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Full-Text Articles in Medical Genetics
The Role Of Long Noncoding Rnas In Ocular Angiogenesis And Vascular Oculopathy, Pranali Gandhi, Yuzhi Wang, Guigang Li, Shusheng Wang
The Role Of Long Noncoding Rnas In Ocular Angiogenesis And Vascular Oculopathy, Pranali Gandhi, Yuzhi Wang, Guigang Li, Shusheng Wang
School of Medicine Faculty Publications
Background: Long noncoding RNAs (lncRNAs) are RNA transcripts over 200 nucleotides in length that do not code for proteins. Initially considered a genomic mystery, an increasing number of lncRNAs have been shown to have vital roles in physiological and pathological conditions by regulating gene expression through diverse mechanisms depending on their subcellular localization. Dysregulated angiogenesis is responsible for various vascular oculopathies, including diabetic retinopathy, retinopathy of prematurity, age-related macular degeneration, and corneal neovascularization. While anti-VEGF treatment is available, it is not curative, and long-term outcomes are suboptimal, and some patients are unresponsive. Results and summary: To better understand these diseases, …
Exosomes From Adipose-Derived Stem Cells Alleviate Myocardial Infarction Via Microrna-31/Fih1/Hif-1Α Pathway, Dihan Zhu, Yang Wang, Miracle Thomas, Keasiah Mclaughlin, Babayewa Oguljahan, Joshua Henderson, Qinglin Yang, Y Eugene Chen, Dong Liu
Exosomes From Adipose-Derived Stem Cells Alleviate Myocardial Infarction Via Microrna-31/Fih1/Hif-1Α Pathway, Dihan Zhu, Yang Wang, Miracle Thomas, Keasiah Mclaughlin, Babayewa Oguljahan, Joshua Henderson, Qinglin Yang, Y Eugene Chen, Dong Liu
School of Graduate Studies Faculty Publications
Our previous study has revealed that exosomes from adipose-derived stem cells (ASCs) promote angiogenesis in subcutaneously transplanted gels by delivery of microRNA-31 (miR-31) which targets factor inhibiting hypoxia-inducible factor-1 (FIH1) in recipient cells. Here we hypothesized that ASC exosomes alleviate ischemic diseases through miR-31/FIH1/hypoxia-inducible factor-1α (HIF-1α) signaling pathway. Exosomes from ASCs were characterized with nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting analysis for exosomal markers. Results from immunoblotting and laser imaging of ischemic mouse hindlimb revealed that miR-31 enriched ASC exosomes inhibited FIH1 expression and enhanced the blood perfusion, respectively. These effects were impaired when using miR-31-depleted exosomes. Immunohistochemistry …