Medicine and Health Sciences Commons^™

The Effects Of Prenatal Cannabis Exposure On The Basolateral Amygdala, Karen Kw Wong

Undergraduate Student Research Internships Conference

Clinical and preclinical studies indicate prenatal cannabis exposure (PCE) pathologically affects fetal brain development and may increase vulnerability to neuropsychiatric disorders, including schizophrenia and mood/anxiety disorders. In review research from our lab suggests that fetal exposure to Δ9-THC sex-selectively impairs mesocorticolimbic (MCL) circuit function. However, there is a distinct lack of focus on PCE models on the BLA. The BLA plays a central role within the MCL where it directly interacts with the VTA, PFC and HIPP. Importantly, our model exhibits significant VTA hyperdopaminergic activity, and sex-specific alterations to PFC/HIPP glutamate firing, alongside region- and sex-specific changes in dopamine (DA), …

Open-Source And 3-D Printed Autoinjector, Anjutha Selvaraj

Undergraduate Student Research Internships Conference

Autoinjectors have become popular modern injectable medical devices used as drug delivery systems. Due to its ease, capability and reliability compared to other conventional injectable medical devices, the market and manufacturing demand for autoinjector devices are increasing at a staggering rate. Although autoinjectors can offset healthcare treatment costs through self-administered medication, they are expensive for consumers. This paper describes the design and manufacture of a spring-driven and 3-D printed autoinjector using open-source hardware. By making it an open-source resource for everyone, the barrier of advanced research in design and production can be lifted, thus making it easily manufacturable and cost-effective. …

Rheological Characterization Of Blood-Mimicking Fluids For Use In Particle Image Velocimetry, Anorin S. Ali

Undergraduate Student Research Internships Conference

Blood-mimicking fluids (BMFs) are often used to investigate blood flow using physical replicas of vessels with cardiovascular disease. Particle image velocimetry (PIV) is used with silicone poly-dimethyl siloxane (PDMS) vascular models to visualize this flow. The challenge is creating a blood-mimicking fluid that matches the viscosity profile, viscoelasticity, and density of whole blood while also matching the refractive index (RI) of PDMS. Water-glycerol solutions are commonly used with sodium iodide (NaI) added to increase the RI without changing viscosity. However, NaI is expensive, stains easily, and turns fluids from optically clear to yellow in less than a day. Furthermore, without …

Ex-Smokers With And Without Copd: Investigating Ct Pulmonary Vascular, Airway, Pulmonary Artery And Aorta Measurements, Vedanth Desaigoudar, Paulina V. Wyszkiewicz, Alexander M. Matheson, Maksym Sharma, Marrissa J. Mcintosh, Harkiran K. Kooner, David G. Mccormack, Grace Parraga

Undergraduate Student Research Internships Conference

RATIONALE: Pulmonary hypertension is characterized by increased pressure in the pulmonary artery, and is a key contributor to worsening symptoms in individuals with chronic obstructive pulmonary disease (COPD). The pulmonary artery to aorta diameter ratio (PA:Ao), measured using computed tomography (CT), is a biomarker of pulmonary hypertension; however, longitudinal changes in this measurement and its relationship to pulmonary vascular and airway structural changes is not well understood. Our objective was to investigate longitudinal changes in PA:Ao and its relationship with CT pulmonary vascular and airway abnormalities, airflow limitation and exercise-capacity.

Participant Outcomes From The Virtual More Than Words® Program, Jodi Friesen

Undergraduate Student Research Internships Conference

More Than Words® is a program run through the Hanen Center, for caregivers of children with Autism or social communication disorders. Through secondary data analysis of interview transcripts and a survey, this research attempted to outline the reported child and caregiver outcomes from the virtual More Than Words® program.

Optimizing Rna Isolation And Histology Protocols For Characterization Of Preclinical Models Of Tendon Disease, Deirdre Finnigan, Melika Meshkat, Ali Naeij, Dawn Bryce, Matthew W. Grol

Undergraduate Student Research Internships Conference

Tendons join muscle to bone and are essential for posture and movement. Tenocytes are the resident tendon cells. Isolating RNA from small animal models such as mice can be difficult, especially from tendon, as much of the time, they are extremely small samples. Downstream analysis such as qPCR and especially RNA sequencing require greater quantity and quality of RNA than is often achievable. RNA degradation is extremely rapid following tendon isolation, with RNA quality decreasing as time increases between the collection of tendons and RNA isolation. Even tendons whose RNA was isolated immediately following tendon isolation had imperfect quality. The …

