Medicine and Health Sciences Commons^™

Numerical Investigation Of Subglottal Stenosis Effects On Human Voice Production, Dariush Bodaghi

Electronic Theses and Dissertations

This dissertation aimed to advance knowledge of how subglottal stenosis impacts voice production physiology. An in-house fluid-structure-acoustic interaction approach based on the hydrodynamic/acoustic splitting technique was employed. This technique was rigorously verified for simulating phonation by matching the acoustic behavior to a compressible flow solver for phonation-relevant geometries. Simulations of an idealized 2D vocal tract model demonstrated the effects of supraglottal acoustic resonance on vocal fold kinematics and glottal flow waveform. Results showed that the acoustic coupling between higher harmonics and formats generated pressure oscillations, modifying vocal fold dynamics and glottal flow rate.

A major novelty was the incorporation and …

On The Anti-Adipogenic Function Of Collagen Triple Helix Repeat-Containing Protein 1, Matthew E. Siviski

Electronic Theses and Dissertations

Adipogenesis is regulated by the coordinated activity of adipogenic transcription factors, including PPAR-gamma (PPARG) and C/EBP alpha (CEBPA). Thus, dysregulated adipogenesis predisposes adipose tissues to adipocyte hypertrophy and hyperplasia. We have previously reported that mice possessing a homozygous null gene mutation in collagen triple helix repeat-containing protein 1 (CTHRC1) have increased adiposity compared to wildtype mice, supporting the concept that CTHRC1 regulates body composition. Herein, we investigated the anti-adipogenic activity of CTHRC1. Using 3T3-L1 preadipocytes, we showed significantly reduced adipogenic differentiation in the presence of CTHRC1 commensurate to marked suppression of Cebpa and Pparg gene expression. In addition, CTHRC1 increased …

Minoritized Medical Students' Integration Of Professional Identities: A Phenomenological Study, Travis Erickson

Electronic Theses and Dissertations

This qualitative study fills a gap in the extant literature by exploring how medical students with minoritized identities make meaning about sense of self-definition and emerging professional identities while enrolled in medical school. More specifically, the focus of this study explored how minoritized medical students perceived their sense of self and dimensions of identity were shaped during their medical education, and what perceptions these medical students had about how they manage their professional identity development. Emergent themes included: (a) making connections between self-definition and professional identities, (b) past experience of difference shaping identities, (c) self-definition with complexity, (d) fluidity as …

Muscle Defects Lead To Skeletal Deformities In A Zebrafish Model Of Distal Arthrogryposis, Emily A. Tomak

Electronic Theses and Dissertations

Distal Arthrogryposis Type 1 (DA1) involves mild muscle weakness and limb skeletal abnormalities thought to be caused by paralysis in utero. Why the limbs are particularly affected in DA1 and the degree of paralysis that leads to these skeletal deformities in utero remains unclear. Several muscle genes are known to cause DA1, including MYLPF (myosin light chain phosphorylatable), which encodes a myosin light chain protein that binds close to the force-generating head of myosin heavy chains. The zebrafish mylpfa^-/- mutant displays a phenotype consistent with DA1, including impaired myosin activity, reduced muscle force overall, and complete fin paralysis. I …

A Quantitative Visualization Tool For The Assessment Of Mammographic Risky Dense Tissue Types, Margaret R. Mccarthy

Electronic Theses and Dissertations

Breast cancer is the second most occurring cancer type and is ranked fifth in terms of mortality. X-ray mammography is the most common methodology of breast imaging and can show radiographic signs of cancer, such as masses and calcifcations. From these mammograms, radiologists can also assess breast density, which is a known cancer risk factor. However, since not all dense tissue is cancer-prone, we hypothesize that dense tissue can be segregated into healthy vs. risky subtypes. We propose that risky dense tissue is associated with tissue microenvironment disorganization, which can be quantified via a computational characterization of the whole breast …

Scaling Up The Relevance Of Land-Sea Connections In Coastal Bacteria Pollution Vulnerability, Bea E. Van Dam

Electronic Theses and Dissertations

Bacteria pollution closures of Maine’s coastal shellfish harvest areas have substantial negative consequences for coastal businesses and communities. Sustainability solutions for Maine’s shellfish harvesting areas and businesses require new types of knowledge and information to protect water quality and public health while avoiding unnecessary fishery closures. Coastal management agencies have interests in tools to support science-based management decision-making related to pollution and sustainability solutions for businesses and communities.

