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Articles 1 - 30 of 291
Full-Text Articles in Medicine and Health Sciences
Extensive Conservation Of Sex Chromosome Organization Between Cat And Human Revealed By Parallel Radiation Hybrid Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Jill Pecon-Slattery, Stephen J. O'Brien
Extensive Conservation Of Sex Chromosome Organization Between Cat And Human Revealed By Parallel Radiation Hybrid Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Jill Pecon-Slattery, Stephen J. O'Brien
Biology Faculty Articles
A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human …
The Role Of Brn-3.2 In Retinal Ganglion Cell Differentiation, Jerin Marie Wright
The Role Of Brn-3.2 In Retinal Ganglion Cell Differentiation, Jerin Marie Wright
Loma Linda University Electronic Theses, Dissertations & Projects
Within the retina, the POU domain of transcription factors brn-3.0, brn-3.1, and brn-3.2 are present only in retinal ganglion cells. These genes are believed to be involved in establishing neural cell lineages in mammals. In this study brn-3.2 was examined by comparing the number of ganglion cells present during postnatal development in normal mice (+/+), in mice homozygous (-/-) for the brn-3.2 gene, and in adult mice with a heterozygous gene deletion (+/-) for brn-3.2. Optic nerve cross sections were imaged by electron microscopy, and axon profiles counted systematically by hand. These counts were then related to the nerve cross …
A Preclinical Model Of Ionizing Radiation And Tnf-Alpha Gene Therapy For Cancer, Jun Li
A Preclinical Model Of Ionizing Radiation And Tnf-Alpha Gene Therapy For Cancer, Jun Li
Loma Linda University Electronic Theses, Dissertations & Projects
Long term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. This study sought to determine if pretreatment with plasmid DNA expressing Tumor Necrosis Factor-α (TNF-α) could enhance the effect of ionizing radiation in an aggressive, rapidly growing glioma tumor (C6) model. Plasmid DNA is an attractive vector system for delivery of foreign genes into eukaryotic cells due to its advantages including low immunogenicity in vivo, lesser toxicity and relatively low cost. We have constructed a plasmid-based mammalian expression vector (pGL1-TNF) to secrete human TNF-α from cancer cells. Plasmid DNA was successfully transfected into C6 cells …
Protection From Oxidative Stress–Induced Apoptosis In Cortical Neuronal Cultures By Iron Chelators Is Associated With Enhanced Dna Binding Of Hypoxia-Inducible Factor-1 And Atf-1/Creb And Increased Expression Of Glycolytic Enzymes, P21waf1/Cip1, And Erythropoietin, Kevin O'Donovan
Kevin O'Donovan
No abstract provided.
The Egr Family Of Transcription-Regulatory Factors: Progress At The Interface Of Molecular And Systems Neuroscience, Kevin O'Donovan
The Egr Family Of Transcription-Regulatory Factors: Progress At The Interface Of Molecular And Systems Neuroscience, Kevin O'Donovan
Kevin O'Donovan
No abstract provided.
Method And Apparatus For Controlling Acoustic Signal Bandwidth In An Ultrasonic Diagnostic Imaging System, Jeffrey R. Resnick, Gregory R. Bashford
Method And Apparatus For Controlling Acoustic Signal Bandwidth In An Ultrasonic Diagnostic Imaging System, Jeffrey R. Resnick, Gregory R. Bashford
Biomedical Imaging and Biosignal Analysis Laboratory
An ultrasonic imaging system includes a receive beam former that generates analog receive signals and a scan converter. A receive signal processing path interconnects the receive beamformer and the scan converter, and this processing path includes both an A/D converter characterized by a selectable sampling rate and at least one filter characterized by at least one filter parameter. The filter parameter is selected as a function of the sampling rate to provide enhanced image quality.
Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran In A Forced Aeration System, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson
Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran In A Forced Aeration System, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson
Biosystems and Agricultural Engineering Faculty Publications
The effect of aeration on the production of xylanase by Trichoderma longibrachiatum on wheat bran in a solid-state fermentation (SSF) system has not been investigated. This study was conducted to investigate the interactive effects of aeration, initial moisture content of the substrate, and depth of the substrate on xylanase activity produced in a tray fermenter. The experiment was conducted as a split plot experiment with factorial treatments and three replications of each treatment combination. The whole plot treatment was aeration rate (0, 2.9, 5.7 L/min/kg bran). Initial moisture content (45, 55, 65% w.b.) and depth of substrate (1.0 and 2.5 …
Arthritis And Rosai-Dorfman Disease Of The Skin: A Diagnostic Dilemma, Irshad Nabi Soomro, Kamran Hameed
Arthritis And Rosai-Dorfman Disease Of The Skin: A Diagnostic Dilemma, Irshad Nabi Soomro, Kamran Hameed
Department of Pathology and Laboratory Medicine
No abstract provided.
