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Analysis Of The Carbon-13 And Proton Nmr Spectra Of Bovine Chromaffin Granules, Edward P. Richards, Robert R. Sharp

Journal Articles

Natural abundance carbon-13 and proton NMR spectra of bovine chromaffin granules have been obtained and analyzed using computer simulation techniques. High resolution spectra show the presence of a fluid aqueous phase containing epinephrine, ATP and a random coil protein. The protein spectrum conrains unusually intense resonances due to glutamic acid and proline and has been simulated satisfactorily using the known amino acid composition of chromogranin A.

The lipid phase of chromaffin granules gives rise to intense, but very broad, resonances in the carbon-13 spectrum. Protons in the lipid phase are also observable as a very rapid component of the proton-free …

Molecular Mobilities Of Soluble Components In The Aqueous Phase Of Chromaffin Granules, Edward P. Richards, Robert R. Sharp

Journal Articles

NMR relaxation times have been used to characterize molecular motion and intermolecular complexes in the aqueous phase of bovine chromaffin granules. Partially relaxed '3C and proton spectra have been obtained at 3 and 25°C. T1 measurements of five protonated carbons on epinephrine (C:, Cs, C6, CHOH and NCH3) give a correlation time of 0.15 (10 -9) s at 25°C for the catechol ring and methine carbon, while the effective correlation time for the NCH3 group is somewhat shorter due to its internal degree of rotational freedom. Resonances of protonated carbons on the soluble protein chromogranin give very similar correlation times: …