Medicine and Health Sciences Commons^™

Otoacoustic Emission In A Dog, Michael Sims, James Brace, D. Arthur, Ralph Harvey

James J Brace

No abstract provided.

Otoacoustic Emission In A Dog, Michael Sims, James Brace, D. Arthur, Ralph Harvey

Michael H. Sims

No abstract provided.

Evaluation Of Use Of Dimethyl Sulfoxide For Intra-Articular Lavage In Clinically Normal Horses, H Adair, Dallas Goble, S Vanhooser, James Blackford, Barton Rohrbach

James T. Blackford DVM, MS, DACVS

The antebrachiocarpal and tarsocrural joints of 10 adult horses were randomly assigned to 1 of 4 groups. Groups were formulated and were treated as follows: group 1, control (arthrocentesis only); group 2, buffered lactated Ringer solution; group 3, 10% dimethyl sulfoxide (DMSO; w/v) in lactated Ringer solution; and group 4, 30% DMSO (w/v) in lactated Ringer solution. Joints were lavaged once with the respective solution. Prior to lavage and on days 1, 4 and 8 after lavage, all horses were evaluated for lameness and joint effusion; synovial fluid total and differential WBC counts, synovial fluid total protein concentration ...

Comparison Of The Efficacy Of Three Commercial Feline Leukemia Virus Vaccines In A Natural Challenge Exposure, Alfred Legendre, D Hawks, R Sebring, Barton Rohrbach, L Chavez, H Chu, W Acree

Alfred M Legendre DVM, MS, DACVIM

Forty-seven kittens were exposed for 31 weeks to 12 FeLV-positive carrier cats. The carrier cats were infected with 2 laboratory strains of FeLV and at least 2 strains of street virus. Eleven nonvaccinated control kittens and 12 vaccinated kittens were allotted to 3 groups. After 31 weeks of exposure, the following kittens were persistently blood FeLV positive by ELISA and immunofluorescence antibody (IFA) testing: 7 of the 11 control kittens, 0 of 12 kittens inoculated with vaccine A, 5 of 12 kittens inoculated with vaccine B, and 6 of 12 kittens inoculated with vaccine C. Only the kittens inoculated with ...

Comparison Of Four Test Kits For Feline Leukemia Virus Antigen, D Hawks, Alfred Legendre, Barton Rohrbach

Alfred M Legendre DVM, MS, DACVIM

The sensitivity and specificity of 4 commercial FeLV ELISA kits, using blood were compared with results of virus isolation from blood and immunofluorescent antibody (IFA) testing on blood. Significant differences were not found among the 4 ELISA kits. Marked decrease in sensitivity of the ELISA kits was detected when virus isolation was used as the standard positive test rather than the IFA test. Virus isolation was a more sensitive indicator of early infection, with marked discrepancy among results obtained by virus isolation, ELISA, and the IFA test. Results became progressively more concordant as infection became fully established. Cats FeLV-positive by ...

Antibody Response Of Kittens After Vaccination Followed By Exposure To Feline Leukemia Virus-Infected Cats, D Hawks, Alfred Legendre, Barton Rohrbach, R Sebring, L Chavez, H Chu, W Acree

Alfred M Legendre DVM, MS, DACVIM

Protein (western) blot analysis and virus-neutralization assay were used to evaluate the antibody response of SPF kittens to FeLV vaccination and followed by natural exposure. Several kittens had barely detectable reactions to specific FeLV antigens prior to vaccination or exposure. Correlation was not found between protection against persistent viraemia and antibody response after vaccination as measured by western blot analysis or virus neutralization assay. A significant difference in the antibody response against p27 antigen after natural exposure to FeLV was observed between persistently viraemic kittens and transiently viraemic or aviraemic kittens. Measurable virus neutralizing antibody titre after FeLV exposure was ...

Texas D.O. Volume 48, Number 11, Texas Osteopathic Medical Association

Texas Osteopathic Physicians Journal

No abstract provided.

