Medicine and Health Sciences Commons^™

Role Of Myd88 Protein In The Maintenance Of Immunological Self-Tolerance: Induction Of Autoimmunity By Salmonella Infection Consequent To Myd88 Deficiency, Jincy Merin Issac

Dissertations

Activation of the innate immune system is a prerequisite for the induction of adaptive immune responses to both infectious and non-infectious agents. Toll-like receptors (TLRs) are a family of proteins important for recognizing pathogen associated molecular patterns. MyD88 is an adaptor molecule whose function is critical in TLR signaling. MyD88-deficient (MyD88-/-) mice exhibit heightened susceptibility to infections, even by attenuated strains of Salmonella enterica serovar Typhimurium. Paradoxically, despite their hypersusceptibility, infected MyD88-/- mice produce elevated serum levels of anti-Salmonella antibodies regardless of the route of infection. This hypergammaglobulinemia was observed with both Th1-driven (IgG2c and IgG3) and Th2-driven (IgG1) antibody …

The Role Of Glutamate Signalling In Diabetic Neuropathy, Nadia Hussain

Dissertations

The majority of diabetics develop neuropathy, which can be debilitating, but the underlying pathophysiological mechanisms are poorly understood. Diabetic neuropathy progresses in a distal to proximal manner. Previous studies have shown that glutamate, the most common excitatory neurotransmitter, plays a role in the pathogenesis of neuropathy. The reason why the role of glutamate in nociception becomes a problem in diabetes and the mechanisms that are involved are unknown. Based on the preliminary data, the hypothesis was that glutamate pathways are likely to be involved in diabetic neuropathy particularly neuropathic pain. Pathways were investigated to look for changes that might reflect …

Hypoxic Signals In The Ischemic Myocardium: Role Of Galectin-1 And Galectin-3, Satwat Hashmi

Dissertations

Myocardial infarction is the most serious manifestation of coronary artery disease and the cause of significant levels of mortality and morbidity worldwide. Galectin-1 (GAL-1) and Galectin-3 (GAL-3) are beta galactoside binding lectins with diverse functions. Hypoxia inducible factor-1 alpha (HIF-1α) is a transcription factor mediating early and late responses to myocardial ischemia. We aim to study the direct effects of ischemia on GAL-1, GAL-3 and HIF-1α in the heart. Male C57B6/J and GAL-3 knockout mice were used for our two disease models. In the Myocardial infarction (MI) model, the left anterior descending artery of the heart is permanently ligated to …

Effects Of The Endocannabinoid Anandamide On Excitability And Calcium Signaling In Rat Ventricular Myocytes, Lina Tareq Al-Kury

Dissertations

Endogenous cannabinoids (endocannabinoids) exert a wide range of biological effects. In addition to having their well-known neurobehavioral effects, a role for the major endocannabinoid anandamide (N-arachidonoyl ethanolamide; AEA), in the cardiovascular system in various pathological conditions has been reported. The aim of this thesis is to explore the effects of AEA on contractility, Ca2+ signaling, and action potential (AP) characteristics in rat ventricular myocytes. A video edge detection system was used to measure myocyte shortening. Intracellular Ca2+ was measured in cells loaded with the fluorescent indicator fura-2 AM. AEA (1 μM) caused a significant decrease in the amplitude of electrically-evoked …

Interventions For Childhood Obesity: Evaluating Technological Applications Targeting Physical Activity Level And Diet, Jessica Dipietro

HIM 1990-2015

Overweight and obese children have increased risks for multiple preventable diseases and conditions which can impair their physiological health and significantly increases the overall cost of their healthcare. Free mobile applications and technology for weight loss, dietary tracking, and physical activity may be quite useful for monitoring nutritional intake and exercise to facilitate weight loss. If so, nurses are well positioned to recommend such tools as part of their efforts to prevent childhood obesity and help children and parents better manage childhood obesity when it is present. However, there are no guidelines that nurses can use to determine what applications …

Molecular Chaperone Tools For Use Against Neurodegenerative Diseases, Matthew Tinkham

Senior Honors Projects

A noted characteristic found in several neurodegenerative disorders, including Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease and bovine spongiform encephalopathy, is the accumulation of amyloid plaques in the brain. Amyloid plaques contain deposits of fibrillar aggregates of misfolded proteins that disrupt normal functionality in neurons. Certain variants of these misfolded proteins are self-replicating; these self-replicating amyloids are termed prions (for infectious protein). We are interested in how protein misfolding contributes to amyloid formation and how molecular chaperone proteins can change the formation of amyloid deposits. Chaperone proteins function by catalyzing the proper folding of other proteins, the refolding of misfolded proteins, …

