Medicine and Health Sciences Commons^™

Store-Operated Calcium Channels In The Function Of Intracardiac Neurons, Timetria Bonds

USF Tampa Graduate Theses and Dissertations

Proper autonomic regulation of mammalian cardiac function is dependent upon very complex and precise communication among the intracardiac ganglia and individual neurons within the ganglia. An array of neuromodulators is found within the ganglia that direct neuronal activity by modulating the movement of calcium. The current study determines that opioidergic agonists, which have been found to contribute to severe cardiac disease states and intracellular calcium mobilization, are also responsible for changes in the function of the intracardiac neuron via their effects on store-operated calcium channels (SOCs).

Previous studies suggest that phosphorylation plays a role in SOC regulation. Using Fura-2 calcium …

An Examination Of Diet, Acculturation And Risk Factors For Heart Disease Among Jamaican Immigrants, Carol Renee Oladele

USF Tampa Graduate Theses and Dissertations

Background: The South Florida region is home to over 85,000 Jamaican immigrants. Yet, little is known about the dietary intakes and predictors of risk of disease within this immigrant group. An assessment of dietary intakes and the development of dietary intake methodologies specific to the Jamaican population was important as it permitted accurate estimation of the nutrient intakes of this immigrant population whose dietary habits are not well documented. In addition, nothing is known about the prevalence of risk factors for heart disease or factors influencing risk factors among this immigrant group. The purpose of this study was to assess …

"Not If, But When": Sex, Risk, And Trust In Timing Gardasil Vaccine Decisions, An Exploratory Study Among Healthcare Providers And Middle-Class Parents In The U.S., Kathleen Marie Brelsford

USF Tampa Graduate Theses and Dissertations

This dissertation research explores how values regarding sexuality, morality, responsibility, protection, trust, and risk — expressed through parent, daughter, and healthcare provider relationships and interactions — inform parental decisions regarding the Gardasil® vaccine. In particular, the research examines the competing and conflicting meanings that parents and providers ascribe to vaccination and how actors position the vaccine within a wider set of negotiated, value–laden discourses. Because these narratives are situated within a larger structural field that shapes the landscape in which providers and parents interact, relevant historical and structural factors, including vaccine policy, cost, and compensation are discussed.

Prophylactic, Risk-Reducing Surgery In Unaffected Brca-Positive Women: Quality Of Life, Sexual Functioning And Psychological Well-Being, Sharon Tollin

USF Tampa Graduate Theses and Dissertations

Women with an inherited BRCA mutation are at significantly increased risk for breast and ovarian cancer, often diagnosed at an earlier age than sporadic cancers. Prophylactic surgery, with bilateral mastectomy and/or bilateral prophylactic salpingo-oophorectomy, represents an option for risk reduction. The purpose of this study was to explore quality of life, sexual functioning, menopausal symptoms, psychological well-being and satisfaction with risk management decisions for BRCA-positive women ages 21 to 50 (M = 38.4 years), without a personal history of cancer. A web-based, cross-sectional study design was utilized to compare women opting for any prophylactic surgery (n = 160) with those …

Dengue Fever In Tegucigalpa, Honduras: Use Of The Explanatory Model In A Sample Of Urban Neighborhoods To Contextualize And Define Dengue Fever Among Community Participants, Jose Enrique Hasemann

USF Tampa Graduate Theses and Dissertations

This project elucidated the explanatory model of dengue fever held by members of urban communities in Tegucigalpa, Honduras. The study was conducted over a four-month period from May-August of 2011, and it was divided into two stages. The first stage of the project consisted of volunteer participation with dengue fever surveillance brigades in the three communities with the highest incidence of dengue fever during the beginning of 2011. This initial stage employed participant observation as its research method. The second stage was conducted in a different community within Tegucigalpa. The primary research methods employed during the second stage of the …

Accuracy Of Educator Nominations In Identifying Students With Elevated Levels Of Anxiety And Depression, Jennifer Cunningham

USF Tampa Graduate Theses and Dissertations

Internalizing disorders, specifically depression and anxiety, affect up to 18% and 33% of youth, respectively (Costello, Egger, & Angold, 2005b). Schools have become a major provider of mental health services to children, primarily in attempts to overcome barriers to receiving community services (Farmer, Burns, Philip, Angold, & Costello, 2003). As such, it is important that schools have effective mechanisms in place to accurately identify students who may be in need of such services. The current study examined the accuracy of one such method, educator nominations (including from both teachers and school-based mental health professionals) in identifying students who self-report elevated …