Microvascular Regulation Between Two Hemodynamic Steady States In The Forearm And Forehead Using Wavelet Phase Coherence, Yuki Bao

Undergraduate Student Research Internships Conference

Microvascular mechanisms that regulate the microcirculation can be observed within blood flow as coupled oscillations operating over a wide range of different frequencies and time scales. Abnormally discordant interactions between regulation mechanisms can cause derangement of microvascular flow patterns, which has been suggested to be indicative of increased cardiovascular disease risk. Based on the premise that interactions between microvascular mechanisms are key to understanding flow regulation and disease progression, wavelet-based phase coherence was used to characterize the relationship between cardiac, respiratory, myogenic, neurogenic, and metabolic regulation across different microvascular beds and physiological conditions.

Steady state cutaneous microvascular blood flow data …

Resistance Training Improves White Matter Structural Connectivity In Older Adults At-Risk For Cognitive Decline, Ryu Lien

Undergraduate Student Research Internships Conference

Diabetes is a global health concern that impacts 415 million people worldwide. Individuals who are at-risk for diabetes (characterized by high blood glucose and/or being overweight) have white matter atrophy, decreased cognitive function, and an increased risk of Alzheimer’s disease (AD). Recently, resistance training (RT) has been shown to lower white matter atrophy and white matter lesion volume. However, investigating changes in white matter tracts is complex, hence previous findings remain inconclusive. Diffusion tensor imaging (DTI) serves as a highly sensitive tool that enables visualization and characterization of white matter tracts and has the potential to combat this complexity. The …

Rapid Changes In Vascular Compliance Contribute To Cerebrovascular ​Adjustments During Vasovagal Syncope (Vvs), Aleena Sajid

Undergraduate Student Research Internships Conference

Background: The rapid reduction in arterial pressure (AP) during VVS is associated with a decline in diastolic cerebral blood velocity (CBV) and maintained systolic CBV. We tested the hypothesis that this response may represent increased cerebrovascular compliance (Ci) in the presence of rapid hypotension.

Methods: AP and CBV were recorded from 14 otherwise healthy patients (34 ± 11 years) at rest and during head-up tilt (80°, 5-28 min). Baseline, mid-tilt, and VVS were analyzed. Individual AP and CBV waveforms were input into a modified Windkessel model to calculate Ci.

Results: Upright posture resulted in a decrease in CBV (-9 ± …

Exploring The Structural And Functional Effects Of Pediatric Hydrocephalus On The Hippocampus, Lili Meng

Undergraduate Student Research Internships Conference

Hydrocephalus is one of the most common pediatric neurological problems that requires brain surgery in children. Pediatric hydrocephalus is characterized as an abnormal cerebrospinal fluid (CSF) buildup in the brain’s ventricles due to ineffective CSF reabsorption. When this fluid builds up in these cavities, it increases intracranial pressure and has a direct mass effect on the surrounding brain tissue and structures. Ultimately, various functions are affected including—but not limited to—vision, learning, memory, motor control, and hearing. Despite current literature suggesting that hydrocephalus may be associated with reduced hippocampal volume among rats and human adults, findings remain limited in the human …

Rna Isolation In Duchenne Muscular Dystrophy (Dmd) Mice Models, Salem Abu Al-Burak

Undergraduate Student Research Internships Conference

Fibrosis is a progressive and typically irreversible disease process characterized by the excessive deposition of collagen in organs and in tissues of the musculoskeletal (MSK) system^1,2. This process, which causes loss of organ and tissue function, can be initiated by micro-traumas³, an excessive and/or prolonged immune response¹, the activation and proliferation of fibrosis-inducing progenitor cells⁴, and a pro-fibrotic extra-cellular microenvironment⁵. In parallel with the events that initiate fibrosis, genetic or environmental influences may cause cells and tissues to become predisposed to fibrosis development prior to initiation. This suggests that these …

Parkinson’S Disease & Difficult Conversations, Emily Brinovec

Undergraduate Student Research Internships Conference

No abstract provided.

Smart Indigenous Youth, Jamin Patel

Undergraduate Student Research Internships Conference

There is evidence that indicates the immense benefits of physical activity for the physical and mental health of youth. To respond to the youth mental health crisis in Indigenous communities in Canada, a culturally appropriate, land-based active living program called Smart Indigenous Youth (SIY) was developed by the Digital Epidemiology and Population Health Laboratory (DEPtH Lab). SIY is a longitudinal digital health community trial that applies the concept of citizen science to assess land-based active living’s impact on Indigenous youth mental health. My project examined the challenges to mental health faced by youth living in Indigenous Rural Communities during the …

A Content Analysis Of Anti-Doping Sciences And Interventions, Yuan Wang

Undergraduate Student Research Internships Conference

No abstract provided.

