Medicine and Health Sciences Commons^™

A Selective Gβγ-Linked Intracellular Mechanism For Modulation Of A Ligand-Gated Ion Channel By Ethanol, Gonzalo E. Yevenes, Gustavo Moraga-Cid, Robert W. Peoples, Günther Schmalzing, Luis G. Aguayo

Biomedical Sciences Faculty Research and Publications

The current understanding about ethanol effects on the ligand-gated ion channel (LGIC) superfamily has been restricted to identify potential binding sites within transmembrane (TM) domains in the Cys-loop family. Here, we demonstrate a key role of the TM3–4 intracellular loop and Gβγ signaling for potentiation of glycine receptors (GlyRs) by ethanol. We discovered 2 motifs within the large intracellular loop of the GlyR α₁ subunit that are critical for the actions of pharmacological concentrations of ethanol. Significantly, the sites were ethanol-specific because they did not alter the sensitivity to general anesthetics, neurosteroids, or longer n-alcohols. Furthermore, Gβγ scavengers …

Racial And Ethnic Disparities In Nontraumatic Dental-Condition Visits To Emergency Departments And Physician Offices In The Wisconsin Medicaid Program, Christopher Okunseri, Nicholas M. Pajewski, David C. Brousseau, Sandy Tomany-Korman, Andrew Snyder, Glenn Flores

School of Dentistry Faculty Research and Publications

Background

Nontraumatic dental condition (NTDC) visits occur in emergency departments (EDs) and physician offices (POs), but little is known about factors associated with NTDC visit rates to EDs and POs.

Methods

The authors analyzed all Medicaid dental claims in Wisconsin from 2001 through 2003 to examine factors associated with NTDC visits to EDs and POs. They performed bivariate and multivariable analyses. The independent variables they examined included race/ethnicity, age, sex, dental health professional shortage area (DHPSA) designation and urban influence code for county of residence.

Results

The authors evaluated 956,774 NTDC visits made during 1,718,006 person-years; 4.3 percent of visits …

Altering Occlusal Vertical Dimension Provisionally With Base Metal Onlays: A Clinical Report, Soni Prasad, Jennifer Kuracina, Edward A. Monaco, Jr.

School of Dentistry Faculty Research and Publications

This article presents a method for altering occlusal vertical dimension (OVD) to restore dentitions with limited restorative space due to loss of tooth structure. A provisional increase in OVD is achieved using predominately base metal onlays, which offer advantages over conventional techniques. The onlays are resistant to wear, may be bonded well to nonrestored and restored tooth surfaces with resin cements, and provide a fairly reversible method of increasing OVD. A patient situation is presented which demonstrates the use of provisional base metal onlays in complete mouth rehabilitation.

The Influence Of Religiosity, Gender, And Language Preference Acculturation On Sexual Activity Among Latino/A Adolescents, Lisa Edwards, Richard Fehring, Keyona M. Jarrett, Kristin Haglund

College of Nursing Faculty Research and Publications

The purpose of this study was to determine the main and interactive effects of religiosity, gender, and language preference acculturation on sexual activity among 570 Latino/a adolescents from the 2002 National Survey of Family Growth. Results indicated that adolescents who viewed religion as very important, had frequent church attendance, and had more traditional attitudes on sexuality were less likely ever to have sex compared with adolescents who were less religious. Those with frequent church attendance and high traditional attitudes had fewer lifetime and recent sex partners. Unassimilated religious youth were less likely ever to have sex, had fewer lifetime and …

Coping Difficulties After Hospitalization, Judith Fitzgerald Miller, Linda Piacentine, Marianne Weiss

College of Nursing Faculty Research and Publications

Coping difficulties of 113 adults 3 weeks after hospital discharge were identified using the Post-Discharge Coping Difficulty Scale and a brief focused telephone interview (11-item guide). Overall, low difficulty scores were reported (M = 23.9, SD = 18.2, range = 0 to 100). Qualitative data reveal specific coping difficulties in the categories of stressors, specific difficulties, caring for self, managing the condition, family, advice needed, contact with the health care system, and what they wished they knew before discharge. A core theme of biographical reconstruction emerged.

