Medicine and Health Sciences Commons^™

Regulation Of Rac1 Activation By The Low Density Lipoprotein Receptor–Related Protein, Zhong Ma, Keena S. Thomas, Donna J. Webb, Radim Moravec, Ana Maria Salicioni, Wendy M. Mars, Steven L. Gonias

Ana Maria Salicioni

The low density lipoprotein receptor–related protein (LRP-1) binds and mediates the endocytosis of multiple ligands, transports the urokinase-type plasminogen activator receptor (uPAR) and other membrane proteins into endosomes, and binds intracellular adaptor proteins involved in cell signaling. In this paper, we show that in murine embryonic fibroblasts (MEFs) and L929 cells, LRP-1 functions as a major regulator of Rac1 activation, and that this activity depends on uPAR. LRP-1–deficient MEFs demonstrated increased Rac1 activation compared with LRP-1–expressing MEFs, and this property was reversed by expressing the VLDL receptor, a member of the same gene family as LRP-1, with overlapping ligand-binding specificity. …

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Sumpun Thammacharoen

The Thai Journal of Veterinary Medicine

No abstract provided.

Pituitary Adenoma In A Horse: A Case Report, Nopadon Pirarat, Kohsaku Chijiwa, Pattama Ritruechai, Sawang Kesdangsakonwut, Komkrich Teankum, Wijit Banlunara, Anudep Rungsipipat, Boonmee Sunyasutjaree

The Thai Journal of Veterinary Medicine

A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, high fever, dysuria, nasal discharge and moist rale lung sounds. Gross examination revealed a single, white, firm, pituitary mass with a diameter 2.5 x 2.1 x 1.5 cm, compressed and overlying the hypothalamus. Histopathological and immunohistochemical examinations revealed the characteristic of a ACTH-derived pituitary adenoma.

A Retrospective Immunohistochemistry Study On Feline Panleukopenia Virus, Induced Enteritis, In Cats, Nopadon Pirarat, Theerayuth Kaewamatawong, Somporn Techangamsuwan

The Thai Journal of Veterinary Medicine

The retrospective study of 30 cases records using formalin-fixed, paraffin-embedded intestines, from Feline Panleukopenia (FPL) infected cats using histopathological criteria as seen by the Department of Pathology, Faculty of Veterinary Sciences, Chulalongkorn University between 1997-2001, was undertaken. The objective of the study was to examine FPLV infection in feline intestines showing characteristic lesions of parvovirus enteritis and to describe the relationship between the intestinal microscopic lesions of FPL in affected cats, with the location of FPL antigens, using immunohistochemical methods. The most striking histological lesions were villous atrophy and epithelial cell necrosis 96.67%. The lumen of crypts were often greatly …

The Invasion Of Guppy Skin By Tetrahymena Spp. After Induced Injuries, Aranya Ponpornpisit, Makoto Endo

The Thai Journal of Veterinary Medicine

A study was carried out on the invasion of Guppy skin by Tetrahymena spp. after various types of skin injury. Three types of skin injuries, scale removal, brush scratching and acetic acid irritation were performed. Tetrahymena spp. only the acetic acid irritation method allowed to invade the damaged skin. Guppy skin using this method, the fish caudal fin was covered with a 10%, acetic acid soaked, cotton strip for three minutes and exposed to water containing Tetrahymena spp. for 24 hours. Successful invasion used 100 Tetrahymena spp. cells/ml. in the water, at 25oC and a pH of 7.0-8.0. Histopathological examination …

Salmonella Spp., Coliforms And Total Bacterial Counts From Cultured Abalones, Perasut Pianpijit, Suksan Juhong, Nattakan Tun, Channarong Rodkum, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

The first commercial farm in Thailand to culture Abalone was studied to approve sanitation and food safety. Water quality and bacterial contamination criteria in both water from culturerelated sources and Abalone prior to marketing was examined. The water quality and bacteria found in the water samples (total aerobic plate, Vibrio count and MPN total coliforms) indicated the culturing practices adhere to the standards for shellfish sanitation. Total coliforms and other bacteria found in the Abalone samples also complied with microbiological guidelines for seafood products. The study details good farm manufacturing practices which can assure the food safety of Abalone. Further …

The Determination Of Serum Copper And Zinc Concentrations In Thoroughbred Foals And Their Dams After Parturition, Achara Tawatsin, Panumas Ruantongdee, Prachak Poomvises

