Medicine and Health Sciences Commons^™

Characterization Of A Novel Fungal-Specific Gene, Fug1, In Fusarium Verticillioides, John Byron Ridenour

Graduate Theses and Dissertations

Filamentous fungi are responsible for numerous plant and animal diseases. The filamentous ascomycete Fusarium verticillioides is a globally important pathogen of maize, capable of causing severe yield reductions and economic losses. Also of substantial concern is the contamination of infected kernels with fumonisins, toxic secondary metabolites linked to toxicoses in humans and livestock. The number of sequenced fungal genomes is rapidly increasing. However, functional characterization of fungal genes has not progressed at a comparable rate. In pathogenic fungi, uncharacterized genes represent a source for novel virulence factors or anti-fungal targets. Therefore, to fully understand the genetic mechanisms underlying fungal pathogenesis, …

Development Of A Heart Rate Variability Measurement System Using Embedded Electronics, Naresh Kumar Velmurugan

Graduate Theses and Dissertations

Recent advances in embedded electronics have a remarkable influence on the health care system. One of the most important applications is to monitor the health care of the patients at anytime and anyplace. In the last two decades, many researchers have focused mainly on heart rate variability (HRV) measurements. Patient's heart rate variability should be continuously monitored to help them in case of emergency. Under these circumstances, patients are required to have a HRV measuring kit for a constant observation.

The proposed project focuses on the development of a heart rate variability measurement system with the use of embedded electronics. …

Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps, Xiaofan Wang

Graduate Theses and Dissertations

The objective of this study was to evaluate the intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell line as a model to study the innate immune function of live pigs. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were evaluated using a hemocytometer and spectrophotometer for cell quantity measurements to determine growth rate and doubling time. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were 0.4016 and 0.2851 times of doubling/day respectively with a doubling time of 1.73 d and 2.43 d, respectively. Confluent IPEC-J2 monolayers were tested at five time intervals (0, 1, 2, …

The Elimination Of Listeria Monocytogenes Biofilms From Stainless Steel Deli Meat Slicer Components By The Use Of Hurdle Technologies, Alexandria Wynne Mertz

Graduate Theses and Dissertations

Listeria monocytogenes growth is of the greatest concern amongst ready-to-eat foods. The United States Department of Agriculture's Food Safety and Inspection Services determined that deli luncheon meats pose the greatest risk of contamination from L. monocytogenes Industrial meat slicers have many removable parts that are connected with sealers and gaskets, which can become worn over time. These spaces cannot be cleaned adequately, therefore are susceptible to bacterial growth. Planktonic cells form biofilms in order to protect the cell from adverse conditions, like during routine cleaning and sanitation. Once a biofilm is formed, the bacteria are much more difficult to eradicate …

Development Of A Turkey Coccidiosis Vaccine Candidate: An Attenuated Line Of The Protozoan Parasite, Eimeria Meleagrimitis, Thilakar Rathinam

Graduate Theses and Dissertations

Coccidiosis is an economically important enteric disease of poultry caused by protozoan parasites belonging to the genus Eimeria. Drugs are most commonly used to control coccidiosis. Widespread usage of anticoccidial drugs in the field has resulted in development of drug resistant strains and consequently decreased drug efficacy. An alternative control strategy using attenuated vaccines is desired. A series of experiments was designed to identify, isolate and attenuate a strain of a common and pathogenic species of Eimeria of turkeys, E. meleagrimitis.

Sensitivity of field isolates of turkey coccidia to the anticoccidials drugs amprolium, clopidol, diclazuril, and monensin was investigated. A …

Identification And The Significance Of Selective Proteins In Bile And Plasma Of Normal And Health-Compromised Chickens, Balamurugan Packialakshmi

Graduate Theses and Dissertations

During the last 50 years, animal breeding programs in commercial poultry have made significant progress in the bodyweight gain of broilers but led to several metabolic and skeletal disorders. Lameness associated with proximal femur known as femoral head separation (FHS) or femoral head necrosis (FHN) is one of the major metabolic disorders in poultry industry. In order to select for healthy chickens, markers that can distinguish between healthy and affected birds are required. Biomarkers from blood represent an ideal and rich source of markers which can be obtained using minimally invasive methods. The biomarkers were explored in an experimental model …

Ethnic And Language Matching: Effects On Hispanics' Treatment Perceptions, Carlos Adrian Ojeda Cedeno

Graduate Theses and Dissertations

Cultural adaptations to mental health treatment have been recommended to improve treatment outcomes in minorities, including Hispanics (Griner & Smith, 2006). One such adaptation includes matching the therapist to the client on culturally salient variables, such as spoken language or ethnic background. Yet, most investigations about the efficacy of matching have been correlational or have not examined language and ethnic match together (Cabassa, 2007). I investigated the effects of both ethnic and language matching on Hispanics' perceptions of psychological treatment. Participants were 100 Hispanic adults (36 men) randomly assigned to one of four conditions. In each condition, participants read a …

