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Full-Text Articles in Medicine and Health Sciences

Regulation Of Iga Class Switch Recombination In The I.29Μ B Cell Lymphoma By Cytokines And Inhibitors Of Poly(Adp-Ribose) Polymerase: A Thesis, Penny E. Shockett Sep 1993

Regulation Of Iga Class Switch Recombination In The I.29Μ B Cell Lymphoma By Cytokines And Inhibitors Of Poly(Adp-Ribose) Polymerase: A Thesis, Penny E. Shockett

GSBS Dissertations and Theses

Heavy chain isotype switch recombination is preceded by the appearance of RNA initiating 5' of the specific switch region which will undergo recombination. In an effort to understand the potential function of germline transcripts in switch recombination and the degree to which the regulation of germline transcripts correlates with the regulation of switching, we studied this process in the murine B-lymphoma cell line I.29μ, which in the presence of bacterial lipopolysaccharide (LPS) switches primarily to IgA and less frequently to IgE. Levels of α-germline transcripts initiating upstream of α switch (Sα) sequences are elevated in clones of this ...


Analysis Of And Role For Effector And Target Cell Structures In The Regulation Of Virus Infections By Natural Killer Cells: A Dissertation, Randy R. Brutkiewicz Sep 1993

Analysis Of And Role For Effector And Target Cell Structures In The Regulation Of Virus Infections By Natural Killer Cells: A Dissertation, Randy R. Brutkiewicz

GSBS Dissertations and Theses

The overall emphasis in this thesis is the study of the regulation of virus infections by natural killer (NK) cells. In initial analyses, vaccinia virus (VV)-infected cells were found to be more sensitive to NK cell-mediated lysis during a discrete period of time post-infection. This enhanced susceptibility to lysis correlated with enhanced triggering (but not binding) of the effector cells and a concomitant decrease in target cell H-2 class I antigen expression. Furthermore, VV-infected cells became resistant to lysis by allospecific cytotoxic T lymphocytes (CTL) at a time when they were very sensitive to killing by NK cells or ...


Sieb And Esc Genes Of Bacteriophage P22: A Dissertation, Koustubh Ranade Jun 1993

Sieb And Esc Genes Of Bacteriophage P22: A Dissertation, Koustubh Ranade

GSBS Dissertations and Theses

The superinfection exclusion gene (sieB) of Salmonella phage P22 was mapped using phage deletion mutants. The DNA sequence in the region was re-examined in order to find an open reading frame consistent with the deletion mapping. Several discrepancies from the previously published sequence were discovered. The revised sequence revealed a single open reading frame of 242 codons with six likely translation initiation codons. On the basis of deletion and amber mutant phenotypes the second of these six sites was inferred to be the translation initiation site of the sieB gene. The sieB gene encodes a polypeptide with 192 amino acid ...


Transport Of Nucleotide Derivatives Into Endoplasmic Reticulum And Golgiapparatus Derived Vesicles: A Dissertation, Caroline A. Clairmont May 1993

Transport Of Nucleotide Derivatives Into Endoplasmic Reticulum And Golgiapparatus Derived Vesicles: A Dissertation, Caroline A. Clairmont

GSBS Dissertations and Theses

In mammals, newly synthesized proteins destined for secretion are translocated cotranslationally into the lumen of the Endoplasmic Reticulum (ER). Once inside, these nascent polypeptide chains are bound by a lumenal ER protein called BiP (Immunoglobulin Binding Protein) or Grp 78 (Glucose Regulated Protein 78). It is hypothesized that this binding is necessary to protect the nascent chains until they are properly folded or assembled with other subunits. When the proteins are folded and assembled, they are released from BiP by a process that is dependent on ATP hydrolysis. Since ATP is synthesized mainly in the mitochondria, we hypothesized that there ...


Virus-Host Interactions In The Development Of Avian Leukosis Virus-Induced Osteopetrosis: A Dissertation, Rosalinda Gram Foster May 1993

Virus-Host Interactions In The Development Of Avian Leukosis Virus-Induced Osteopetrosis: A Dissertation, Rosalinda Gram Foster

GSBS Dissertations and Theses

Avian leukosis virus (ALV)-induced osteopetrosis is a proliferative disorder of the bone affecting the growth and differentiation of osteoblasts. Osteopetrosis is a polyclonal disease in which cells of the bone contain, on average, multiple viral DNA copies. Osteopetrotic bone is also characterized by the accumulation of unintegrated viral DNA, suggesting an atypical life cycle of the virus in the infected osteoblasts. To better understand virus-host interactions in the induction of osteopetrosis by ALVs, infected chick osteoblast cultures and osteopetrotic bone were examined for aspects of the virus life cycle and effects of infection on osteoblast function.

Levels of infection ...