Prior research into land-sea connections has demonstrated uses of geographic information and statistical methods to facilitate management and science communication. Research in Maine has focused on identification and comparison of attributes influencing …

The Relationship Between Intuitive Eating And Body Mass Index And Diet Quality In College Students, Ashley A. Reynolds

Electronic Theses and Dissertations

Objective

The objective of this research was to explore the influence of intuitive eating (IE) on body mass index (BMI) and diet quality (DQ) among college students.

Methods

A cross-sectional convenience sample of college students completed an online survey consisting of the short-Healthy Eating Index (SHEI) and the Intuitive Eating Scale-2 (23 items). Body mass index was calculated using self-reported height and weight. Descriptive statistics were used to assess group characteristics. A linear regression was used to test if IE scores significantly predicted BMI. An independent t-test was used to compare BMIs of students with IE scores above and below …

Nanocellulose Based Foams For Low-Cost Disposable Medical Applications, Dominic Kugell

Electronic Theses and Dissertations

Polyurethane foams have been a staple material for their use in medical positioners, such as post-surgery elevation pillows as well as specific tailored positioners for their use during surgery. Polyurethane foams are preferred because of their lower cost compared to other petroleum derived foams, their versatility, and suitable mechanical properties. However, the environmental impact, including both cost and perception, of these foams is immense. Therefore, alternatives are being explored with biopolymers emerging as a promising class of materials. Cellulose is one such polymer that has recently demonstrated desirable properties. In this study, cellulose nanofibrils (CNF), a household foaming agent, and …

The Relationship Between Social Cognition And Social Behavior Of Young Adults With Autism Spectrum Disorder Using The Ucla Peers® For Young Adults Social Skill Program, Meaghan Balsdon

Electronic Theses and Dissertations

Research indicates that social communication impairments are a defining and persistent feature of Autism Spectrum Disorder (ASD; Centers for Disease Control and Prevention, 2022). Social communication consists of what an individual knows about social skills (i.e., social cognition) and how they observably respond in social situations (i.e., social behavior). It is difficult to gain a comprehensive assessment of social communication because social cognition assessments are prone to inaccuracies due to poor metacognitive skills in individuals with ASD (DeBrabander et al., 2021; Cederlund et al., 2010; Vickerstaff et al., 2006). Inaccurate reporting of social cognition leads to potential discrepancies in observable …

The Effects Of Peers For Young Adults On Anxiety And Quality Of Life For Young Adults With Autism Spectrum Disorder, Alysha Cecile Dagg

Electronic Theses and Dissertations

Social skills deficits as well as comorbid anxiety are two characteristics commonly experienced by people with Autism Spectrum Disorder - Level 1 (ASD-1; American Psychiatric Association, 2013). These characteristics are also both contributors to a lower quality of life for young adults (Smith et al., 2019). The current study aimed to identify how the quality of life and anxiety are affected by social skills intervention, specifically the PEERS® for Young Adults program (Laugeson, 2017). PEERS® for Young Adults is an evidence-based social skills intervention intended to support individuals with ASD-1 (Laugeson, 2017). Prior research has demonstrated its success in both …

A Social And Ecological Approach To Mosquito Species Distribution Across Land Use In Bangor, Maine, Megan L. Schierer

Electronic Theses and Dissertations

Mosquitoes are ubiquitous pests and infectious disease vectors. However, not all mosquito species bite humans, or are competent pathogen vectors between bloodmeal hosts. Along with climatic variables like temperature and rainfall, mosquito species distribution is determined by aquatic habitat availability for juvenile mosquito development, and terrestrial habitat and host availability for adult mosquitoes. There is variation in the preferred aquatic habitat for gravid female oviposition and subsequent larval development. Some mosquito species’ oviposition and development are associated with ephemeral water sources (e.g., floodplains), others prefer more permanent water sources (e.g., bogs or vernal pools). Other mosquitoes have evolved to occupy …

