, the aim to obtain the existence and uniqueness of some common fixed point theorem on b-rectangular metric space by using new conditions including rational, maximum, minimum and combination for two mappings.

Environmental And Diagnostic Study Of Some Parasites In River Diwaniyah-Fish Of Al Xanthopterus, Abtisam Jasim Abbas

Al-Qadisiyah Journal of Pure Science

The current study was concerned with investigating parasitic infections in Barbus Xanthopterus fish in Al-Diwaniyah River, for the period (July 2020-December 2020). (218) fish were collected from three different locations along the Al-Diwaniyah River. The results of the laboratory examination indicated that the fish were infected with the parasite Chilodonella Cyprinid and Eimiria sinenis. The current study recorded changes in the physical and chemical properties of Al-Diwaniyah River water, as the water temperature values, measured turbidity values, pH, electrical conductivity, hardness and TDS recorded a clear variation during the months of the study. The results of the statistical analysis indicated …

Some Properties Of Almost Periodic Points In A Random Dynamical System, Qays Arif Wali, Ihsan Jabbar

Al-Qadisiyah Journal of Pure Science

No abstract provided.

Preparation And Study Of Tin And Gallium Thin Films On Silicon Substrates, Maher Nadher Abdullah

Al-Qadisiyah Journal of Pure Science

In this research, the ideal procedures were obtained for growth epitaxial layers of tin and gallium solutions which are solid solutions, and the two solutions of the molten material were dissolved on a silicon substrate. The results were to obtain the lowest dislocation density of thin films. The relationship between the dislocation densities of thin films and the thickness of the thin film was an exponential relationship between its variable values. Thin films with a compound containing a smooth structure varied in order were obtained by smoothly changing the lattice parameters of the gradient gap solid solution, and an ideal …

Correlation And Comparison Of Some Trace Elements And Their Related Proteins In Type 2 Diabetes Mellitus In Kalars City/ Kurdistan-Iraq, Hero Helal Muhammed Saed, Zeyan Abdullah Ali

Al-Qadisiyah Journal of Pure Science

No abstract provided.

Antibiotic Susceptibility Of Proteus Mirabillis That Isolates Of Diabetic Foot Ulcers In Al- Diwaniyah Hospital, Aseel Hussein Jaber, Suaad A. Fazaa Almiyah

Al-Qadisiyah Journal of Pure Science

The current study have been included 250 samples diabetic foot ulcer patients attending in Al-Diwaniyah Teaching Hospital and private clinics at a period of study from beginning October 2021 to February 2022, Isolates were identified by morphological form on blood agar and macConkey agar, traditional biochemical tests and then confirmed by Vitek 2 system. The result of bacterial culture have been recorded that only 80 samples had positive result to P. mirabilis formed (32%) of this result. Prevalence of P. mirabilis isolates in male more than female (34.78%) and (28.57%) respectively, after then were determination of antibiotics susceptibility pattern of …

Supra-Approximation Spaces Using Combined Edges Systems, Hussein R. Jaffer, Khalid Sh. Al’Dzhabri

Al-Qadisiyah Journal of Pure Science

The primary in this paper's notion, the i-space using incident edges system (resp. n-space using non-incidental edges system), is what this study is responsible for generating and investigating. Additionally, we used c-interior to define the c-lower approximations in generalized rough set theory (resp. i-interior and n-interior) Additionally, the c-upper approximations are defined using c-closure (as opposed to i-closure and n-closure), and some of its characteristics are studied.

Study Of Transitive Random Dynamical Systems, Salam T. Abbas, Ihsan J. Kadhim

Al-Qadisiyah Journal of Pure Science

No abstract provided.

