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Full-Text Articles in Medicine and Health Sciences
Calcium/Diacylglycerol-Dependent Protein Phosphorylation In The Nervous System, Katherine Ann Albert
Calcium/Diacylglycerol-Dependent Protein Phosphorylation In The Nervous System, Katherine Ann Albert
Yale Medicine Thesis Digital Library
Calcium/diacylglycerol-dependent protein kinase, most often referred to as protein kinase C, is of interest to research groups across many scientific disciplines for two major reasons. First, protein kinase C is of general importance in signal transduction because its second messenger activator, diacylglycerol, is generated by phosphoinositide metabolism in response to a host of biologically active molecules. Second, protein kinase C has been identified as the cellular receptor for tumor-promoting phorbol esters. Protein kinase C is of particular interest to neuroscientists because its highest activity is in the nervous system. The studies described in this dissertation were undertaken to analyze the …
Interferon Action: A. Studies Of The Effect Of Interferons On Sv40 Replication. B. Studies Of The 2'-5' A System, Mariano Agustín García-Blanco
Interferon Action: A. Studies Of The Effect Of Interferons On Sv40 Replication. B. Studies Of The 2'-5' A System, Mariano Agustín García-Blanco
Yale Medicine Thesis Digital Library
This thesis is divided into two parts: the first deals with studies that focus on the effect of interferons on the expression of foreign genetic material. The second part deals with the interferon-regulated (2'5')oligoadenylate system. This system is most probably an effector arm of the anti-viral anti-mitogenic actions of interferons.I show here that interferons inhibit the expression of both early SV40 RNA and SV40 Tag, and SV40 DNA replication in SV40 infected cells. Interferons selectively inhibit the expression of the Tag encoded by superinfecting SV40 virions in SV40-transformed cells superinfected with SV40. The effect of interferon was seen when cells …
Identification, Characterization, And Purification Of A 65,000 Dalton Protein In Rat Brain That Is Photolabeled By Nitro-Containing Benzodiazepines, Allen Clarke Bowling
Identification, Characterization, And Purification Of A 65,000 Dalton Protein In Rat Brain That Is Photolabeled By Nitro-Containing Benzodiazepines, Allen Clarke Bowling
Yale Medicine Thesis Digital Library
Benzodiazepines bind to two well-characterized classes of nanomolar-affinity binding sites, the central and the peripheral types. Although these sites appear to mediate many of the effects of these compounds, they cannot account for all of the biochemical and physiologic effects of the benzodiazepines. In this investigation, a protein that is photolabeled by NO2-containing benzodiazepines was identified and characterized in rat brain by performing photaffinity labeling experiments with [3H] -clonazepam and [3H] -flunitrazepam. These experiments demonstrate that this photolabeled protein has a molecular weight of 65,000 daltons. Photolabeling of the protein was saturable, inhibited in a stereoselective manner by benzodiazepine enantiomers, …
Nonsense Mutations In The Human Beta Globin Gene Affect Mrna Metabolism, Susan J. Baserga
Nonsense Mutations In The Human Beta Globin Gene Affect Mrna Metabolism, Susan J. Baserga
Yale Medicine Thesis Digital Library
A common mutation causing thalassemia in Mediterranean populations is an amber (UAG) nonsense mutation at the 39th codon of the human β globin gene, the β-39 mutation. Studies of mRNA metabolism in reticulocytes from patients with β-39 thalassemia and studies using heterologous transfection systems have suggested the possibility that this mutation affects not only protein synthesis but also alters mRNA metabolism. This phenomenon has been investigated further by two approaches. A careful series of RNA expression studies were performed comparing expression of β-39 to β-normal (β-nl). These experiments led to the conclusion that the defect in expression of the β-39 …
Alteration Of The Adenylate Cyclase System In Specific Regions Of Brain Infected With Creutzfeldt-Jakob Disease, Susan Valley
Alteration Of The Adenylate Cyclase System In Specific Regions Of Brain Infected With Creutzfeldt-Jakob Disease, Susan Valley
Yale Medicine Thesis Digital Library
Creutzfeldt-Jakob (CJD) disease is an infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs and hamsters infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide mediated activation of adenylate cyclase which appears to be due to a greater coupling of stimulatory subunits (Ns) and not to a decreased coupling of inhibitory subunits (Ni) or to a change in the catalytic subunit of adenylate cyclase. In addition to the increase in Ns coupling, CJD infected membranes appear to be more fluidized than normal membranes especially in the cerebral cortex. Experiments with selected neurotransmitters …