Hybrid Power Spectral And Wavelet Image Roughness Analysis, Basel White

Electronic Theses and Dissertations

The Two-Dimensional Wavelet Transform Modulus Maxima (2D WTMM) sliding window methodology has proven to be a robust approach, in particular for the extraction of the Hurst (H) roughness exponent from grayscale mammograms. The power spectrum is a computational analysis based on the Fourier transform that can be used to estimate the roughness of a scale-invariant image or region via the calculation of H. We aim to examine how the calculation of H in fractional Brownian motion (fBm) images and mammograms can be improved. fBm images are generated for H ∈ [0.00,1.00] for testing through the previous 2D …

The Art Of Surviving: Alchemy Of Healing Trauma In Relation To Identity: A Self Study., Rebecca Morgan

Electronic Theses and Dissertations

The following thesis explores trauma’s physical and psychological aspects concerning identity as an artistic practice. Through exploring materials, subject matter, and media, my approach to trauma is based on personal and socially engaged experiences and my attempt to re-conceptualize that experience through the language of contemporary art. Extensively this work is governed by childhood memories and the critical aspect of being raised as a female in a patriarchal society. Being raised female comes with a certain number of expectations and requirements. This work creates a physical and spiritual connection between trauma and the identity of what is female. Discussing these …

Primary Cilia Of The Cardiac Neural Crest & Hedgehog-Mediated Mechanisms Of Congenital Heart Disease, Lindsey A. Fitzsimons

Electronic Theses and Dissertations

Elimination of primary cilia in cardiac neural crest cell (CNCC) progenitors is hypothesized to cause a variety of congenital heart defects (CHDs), including atrioventricular septal defects, and malformations of the developing cardiac outflow tract. We present an in vivo model of CHD resulting from the conditional elimination of primary cilia from CNCC using multiple, Wnt1:Cre-loxP, neural crest-specific systems, targeting two distinctive, but critical, primary cilia structural genes: Intraflagellar transport protein 88 (Ift88) or kinesin family member 3A (Kif3a). CNCC loss of primary cilia leads to widespread CHD, where homozygous mutant embryos (MUT) display a variety of outflow tract malformations, septation …

Assessment Of Cellular Alignment And Proliferation On Cellulose Nanofibril(Cnf) Films, Sahar Roozbahani

Electronic Theses and Dissertations

Research on novel biomaterials to address current limitations associated with bone grafting is currently of high demand. Current treatment options for bone regeneration are facing challenges such as donor site morbidity and limited availability, immune rejection, and disease transmission. Research conducted on natural polymers to mimic Extracellular matrix (ECM)structure of bone tissue demonstrated their strong potential to spur bone regeneration. Engineered scaffolds that are inspired by oriented collagen/Hydroxyapatite (HA)structure in natural bone are promising biomaterials that can provide an ECM like structure for primitive mesenchymal cells to grow and differentiate to the bone cells. Cellulose nanofibril (CNF) is a natural …

Pacing Variability And Performance In A 100 Mile Ultra Marathon, Brendan Gilpatrick

Electronic Theses and Dissertations

The purpose of this research was to explore the relationship between pacing variability and performance during a 100 mile trail race with significant changes in elevation. Changes in pace throughout an endurance event of this length could lead to insight into the relationship of early pacing on overall performance and placing among finishers. Due to variables like changes in terrain or weather it could prove difficult to construct a way to analyze data from these races. Race data from a loop style course with significant elevation change was used to determine if 1). There were significant changes in pace per …

The Use Of Medical Cannabis In Clinical Practice, Arianne Kryskow

Electronic Theses and Dissertations

Medical Cannabis is receiving renewed interest in clinical practice due to the gradual increase over the last few decades of cannabis legalization and high-quality research on the potential benefits of cannabis for treating a variety of conditions (NASEM, 2017; Nursing Care of the Patient, 2018). However, the pace of medical cannabis legalization and research are outpacing the training for medical providers, leaving gaps in their confidence and ability to safely guide patients using medical cannabis (NCSBN, 2018). Medical providers are increasingly fielding questions from patients regarding the use of medical cannabis for conditions commonly seen in clinical practice, but many …

Deciphering The Perpetual Fight Between Virus And Host: Utilizing Bioinformatics To Elucidate The Host's Genetic Mechanisms That Influence Jc Polyomavirus Infection, Michael P. Wilczek