Candida Spp. Associated With Covid-19 And Its Susceptibility To Some Antifungals, Mohammed Mudhafar Alkhuzaie, Neeran Obied Jasim

Al-Qadisiyah Journal of Pure Science

The aim of this study was to conduct a survey of the Candida species associated with COVID-19 viral infection in 150 patients who were admitted to the intensive care unit (ICU) in Al-Diwaniyah Teaching Hospital in Al-Diwaniyah City, Iraq, for a five-month period from October 2021 to February 2022. The results indicated the dominance of Candida spp. over the rest of the isolated fungal species, with 97 isolates (64.66%). C. albicans was shown to be the most abundant species with a percentage of 55.67 percent (P 0.05), compared to the other Candida species that were isolated (Candida tropicalis 13.4 percent, …

Fungal And Trichothecenes (Tct) Contamination Of Poultry Feed From Duhok Province, Kurdistan Of Iraq, L. F. Hussei A. A. M. Saadullah

Al-Qadisiyah Journal of Pure Science

Fungi are capable of reducing the nutritional value of food for human and animals and producing many mycotoxins. Mycotoxins-contaminated animal feed has adverse effects on productivity and animal health. As well, mycotoxins may be carried over into meat and eggs when poultry are fed with contaminated feed. Data on the occurrence of T-2 and HT-2 toxins in poultry feed from Kurdistan region of Iraq are very limited. T-2 toxin is the most toxic type A trichothecene mycotoxin. It is the secondary metabolite of the Fusarium fungi, and is common in grains and animal feed. Toxic effects have been shown both …

Omitted - Value Transformation For A Complex Differential Equations, Shatha S. Alhily, Fatima Ah. Mazloum

Al-Qadisiyah Journal of Pure Science

No abstract provided.

Comparative Physiology Study Of Side Effect Between Xenical And Lipo-6 Supplements Which Treated Obese Rabbets, Hayder Ghazib Abdulshaheed

Al-Qadisiyah Journal of Pure Science

Obesity is rapidly becoming a health problem, and it is considered a metabolic disease of epidemic proportions. Obesity is also associated with many comorbidities such as hyperlipidemia, fatty liver, atherosclerosis, cancer. Use of over-the-counter weight-loss supplements has become more common in the world; Xenical and Lipo-6 are two examples of nutritional supplements that do not pass safety research before being sold to the consumer. The ability of Xenical and lipo-6 supplements on DNA fragmentation in human lymphocytes was studied, where these cells represent important defense line of the body. On other hand, thirty white rabbits Iraqis in this experiment, weighing …

Steganography Using Syntactic Method, Saad Nasser Al.Azzam, Fahad Ali Al-Qarni

Al-Qadisiyah Journal of Pure Science

Information security, which is becoming more crucial as information sharing plays an increasingly significant part in the day-to-day activities of today's people, is one of the methods that may be used for information security. For the sake of information security, we present a method to linguistic steganography that is based on syntactic banks.

While the unprocessed input a phrase structure of the sentence is produced by parsing the sentence using the Stanford parser, which can create a phrase structure of the sentence.

They create the grammatical structure of the sentence. The input secret message is encoded using Shannon-Fano coding in …

Water Quality Evaluation Of The Main Drain In Dhi-Qar Governorate, South Iraq, Ali H. Nomas, Ayser Al-Shamma

Al-Qadisiyah Journal of Pure Science

The Main Drain is one of the largest irrigation projects which is used to collect drainage water from the lands extended on both sides of the water channel and diverts it to Shatt Al-Arab and from there to the Arabian Gulf. The Main Drain passed in Dhi-Qar Governorate to the northeast of Nasiriyah oil field in the Kati’a area, which is about 38 km from the center of the city of Nasiriyah. The objective of the present research is to evaluate the water quality, and its temporal change of the Main Drain, and if there is an impact of the …

Proposing An Rna Interference (Rnai)-Based Treatment For Human Immunodeficiency Virus (Hiv) By Analyzing The Post-Transcriptional Gene Targeting Of Sars-Cov-2, Hepatitis C Virus, And A549 Lung Cancer Cells, Arjun Jagdeesh