The Role Of Male Partners In Childbirth Decision Making: A Qualitative Exploration With First-Time Parenting Couples, Sharon Dejoy

USF Tampa Graduate Theses and Dissertations

Fathers' attendance at childbirth is almost universal in the United States, but few researchers have addressed the role that males play in childbirth decision making. The number of technological interventions available to birthing women is increasing, as is the utilization of those interventions. The degree to which women choose or agree to these interventions plays a major role in individual and societal health outcomes. Therefore, health care policy makers and educators must find ways to help childbearing couples navigate the complex maze of decisions related to childbirth. However, policies and programs to increase shared decision making may not function as …

The Holistic Complementary Structure Of Western Bio-Medicine And Traditional Healing And Achieving Complete Health, Candace Gail Oubre

USF Tampa Graduate Theses and Dissertations

Achieving complete health requires a deep understanding of complementary cultural competency sensitivity between physician and patient. This may include but is not limited to access to preventative health care resources, access to health educational resources and access to cultural healing resources, for example, shamans, Ayurvedic physicians, and herbal healers. Advocates of cultural competency emphasize great importance on knowledge of the patients' cultural background; however, the transcendence of this knowledge can be explained further through complementary cultural competency sensitivity. This is when the cultures of the physician and patient complement each other in terms of understanding what is in the patients' …

Emotional Reactivity And Regulation In Current And Remitted Depression: An Event Related Potential Study, Lauren M. Bylsma

USF Tampa Graduate Theses and Dissertations

Major Depressive Disorder (MDD) is thought to be characterized by emotion regulation deficits, including decreased use of adaptive strategies such as reappraisal, but little is known about the exact nature of these deficits and whether or not they are specific to the depressed mood state. The late positive potential (LPP) is a sustained positive deflection of the event-related potential (ERP) associated with responding to emotionally-valenced stimuli, and reappraisal strategies have been found to reduce LPP magnitude in response to emotional stimuli in healthy individuals, but this effect has not been examined in MDD. This study utilized ERPs to examine emotional …

Characterization Of The Serologic Responses To Plasmodium Vivax Dbpii Variants Among Inhabitants Of Pursat Province, Cambodia, Samantha Jones Barnes

USF Tampa Graduate Theses and Dissertations

The Plasmodium vivax Duffy Binding Protein (DBP) is the ligand in the major pathway for P. vivax invasion of human reticulocytes, making it an appealing vaccine candidate. Region II of DBP (DBP-RII) is the minimal portion of the ligand that mediates recognition of the Duffy Antigen Receptor for Chemokines (DARC receptor) on the reticulocyte surface and constitutes the primary vaccine target. Analysis of natural variation in the coding sequences of DBP-RII revealed signature evidence for selective pressure driving variation in the residues of the putative receptor-binding site. We hypothesize that anti-DBP immunity in P. vivax infections is strain-specific and hindered …

Discovery Of A Functional Ecdysone Response Element In Brugia Malayi, Tracy Enright

USF Tampa Graduate Theses and Dissertations

The aim of this study was to determine whether functional ecdysone response elements (EcREs) exist within the genome of Brugia malayi, a parasitic nematode that causes lymphatic filariasis. The hypothesis that EcREs exist in B. malayi stemmed from previous demonstration of a functional ecdysone response system in B. malayi (Tzertzinis et al., 2010). Real-time PCR (qPCR) experiments were conducted to measure gene expression levels for twelve genes proximal to five putative EcREs in 20-OH ecdysone treated and untreated B. malayi embryos. Seven genes showed consistent upregulation with 20-OH ecdysone treatment. Each of the five putative EcREs had at least one …

An Observation Of Immunological Effect, A Diet Enhanced With Spirulina And Treatment With Fractalkine In Models Of Parkinson's Disease, Mibel M. Pabón

USF Tampa Graduate Theses and Dissertations

In my dissertation research we used use human wild type α-synuclein gene expression using an adeno-associated viral vector (AAV9) that induced a slowly progressive loss of dopamine (DA) neurons in the Substantia nigra (SN) as one of our animal model of Parkinson’s disease (PD). It is our hypothesis that neuroinflammation predisposes the brain to susceptibility to neurodegenerative diseases. Thus we examined the progression of a PD lesion and examined the manipulations of the immune system to understand further the inflammatory role when we administered exogenous soluble fractalkine.