Reducing Sexual Risk With Practice Of Periodic Secondary Abstinence, Kristin Haglund

College of Nursing Faculty Research and Publications

Objective: Test a novel intervention to help sexually experienced girls increase abstinence behaviors and attitudes. Design: A quasi-experimental repeated measures design using qualitative and quantitative data. Setting: Two alternative public schools. Participants: Thirty-three females whose mean age was 16 and who were 79% African American participated. Most (79%) had experienced a pregnancy. Intervention: A 6 session, weekly, interactive intervention was delivered. Data were collected at baseline, last session, and at 5 and 7 month follow-ups. Main Outcome Measures: Measured outcomes related to abstinence included participants’ reasons, behaviors, stages of change, and attitudes. Results: The most common reason for abstinence was …

Efficacy Of The Marquette Method Of Natural Family Planning, Richard Fehring, Mary Schneider, Mary Lee Barron

College of Nursing Faculty Research and Publications

Purpose: To determine the effectiveness of the Marquette Method (MM) of natural family planning (NFP) as a method of avoiding pregnancy. Study Design and Methods: This was a 12-month retrospective evaluation of the MM system of NFP. Two hundred and four women (mean age, 28.6 years) and their male partners (mean age, 30.3 years) who sought to learn a method for avoiding pregnancy with the MM from four clinical sites were taught to track their fertility by self-observation of cervical mucus, by use of an electronic monitor that measures urinary levels of estrone-3-glucuronide and luteinizing hormone, and by use of …

Efficacy Of The Marquette Method Of Natural Family Planning, Richard Fehring, Mary Schneider, Mary Lee Barron

College of Nursing Faculty Research and Publications

Purpose: To determine the effectiveness of the Marquette Method (MM) of natural family planning (NFP) as a method of avoiding pregnancy.

Study Design and Methods: This was a 12-month retrospective evaluation of the MM system of NFP. Two hundred and four women (mean age, 28.6 years) and their male partners (mean age, 30.3 years) who sought to learn a method for avoiding pregnancy with the MM from four clinical sites were taught to track their fertility by self-observation of cervical mucus, by use of an electronic monitor that measures urinary levels of estrone-3-glucuronide and luteinizing hormone, and by use of …

A Technique For Duplicating A Fixed Complete Denture, Soni Prasad, Edward A. Monaco, Jr.

School of Dentistry Faculty Research and Publications

The fixed implant prosthesis often requires repair due to fracture and/or wear of acrylic resin teeth. Repair of such a prosthesis, although simple, requires retaining the prosthesis to be revised in the laboratory. In order to reveneer the implant prosthesis, a provisional restoration should be fabricated for the patient that provides similar function and esthetics. This article presents a technique for duplicating the existing fixed implant-supported prosthesis prior to repair/reveneer, thus providing a smooth transition from the provisional to the definitive prosthesis.

Effect Of Phosphate Group Addition On The Properties Of Denture Base Resins, Gaurav Puri, David W. Berzins, Virendra B. Dhuru, Periathamby A. Raj, Sameer K. Rambhia, Gunjan Dhir, Andrew R. Dentino

School of Dentistry Faculty Research and Publications

Statement of problem

Acrylic resins are prone to microbial adherence, especially by Candida albicans. Surface-charged resins alter the ionic interaction between the denture resin and Candida hyphae, and these resins are being developed as a means to reduce microbial colonization on the denture surface.

Purpose

The purpose of this study was to investigate the physical and mechanical properties of phosphate-containing polymethyl methacrylate resins for their suitability as a denture material.

Material and methods

Using PMMA with cross-linker (Lucitone 199) as a control, 4 experimental groups containing various levels of phosphate with and without cross-linker were generated. The properties examined …

Unusual Ectopic Eruption Of A Permanent Central Incisor Following An Intrusion Injury To The Primary Tooth, Ebru Canoglu, Cenk Ahmet Akcan, Erdinç Baharoglu, H. Cem Güngör, Zafer C. Cehreli

School of Dentistry Faculty Research and Publications

Intrusive luxation of primary teeth carries a high risk of damage to underlying permanent tooth germs. Ectopic eruption of permanent incisors is an unusual outcome of traumatic injury to their predecessors. In this case report, we describe the multidisciplinary management of the consequences of a primary tooth intrusion that led to severe ectopic eruption of the permanent left central incisor in a horizontal position at the level of the labial sulcus.

Prenatal Education: Priorities For Perinatal Nurses, Lisa Hanson

College of Nursing Faculty Research and Publications

No abstract provided.

Loneliness: A Concept Analysis, Abir K. Bekhet, Jaclene A. Zauszniewski

College of Nursing Faculty Research and Publications

TOPIC. Loneliness is a universal human experience recognized since the dawn of time, yet it is unique for every individual. Loneliness can lead to both depression and low self‐esteem.

PURPOSE. This article explicates the concept of loneliness through the examination of its conceptual definition and uses, defining attributes, related concepts, and empirical referents.

SOURCES OF INFORMATION. Literature review using hand search and database were used as sources of information.