Electronic Theses and Dissertations

JC polyomavirus (JCPyV) is a human-specific pathogen that infects 50-80% of the population, and can cause a deadly, demyelinating disease, known as progressive multifocal leukoencephalopathy (PML). In most of the population, JCPyV persistently infects the kidneys but during immunosuppression, it can reactivate and spread to the central nervous system (CNS), causing PML. In the CNS, JCPyV targets two cell types, astrocytes, and oligodendrocytes. Due to the hallmark pathology of oligodendrocyte lysis observed in disease, oligodendrocytes were thought to be the main cell type involved during JCPyV infection. However, recent evidence suggests that astrocytes are targeted by the virus and act …

Empathic Instruction Through Literary Narratives: A Quasi-Experimental Study Of An Occupational Therapy Course, Cavenaugh P. Kelly

Electronic Theses and Dissertations

This study examines concerns within the field of occupational therapy on the growing disconnect between the profession’s roots and espoused beliefs in empathic-centered care, and the modern realities of health care. In particular, the study examined whether the empathy levels of occupational therapy students would change after a course involving the close reading of literary narratives. Close reading of literary narratives has correlated with improved levels of empathy. Empathy is defined as a four-step dynamic process involving Theory of Mind (ToM), emotional resonance, emotional regulation, and empathy as a willful act. Initial study of the proposed curriculum found improved scores …

The Correlation Of Biometrics And Game Performance In Division I Collegiate Women's Soccer Team, Finn Ducker

Electronic Theses and Dissertations

In competitive sports, the ultimate goal is to win as many matches, competitions, or games as possible. In an attempt to optimize this goal, the use of data analytics has risen. Soccer, a particularly popular sport across the globe, has been using data analytics for this optimization for multiple decades. One common practice for this data collection is the use of heart rate monitors paired with GPS tracking in order to collect data. Some of the data collected includes training load, total distance, sprints, and time spent in various heart rate percentage zones. Additionally, InStat Index has been used to …

Handling And Manipulation Of Water- And Air- Borne Biological Samples Using Liquid-Infused Surfaces, Daniel P. Regan

Electronic Theses and Dissertations

Research on novel materials to handling water- and airborne samples for biological threats analysis is in great demand due to the COVID-19 pandemic. Work conducted on a new field of material science, called liquid-infused surfaces, demonstrate strong potential for the handling and manipulation of biological samples. As a result of the field’s infancy, only a limited number of studies have explored how liquid-infused surfaces can apply droplet manipulation strategies to address real-world problems. Presented in this dissertation are two platforms that leverage liquid-infused surfaces to address the challenges associated with handling water- and airborne biological samples. When dealing with waterborne …

Toward Improving Understanding Of The Structure And Biophysics Of Glycosaminoglycans, Elizabeth K. Whitmore

Electronic Theses and Dissertations

Glycosaminoglycans (GAGs) are the linear carbohydrate components of proteoglycans (PGs) that mediate PG bioactivities, including signal transduction, tissue morphogenesis, and matrix assembly. To understand GAG function, it is important to understand GAG structure and biophysics at atomic resolution. This is a challenge for existing experimental and computational methods because GAGs are heterogeneous, conformationally complex, and polydisperse, containing up to 200 monosaccharides. Molecular dynamics (MD) simulations come close to overcoming this challenge but are only feasible for short GAG polymers. To address this problem, we developed an algorithm that applies conformations from unbiased all-atom explicit-solvent MD simulations of short GAG polymers …

Beyond The Brain: A Study Of Α-Synuclein's Role In Bone And Adipose Tissue, Carolina A. Figueroa

Electronic Theses and Dissertations

α-Synuclein is a polypeptide encoded by the Snca gene, highly expressed in neurons, but it is also found in bones and adipose tissue. Co-expression analysis showed that Snca regulates skeletal homeostasis, and its deletion reduced estrogen deficiency-induced bone loss and weight gain. It is a major component of Lewy bodies (LB) in Parkinson’s disease (PD), leading to progressive immobilization and a range of nonmotor symptoms, including osteopenia, body composition alterations and insulin resistance. This thesis aimed to determine α-Synuclein’s intrinsic role in bone and adipose homeostasis. We discussed the PD pathophysiology emphasizing aspects of bone health and metabolism. By using …

The Impact Of Cooking Knowledge, Attitude, Behavior, And Food Security Status On Diet Quality Of College Students At The University Of Maine, Angela I. Czup