Uwomj Volume 61, Number 1, December 1991, Western University

University of Western Ontario Medical Journal

Schulich School of Medicine & Dentistry

Update - December 1991, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- The Physician as an Agent of God: An Idea Whose Time Has Come

-- [ Medical Futility: A Value-Dependent Concept ]

Time-Courses In The Retention Of Food Material In The Bivalves Potamocorbula Amurensis And Macoma Balthica: Significance To The Absorption Of Carbon And Chromium, Alan W. Decho, Samuel N. Luoma

Faculty Publications

Time courses for ingestion, retention and release via feces of microbial food was investigated using 2 bivalves with different feeding strategies, Potamocorbula amurensis, and Macoma balthica. The results showed 2 pathways for the uptake of food material in these clams. The first is represented by an initial label pulse in the feces. The second pathway operates over longer time periods. Inert ⁵¹Cr-labeled beads were used to determine time frames for these pathways. The first pathway, involving extracellular digestion and intestinal uptake, is relatively inefficient in the digestion of bacterial cells by P. amurensis but more efficient in M. balthica ...

Expression And Function Of Zebra: The Epstein-Barr Viral Replication Activator, Naomi Taylor

Yale Medicine Thesis Digital Library

Epstein-Barr virus (EBV) encodes a protein, ZEBRA, which enables the virus to switch from a latent to a lytic life cycle. ZEBRA expression was studied in lymphoid cells harboring either standard virus or a mixture of standard and defective viruses. ZEBRA protein could not be detected in cells latently infected with standard EBV but was constitutively expressed in cells containing both defective and standard EBV genomes. Experiments indicated that regulation of transcription of the ZEBRA gene (BZLF1) is a pivotal event in the control of EBV replication. ZEBRA specific BZLF1 transcripts were spontaneously expressed in cells harboring defective virus but ...

Preliminary Epidemiological Interpretation Of The Intrinsic And Extrinsic Factors Contributing To The Coverage And Participation Rates Of The Vaccination Program In Arssi, Ethiopia, Muchie Kidanu

Electronic Theses and Dissertations

Many immunization programs in developing countries arr now undergoing a period of rapid utilization. Innovative strategies, such as immunization holidays, are being tried. Political commitment and enthusiasm are high. There is a strong desire by health professionals to protect the lives of many children from the untimely death and/or disability resulting from infectious diseases preventable by vaccination.

Evaluation of an immunization program can benefit other elements of the health system, particularly those that are not amenable to objective evaluation as immunization. In this case, immunization can serve as an indicator of the overall performance of the health system. The ...

Supervised Training Of Practical Procedures In The Internal Medicine Residency, Z Abbas, S Abid, J A. Khan, A Jabbar

Section of Gastroenterology

In order to get feedback whether the medical residents are getting adequate supervised training to perform practical procedures, a questionnaire was distributed among the residents asking their involvement in sixteen frequently performed procedures in the medical unit of the Aga Khan University Medical Centre. Though the residents were satisfied about their training in doing lumbar punctures, pleural and peritoneal aspirations, bone marrow aspiration and trephine and chest tubes placement, they showed their concern about training in putting central lines, venous cut downs, cardiac pacing and biopsies. A better schedule training programme, organized subspecialty rotation, continuous evaluation of resident's skill ...

Comparison Of A Quantitative Microtiter Method, A Quantitative Automated Method, And The Plate-Count Method For Determining Microbial Complement Resistance, Margie D. Lee, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan, Emmett B. Shotts Jr.

Lisa K. Nolan

A quantitative microtiter method for determining the degree of complement resistance or sensitivity of microorganisms is described. The microtiter method is compared with a quantitative automated system and the standard plate-count technique. Data were accumulated from 30 avian Escherichia coli isolates incubated at 35 C with either chicken plasma or heat-inactivated chicken plasma. Analysis of data generated by the automated system and plate-count techniques resulted in a classification of the microorganisms into three groups: those sensitive to the action of complement; those of intermediate sensitivity to the action of complement; and those resistant to the action of complement. Although the ...

Comparison Of Chicken Plasma And Guinea Pig Serum In A Quantitative Microtiter Method Of Determining Microbial Complement Resistance, Richard E. Wooley, John Brown, Kathy R. Spears, Lisa K. Nolan

Lisa K. Nolan

Y. A quantitative microtiter method using chicken plasma is described for determining the degree of complement resistance or sensitivity of avian Escherichia coli isolates. Results obtained with the microtiter method using chicken plasma were compared with results obtained using commercially available standardized guinea pig serum as the source of complement. The test organisms consisted of five isolates of E. coli isolated from chickens. Three isolates were from flocks with colisepticemia; one was from a flock with omphalitis; and one isolate was a non-pathogenic control. Data were accumulated from the five avian E. coli isolates incubated at 35 C with either ...