Effect Of A Nutrition And Exercise Lifestyle Intervention Program (Nelip) With A Family Based Behavioural Treatment (Fbbt) During Pregnancy And Postpartum On The Prevalence Of Large Infants Up To 1 Year Of Age, Madeline E. Hubbard

Undergraduate Student Research Internships Conference

Overweight (OW; body mass index [BMI] of 25.0 to 29.9 kg/m² ), and obesity (OB; BMI of 30 and greater kg/m²) are conditions where excessive fat accumulation presents a risk to health. Prepregnancy BMI and maternal excessive gestational weight (EGWG) gain are risk factors for higher infant birthweight and childhood OW or OB. The Nutrition and Exercise Lifestyle Intervention Program (NELIP) is a combined intervention designed to prevent EGWG in individuals with OW and OB. This study was a convenience sample from a larger trial using the NELIP with family based behavioural treatment (FBBT) to increase program adherence. The objective …

Menstrual And Fertility Tracking Apps And The Post Roe V. Wade Era, Samantha T. Campanella

Undergraduate Student Research Internships Conference

In the first section of the paper, I will place current conversations about data privacy within the broader context of restrictions that have been placed on reproductive rights by examining historical trajectories. Emphasis will be placed on the historical trajectory of how past policies and ideologies have worked against Roe v. Wade, and how this trajectory contributes to a decrease in access to abortions. In addition, recent news stories have documented the overturning of Roe v. Wade in several jurisdictions within the United States, which confirms the criminalization of abortion. In light of this, experts have raised awareness about the …

Functional Characterization Of A High-Throughput In Vitro Model To Predict Faecal Microbiota Transplantation (Fmt) Donor Success, Catherine M. Andary

Undergraduate Student Research Internships Conference

Faecal microbiota transplantation (FMT) involves the administration of donor faecal matter to a diseased recipient with the goal of remodeling the host microbiome to provide health benefits. In recent years, FMT has emerged as a potential therapy for a variety of microbiome-associated diseases such as atherosclerosis. Trimethylamine (TMA) is an atherosclerosis-linked metabolite generated by the gut microbiota from dietary precursors which is then oxidized to trimethylamine N-oxide (TMAO) by the liver, contributing to increased gut permeability. It has been shown that FMT may alter or restore the gut microbiome of recipients to reduce plasma TMAO levels. Despite its potential, the …

Assessing The Impact Of Lipopolysaccharide On Learning And Memory In Rats, Anahat Luthra

Undergraduate Student Research Internships Conference

The immune system and the central nervous system (CNS) have a bi-direction relationship, modulating one another.⁴ Proinflammatory cytokines released from CNS immune cells have an impact on cognitive processes such as learning and memory.¹ Liposaccharide (LPS), a cell wall component of Gram-negative bacteria, which is used to activate proinflammatory cytokine release has been found to impact learning and memory processes, such as in the anticipatory nausea paradigm (ANP).² Anticipatory nausea and vomiting is that which may occur before a chemotherapy treatment session begins in a patient who has had chemotherapy before. It is caused by triggers like …

Intracranial Eeg Patient Analysis: Seizure Reduction In Refractory Epilepsy Following Depth Electrode Insertion, Shreyashish Roy-Chowdhury

Undergraduate Student Research Internships Conference

Rationale: The implantation effect describes an immediate reduction in seizure frequency following the insertion of intracranial electrodes. There is limited information regarding which patients will experience a reduction of seizures and the reason for the implantation effect. This study focuses on describing predictor factors for the reduction in seizure frequency in patients with medically resistant epilepsy implanted with depth electrodes.

Methods: We conducted a retrospective chart review of 89 patients who underwent intracranial electroencephalography (iEEG) monitoring with depth electrodes. Sixty-five of these patients had no subsequent therapeutic interventions and had a 3-6 month follow-up. Patients were grouped as either responders …

Learning From Lived Experience: Substance Use Policies, Emergency Shelters, And Harm Reduction In London, On, Jesse Cram, Dena Salehipour

Undergraduate Student Research Internships Conference

Since 2018, the number of overdoses reported by London housing and emergency shelter agencies to the city of London has increased by 790%.These agencies currently have inconsistent overdose response policies that lack research-based support, and want to establish comprehensive best practices that support those involved at all levels.