CONCLUSION. Because loneliness is commonly encountered in nursing situations, the information provided will serve as a framework for assessment, planning, intervention, and evaluation of clients.

Variability In The Hormonally Estimated Fertile Phase Of The Menstrual Cycle, Richard Fehring, Mary Schneider

College of Nursing Faculty Research and Publications

The purpose of this study was to determine the variability in length of the fertile phase of the menstrual cycle with 140 participants who produced 1,060 cycles with an electronic hormonal fertility monitor. The length of the fertile phase, as defined by the first day with a threshold level of urinary E3G and ending with a second day above a threshold of LH, varied from7 days, with the most frequent length being 3 days.

Evidence That Α2-Antiplasmin Becomes Covalently Ligated To Plasma Fibrinogen In The Circulation: A New Role For Plasma Factor Xiii In Fibrinolysis Regulation, Michael W. Mosesson, Kevin R. Siebenlist, Irene Hernandez, K. N. Lee, V. J. Christiansen, P. A. Mckee

Biomedical Sciences Faculty Research and Publications

Background:

Plasma alpha₂-antiplasmin (α₂AP) is a rapid and effective inhibitor of the fibrinolytic enzyme plasmin. Congenital α₂AP deficiency results in a severe hemorrhagic disorder due to accelerated fibrinolysis. It is well established that in the presence of thrombin-activated factor XIII (FXIIIa), α₂AP becomes covalently ligated to the distal α chains of fibrin or fibrinogen at lysine 303 (two potential sites per molecule). Some time ago we showed that α₂AP is covalently linked to plasma fibrinogen . That singular observation led to our hypothesis that native plasma factor XIII (FXIII), …

The Dilemma Of The Open Gingival Embrasure Between Maxillary Central Incisors, Orlando Motohiro Tanaka, Bruno D'Aurea Furquim, Renata Correa Pascotto, Gerson Luiz Ulema Ribeiro, Jose A. Bosio, Hirushi Maruo

School of Dentistry Faculty Research and Publications

Aim: The aim of this report is to present the etiology, diagnosis, and treatment planning strategy in the presence of an open gingival embrasure between the maxillary central incisors.

Background: The open gingival embrasure or “black triangle” is a visible triangular space in the cervical region of the maxillary incisors. It appears when the gingival papilla does not completely fill in the interdental space. The space may occur due to: (1) disease or surgery with periodontal attachment loss resulting in gingival recession; (2) severely malaligned maxillary incisors; (3) divergent roots; or (4) triangular-shaped crowns associated with or without periodontal problems …

Resting Energy Expenditure And Delayed-Onset Muscle Soreness After Full-Body Resistance Training With An Eccentric Concentration, Kyle J. Hackney, Hermann-J. Engels, Randall J. Gretebeck

College of Nursing Faculty Research and Publications

The purpose of this investigation was to determine the effect of an acute bout of high-volume, full-body resistance training with an eccentric concentration on resting energy expenditure (REE) and indicators of delayed-onset muscle soreness (DOMS). Eight resistance trained (RT) and eight untrained (UT) participants (mean: age = 23.5 years; height = 180.76 cm; weight = 87.58 kg; body fat = 19.34%; lean mass = 68.71 kg) were measured on four consecutive mornings for REE and indicators of DOMS: creatine kinase (CK) and rating of perceived muscle soreness (RPMS). Delayed-onset muscle soreness was induced by performing eight exercises, eight sets, and …

Supporting A Community Of Individuals With Intellectual And Developmental Disabilities In Grieving, Sarah Ailey, Marilyn O'Rourke, Susan Breakwell, Anna Murphy

College of Nursing Faculty Research and Publications

Individuals with intellectual and developmental disabilities are living longer and experiencing deaths among their family and peers, yet their grieving is not well recognized. Staff members who provide care for these individuals witness the aging and death of their clients, yet they also receive little preparation to work through issues surrounding bereavement for their clients and themselves. The End of Life Nursing Education Consortium developed a national initiative to improve end-of-life and bereavement care education. Recommendations from the End of Life Nursing Education Consortium were incorporated into a home care/hospice/community health course and practicum for second-degree BSN students. The following …

Blunted Cystine–Glutamate Antiporter Function In The Nucleus Accumbens Promotes Cocaine-Induced Drug Seeking, Kristen S. Kau, Aric Madayag, John R. Mantsch, Mark D. Grier, Omer Abdulhameed, David A. Baker