The specific etiology of neurodegeneration in PD is unknown, but the inflammatory mechanisms …

Evaluation Of Preference For Exergames Among Elementary Students, Christie Ann Cacioppo

USF Tampa Graduate Theses and Dissertations

Obesity in children is a nationwide problem. Physical activity is one way to help children stay fit and prevent obesity. Unfortunately, access to technology involving sedentary behavior is easier than ever. Fortunately, for this generation of students there is a way to combine physical activity and technology through exergaming. In order to encourage students to participate in exergaming, the students should be able to play games they prefer. The purpose of this study was to apply a paired choice preference assessment, rank order card sort, and social validity surveys to determine the preference order of six exergames for six elementary …

Utilizing Probabilistic Reinforcement To Enhance Participation In Parent Training, Errity Jones

USF Tampa Graduate Theses and Dissertations

Parental participation in parent training programs is necessary for success in behavioral parent training. Prior literature has demonstrated probabilistic reinforcement as an effective intervention for improving a wide variety of behaviors. In the present study, a probabilistic reinforcement program (i.e., lottery) was implemented in order to evaluate its efficacy as part of a behavioral parent training program. The behaviors targeted for increase included attendance, participation, homework completion, and performing role-plays or completing in-class assignments for two 10 week Tools for Positive Behavior Change courses. Participants earned lottery tickets for each of the dependent measures, and drawings took place at the …

Modeling And Surveillance Of Pandemic Influenza Outbreaks, Diana Prieto

USF Tampa Graduate Theses and Dissertations

Pandemic outbreaks are unpredictable as to their virus strain, transmissibility, and impact on our quality of life. Hence, the decision support models for mitigation of pandemic outbreaks must be user-friendly and operational, and also incorporate valid estimates of disease transmissibility and severity. This dissertation research is aimed at 1) reviewing the existing pandemic simulation models to identify their implementation gaps with regard to usability and operability, and suggesting research remedies, 2) increasing operability of simulation models by calibrating them via an epidemiological model that estimates infection probabilities using viral shedding profiles of concurrent pandemic and seasonal influenza, and 3) developing …

Does D-Cycloserine Augmentation Of Cbt Improve Therapeutic Homework Compliance For Pediatric Obsessive Compulsive Disorder?, Jennifer M. Park

USF Tampa Graduate Theses and Dissertations

D-cycloserine (DCS), a partial agonist that acts on the N-methyl-D-aspartate (NMDA) receptor of the glutamatergic receptor complex, may enhance fear extinction learning during exposure-based therapy. Clinical studies in adults with obsessive-compulsive disorder (OCD) and non-OCD anxiety disorders - and a recent trial in pediatric OCD - have shown that DCS can improve treatment response to exposure therapy relative to placebo and exposure therapy. Some have hypothesized that improved treatment response is a function of increased compliance and engagement in therapeutic homework tasks, a core component of behavioral treatment. The present study examined the relationship between DCS and homework compliance in …

The Role Of Lipoproteins/Cholesterol In Genomic Instability And Chromosome Mis-Segregation In Alzheimer's And Cardiovascular Disease, Antoneta Granic

USF Tampa Graduate Theses and Dissertations

Several lines of evidence link Alzheimer's disease (AD) to atherosclerosis (CVD), including that elevated low density lipoprotein (LDL)-cholesterol is a common risk factor. Development of genomic instability could also link the two diseases. Previous fluorescence in situ hybridization (FISH) analyses revealed a clonal expansion of aneuploid smooth muscle cells underlying atherosclerotic plaques. Likewise, cellular and mouse models of AD revealed tau-dependent mitotic defects and subsequent aneuploidy partly resulting from amyloid-beta (A&beta) interference with microtubule (MT) stability, and specific MT motors function. Moreover, AD patients develop aneuploid/hyperploid cells in brain and peripheral tissues, implicating similar mechanism that may lead to apoptosis …

Assessment Of The Dependence Of Ventilation Image Calculation From 4d-Ct On Deformation And Ventilation Algorithms, Kujtim Latifi

USF Tampa Graduate Theses and Dissertations

Ventilation imaging using 4D-CT is a convenient and cost effective functional imaging methodology which might be of value in radiotherapy treatment planning to spare functional lung volumes. To calculate ventilation imaging from 4D-CT we must use deformable image registration (DIR). This study validates the DIR methods and investigates the dependence of calculated ventilation on DIR methods and ventilation algorithms.