Enhancement Of Antimicrobial Activity Of Naturally Occuring Phenolic Compounds By Nano-Particle Mediated Delivery Against Listeria Monocytogenes, Escherichia Coli O157:H7, And Salmonella Typhimurium, Madhuram Ravichandran

Graduate Theses and Dissertations

Food-borne illnesses are of major concern in the U.S. and worldwide. Salmonella,Listeria,E. coli O157:H7 continue to be some of the major foodborne pathogens. Recurring recalls due to these pathogens demand critical antimicrobial strategies to decontaminate the food through its procession from farm to fork. In our study, we combined the effectiveness of naturally occurring phenolic compounds individually, with/ without ethylenediamine tetraacetic acid (EDTA) and/or Nisin and nanoparticle delivery to improve the antimicrobial potential of the compounds against Listeria monocytogenes (L.m.), Salmonella Typhimurium (S.T.), and Escherichia coli O157:H7 (E.c.), in brain …

Understanding Service Utilization Disparities And Depression In Latino Parents And Children: The Role Of Fatalismo, Elizabeth Anastasia

Graduate Theses and Dissertations

Research has repeatedly demonstrated a disparity between need and utilization of mental health services for Latino adults and children. The adapted Health Beliefs Model (Henshaw & Freedman-Doan, 2009) provides a useful framework for conceptualizing the roles of perceived severity and therapy expectations in the relation between demographic variables and service utilization. Cultural variations in perceptions of mental illness by Latinos may be linked with lower service utilization rates for Latino parents and children. It was speculated that fatalismo, a cultural construct similar to external locus of control, may be related to perceptions of mental illness and service utilization outcomes for …

Development And Characterization Of An Autologous Whole Cell Breast Cancer Vaccine, Samantha Leigh Kurtz

Graduate Theses and Dissertations

Approximately 40,000 women will die from breast cancer in the United States in 2014. About 90% of these deaths will be due to metastases, rather than the primary tumor and majority of metastases are due to the recurrence and progression of non-metastatic disease. Current adjuvant treatments, such as chemotherapy and radiation, have severe side effects and may result in overtreatment and drug resistance.

Since greater than 90% of patients are diagnosed between stages I-III and have minimal residual disease after treatment, there is an opportunity to treat patients with an autologous breast cancer vaccine. Autologous vaccines under development have a …

A Wellness Profile Of Student-Veterans At 4-Year Higher Education Institutions: The Role Of Gender, Combat Tours, And Deployment, William Monroe Heath

Graduate Theses and Dissertations

Afghanistan and Iraq war student-veterans have increased in population on college campuses in the United States (U.S.) as a result of using the Post 9/11 GI Bill. Healthy Campus 2020 (American College Health Association, 2012) highlighted the importance of college student wellbeing, health promotion and disease prevention, while the U.S. Department of Veteran Affairs has called for an approach to services that is strength based (United States Department of Veteran Affairs, 2008). To date, however, wellness studies conducted on Iraq and Afghanistan student-veterans using an evidenced-based model of holistic wellness have not been conducted. This study was designed to address …

Acanthamoeba Spp. As Reservoirs For Transmission Of Norovirus, Tun-Yun Hsueh

Graduate Theses and Dissertations

Human noroviruses (HuNoV) are the most common cause of foodborne disease outbreaks in the United States and the most common food commodities implicated in HuNoV outbreaks are leafy greens; however, the vehicle of transmission and point of contamination are often unknown. Here, we hypothesize that common free-living amoebae (FLA) ubiquitous in the environment may act as reservoirs of HuNoV and facilitate the transmission of these pathogens to fresh produce. The objective of this research was to first evaluate the interaction/association between HuNoV surrogates and Acanthamoeba by incubating them together and analyzing virus titer associated with amoeba through an 8 day …

Measuring The Role Of Inhibition In Auditory Discrimination In Mice, Tazima Nur

Graduate Theses and Dissertations

Understanding how inhibitory neurons affect sensory information processing in the cerebral cortex is an ongoing goal of both neuroscience and statistical physics research. In this master's thesis research project, an experimental system has been designed and built for studying how auditory dynamic range depends on inhibitory neurons, based on observations of mouse behavior. In this thesis, firstly, the topic of inhibition and information processing has been introduced. Then two papers related to inhibition and dynamic range has been reviewed in detail. One of the papers is an experimental work that analyzes the affect of inhibition on dynamic range. The other …

Improving Adjunct Nursing Instructors' Knowledge Of Student Assessment In Clinical Courses, Kelly Vowell Johnson

Graduate Theses and Dissertations

Utilization of adjunct nursing instructors to teach clinical courses is a common occurrence in nursing programs. Adjunct clinical instructors are often expert clinicians, but they have limited experience in teaching and lack the expertise needed to be successful in the educator role, such as knowledge of student assessment. Faculty development programs that focus on student assessment can provide adjunct clinical faculty members with the necessary knowledge to become effective educators and ensure student, faculty, and program success.