Eosinophilic Gastroenteritis, S H. Shah, Z Abbas, S Abid, H Shaikh, T Qaseem, H Khan

Section of Gastroenterology

No abstract provided.

The Effects Of Two Types Of Hypnotic Suggestions On Analgesic Responding In Moderately Hypnotizable Subjects, Gloria M. Haddad

Master's Theses

This study assessed the effects of two hypnotic suggestions on hypnotic performance in medium hypnotizable subjects. Subjects were exposed at separate intervals to either an hypnotic suggestion patter containing specific imagery or alternatively to a suggestion patter that provided only general problem-solving direction. Hypnotic performance was assessed using tolerance and threshold measures on a cold-pressor test.

The problem-solving suggestions were shown to increase both threshhold and tolerance of noxious stimulation. The imagery suggestions proved to be effective only for the threshhold measure.

These findings are discussed in terms of their implications for the use of hypnosis with subjects who do ...

A Novel Transposon Trap For Mycobacteria: Isolation And Characterization Of Is1096, Jeffrey D. Cirillo, Raul G. Barletta, Barry R. Bloom, William R. Jacobs Jr.

Papers in Veterinary and Biomedical Science

In the course of developing strategies to obtain a mutation in the aspartate semialdehyde dehydrogenase (asd) gene of Mycobacterium smegmatis, an efficient transposon trap was constructed which may be generally useful for the identification of transposable elements in mycobacteria. A DNA fragment containing the asd gene was replaced with an aminoglycoside phosphotransferase gene (aph) to generate a Δasd::aph allele. Attempts to replace the wild-type asd gene with the Δasd::aph allele were unsuccessful, suggesting that this deletion was lethal to the growth of M. smegmatis. The plasmid, pYUB215, which contains β-galactosidase expressed from a mycobacteriophage promoter and ...

The Ability Of Simian Virus 40 Large T Antigen To Immortalize Primary Mouse Embryo Fibroblasts Cosegregates With Its Ability To Bind To P53., Jiyue Y. Zhu, Marina Abate, Philip W. Rice, Charles N. Cole

Open Dartmouth: Faculty Open Access Articles

The large T antigen encoded by simian virus 40 (SV40) plays essential roles in the infection of permissive cells, leading to production of progeny virions, and in the infection of nonpermissive cells, leading to malignant transformation. Primary mouse embryo fibroblasts (MEFs) are nonpermissive for SV40, and infection by wild-type SV40 leads to immortalization and transformation of a small percentage of infected cells. We examined the ability of an extensive set of mutants whose lesions affect SV40 large T antigen to immortalize MEFs. We found that immortalization activity was retained by all mutants whose lesions are located upstream of codon 346 ...

Expression Of Collagenlike Sequences By A Tumor Virus, Herpesvirus Saimiri, Peter Geck, Scott A. Whitaker, Maria M. Medveczky, Peter G. Medveczky

Open Access Articles

No abstract provided.

Glucose Transporter Oligomeric Structure Determines The Mechanism Of Glucose Transport: A Dissertation, Daniel N. Hebert

GSBS Dissertations and Theses

The relationship between human erythrocyte glucose transporter (GLUT1) oligomeric structure and function was studied. GLUT1 was purified from human erythrocytes in the absence (GLUT1-DTT) or the presence (GLUT1+DTT) of dithiothreitol. Chemical cross-linking studies of lipid bilayer-resident purified GLUT1 and hydrodynamic studies of cholate-solubilized GLUT1 support the view that GLUT1-DTT is a homotetramer and GLUT1+DTT is a homodimer. Parallel studies on human erythrocyte, and studies employing conformation-specific antibodies (anti-GLUT1-DTT antibodies, ∂-IgGs), indicate that erythrocyte-resident GLUT1 resembles GLUT1-DTT (a homotetramer). While the D-glucose binding capacities of GLUT1-DTT and GLUT1+DTT are indistinguishable, GLUT1-DTT presents at least two population of binding ...