The Thai Journal of Veterinary Medicine

Serum copper (Cu) and zinc (Zn) concentrations in 5-35 thoroughbred foals and their dams during first 24 weeks after parturition were determined. Mean serum Cu in foals increased significantly (p < 0.01) with age, from 10.4±1.1 μmol/l at week 1 to 25.9±0.8 μmol/l at week 24, whereas those in mares decreased significantly p < 0.05, from 26.2±1.3 μmol/l at week 1 to 22.0±0.5 μmol/l at week 24 after parturition. There was a large negative correlation between the mean serum Cu concentrations in foals and their dams (r = -0.86, p < 0.01). Serum Zn concentrations in both foals and mares on the other hand, gradually decreased after parturition, ranging from and 17.7±0.3 μmol/l at week-1 to 13.8±0.5 μmol/l at week 24, in foals and from 16.2±1.2 μmol/l at week 1 to 14.4±0.5 μmol/l at week 24 in mares. The correlation coefficient between the mean serum Zn concentrations in foals and their mares was high (r = 0.895, p < 0.01).

Treatment Of Chicken Caecal Coccidiosis With Sulfamonomethoxine In A Trial Conducted In A Battery House, Nopadon Pirarat, Autthapon Suntharathonpiphut, Kridtasak Sang-Gassanee, Anuchittada Sirisriro, Komkrich Teankum, Manop Muangyai

The Thai Journal of Veterinary Medicine

The treatment of chicken caecal coccidiosis with sulfamonomethoxine, at a concentration of 0.05 % in drinking water for 3 days, was performed on different days post oocyst inoculation.162 chickens were divided into 6 groups, 3 cages per group with 9 chicken each group. Chickens in groups 1-5 obtained 7x104 oocysts of Eimeria tenella, via a stomach tube. Chickens in group 1, 2 and 3 were treated on days 1-3, 2-4 and 3-5 post inoculation respectively. Group 4, a control group was infected but unmedicated. Group 5 was uninfected and unmedicated and group 6 was studied for drug toxicity observation. The …

Abalone Cultivation In Thailand: A Case Study, Nattakan Tun, Perasut Pianpijit, Suksan Juhong, Channarong Rodkum, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Abalone are high cost seafoods regarded as delicacy. The decline of world abalone fishery has initiated the development of cultured abalone production in some countries. Thailand possesses geographical areas which are suitable for abalone habitats and the commercial abalone farming is currently promoted by the government. Commercial fishery for abalone in Thailand are presently unfamiliarity of their biology. This report describes the basis biology of abalone, development of abalone culture in tropical regions; selection and management of broodstock, in tank spawning, rearing of larvae through grow-out culture of advanced juveniles, to meet the requirement of consumers' seafood standard.

Seminal Plasma: Its Function And Importance For Pig Artificial Insemination, Kampon Kaeoket, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The objective of this review is to update information concerning the function of seminal plasma on the pig reproductive system, as well as its importance in artificial insemination (AI). It has been shown that seminal plasma advances ovulation in gilts by an average time of 10-14 hours, improves sperm motility, sustaines sperm viability during uterine transport, suppresses immune activities (chemotaxis of neutrophils), improved sperm transport, improves fertilisation rate, improves litter size in gilts and indirectly enhances fertility. The hormone estrogen, within the seminal plasma, causes a release of prostaglandins from the pigs endometrium to the utero-ovarian veins and lymphatic vessels. …

Neuroanatomy Of The Common Dolphin (Delphinus Delphis) As Revealed By Magnetic Resonance Imaging (Mri), L. Marino, Keith Sudheimer, D. Ann Pabst, William A. Mclellan, David Filsoof, John I. Johnson

Veterinary Science and Medicine Collection

In this study, magnetic resonance (MR) images of the brain of an adult common dolphin (Delphinus delphis) were acquired in the coronal plane at 66 antero-posterior levels. From these scans a computer-generated set of resectioned virtual images in orthogonal planes was constructed using the programs VoxelView and VoxelMath (Vital Images, Inc., Michigan State Univ.). Sections in all three planes reveal major neuroanatomical structures. These structures in the adult common dolphin brain are compared with those from a fetal common dolphin brain from a previously published study as well as with MR images of adult brains of other odontocetes. …

Description Of A Poorly Differentiated Carcinoma Within The Brainstem Of A White Whale (Delphinapterus Leucas) From Magnetic Resonance Images And Histological Analysis, Sam H. Ridgway, Lori Marino, T. P. Lipscomb

Veterinary Science and Medicine Collection

In this study we used magnetic resonance imaging (MRI) to investigate neuroanatomical structure in the brain of a white whale (Delphinapterus leucas) that died from a large tumor within the brainstem. This specimen was also compared with a normal white whale brain using MRI. MRI scans of the white whale specimen show how the tumor deformed surrounding brain structure. Histopathological analysis indicated a poorly differentiated carcinoma of uncertain origin. These analyses demonstrate the usefulness of supplementing histological analyses of pathology with studies of gross morphology facilitated by MRI.