Assessing The Relationship Between The Blood-Air Partition Coefficient And Fractional Uptake Of Inspired Halothane, Acetone, And Ethanol Vapors In The Airways Of The C57bl/6j Mouse, Joshua Baruch Baldino

Honors Scholar Theses

The following series of studies investigates the elimination and uptake trends of halothane, acetone, and ethanol vapors in the airways of C57BL/6J mice. These vapors were chosen because they span a wide range of solubilities, as indicated by their blood-air partition coefficients, and are not associated with any significant airway metabolism or reactivity with tissue substrates in vivo. Mice were exposed to a homogeneous vapor mixture containing a 1:1:1 ratio of halothane, acetone, and ethanol at relative concentrations of approximately 10 ppm. Exposure studies were performed with mice in two states, conscious and deceased, in order to provide control …

The Effect Of Repeated Mild Traumatic Brain Injury On Ventricular Volume And Microglial Activation, Lillian Rose Talbot

Honors Scholar Theses

As the leading cause of death and disability in individuals under the age of 45-years-old, Traumatic Brain Injury (TBI) is a public health crisis that demands the attention of the scientific and medical community [28]. The majority of all TBIs that occur in the United States each year are a non-deadly yet detrimental form of closed brain injury known as mild TBI (mTBI) or concussion [6]. Athletes, young people and military personnel all face a high risk of acquiring mTBI as a result of their environments. In our study we have chosen to model repeated mTBI (rmTBI) in the mouse …

Age-Related Changes In Attention During Motor Learning, James E. Gardner

Undergraduate Honors Capstone Projects

Theories of motor learning predict that humans require high levels of attention to perform new motor tasks, but little to no attention for those that are well-learned. Thus, practicing a task may decrease the amount of attention required to perform it. To test this theoretical relationship between attention and task practice, we used a physiological proxy for attention known as electrodermal activity (EDA). We hypothesized that 1) EDA (proxy for attention) would decrease over the course of training and that 2) attention would be higher overall in older adults than in younger adults when performing the same task. This second …

Biomechanical Analysis Of Gait Kinetics Resulting From Use Of A Vacuum Socket On A Transtibial Prosthesis, Maria De Lourdes Ramos Gonzalez

Honors College Theses

The technology and design of lower limb prosthetics have evolved greatly since their introduction. The current study proposed to compare the effects of a conventional pin socket attachment and a vacuum socket attachment for a transtibial amputee. Whereas traditional measurements of gait utilize force plates and camera systems, this study made use of a small tri-axial load cell located at the base of the socket to measure the forces and moments exerted during a regular gait cycle. The hypothesis tested stated that a vacuum pump socket attachment, when compared to a non-vacuum (pin) socket, will have a significant effect on …

Killing And Letting Die: The Irrelevant Distinction, Sarah Beth Shaw

Honors College Theses

The object of this essay is to explain why the distinctions made in euthanasia between killing vs. letting die and willingness to kill vs. unwillingness to kill are not relevant to real life euthanasia cases. The specific purpose of the research is to isolate the relevant factor for debate when discussing the morality of euthanasia. It begins with a brief examination of some vocabulary that is commonly used when discussing euthanasia. Following this is a quick overview of what the word euthanasia meant in the ancient Greco-Roman world compared to what it means in the modern vernacular. I use an …

Using The Theory Of Planned Behavior To Explain Physical Activity Among College Students, Ese B. Aghenta

Masters Theses & Specialist Projects

The health benefits of physical activity include improved cardiovascular health, reduced rates of diabetes and other metabolic diseases, weight maintenance and improved bone and mental health (United States Department Health & Human Services (2008). According to the American College Health Association National College Health Assessment (ACHA-NCHA, 2013), only 20.0% of college students in the United States meet this recommendation. The Theory of Planned Behavior (TPB) attempts to explain the intention to perform behaviors that are not under an individual’s complete volitional control and has been highly utilized in predicting intention and performance of physical activity (Courneya, Nigg & Estabrooks, 1998). …

Improving Running Performance Using Non-Running Tasks, Darien Lewis

Honors College

For decades researchers and runners have endeavored to improve endurance running performance. Recent evidence suggests that adding resistance training to a running program can improve running performance. Our purpose was to determine if 6wks of resistance exercise via negative eccentrically-induced work (RENEW), an exercise that increases muscle size, strength, and stiffness, could serve as an effective “non-running” modality for improving running performance. Recreational runners were assigned to a: RENEW (n=7), plyometric (PLYO, n=7), or control (CON, n=7) group. RENEW trained on an eccentric ergometer (3x/wk, 10-30min, 54-66% of peak heart rate) while the PLYO performed …

Media Framing Of Yusuf Islam’S An Other Cup: Music, Religion, And Narrative Construction, Joshua Hamburg

Honors Theses

No abstract provided.