The first hypothesis is if ventilation algorithms are robust then they will be insensitive to the precise DIR used provided the DIR is accurate. The second hypothesis is that the change in Houndsfield Unit (HU) method is less dependent on the …

Repeated Binge Pattern Ethanol Administration During Adolescence Or Adulthood: Long-Term Changes In Voluntary Ethanol Intake And Mesolimbic Dopamine Functionality In Male Rats, Antoniette Michelle Maldonado-Devincci

USF Tampa Graduate Theses and Dissertations

Binge alcohol consumption is a rising concern in the United States, especially among adolescents as during this developmental period alcohol use is usually initiated and has been shown to cause detrimental effects on brain structure and function. These findings have been established through the use of binge models in animals, where animals are repeatedly administered high doses of ethanol typically over a period of three or four days. While such work has examined the effects of a four-day and repeated three-day binge, there has been almost no work conducted aimed at investigating the long-term behavioral and neurochemical and/or functional consequences …

The Impact Of Hospice And Dementia Special Care Units On End-Of-Life Care For Individuals With Dementia, Catherine Elizabeth Mccarty

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Hospice and Dementia Special Care Units (DSCUs) have been shown to be gold standards of care for individuals who are dying and for those with dementia in nursing homes. Using a retrospective administrator and family survey, this study investigated whether the processes of care used in hospice and DSCUs are associated with increased quality of end of life (EOL) care for individuals with dementia. A convenience sample of 17 facilities in four states (FL, PA, MD and MA) was included in the study. Nursing home administrators were surveyed between September 2008 and October 2009. The administrators identified 116 family …

Visual Search For Smoking Stimuli: Detection And Distraction, Jason A. Oliver

USF Tampa Graduate Theses and Dissertations

Extensive research has shown that the attentional systems of addicted individuals are biased towards drug-related stimuli, but despite several decades of effort these results have frequently been inconsistent. Though commonly believed to result from addiction and dependence, cognitive research would suggest that frequent exposure to drug-related stimuli could affect the attentional processing of drug-related cues even if no actual drug use occurs. The present investigation examined attentional bias for smoking cues using a novel visual search paradigm amongst smokers currently in nicotine withdrawal and fully satiated smokers, as well as a non-smoker control group. Variables related to smoking behavior, as …

Decision Aid Models For Resource Sharing Strategies During Global Influenza Pandemics, Alfredo Santana Reynoso

USF Tampa Graduate Theses and Dissertations

Pandemic influenza outbreaks have historically entailed significant societal and economic disruptions. Today, our quality of life is threatened by our inadequate preparedness for the imminent pandemic. The key challenges we are facing stem from a significant uncertainty in virus epidemiology, limited response resources, inadequate international collaboration, and the lack of appropriate science-based decision support tools. The existing literature falls short of comprehensive models for global pandemic spread and mitigation which incorporate the heterogeneity of the world regions and realistic travel networks. In addition, there exist virtually no studies which quantify the impact of resource sharing strategies among multiple countries. This …

Identification Of Patient Recovery Patterns After Cardiovascular Surgery Based On Laboratory Tests Results, Alcides Ricardo Santander Mercado

USF Tampa Graduate Theses and Dissertations

In this dissertation is proposed a methodology to identify patient's recovery patterns after cardiovascular surgery based on laboratory tests results. The main purpose is to enhance the understanding of the manifestations of postsurgical complications in patients who underwent cardiovascular surgery. The analysis of patients' recovery process is based on the relationship between plasma calcium, ionized calcium and platelet count over time.