Nuclear Structure Studied By Fluorescence Hybridization: Visualization Of Individual Gene Transcription And Rna Splicing: A Thesis, Yigong P. Xing Apr 1993

Nuclear Structure Studied By Fluorescence Hybridization: Visualization Of Individual Gene Transcription And Rna Splicing: A Thesis, Yigong P. Xing

GSBS Dissertations and Theses

The overall objective of this study has been to address some of the longstanding questions concerning functional organization of the interphase nucleus. This was achieved by using recently developed high-resolution fluorescence in situ hybridization techniques for a precise localization of specific DNA and RNA sequences in conjunction with immunocytochemistry and biochemical fractionation. This study is based on the philosophy that new insights may be gained by an approach that attempts to interrelate genomic organization, spatial arrangement of RNA metabolism, and nuclear substructure within the mammalian cell nucleus.

The nuclear distribution of an exogenous, viral RNA (Epstein-Barr Virus, EBV) within nuclear ...


Regulation Of Δ-Aminolevulinic Acid Synthase And Heme Oxygenase In Cultured Chick Embryo Liver Cells: Synergistic Induction Of Both Enzymes By Glutathimide And Iron And Repression Of Δ-Aminolevulinic Acid Synthase By Metalloporphyrins And Heme: A Dissertation, Edward Earl Cable Apr 1993

Regulation Of Δ-Aminolevulinic Acid Synthase And Heme Oxygenase In Cultured Chick Embryo Liver Cells: Synergistic Induction Of Both Enzymes By Glutathimide And Iron And Repression Of Δ-Aminolevulinic Acid Synthase By Metalloporphyrins And Heme: A Dissertation, Edward Earl Cable

GSBS Dissertations and Theses

Primary chick embryo liver cells were used to explore the regulation of δ-aminolevulinic acid synthase and heme oxygenase, the enzymes that catalyze the rate-limiting reactions of heme anabolism and catabolism, respectively. The general focus of the work was the exploration of the novel observation in which glutethimide and iron synergistically induced both δ-aminolevulinic acid synthase and heme oxygenase, a phenomenon that would not be predicted a priori. The course of events appeared to be: first, that heme synthesis was increased after addition of the glutethimide and that iron potentiated heme synthesis; second, the heme induced heme oxygenase five to ten ...


Dynamics Of Neuron-Specific Gene Expression During Development And In Response To Selective Lesions Of The Rat Central Nervous System: A Dissertation, Richard H. Melloni Apr 1993

Dynamics Of Neuron-Specific Gene Expression During Development And In Response To Selective Lesions Of The Rat Central Nervous System: A Dissertation, Richard H. Melloni

GSBS Dissertations and Theses

Synapse development and injury-induced reorganization in the nervous system have been extensively characterized morphologically, although, relatively little is known regarding the molecular and biochemical events that underlie these processes. In an attempt to better understand, at the molecular level, the role of the expression of synaptic proteins during synapse establishment and regeneration, this dissertation examines the dynamics of expression of the neuron-specific gene synapsin I during development and in response to selective lesions of the rat central nervous system. Synapsin I is the best characterized member of a family of nerve-terminal specific phosphoproteins implicated in the regulation of neurotransmitter release ...


Genetic Analysis Of The Saccharomyces Cerevisiae Centromere-Binding Protein Cp1: A Thesis, Daniel C. Masison Mar 1993

Genetic Analysis Of The Saccharomyces Cerevisiae Centromere-Binding Protein Cp1: A Thesis, Daniel C. Masison

GSBS Dissertations and Theses

CP1 is a sequence specific DNA-binding protein of the yeast Saccharomyces cerevisiae which recognizes the highly conserved centromere DNA element I (CDEI) of yeast centromeres. The gene encoding CP1, which was designated CEP1 for centromere protein 1, was cloned and sequenced. CEP1 encodes a highly acidic protein of molecular weight 39,400. CEP1 was mapped to a position 4.6 centiMorgans centromere distal to SUP4 on the right arm of chromosome X. Phenotypic analysis of cep1 mutants demonstrated that yeast strains lacking CP1 are viable but have a 35% increase in cell doubling time, a ninefold increase in the rate ...


Hiv-1 Sequences In The Establishment Of Chronic Virus Producers: A Thesis, Farah Mustafa Jan 1993

Hiv-1 Sequences In The Establishment Of Chronic Virus Producers: A Thesis, Farah Mustafa

GSBS Dissertations and Theses

Human immunodeficiency virus type 1 (HIV-1) infections have different patterns of expression in different T-cell lines. HIV-1 encodes regulatory as well as structural genes. The role of HIV-1 regulatory gene expression in determining different patterns of infection was explored in four T-cell lines: C8166, H9, A3.01, and Jurkat. The hypothesis being tested was that differences in the expression of regulatory genes would determine differences in the kinetics of infection.

To study patterns of regulatory and structural gene expression, RNA was isolated from cultures infected with HIV-1-NL4-3 (NL4-3). During the early and acute phases of infection, the absolute amounts of ...