Thai Journal Of Veterinary Medicine Volume 32, 2002 Supplement, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

No abstract provided.

Modern Elements Of Informed Consent For General Veterinary Practitioners, Martin J. Fettman, Bernard Rollin

Professional Veterinary Ethics Collection

No abstract provided.

Endogenous Gonadal Hormone Exposure And Bone Sarcoma Risk, Dawn M. Cooley, Benjamin C. Beranek, Deborah L. Schlittler, Nita W. Glickman, Lawrence T. Glickman, David J. Waters

Endocrinology Collection

Although experimental and clinical evidence suggest that endogenous sex hormones influence bone sarcoma genesis, the hypothesis has not been adequately tested in an appropriate animal model. We conducted a historical cohort study of Rottweiler dogs because they frequently undergo elective gonadectomy and spontaneously develop appendicular bone sarcomas, which mimic the biological behavior of the osteosarcomas that affect children and adolescents. Data were collected by questionnaire from owners of 683 Rottweiler dogs living in North America. To determine whether there was an association between endogenous sex hormones and risk of bone sarcoma, relative risk (RR) of incidence rates and hazard ratios …

Passive Transfer Of Virus-Specific Antibodies Confers Protection Against Reproductive Failure Induced By A Virulent Strain Of Porcine Reproductive And Respiratory Syndrome Virus And Establishes Sterilizing Immunity, Fernando A. Osorio, J. A. Galeota, E. Nelson, Bruce W. Brodersen, Alan R. Doster, R. Wills, F. Zuckermann, W. W. Laegried

School of Veterinary and Biomedical Sciences: Faculty Publications

Immune mechanisms mediating protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) are not well understood. The PRRSV-specific humoral immune response has been dismissed as being ineffective and perhaps deleterious for the host. The function of PRRSV antibodies in protective immunity against infection with a highly abortifacient strain of this virus was examined by passive transfer experiments in pregnant swine. All of a group of pregnant gilts (n = 6) that received PRRSV immunoglobulin (Ig) from PRRSV-convalescent, hyperimmune animals were fully protected from reproductive failure as judged by 95% viability of offspring at weaning (15 days of age). …

Compounds Encoding The Protective M-Like Protein Of Streptococcus Equi And Assays Therefor, John F. Timoney, Sergey Artiushin

Veterinary Science Faculty Patents

The present invention relates generally to molecular compounds which encode the protective M-like protein of Streptococcus equi (SeM), the amino acid compound which is thereby encoded, and compositions of matter which incorporate either the encoding compounds or the cellular components for which they encode. For instance, vaccines which utilize the amino acid compounds or vectors and cell lines useful to make the amino acid compounds described herein are subjects of the present invention. The present invention provides methods to stimulate S. equi-specific immune response in horses. It also provides diagnostic assays for Streptococcus equi.

Short Communication: Relationship Between Body Growth And Mammary Development In Dairy Heifers, L F. Silva, M J. Vandehaar, Brian K. Whitlock, R P. Radcliff, H A. Tucker

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Our objective was to determine if prepubertal rate of body weight (BW) gain, independent of diet, was related to mammary development of dairy heifers. Data from two studies recently conducted at Michigan State University were used to identify factors, within a dietary treatment group, that would account for variation in first lactation milk production or amount of mammary parenchymal DNA at the time of puberty. Factors analyzed for variation in milk production during first lactation were: postpartum BW, prepubertal BW gain, gestational BW gain, postpartum BW gain, body condition score (BCS) at breeding, and BCS at calving. Factors analyzed for …

Short Communication: Relationship Between Body Growth And Mammary Development In Dairy Heifers, L F. Silva, M J. Vandehaar, Brian K. Whitlock, R P. Radcliff, H A. Tucker