The Relationships Between Marital Adjustment, Family Functioning, Task Management And Family Relationship Concerns In Couples Incorporating A Second Child, Amy Diguiseppe Bade

Dissertations

The purpose of this study was to examine the relationships among variables identified as potentially significant during the transition to second time parenthood. The theoretical framework for this study was based on the Circumplex Model of family systems (Olson, Sprenkle & Russell, 1979; Olson, Russell & Sprenkle, 1983), and a five domain structural model of marital and family adaptation (Cowan & Cowan, 1988).

The convenience sample consisted of 49 Caucasian middle class couples all expecting their second child. This was a one group pretest-posttest design with the birth of the second child functioning as the independent variable. The dependent variables examined in a longitudinal and ...

Discrete Nuclear Domains Of Poly(A) Rna And Their Relationship To The Functional Organization Of The Nucleus, Kenneth C. Carter, Krishan L. Taneja, Jeanne B. Lawrence

Open Access Articles

The functional organization of the nucleus was studied using a fluorescence microscopy approach which allowed integration of positional information for RNA, DNA, and proteins. In cells from sea urchin to human, nuclear poly(A) RNA was found concentrated primarily within several discrete "transcript domains" which often surrounded nucleoli. Concentrations of poly(A) RNA were coincident with snRNP antigen clusters, providing evidence for the localization of pre-mRNA splicing at these sites. The spatial relationship of transcript domains with respect to various classes of DNA was established, in that the poly(A) RNA-rich regions coincided with discrete regions of low DNA density ...

On Good Mothers And Good Managers, Genevieve Chandler

Genevieve E. Chandler

No abstract provided.

Reconstruction And Future Trends Of The Aids Epidemic In The United States, Ron Brookmeyer

Ron Brookmeyer

There has been considerable uncertainty in estimates of past and current human immunodeficiency virus (HIV) infection rates in the United States. Statistical estimates of historical infection rates can be obtained from acquired immunodeficiency syndrome (AIDS) incidence data and the incubation period. However, this approach is subject to a number of sources of uncertainty and two other approaches, epidemic models of HIV transmission and surveys of HIV prevalence, are used to corroborate and refine the statistical estimates. Analyses suggest the HIV infection rate in the United States grew rapidly in the early 1980s, peaked in the mid-1980s, and subsequently declined markedly ...

Representation Requirements For Supporting Knowledge-Based Construction Of Decision-Models In Medicine, Tze-Yun Leong

Research Collection School Of Information Systems

This paper analyzes the medical knowledge required for formulating decision models in the domain of pulmonary infectious diseases (PIDs) with acquired immunodeficiency syndrome (AIDS). Aiming to support dynamic decision-modeling, the knowledge characterization focuses on the ontology of the clinical decision problem. Relevant inference patterns and knowledge types are identified.

Apakah Kencing Nanah Akan Sembuh Sendiri Tanpa Bantuan Obat, Agen Resmi De Nature

agen resmi de nature

Perceptions Of Job Worth, Trinidad Argüelles

FIU Electronic Theses and Dissertations

The present study was conducted to evaluate perceptions of the importance of various factors that may determine the wage or salary level in jobs. Items describing various job characteristics reflecting the factors of Skill, Effort, Responsibility, Working Conditions, and Organizational characteristics were rated by 510 subjects from a variety of organizations. Results indicated that the items did not cluster into the five categories noted above. Instead, three factors were identified and labeled Job Complexity, Accountability, and Work Context. There were few gender or occupational differences in the ratings of the items. The implications of the results for the development of ...

Isolation And Expression Of A Gene Cluster Responsible For Biosynthesis Of The Glycopeptidolipid Antigens Of Micobacterium Avium, John T. Belisle, Lisa Pascopella, Julia M. Inamine, Patrick J. Brennan, William R. Jacobs Jr.

Public and Community Health Sciences Faculty Publications

Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are distinguished from all other mycobacteria and from one another by the presence of highly antigenic glycolipids, the glycopeptidolipids, on their surfaces. A genomic library of DNA from serovar 2 of the M. avium complex was constructed in the Escherichia coli-Mycobacterium shuttle cosmid, pYUB18, and used to clone and express in Mycobacterium smegmatis the genes responsible for the biosynthesis of the oligosaccharide segment of the M. avium serovar 2-specific glycopeptidolipid. The ...

Texas D.O. Volume 48, Number 10, Texas Osteopathic Medical Association

Texas Osteopathic Physicians Journal

No abstract provided.