The Role Of Faith-Based Clinics In America's Healthcare System, Parker Jennings Loy, Parker Jennings Loy

Chancellor’s Honors Program Projects

No abstract provided.

Health Care Delivery And The Hispanic Community In Knox County, Sean C. Barton

Chancellor’s Honors Program Projects

No abstract provided.

The Spread And Potential Control Of Disease Across The Domestic Cattle-Wildlife Interface, Rachel Jackson

Chancellor’s Honors Program Projects

No abstract provided.

Nicotine And What Else?: Hplc Elution Optimization For The Analysis Of Alkaloids Found In Electronic Cigarettes, Jessica L. Murray

Honors Theses

Electronic cigarettes, or e-cigarettes, have been scrutinized by the Food and Drug Administration and other public health organizations for issues related to quality control and efficacy. Erratic nicotine delivery and numerous toxic compounds in refill solutions have been reported. The ultimate goal of this research is to add to the growing amount of knowledge regarding e-cigarette ingredient content, safety, and quality. Standards of the tobacco alkaloids nicotine, anabasine, cotinine, and myosmine were prepared and quantified using high performance liquid chromatography (HPLC). Various elution schemes were tested and adjusted for optimal analyte resolution, and a final elution scheme was developed to …

Purple Urine Bag Syndrome: Regulation Of Pyrimidine Enzymes, Ashley N. Broadway

Honors Theses

Purple Urine Bag Syndrome, PUBS, is a unique condition that shows an interesting discoloration of the urine from women who have a chronic catheter. This study focused on the regulation of enzyme activities to determine the effects that may create an environment where PUBS is seen in patients with catheters. In this study, the reductive pyrimidine pathway was used to read out the bacteria Pseudomonas aeruginosa ATCC 15692. This microorganism expresses a pathway that synthesizes indoxyl from tryptophan. The bacteria strains were grown using various carbon and nitrogen sources. Concentrations of acetonitrile at 2 mM and 10 mM, concentrations of …

Epigenetic Remodeling In An Imr-32 Cell Line And Transgenic Mouse Model Of Alzheimer's Disease, Matthew S. Baker

Senior Honors Theses

The pathological features of Alzheimer’s disease (AD) have been researched and documented extensively, however the causes of these features are still unknown. The following studies sought to determine if epigenetic methylation alterations contribute to AD. Two studies were sequentially carried out, first using an IMR-32 model and then using a transgenic mouse model overexpressing beta-amyloid. A few assay and confirmation methods were carried out to determine the promoter regions in disease state models undergoing drastic change, and the genes linked to these promoter regions were analyzed to determine significant gene ontology being altered by this epigenetic modification. This data was …

Awareness, Education, And Prevention Of Chronic Kidney Disease In The Older Adult, Torrel R. Bridges

Honors Theses

Chronic kidney disease (CKD) has become a major issue in our nation. Many of our neighbors, family, and friends have concerns and a call for our attention is necessary. CKD does not have a specific target, but individuals with diseases such as diabetes mellitus, cardiovascular disease, and obesity are all at increased risk. Adults older than 65 years of age are especially at increased risk for developing CKD due to decrease in kidney function and other physiologic changes. In light of this, awareness, education, and prevention of CKD in the older adult population is important.

The number of older individuals …

Body Mass And Depression Among Mississippi Women With Polycystic Ovarian Syndrome, Laurel A. Bruce

Honors Theses

Objective

The purpose of this study is to evaluate the relationship between body mass and depression among Mississippi women with polycystic ovarian syndrome (PCOS).