Brian K Whitlock, PhD, DVM, DACT

Our objective was to determine if prepubertal rate of body weight (BW) gain, independent of diet, was related to mammary development of dairy heifers. Data from two studies recently conducted at Michigan State University were used to identify factors, within a dietary treatment group, that would account for variation in first lactation milk production or amount of mammary parenchymal DNA at the time of puberty. Factors analyzed for variation in milk production during first lactation were: postpartum BW, prepubertal BW gain, gestational BW gain, postpartum BW gain, body condition score (BCS) at breeding, and BCS at calving. Factors analyzed for …

Retinal Histopathology Of An Xlrp Carrier With A Mutation In The Rpgr Exon Orf15, Gustavo Aguirre, Beverly Yashar, Sinoj John, Julie Smith, K Breuer, S Hiriyanna, Anand Swaroop, Ann Milam

Gustavo D. Aguirre, VMD, PhD

Encapsulated Cell-Based Delivery Of Cntf Reduces Photoreceptor Degeneration In Animal Models Of Retinitis Pigmentosa, Weng Tao, Rong Wen, Moses B. Goddard, Sandy D. Sherman, Pam J. O'Rourke, Paul F. Stabila, William J. Bell, Brenda J. Dean, Konrad A. Kauper, Veronica A. Budz, William G. Tsiaras, Gregory M. Acland, Sue Pearce-Kelling, Alan Laties, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the …

Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Seasonal Effects On The Reproductive Performance Of Gilts And Sows, Padet Tummaruk, Wichai Tantasuparuk, Mongkol Techakumphu, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

Although the domestic pig is not a true seasonal breeder, a seasonal effect on reproductive performance exists. A reduction in reproductive performance during some periods of the year is most pronounced in primiparous sows and is manifested in various ways such as, delayed puberty, prolonged weaning-to-first-service interval (WSI), increased number of sows returning to oestrus after mating, decreased conception rate, decreased farrowing rate (FR), decreased litter size and an increased incidence of abortion. Both high ambient temperatures and/or changes in photoperiod have been reported to be the cause of these problems. The pig is considered to be a short-day seasonal …

A Modified Pelvic Fixation Technique Using Screws For The Treatment Of Hip Dysplasia In Dogs, Kiatpichet Komin, Marissak Kalpravidh, Atichat Brahmasa

The Thai Journal of Veterinary Medicine

Eight dysplastic hips of seven dogs suffering from mild hip dysplasia, as classified by the Orthopedic Foundation of Animals (OFA), were treated using a modified pelvic fixation technique. After pelvic, ischial and ilial osteotomies, the pelvis was plane-adjusted and fixed using screws to increase the acetabular coverage over the femoral head. Evaluation was made of the animals gait, by hip palpation and by taking OFA standard, ventrodorsal radiographs before and immediately after the surgical operation, followed by further evaluation 2, 4, 6, 8, 12, 16, 20 and 24 weeks later. The degree of acetabular coverage over the femoral head was …

Radiographic Evaluation Of Coxofemoral Joint Laxity In Dogs Part Ii: Comparison Of Stress - Radiographic Positioning Techniques In Dogs With Hip Dysplasia, Duangdaun Kaenkangploo, Phiwipha Kamonrat, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

Two stress-radiographic positioning techniques for evaluation of coxofemoral joint laxity in dogs with hip dysplasia were compared with the standard technique. Forty, healthy, large breed dogs were divided into two groups of 20 dogs. Group 1 had normal hips. Group 2 were dogs with mild to moderate grade of hip dysplasia according to the Orthopedic Foundation for Animals (OFA) standard. Dogs were anesthetized and placed in dorsal recumbency before 3 radiographic techniques, standard hip-extended, 60O and 90O stress techniques, were taken. For the 60O stress technique, hind legs were extended in parallel to each other at 60O to the table …

The Application Of A Rt-Pcr Technique For The Diagnosis Of Canine Distemper Using Peripheral Blood Mononuclear Cells, Weerawan Tiwananthakorn, Sirichai Junsuwanaruk, Achariya Sailasuta, Anudep Rungsipipat, Somporn Techangamsuwan, Sapasiri Siripornprasert, Susumu Tateyama, Ryoji Yamaguchi, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The application of a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) on 37 canine distemper cases was performed by the amplification of the nucleo-capsid gene derived from the canine distemper virus or CDV-infected mononuclear cells (PBMCs). The animals were divided into 2 groups according to their clinical signs. The first group of animals showed signs of distemper, such as respiratory and/or digestive signs. The second group showed the same signs as first group but with nervous involvements. The findings revealed that 74.1% (20/27) of group 1 tested positive with the RT-PCR assay compared to 60% (6/10) of group 2. These results suggested …