Keto Pure Diet: Shark Tank Reviews (Side Effects, Ingredients) Cost …, Keto Pure Diet Shark Tank
Keto Pure Diet: Shark Tank Reviews (Side Effects, Ingredients) Cost …, Keto Pure Diet Shark Tank
Keto Pure Diet Shark Tank
Cartilage Regeneration On A Large Articular Surface Facilitated By Stress Shielding, Charles L. Mcdowell, Jennifer S. Wayne, Robert Tuten, Hunter H. Mcguire Jr.
Cartilage Regeneration On A Large Articular Surface Facilitated By Stress Shielding, Charles L. Mcdowell, Jennifer S. Wayne, Robert Tuten, Hunter H. Mcguire Jr.
Virginia Journal of Science
An animal model for the study of articular cartilage regeneration in-vivo facilitated by stress-shielding is introduced. The object of the model is to test the hypothesis that some form of cartilaginous tissue will grow upon a large joint surface in vivo with the joint in normal motion. The model utilizes the known capability of immature cells to differentiate. The source of cells is bleeding subchondral bone. In addition, the model provides a mechanically shielded environment in which cell differentiation and maturation can occur. The study showed that a substantial amount of tissue will grow in the animal model only when …
1999 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library
1999 Scholars And Artists Bibliography, Michael Schwartz Library, Cleveland State University, Friends Of The Michael Schwartz Library
Scholars and Artists Bibliographies
This bibliography was created for the annual Friends of the Michael Schwartz Library Scholars and Artists Reception, recognizing scholarly and creative achievements of Cleveland State University faculty, staff and emeriti
A Study On The Effects Of The N-Terminal Amino Acid Sequence On The Activation Of Human T-Cell Leukemia Virus Type 1 Protease, Hidayah Muhammad Kendall
A Study On The Effects Of The N-Terminal Amino Acid Sequence On The Activation Of Human T-Cell Leukemia Virus Type 1 Protease, Hidayah Muhammad Kendall
Chemistry & Biochemistry Theses & Dissertations
Human T-cell leukemia virus type 1 (HTL V-1) is dependent upon the enzymatic activity of its protease for maturation. Maturation of the protease is facilitated by cleavage of specific amino acid residues, followed by dimerization. The effects of the amino acid sequence located N-terminally to the cleavage site on the ability of the protease to become active were the focus of the current study. These amino acid sequences were contributed by the plasmid vector into which the protease gene was inserted.
Surface probability analyses (SPAs) of the vectors, as well as for native sequences which produce the mature protease and …
P53 And Pcna Expression In Benign, Atypical And Malignant Meningiomas, R Ahmed, I N. Soomro, S A. Aziz, S H. Hasan
P53 And Pcna Expression In Benign, Atypical And Malignant Meningiomas, R Ahmed, I N. Soomro, S A. Aziz, S H. Hasan
Department of Pathology and Laboratory Medicine
Objective: Alterations: p53 genes are turning out to be the most common genetic alterations in human cancers. Due to long half-life of mutated p53, its detection is possible by immunohistochemistry. Proliferating cell nuclear antigen (PCNA) is expressed by dividing cells, hence has been shown to correlate with prognosis. We have used monoclonal antibodies protein DO-7 (p53) and PC10 (PCNA) to see whether their expression correlates with histological grading in meningethelial tumour.
Material and Methods: a Twenty nine meningiomas (20 benign, 7 atypical and 2 malignant) were selected from the records of our laboratory. p53 and PCNA expression was sought by …
Spinal Cord Compression Caused By Metastatic Epithelial Myoepithelial Carcinoma Of The Parotid Gland, I N. Soomro, A S. Hussainy, K Chishti, M H. Pui, S A. Khan, R Ahmed, S H. Hasan
Spinal Cord Compression Caused By Metastatic Epithelial Myoepithelial Carcinoma Of The Parotid Gland, I N. Soomro, A S. Hussainy, K Chishti, M H. Pui, S A. Khan, R Ahmed, S H. Hasan
Department of Pathology and Laboratory Medicine
No abstract provided.