Undergraduate Research Posters

Human Immunodeficiency Virus (HIV) is a retrovirus that infects CD4+ T cell lymphocytes in humans, leading to the development of Acquired Immunodeficiency Syndrome (AIDS) if left untreated. While current treatment methods, including antiretroviral combination treatments, effectively limit HIV replication, HIV can evade these treatments due to its high mutation rate. Long-term antiretroviral treatment can also be toxic to patients, meaning patients would benefit from a new mechanism of HIV treatment. RNA interference (RNAi) is an antiviral pathway found in mammals, plants, and insects that involves a small-interfering RNA that is incorporated into a protein complex called the RNA-induced Silencing Complex …

Frontiers In The Self-Assembly Of Charged Macromolecules, Khatcher O. Margossian

Doctoral Dissertations

The self-assembly of charged macromolecules forms the basis of all life on earth. From the synthesis and replication of nucleic acids, to the association of DNA to chromatin, to the targeting of RNA to various cellular compartments, to the astonishingly consistent folding of proteins, all life depends on the physics of the organization and dynamics of charged polymers. In this dissertation, I address several of the newest challenges in the assembly of these types of materials. First, I describe the exciting new physics of the complexation between polyzwitterions and polyelectrolytes. These materials open new questions and possibilities within the context …

Controlling Myosin’S Function Via Interactions Between The Substrate And The Active Site, Mike K. Woodward

Doctoral Dissertations

Molecular motors, such as myosin, have evolved to transduce chemical energy from ATP into mechanical work to drive essential cellular processes, from muscle contraction to vesicular transport. Dysfunction in these motors is a root cause of many pathologies necessitating the application of intrinsic control over molecular motor function. We hypothesized that altering the myosin’s energy substrate via minor positional changes to the triphosphate portion of the molecule will allow us to control the protein and affect its in vitro function. We utilized positional isomers of a synthetic non-nucleoside triphosphate, azobenzene triphosphate, and assessed whether myosin’s force- and motion-generating capacity could …

Producing And Measuring Oscillatory Shear In A Novel Microfluidic Chip, Sanaz Lordfard, Daniel Lorusso, Tamie L. Poepping, Hristo N Nikolov, Kayla Soon, Stephen Sims, Jeffrey Dixon, David Holdsworth

Undergraduate Student Research Internships Conference

Purpose: To demonstrate the effectiveness of a novel microfluidic device mimicking oscillatory blood flow, allowing cell biologists to examine how endothelial cells respond to a range of oscillatory shear stress levels.

Methods: The microfluidic chip consists of a circular-shaped reservoir, leading to a rectangular channel that is examined under a microscope. The plunger is connected to a speaker system and oscilloscope, allowing the plunger to apply a range of frequencies (5-60Hz) and voltages (5-10 V, leading to a variety in oscillation amplitudes) to the reservoir region. 1.1 um fluorescent particles diluted in distilled water were used for tracking. Processing was …

Synthesis And Characterization Of Cr-Znga2o4 And Eu-Ca3(Po4)2 Composite, Benjamin Hulme

Undergraduate Student Research Internships Conference

No abstract provided.

Isolation And Characterization Of Α-Hcd7 Scfv Expressing Exosomes And Synthesis Of Hybrid Exosome-Liposome For Analyses, Sahil Patel

Biotechnology Theses

The goal of this thesis project is to isolate and characterize exosomes that express anti-human CD7 (α-hCD7) single chain variable fragment (scFv), which will be further utilized for T celltargeted gene editing. To reach this goal, exosomes from HEK 293 cells and Jurkat cells were isolated and characterized with different methodologies. To obtain α-hCD7 scFv-expressing exosomes, the HEK 293 cells were subjected to transfection of custom-plasmid pDisplay-αhCD7 scFv, and the expression of α-hCD7 scFv on the HEK 293-derived exosomes were then confirmed by immunogold staining, western blotting, and flow cytometry. To transfect 293 cells, calcium phosphate transfection was utilized, and …