The purpose of this study was to examine to what extent a faculty development workshop on evaluating students in clinical courses affected adjunct …

Large-Scale Solution Approaches For Healthcare And Supply Chain Scheduling, Ridvan Gedik

Graduate Theses and Dissertations

This research proposes novel solution techniques for two real world problems. We first consider a patient scheduling problem in a proton therapy facility with deterministic patient arrivals. In order to assess the impacts of several operational constraints, we propose single and multi-criteria linear programming models. In addition, we ensure that the strategic patient mix restrictions predetermined by the decision makers are also enforced within the planning horizon. We study the mathematical structures of the single criteria model with strict patient mix restrictions and derive analytical equations for the optimal solutions under several operational restrictions. These efforts lead to a set …

Recruitment, Retention, Job Satisfaction Of Nurse Educators In Arkansas, Peggy B. Lee

Graduate Theses and Dissertations

Facing a crucial shortage of nurses and nurse educators, administrators of colleges and universities need to explore employment and individual characteristics that are related to recruitment and retention of nurse educators. Adding to the nurse and nurse educator shortage is the concern that the population of the United States is aging which creates a growing demand for more RNs. A further issue that complicates the nurse and nurse educator shortage is that nursing education is not producing enough RNs. Schools of nursing are positioned to have to prepare more new nurse graduates over the next decade in an effort to …

New Bsn Nurse Informatics Competencies: Perceptions Of Academic Preparedness For Practice, Teri Montgomery Boyd

Graduate Theses and Dissertations

One rapidly expanding nursing program at a public research university in the mid-south region of the United States served as the site for this study. Recent nurse graduates were selected in a purposeful sampling from alumni who had conferred degrees from May 2007 through May 2013. Sixty-three graduates completed the online survey in Phase I and five recent graduates participated in semi-structured interviews during Phase II of the study. Data were collected utilizing a mixed methods design. Descriptive statistics explained survey and interview results. Of the knowledge, skills, and attitudes (KSAs), attitude competencies reported the highest means and were very …

The Effect Of School And Neighborhood Environmental Factors On Childhood Obesity, Yiwei Qian

Graduate Theses and Dissertations

The dissertation consists of three chapters exploring the effect of school program and neighborhood environment on childhood obesity outcome using individual panel data set of Arkansas public schoolchildren.

The first chapter (Section 2) investigates how the Fresh Fruit and Vegetable Program (FFVP), a program that provides funding for the distribution of free fresh fruits and vegetables to students in participating schools, affects childhood obesity. We combine matching methodology and difference-in-differences analysis to estimate the effect of the FFVP on childhood BMI outcomes. Estimates of the FFVP effect are sensitive to different matching methods. Methods that provide a good balance between …

A Content And Visual Analysis Of Promotional Pieces Used To Promote The Arkansas Soybean Promotion Board, Amy Hughes

Graduate Theses and Dissertations

A communications campaign was developed for a commodity promotion board in the southern region of the United States with promotional pieces produced and disseminated by a third-party communications group to reach the general public, soybean producers, and animal agriculture producers target audiences through key themes and messages. A systematic, content-driven approach assessed the potential impact on perceptions of individuals. This study utilized a content and visual analysis based on semiotic theory to analyze creative pieces and focus groups to assess content quality and impact.

Many of the creative pieces displayed too many themes, and the themes did not accurately represent …

Chitosan Reduces Enteric Colonization Of Campylobacter In Young Chickens, But Not On Post-Harvest Chicken Skin Samples, Hanna Royce Arambel

Graduate Theses and Dissertations

Worldwide, Campylobacter is one of the leading causes of foodborne bacterial gastroenteritis causing an estimated 1.3 million infections in the United States alone. Consumption and/or cross-contamination of raw or undercooked poultry products have been linked as the most common source of Campylobacter infection, making the poultry industry a target for Campylobacter reduction strategies. Campylobacter is prevalent in most poultry flocks in the United States, with as many as 90% of flocks Campylobacter-positive at the time of slaughter. It is estimated that a reduction of Campylobacter in poultry would greatly reduce the risk of campylobacteriosis in humans. Unfortunately, there are …

Psychometric Evaluation Of A Standardized Set Of Alcohol Cue Photographs To Assess Craving, David Edison Lovett