Community-Based Education: Health Promotion For Farmers At The Country Market, M. Susan Jones, Beverly Siegrist
Community-Based Education: Health Promotion For Farmers At The Country Market, M. Susan Jones, Beverly Siegrist
Nursing Faculty Publications
This article describes a unique approach used by baccalaureate nursing students, enrolled in a two-semester community health course, to address the priority of health concerns of agricultural workers in their own rural communities.
Phenotypic Expressions Of Ccr5-Δ32/Δ32 Homozygosity, Giang T. Nguyen, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna Dimichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien
Phenotypic Expressions Of Ccr5-Δ32/Δ32 Homozygosity, Giang T. Nguyen, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna Dimichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien
Biology Faculty Articles
Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1.
Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis.
Results: …
Regulation Of Connexin43 By Phosphorylation, Maithili M. Shah
Regulation Of Connexin43 By Phosphorylation, Maithili M. Shah
Loma Linda University Electronic Theses, Dissertations & Projects
Gap junctions play a crucial role in embryogenesis, especially of the heart where connexin43 is thought to be important for its formation as well as synchronized contraction. Several reports have shown that connexin43 protein is phosphorylated on serine residues in vitro and in vivo.
Studies performed in our laboratory have demonstrated that cell-to-cell communication in cells expressing connexin43 can be controlled rapidly and reversibly by microinjection of active protein kinases or phosphatases that target serine or threonine residues. Phosphorylation by cAMP-dependent protein kinase (PKA) seems to favor channel opening, whereas the effect of protein kinase C (PKC) on channel gating …
Neuronal And Metabolic Increases In Pedunculopontine Nucleus Of Parkinson's Rats, Jonathan Dennis Carlson
Neuronal And Metabolic Increases In Pedunculopontine Nucleus Of Parkinson's Rats, Jonathan Dennis Carlson
Loma Linda University Electronic Theses, Dissertations & Projects
The theoretical mechanisms underlying the symptoms of Parkinson’s disease are based on dysfunctional output from the basal ganglia projected to the cortex via the thalamus. However, the subthalamic nucleus (STN) and substantia nigra pars reticularis (SNr) as part of the basal ganglia, also project to the brainstem, in particular to the pedunculopontine nucleus (PPN). Both the STN and SNr have altered neuronal activity in the 6-hydroxydopamine (6-OHDA) Parkinson’s model rat. Thus, it is hypothesized that the PPN is altered in this model of Parkinson’s disease.
This hypothesis was examined by comparing the rates of glucose metabolism and spontaneous neuronal activity …
Signaling Mechanisms For Muscarinic Receptor-Mediated Coronary Vasoconstriction In Isolated Rat Hearts, Yi Zhang
Electronic Theses and Dissertations
The signaling mechanisms for muscarinic receptor-mediated vasoconstriction in coronary resistance arteries were studied in KCl-arrested isolated rat hearts perfused at a constant flow rate. The cholinergic agonists acetylcholine and bethanechol were given by bolus injection or constant infusion. The coronary vascular resistance was monitored by measuring the changes in perfusion pressure. The selective muscarinic agonist bethanechol caused a similar vasoconstrictor response as ACh, but with less potency and efficacy. Bolus injection of bethanechol evoked a phasic vasoconstriction in a dose-dependent manner, while infusion of bethanechol evoked a tonic vasoconstriction without producing tachyphylaxis. Coronary vascular responses to bethanechol were further examined …
Effects Of Plasma Hiv Rna, Cd4+ T Lymphocytes, And The Chemokine Receptors Ccr5 And Ccr2b On Hiv Disease Progression In Hemophiliacs, Eric S. Daar, Sharyne Donfield, Edward Gomperts, Margaret Hilgartner, Keith Hoots, David Chernoff, Cheryl Winkler, Stephen J. O'Brien, Hemophilia Growth And Development Study
Effects Of Plasma Hiv Rna, Cd4+ T Lymphocytes, And The Chemokine Receptors Ccr5 And Ccr2b On Hiv Disease Progression In Hemophiliacs, Eric S. Daar, Sharyne Donfield, Edward Gomperts, Margaret Hilgartner, Keith Hoots, David Chernoff, Cheryl Winkler, Stephen J. O'Brien, Hemophilia Growth And Development Study
Biology Faculty Articles
We have investigated the effects of plasma HIV RNA, CD4+ T lymphocytes and chemokine receptors CCR5 and CCR2b on HIV disease progression in hemophiliacs. We prospectively observed during follow-up 207 HIV-infected hemophiliacs in the Hemophilia Growth and Development Study. Plasma HIV RNA was measured on cryopreserved plasma from enrollment using the Chiron Corporation bDNA (version 2.0) assay. Genotype variants CCR2b-641 and CCR5-Δ32 were detected using standard molecular techniques. Those with the mutant allele for CCR2b, and to a lesser extent CCR5, had lower plasma HIV RNA, and higher CD4+ T lymphocytes than did those without these …