Electronic Theses and Dissertations

Food insecurity in the United States is a complex issue. Nutrition interventions and studies are often designed for high-risk populations with others being overlooked. Until recently, few studies and interventions have focused on college-aged students. In order to understand the nutrition-related needs of students at the University of Maine, it is necessary to determine their cooking knowledge, attitudes, behaviors, and food security status. Study participants included college sophomores, juniors, and seniors [n=16 sophomores, (38.1%), n=15 juniors, (35.7%), n=11 seniors, (26.2%)]. The study design was cross-sectional where participants completed a combined Qualtrics online survey and the Automated Self-Administered 24-Hour Dietary Assessment …

Health Insurance Plan Design And Chronic Disease Management, Daniel E. Feldman

Electronic Theses and Dissertations

Each year, Americans spend more money on health care than any other industrialized nation, despite comparable mortality rates for people with risk factors for heart disease. The reasons for this lack of health care value in the US are numerous and complex – including market distortions like supplier-inflated pricing and regulatory structures that enable consumers to utilize ubiquitous, high-cost medical technologies that yield uncertain benefits. Health insurance, once thought to be an insignificant contributor to rising health spending, has changed considerably in the past few decades in ways that make it more accessible and more generous in coverage. Health insurance …

A Comprehensive Model Of Stress-Induced Binge Eating: The Role Of Cognitive Restraint, Negative Affect, And Impulsivity In Binge Eating As A Response To Stress, Rachael M. Huff

Electronic Theses and Dissertations

As obesity-related health issues account for the leading number of deaths in America, and these illnesses are even more prevalent in those that binge eat, treating obese individuals with Binge Eating Disorder (BED) has become a matter of life and death. Stress, negative affect, cognitive restraint, and impulsivity have all been implicated in binge eating symptomatology, suggesting that these may be key variables to consider when developing a comprehensive model (Groesz et. al., 2012; Friese et. al., 2015; Galanti et. al., 2007; Gay et al., 2008; Racine et. al., 2015; Leehr et. al., 2015). This project aims to further examine …

Zebrafish As A Model To Understand The Impact Of Inactivity And Neuromuscular Electrical Stimulation On Duchenne Muscular Dystrophy, Elisabeth Kilroy

Electronic Theses and Dissertations

Skeletal muscle plasticity is imperative for functional adaptation to changing demands in activity. Although a great deal is known about the structural and functional plasticity of healthy skeletal muscle, far less is known about plasticity in diseased muscle. Here, we combined the power of the zebrafish model with the adaptability of neuromuscular electrical stimulation (NMES) to study the basic mechanisms of plasticity in the zebrafish model of Duchenne Muscular Dystrophy (DMD). Four NMES paradigms, defined by their frequency, delay, and voltage, were designed to emulate the repetition and load schemes of human resistance training programs. Additionally, two inactivity paradigms were …

Does Circularizing Source-Separated Food Waste Present A Risk To Our Food?, Astha Thakali

Electronic Theses and Dissertations

About a third of the food produced annually is wasted. Food waste recycling can be a way to close the loop and attain a more sustainable food system, however, the system must be carefully monitored and managed to avoid the introduction and build-up of contaminants. To study the potential presence of contaminants in food waste, source-separated food waste was collected and screened for five classes of contaminants (physical contaminants, heavy metals, halogenated organic contaminants, pathogens, and antibiotic resistance genes) from two separate regulatory environments (voluntary vs mandated food separation). The regulatory environment did not affect the level of contamination, except …

Discovery Of Distinct Mechanisms Underlying The Relationship Between Drug Taking And Predisposing Behaviors, Tyler A. Roy

Electronic Theses and Dissertations

Drug addiction is a heritable disease characterized by compulsive drug use. The biological mechanisms driving addiction remain largely unknown.¹ Previous studies show shared genetic mechanisms underlying addiction risk phenotypes such as anxiety, depression, and novelty/sensation seeking.^2,3 Therefore, high-throughput behavioral screening of these traits in single gene knockout mice can allow for the rapid detection of addiction risk candidate genes and mechanisms. Many of these traits are represented in the Knock-Out Mouse Program (KOMP) phenotyping pipeline. Of the initial two hundred twenty-one strains screened in this program, we tested nineteen phenodeviant knock-out mouse strains with C57BL/6NJ controls (N = …

The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga

Electronic Theses and Dissertations

Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …