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Genetic Polymorphism Of Cytochrome P450-1a2 (Cyp1a2) And N-Acetyltransferase-2 (Nat2) Among Emiratis, Mohammed Majed Al-Ahmad
Genetic Polymorphism Of Cytochrome P450-1a2 (Cyp1a2) And N-Acetyltransferase-2 (Nat2) Among Emiratis, Mohammed Majed Al-Ahmad
Theses
Brief introduction: There are limited studies on CYP1A2 and NAT2 polymorphisms among Emiratis. Aims: This study aims to determine CYP1A2 and NAT2 alleles and genotypes and correlate these genotypes with caffeine metabolism phenotypes among Emiratis. Methods: After obtaining informed consent, five hundred and eighty-one non-smoker subjects were given 300ml of caffeinated soft drink and were asked to provide a buccal swab and a urine sample two hours later. TaqMan Real Time PCR, PCR-RFLP, DNA sequencing were performed to determine CYP1A2 and NAT2 alleles and genotypes. Phenotyping was carried out by analysing the caffeine metabolites using HPLC analysis. Results: We found …