Biomedical Sciences Faculty Research and Publications

Repeated cocaine alters glutamate neurotransmission, in part, by reducing cystine–glutamate exchange via system x_c−, which maintains glutamate levels and receptor stimulation in the extrasynaptic compartment. In the present study, we undertook two approaches to determine the significance of plasticity involving system x_c−. First, we examined whether the cysteine prodrug N-acetylcysteine attenuates cocaine-primed reinstatement by targeting system x_c−. Rats were trained to self-administer cocaine (1 mg/kg/200 μl, i.v.) under extended access conditions (6 h/day). After extinction training, cocaine (10 mg/kg, i.p.) primed reinstatement was assessed in rats pretreated with N-acetylcysteine (0–60 mg/kg, i.p.) in the …

Readiness For Discharge In Parents Of Hospitalized Children, Marianne E. Weiss, Norah Louise Johnson, Shelly Malin, Teresa A. Jerofke, Cecilia Lang, Eileen Sherburne

College of Nursing Faculty Research and Publications

Parental preparation for a child's discharge from the hospital sets the stage for successful transitioning to care and recovery at home. In this study of 135 parents of hospitalized children, the quality of discharge teaching, particularly the nurses' skills in “delivery” of parent teaching, was associated with increased parental readiness for discharge, which was associated with less coping difficulty during the first 3 weeks postdischarge. Parental coping difficulty was predictive of greater utilization of posthospitalization health services. These results validate the role of the skilled nurse as a teacher in promoting positive outcomes at discharge and beyond the hospitalization.

Patients' Perceptions Of Hospital Discharge Informational Content, Lynn Rose Maloney, Marianne Weiss

College of Nursing Faculty Research and Publications

Ensuring that patients' informational needs have been met prior to hospital discharge sets the stage for successful self-management of recovery at home. This secondary analysis study aims to identify differences in the amount of discharge teaching content needed and received by adult medical-surgical patients on the basis of their sociodemographic characteristics and hospitalization-related factors. The Quality of Discharge Teaching Scale (QDTS) is used to measure patients' perceptions of the amount of discharge-related informational content they needed and received. Eighty-nine percent of patients receive more informational content than they perceived they needed. Nonwhite patients report more content needed than White patients. …

Quantification Of The Individual Characteristics Of The Human Dentition: Methodology, L Thomas Johnson, Daniel D. Blinka, Peggy Jean Van Scotter-Asbach, Thomas W. Radmer

School of Dentistry Faculty Research and Publications

This study provides a method for comparing six individual human dentition characteristics using the standard measuring tool in Adobe Photoshop CS2 as compared to measuring individual characteristics with an automated software program under development at Marquette University, which has been adapted for bitemark analysis. The algorithm identifies color-specific pixels and automatically calculates the measurements.

Polymerization Efficiency Of Glass-Ionomer And Resin Adhesives Under Molar Bands, Daniel John Rejman, Theodore Eliades, T. Gerard Bradley, George Eliades

School of Dentistry Faculty Research and Publications

Objective: To determine the degree of cure of a light-cured resin-modified glass ionomer (RMGI) under molar bands compared with a light-cured resin and a dual-cured resin.

Materials and Methods: The 3 cements used were Fuji Ortho LC, Eagle Spectrum resin, and Variolink II dual-cure. Each sample was indirectly light cured for 20 seconds (10 seconds occlusally, 10 seconds cervically) under sections of molar bands, and the degree of cure was evaluated with micro-MIR FTIR spectroscopy.

Results: The RMGI exhibited a significantly higher mean degree of cure (55.31%) than both of the resins (Eagle 19.23%; Variolink II, 25.42%), which did not …

The Development Of The Napnap Research Agenda: Priorities For Evidence In Pediatric Practice 2008-2013, Kathleen J. Sawin, Arlene Butz, Margaret A. Brady, Agatha M. Gallo, Dolores Jones, Linda C. Lewin, Victoria P. Niederhauser, Christine A. Schindler, Cynthia A. Trent

College of Nursing Faculty Research and Publications

No abstract provided.

Refinement Of The Shared Care Instrument-Revised: A Measure Of A Family Care Interaction, Margaret Sebern

College of Nursing Faculty Research and Publications

This study’s purpose was to evaluate the psychometric properties of the Shared Care Instrument-Revised (SCI-R) in a sample of family care dyads. The SCI-R was developed to measure the construct of shared care, which is a system of three constructs (communication, decision making, reciprocity) used in family care to exchange support. An important aspect of evaluating the SCI-R was to create a measure that is statistically sound and meaningful for patient and caregivers. Surveys were mailed to randomly selected home health dyads, which included 223 patients and 220 caregivers. Reliability and confirmatory factor analysis, and concurrent validity were examined. Internal …