Santa Clara Magazine, Volume 41 Number 2, August 1999, Santa Clara University
Santa Clara Magazine, Volume 41 Number 2, August 1999, Santa Clara University
Santa Clara Magazine
8 - APOCALYPSE PAST: Y1K By Thomas Turley. The year 2000 is upon us. But what about Y1K? History Professor Thomas Turley takes an historical look at the millennial fever of a thousand years ago.
12 - THE FUTURE IS MATH By Susan Vogel. Math Lecturer David Sul is on a mission: to get more Hispanics and African-Americans into Calculus and onto college through SCU's Calculus 100 program.
16 - The Class of 1999 Moves On By Sug Ramire '98. Optimism is the byword for the last graduating class of the millennium. Six 1999ers share their impressions, hopes, and fears …
Optimal Replication Activity Of Vesicular Stomatitis Virus Rna Polymerase Requires Phosphorylation Of A Residue(S) At Carboxy-Terminal Domain Ii Of Its Accessory Subunit, Phosphoprotein P, Leroy N. Hwang, Nathan Englund, Tapas Das, Amiya K. Florida, Asit K. Pattnaik
Optimal Replication Activity Of Vesicular Stomatitis Virus Rna Polymerase Requires Phosphorylation Of A Residue(S) At Carboxy-Terminal Domain Ii Of Its Accessory Subunit, Phosphoprotein P, Leroy N. Hwang, Nathan Englund, Tapas Das, Amiya K. Florida, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein must be phosphorylated for in vivo transcription activity, but not for replication activity, of the polymerase complex. To examine the role of phosphorylation of the carboxy-terminal domain II residues of the P protein in transcription and replication, we have used a panel of mutant P proteins in which the phosphate acceptor sites …
A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski
A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski
Dartmouth Scholarship
We describe here a new member of the LysR family of transcriptional regulators, AphB, which is required for activation of the Vibrio cholerae ToxR virulence cascade. AphB activates the transcription of the tcpPH operon in response to environmental stimuli, and this process requires cooperation with a second protein, AphA. The expression of neither aphA or aphB is strongly regulated by environmental stimuli, raising the possibility that the activities of the proteins themselves may be influenced under various conditions. Strains of the El Tor biotype of V. cholerae typically exhibit lower expression of ToxR-regulated virulence genes in vitro than classical strains …
Identification And Characterization Of Determinants Of Head And Neck Tumor Cell Invasion, Yangguan Wu
Identification And Characterization Of Determinants Of Head And Neck Tumor Cell Invasion, Yangguan Wu
Theses and Dissertations in Biomedical Sciences
Head and neck squamous cell carcinoma (HNSCC) is a common malignant disease with poor prognosis. The majority of patients die from local invasion or lymphatic metastasis. The mechanism(s) underlining the invasiveness of HNSCC are poorly understood. Utilizing a panel of HNSCC cell lines previously established in our laboratory, we tested the application and relevance of the three-step hypothesis of tumor invasion to HNSCC and investigated the mechanism(s) pertaining to the regulation of each step in the invasive process. Data presented in this thesis demonstrated that tumor cell invasion in HNSCC is a complex process involving three repeated sequential steps: adhesion, …
An Extracellular Protein Produced By Staphylococcus Hominis Strain Αm With Antimicrobial Activity Against Mycobacterium Spp, Sandra May Jacobsen
An Extracellular Protein Produced By Staphylococcus Hominis Strain Αm With Antimicrobial Activity Against Mycobacterium Spp, Sandra May Jacobsen
Biological Sciences Theses & Dissertations
The inhibitory activity exhibited by Staphylococcus hominis strain αM toward Mycobacterium species was examined. These studies included determining the conditions that maximized inhibitory agent production, analyzing characteristics of the agent, determining the time of agent production, ascertaining plasmid possession and the role of plasmids in the production of this inhibitory agent, and ascertaining the antimicrobial activity of other strains of S. hominis. On solid and in liquid media, S. hominis strain αM exhibits a unique inhibitory activity toward Mycobacterium species that is not demonstrated by Staphylococcus epidermidis or other S. hominis strains. Maximum agent production and recovery was …
Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.
Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.
Seton Hall University Dissertations and Theses (ETDs)
Antiphospholipid Syndrome (APS) is an evolving autoimmune disease with numerous clinical manifestations. APS occurs in two forms: Primary Antiphospholipid Syndrome (PAPS) and secondarily in association with other autoimmune disorders such as Systemic Lupus Erythematosis (SLE). In the brief period since its discovery as a cofactor for anticardiolipin antibodies, p1-glycoprotein I (�GPI; apolipoprotein H) has been recognized as the autoantigen in the absence of anionic phospholipid when appropriately presented to human autoantibodies. Recent studies suggest that this presentation requires the surface-dependent unmasking of a cryptic epitope. Immunoassays of autoantibodies directed against p1GPI, developed through the application of suitable polymeric matrices, may …
Generation Of Human Chorionic Gonadotropin Beta Subunit Specific Monoclonal Antibody Using A Synthetic Peptide, Zhe Huang
Chemistry & Biochemistry Theses & Dissertations
Human chorionic gonadotropin (hCG) is well known for early immunological and bioassay tests for early detection of pregnancy. Since the hormone specific beta subunit confers unique biological and immunological specificity to hCG, immunochemical measurement of hCG has achieved greater specificity and sensitivity in recent years by using either the beta subunit as an immunogen or a partially reduced beta subunit polypeptide chain. However, antiserum raised against the entire hCG beta subunit usually cross-reacts to some extent with human luteinizing hormone (hLH). hCG has been found to be present in the extract of tumors and minute quantities of hCG are synthesized …
Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof.
Pr. Mamoudou H. DICKO, PhD
The objective of this study was the research of novel and inexpensive sources of polysaccharides degrading enzymes such as amylases and glucanases from local plants in order to justify their biotechnological applications. The isolation of two l3-amylases and an endo-1,3-ß-D-glucanase was reached using common protein purification methods such as buffer extraction, ammonium sulfate fractionation, ionexchange and gel filtration chromatographies. The methods used were simple and easily reproducible, suggesting the possibilfty of large-scale production. ln the crude extract of Curculigo pilosa tuber, only ß-amylase was detected as starch degrading enzyme and its activity was approximately 282 Uig of fresh material. The …
Media Coverage Of Epa's Draft Dioxin Reassessment Report, Sharon M. Friedman, Megan A. Fitzpatrick, Brenda P. Egolf
Media Coverage Of Epa's Draft Dioxin Reassessment Report, Sharon M. Friedman, Megan A. Fitzpatrick, Brenda P. Egolf
RISK: Health, Safety & Environment (1990-2002)
Using content analysis, the authors examine the utility of news media in democratic decision making.
Morphological Pattern And Frequency Of Thyroid Tumors, S H. Shah, S Muzaffar, I N. Soomro, Sheema H. Hasan
Morphological Pattern And Frequency Of Thyroid Tumors, S H. Shah, S Muzaffar, I N. Soomro, Sheema H. Hasan
Department of Pathology and Laboratory Medicine
Objective:
The present study was done to evaluate the frequency of thyroid cancer and to find out the prevalence of histological types of thyroid tumor with respect to age and sex group.
Setting:
This study included consecutive cases of malignant tumors of thyroid gland, which were diagnosed in the Department of Pathology at the Aga Khan University Hospital, Karachi during the period of three years (1995-1997).
Methods:
These cases were evaluated on H & E stained sections from paraffin embedded 10% buffered formalin fixed tissue blocks. Special stains and immunohistochemical analysis were performed whenever required.Results: A total of 8541 malignant …