Graduate Theses and Dissertations

To date, research paradigms using alcohol-related stimuli have been limited in their reliability and generalizability due to a lack of published studies examining the psychometric properties of alcohol cues. The primary aim of the current study was to examine the factor structure of a set of alcohol cues and the associated validity and reliability of examining craving for alcohol in an alcohol cue-reactivity paradigm. Participants (N = 195, 74.4% male, 56.4% Asian, Asian American, or Pacific Islander) completed a web-based survey in which valence, arousal, and craving ratings were obtained following presentation of picture cues. Prior to picture cues, participants …

Impact Of Healthy Lifestyle Choices On Smoking Behavior Among College Students Who Smoke Cigarettes, Mitchell Jenkins

Graduate Theses and Dissertations

This dissertation examines the impact certain healthy lifestyle choices had on smoking behavior among college students who smoke cigarettes. Even with continued reduction in prevalence, cigarette smoking is still the leading cause of preventable death in America. With that in mind, it is important to continue to identify factors that relate to decreased tobacco usage. Secondary data from the American College Health Association's bi-yearly National College Health Assessment was used for this study. This assessment/survey encompasses college students' habits, behaviors, and perceptions regarding prevalent health topics. The sample for this study consisted of 14,515 college students who identified themselves as …

Investigation Of Rice Bran Derived Anti-Cancer Pentapeptide For Mechanistic Potency In Breast Cancer Cell Models, Ruiqi Li

Graduate Theses and Dissertations

Bioactive peptides derived from food sources with anti-proliferative properties against cancer have drawn more attention in recent years. A pentapeptide derived from rice bran has shown anti-proliferative propertiesagainst human breast cancer cells. The objective of this study was to investigate the mechanistic action of the pentapeptide-induced apoptosis in breast cancer cell models (MCF-7 and MDA-MB-231). The growth inhibition activity of the pentapeptide was

evaluated by MTS[3-(4,5-dimethylthiazol-2-yl)-5-(3- arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assayand trypan blue assay in a dose- and time-dependent manner.

The apoptotic properties of pentapeptide-induced apoptosis on cancerous breast cells were evaluated by morphological changes, DNA fragmentation, and caspases-3/7, -8,and -9 …

The Efficacy Of Probiotic Bacterial Isolates In Reducing Cecal Camylobacter Colonization In Broiler Chickens, Komala Arsi

Graduate Theses and Dissertations

Campylobacter is the leading cause of foodborne illness worldwide and human illnesses are often associated with consumption of poultry or poultry products. Many strategies have been tried to eliminate Campylobacter from poultry with limited success. One of the strategies to reduce Campylobacter colonization in poultry is by use of probiotics. We conducted 2 separate studies to evaluate the efficacy of probiotics against Campylobacter in broiler chickens. For our first study, GRAS (Generally Regarded as Safe) bacteria were isolated from healthy chickens and tested their efficacy against Campylobacter in vitro. Twenty six isolates with in vitro anti-Campylobacter activity were …

The Impact Of Body Image On Women In Later Life: Effects On Quality Of Life And Body Perception, Rachel L. Avants

Graduate Theses and Dissertations

Physical, mental, and emotional changes can occur throughout the aging process, making it important to treat different age groups as separate populations when researching body image. Yet, very few instruments have been validated for middle-aged and older adults. An online survey was used to perform a validation of the Body Image Quality of Life Inventory (BIQLI) and examine demographic relationships, with a sample of 947 women, ages 40-79, from across the United States. A principal component analysis (PCA) and convergent validity supported the use of the BIQLI for women ages 40 to 79. A one-factor model was validated for comparing …

Power To The People? An Evaluation Of State-Level Environmental Justice Policies, Angela Michelle Hines

Graduate Theses and Dissertations

Communities of color in America lacking economical, educational, and political power have been largely invisible in the process of making major policy decisions. This is lack of access to decision-making venues has been viewed by many as the reason behind marginalized populations bearing the brunt of many societal burdens. The Environmental Justice Movement legitimized the claims of inadequate access to the decision-making process concerning environmental conditions in which African-Americans lived and worked. Through the use of disruptive actions reminiscent of those used throughout the Civil Rights Movement, the plight of communities plagued by the daily presence of hazardous waste gained …

"The Claims Of Religion Upon Medical Men": Protestant Christianity And Medicine In Nineteenth-Century America, Ronald James Gordon

Graduate Theses and Dissertations

This is the first study to examine how pastors lost authority over bodily healing in the nineteenth century. I argue that clergymen adapted to the scientific and cultural developments of the post-Darwin era by encouraging a separation of bodily healing and spiritual healing that was unprecedented in Christianity. In 1840, ministers wielded much more authority than physicians, who still practiced a heroic medicine whose foundations predated Christ. Ministers insisted that doctors' value lay in their willingness to be proselytizing Christians. The doctor who wanted to reach the upper echelon of his profession had to save the souls of the wayward …