Factors Associated With Perceived Burden, Resourcefulness, And Quality Of Life In Female Family Members Of Adults With Serious Mental Illness, Jaclene Zauszniewski, Abir Bekhet, M. Jane Suresky

College of Nursing Faculty Research and Publications

BACKGROUND: Each year, 54 million American adults are affected by serious mental illness. Most of these persons depend on female family members for support or assistance, and unless these women are resourceful, they may experience considerable burden, stigma by association, depressive thoughts, and poor quality of life. OBJECTIVE:In this study, we examined the associations between characteristics of female family members (age, race, education), adults with serious mental illness (age, diagnosis), and the family situation (relationship, living arrangements, care provided) and caregivers' burden, stigma, depressive cognitions, resourcefulness, and quality of life.STUDY DESIGN:In this descriptive, cross-sectional study, a convenience …

Socio-Demographics Of Adult Orthodontic Visits In The United States, Joseph Whitesides, Nicholas M. Pajewski, T. Gerard Bradley, Anthony Iacopino, Christopher Okunseri

School of Dentistry Faculty Research and Publications

Introduction: Population-based studies in orthodontics have focused on differences between normative and perceived needs. However, information from national data on the prevalence of orthodontic visits and their associated factors in adults in the United States is scarce. We examined the demographic profile of likely adult users of orthodontic services and whether there is racial and ethnic disparity in orthodontic visits. Methods: We analyzed data from the Medical Expenditure Panel Survey, 2000-2004. Results: Overall, about 1% of the population reported an orthodontic visit. Subjects who made a general dental visit during the current year were significantly more likely to also have …

Histologic And Histomorphometric Analysis Of Posterior Region Of The Human Temporomandibular Disc, Luis Eduardo Almeida, Carla S. Baioni, Ana Paula C. Martins, Sergio Roberto P. Line, Lucia Noronha, Paula C. Trevilatto, Antonio Adilson S. De Lima, Marco Antonio De Oliveira Filho, Sergio Aparecido Ignacio

School of Dentistry Faculty Research and Publications

Objective

The aim of this study was to analyze histologic and histomorphometric features of the articular disc in groups with and without disc displacement.

Study design

A sample of 39 temporomandibular joints TMJs (31 case specimens, 8 control specimens) from 28 patients (mean age 31.2 years) were recruited for this study. The patients were considered to be affected and treated surgically with disc repositioning when presenting painful clinical signs of disc displacement after unsuccessful nonsurgical treatment for at least 6 months. Of the control patients, 4 presented condyle fracture which required opening to be reduced for treatment, and 4 displayed …

Activation Of Extracellular-Signal Regulated Kinase (Erk1/2) By Fluid Shear Is Ca2+- And Atp-Dependent In Mc3t3-E1 Osteoblasts, Dawei Liu, Damian C. Genetos, Ying Shao, Derik J. Geist, Jiliang Li, Hua Zhe Ke, Charles H. Turner, Randall L. Duncan

School of Dentistry Faculty Research and Publications

To determine the role of Ca²⁺ signaling in activation of the Mitogen-Activated Protein Kinase (MAPK) pathway, we subjected MC3T3-E1 pre-osteoblastic cells to inhibitors of Ca²⁺ signaling during application of fluid shear stress (FSS). FSS only activated ERK1/2, rapidly inducing phosphorylation within 5 min of the onset of shear. Phosphorylation of ERK1/2 (pERK1/2) was significantly reduced when Ca_i²⁺ was chelated with BAPTA or when Ca²⁺ was removed from the flow media. Inhibition of both the L-type voltage-sensitive Ca²⁺ channel and the mechanosensitive cation-selective channel blocked FSS-induced pERK1/2. Inhibition of phospholipase C with U73122 significantly reduced …

Functional Interactions Of Alcohol-Sensitive Sites In The N-Methyl-D-Aspartate Receptor M3 And M4 Domains, Hong Ren, Abdelghaffar K. Salous, Jaclyn M. Paul, Kaitlin A. Lamb, Donard S. Dwyer, Robert W. Peoples

Biomedical Sciences Faculty Research and Publications

The N-methyl-d-aspartate receptor is an important mediator of the behavioral effects of ethanol in the central nervous system. Previous studies have demonstrated sites in the third and fourth membrane-associated (M) domains of the N-methyl-d-aspartate receptor NR2A subunit that influence alcohol sensitivity and ion channel gating. We investigated whether two of these sites, Phe-637 in M3 and Met-823 in M4, interactively regulate the ethanol sensitivity of the receptor by testing dual substitution mutants at these positions. A majority of the mutations decreased steady-state glutamate EC₅₀ values and maximal steady-state to peak current ratios (I_ss/I …