Laboratory results from the James A. Haley Veterans' Hospital databases, related to patients admitted to the Surgical Intensive Care Unit (SICU) after cardiac surgery (coronary artery bypass, aortic value replacement and mitral valve replacement), are used. These databases contain …

Food Deserts' And 'Food Swamps' In Hillsborough County, Florida: Unequal Access To Supermarkets And Fast-Food Restaurants, Dana Beth Stein

USF Tampa Graduate Theses and Dissertations

Research has shown that the suburbanization of supermarkets has created `food deserts', defined as areas where socially disadvantaged individuals lack access to nutritious food outlets. Additionally, the growing presence of fast-food restaurants has created `food swamps', or areas where socially disadvantaged individuals encounter an overabundance of unhealthy food outlets. While previous studies have analyzed either `food deserts' or `food swamps' using conventional statistical techniques, a more comprehensive approach that includes samples of both healthy and unhealthy entities and considers the variety of available food options is necessary to improve our understanding of the local food environment and related disparities.

This …

Tarnished Golden Years: Older Offenders With Mental Health Problems And Late Life First Time Offenders, Brianne Stanback

USF Tampa Graduate Theses and Dissertations

Older offenders (offenders 50 years and older) are a distinct groups within the U.S. correctional system. Studies 1 and 2 were intended to investigate mental health and stressors among jailed older offenders.

Study 1 examined the prevalence rates of mental health disorders and explored the relationship between mental health disorders and stressors. Participants 50 years and older (N=330) from the 2002 wave of the Survey of Inmates in Local Jails (SILJ) were studied via descriptive analysis, factor analysis, and a logistic regression to determine which variables were related to the probability of having a mental health problem. 65.8% of participants …

The Role Of Contextual Associations In The Selection Of Objects, Noah Patrick Sulman

USF Tampa Graduate Theses and Dissertations

This paper describes a sequence of experiments addressing basic questions about the control of visual attention and the relationship between attention and object recognition. This work reviews compelling findings addressing attentional control on the basis of high-level perceptual properties. In five experiments observers were presented with a rapid sequence of object photographs and instructed to either detect or selectively encode a verbally cued object category. When these object categories (e.g. "baseball") were preceded by contextual images associated with a given object category (e.g. "baseball diamond"), observers were less likely to accurately report information about the target item. This effect obtained …

School Psychologists' Communication And Collaboration With Community-Based Mental Health Professionals, Audra St. John Walsh

USF Tampa Graduate Theses and Dissertations

Although collaboration between school psychologists and community-based mental health professionals is essential in the provision of comprehensive and effective mental health services for youth with intensive mental health needs, youth may not receive the full benefit of these coordinated efforts, as collaboration may not occur as often as it should. This study investigated the frequency of communication and collaboration between school- and community-based professionals, the purposes and methods of communication, and the benefits and barriers to collaboration. Survey data from 80 members of the Florida Association of School Psychologists were collected and analyzed. Forty-three percent of school psychologists reported communicating …

An Evaluation Of Booster Training Using Video Modeling With Foster Parents, Anna Katherine Caravello

USF Tampa Graduate Theses and Dissertations

In order for foster parents to be prepared to manage problem behavior and develop a positive relationship with a child in their home, local foster care agencies require these parents to attend parent training classes. Unfortunately, even foster care agencies that offer empirically validated parent training courses are unable to prevent the parents' performance from decreasing over time (Cowart, Iwata, & Poynter, 1984; Forehand & King, 1977; Mueller et al., 2003). However, researchers have created booster training sessions to counteract this issue. Booster training sessions allow participants to attend a brief refresher course on skills they have previously learned. Another …

Making A Splash: Eliminating Water Phobia And Increasing Confident Water Skills, Paula Elizabeth Chan

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to utilize a multi-component intervention to increase confident water skills for three typically developing children, and to evaluate whether fearful behaviors decreased as the intervention progressed. The intervention, comprised of goal setting, self-monitoring, behavioral skills training and positive reinforcement, was used in community pools to teach basic water skills. Results suggest the intervention was successful in increasing confident water skills, while intervals with positive affect and fearful behaviors demonstrated variability.

The Impact Of Pharmacy Work Design On Pharmacist Productivity, Nicholas Allen Coblio

USF Tampa Graduate Theses and Dissertations

Healthcare costs in the United States continue to grow at an alarming rate. Concerning the cost of medications, there are a number of factors that drive these costs. While personnel costs are not the largest of these, they do contribute a significant portion. The cost of the cognitive component of order processing by pharmacists can range from three dollars to over six dollars per prescription depending on the production throughput of the pharmacist.

Studies at the organization which was the focus of the research, as well as reports in the literature, indicated that work disruption and other environmental factors could …