Methods

Ten women participated in this study. Data was collected by online survey. The survey was distributed to eligible participants on pcoschallenge.com, a support network for women with PCOS. The survey included the Center for Epidemiologic Studies Depression (CES-D) questionnaire as well as five additional questions that asked the participants to report their weight, height, comorbidities, age, and race. The CES-D score provided a measurement for depression while the participant’s weight and height allowed the researcher …

The Comparison Of The Effectiveness Of Using A Traditional Approach To Speech Therapy To Using A Traditional Approach To Speech Therapy Plus Non-Speech Oral Motor Exercises In Children With Articulation Disorders, Alanna D. Buckley

Honors Theses

This study focuses on an area of debate in the field of Speech-Language Pathology with little prior research on the topic. The thesis considers the use of non-speech oral motor exercises in children with Articulation Disorders. Understanding the reasoning behind the use of these exercises as well as the reasons that cause the exercises to be controversial are discussed. Data is collected from four clients at the University of Southern Mississippi Speech and Hearing Clinic. All four clients have Articulation Disorders; non-speech oral motor exercises are used in therapy with two of the clients in addition to a Traditional Approach …

Does Knowledge Make A Difference? Assessing Nursing Students’ Knowledge Of Proper Hand Hygiene Techniques In Correlation With Their Progression Through Nursing School, Charika R. Carradine

Honors Theses

Proper hand hygiene techniques have been widely accepted as the most effective way for health care workers to prevent the spread of diseases. The main focus of this research was to determine differences between nursing students’ semester level and their knowledge of proper hand hygiene techniques. A convenience sample was obtained from the nursing student population. The sample population included nursing students enrolled in the second, third, and fourth semester in nursing school. Data collected was analyzed using SPSS version Twenty. A Parametric and descriptive statistics were used to measure the magnitude of relationships between variables, and describe students’ knowledge …

Solving The Obesity Problem One Bite At A Time: A Review Of Interventions, Sally Catherine Davis

Honors Theses

Resource limitations contribute to obesity in southern rural populations. An examination of published research provides evidence related to factors that lead to obesity and to related health consequences. Resource limitations in southern rural areas include a lack of access to healthy foods, a lack of safe areas to exercise or fitness equipment, and a lack of funding to promote the hiring of adequate numbers of healthcare workers to implement prevention programs and treat obesity related diseases. An investigation of obesity rates in Mississippi and Louisiana demonstrate that high rates of obesity exist. Through an exploration of published interventions in both …

Humor As A Buffer For Stress In Nurse Anesthesia Students, Kayla C. Forbis

Honors Theses

This simple, quantitative study was carried out on 18, first-year, CRNA students to determine if humor could act as a buffer for some of his or her perceived stress. Before an impending final exam, when stress levels would most likely be at their highest, the students were broken into three groups: a control group, a non-humorous group, and a humorous group. The students in the control group ranked their level of stress on a 0-10 scale without any other intervention. The other two groups watched a video clip, either humorous or non-humorous, while ranking their perceived stress on a scale …

“Birth Order’S Effect On Language Delay Detection In Young Children”, Victoria C. Souhlas

Honors Theses

In the field of Speech-Language Pathology, research has shown that the earlier a child is diagnosed with a speech and language disorder, the better the prognosis is for a favorable therapy outcome (Carroll, Bowyer-Crane, Duff, Hulme, Snowling, 2011). This researcher concentrated on how the birth order of a child can affect the prognosis of a speech and language delay. For this study, the goal was to collect quantitative data in order to view the extent of the correlation between birth order and the age of diagnosis. A sample population was found at the DuBard School of Language Disorders at the …

Auditory Versus Cursive Writing Drills In Learning New Vocabulary In 4th Grade Children, Rachel S. Tyrone

Honors Theses

Vocabulary acquisition is fundamental in order for children to succeed not only academically, but also socially. Throughout the years, many scholars have researched various techniques to aid children in learning new vocabulary. Two of the techniques to aid vocabulary can be seen by utilizing an auditory and a writing technique. A teacher will present the students with several vocabulary words multiple times throughout a regular school year. These words are vital to the success of the child. Consequently, there is a need to discover a technique that will aid the students in learning the new vocabulary words. In order to …

A Systematic Column Performance Comparison For The Confirmation Of Opioids Used In Pain Management By Lc-Ms, Derek Stallard

Undergraduate Honors Theses

In this study, three different chromatographic column chemistries (C18, Pentafluorophenyl (PFP), Hydrophilic Interaction Chromatography (HILIC)) were compared under optimal conditions to determine which stationary performed best in the separation and detection of a mixture of opioids using LC-MS. Furthermore, these stationary phases were examined in three different column technologies – traditional silica, porous shell, and porous polymer (PRP). The PRP column had the best peak shape for all 13 opioids and dominated for later-eluting compounds. In terms of column reproducibility, the Hamilton C18 column had the lowest %RSD values. The Kinetex HILIC produced the most theoretical plates and best resolution …

Soccer Participation And Youth Obesity In The United States, Lee Schober

Chancellor’s Honors Program Projects

No abstract provided.