Through a community based course, we initially collected and analyzed survey data from managers and staff from various London housing and emergency shelter agencies with the aim of understanding the limitations of the current approaches to the opioid crisis. Wanting to expand on this, our current project focuses on those who are …

Reporting Standards For Machine Learning Research In Type 2 Diabetes, Grace Kang

Undergraduate Student Research Internships Conference

In this project, three people scored 90 papers on machine learning predictive models for type 2 diabetes to assess their adherence to TRIPOD, MI-CLAIM, and DOME reporting guidelines.

Return To Physical Activity Post-Total Knee Arthroplasty And Total Hip Arthroplasty, Bob T. Gao, Ashley Lowndes, Denise M. Connelly

Undergraduate Student Research Internships Conference

No abstract provided.

Acoustic Reflex Activation And Its Effect On Middle Ear Function, Austen Schmoll

Undergraduate Student Research Internships Conference

Previous studies have suggested larger magnitudes and lower thresholds in ipsilateral acoustic reflexes when compared to contralateral acoustic reflexes. This pilot study explored these effects by measuring how sound is transmitted through the middle ear. Middle ear absorbance was measured in the ipsilateral and contralateral conditions with and without activation of the acoustic reflex in normal hearing adults. Data showed that ipsilateral acoustic reflex thresholds (ART) were approximately 5 dB lower than contralateral ARTs. The magnitude of the acoustic reflex was shown to be larger in the ipsilateral condition. Results suggest that there is an evident contrast between ipsilateral and …

In-Vitro Validation Of Intratumoral Modulation Therapy For Glioblastoma, Abdulla Elsaleh

Undergraduate Student Research Internships Conference

Intratumoral modulation therapy (IMT) is a novel electrotherapy used to treat brain cancer tumours using electric fields applied directly to the tumours through implanted electrodes. Previous research has validated IMT's effectiveness and provided computer-simulated optimizations for IMT electric fields. This work validates these computer optimizations in-vitro, using a PCB construct to deliver electric fields, and bioluminescence imaging to assess cell viability.

We found electric field strength to correlate with cell viability, and found that rotating (phase-shifted) electric fields did not produce significant improvements in IMT efficacy. Future work will investigate different IMT frequencies and other parameters, while providing biological replicates …

An Analysis Of Clinical Research Enrollment Decisions In Post-Ebola Guinea, Alisha Kabani

Undergraduate Student Research Internships Conference

In the aftermath of the West Africa Ebola outbreak, many grew familiar with the term “Ebola business”, a phrase serving as a shorthand for an assemblage of practices and possibilities that stood for the profitability potential of the deadly Ebola virus in the country. This is the backdrop for a CIHR-funded qualitative study aimed at advancing the understanding of whether and how those approached for clinical research post-Ebola, experienced those invitations and associated benefits.

My role consisted of supporting the analysis and expanding evidence-based understanding of when and on what basis, participants in foreign-funded and remunerated studies in high poverty …

Screen-Viewing Among Young Children In Childcare: An Updated Systematic Review, Leann Blake

Undergraduate Student Research Internships Conference

Screen-viewing is one of the main forms of sedentary activity in which young children engage (De Decker et al., 2013). Despite health risks of sedentary behaviour in the early years being well documented (Statistics Canada, 2019), there remains a lack of research focusing on sedentary activities within the context of childcare settings in which children participate. The purpose of the current systematic review is to update and expand on Vanderloo’s (2014) systematic review and provide a synthesis of all relevant research on the levels of screen-viewing among young children (0-5 years) attending childcare (i.e., centre-and home-based childcare). In addition, this …

Effect Of Capacity Building Interventions On Classroom Teacher And Early Childhood Educator Physical Activity And Fundamental Movement Skills Related Self-Efficacy, Knowledge, And Attitudes: A Systematic Review And Meta-Analysis, Ameena Haddara, Matthew Bourke, Aidan Loh, Kendall A. Saravanamuttoo, Brianne A. Bruijns, Patricia Tucker

Undergraduate Student Research Internships Conference

Generalist teachers and early childhood educators (ECEs) play an important role in promoting physical activity and fundamental movement skills in children. However, teachers face several barriers to promoting physical activity and fundamental movement skills including inadequate training and professional development. Therefore, this study aimed to determine the efficacy of capacity building interventions on teachers’ and ECEs’ physical activity and fundamental movement skills related knowledge, self-efficacy and attitudes.

The search revealed 22 studies which reported on 25 unique samples. The most common capacity building intervention component implemented was training/professional development. The results of this study revealed that capacity building interventions are …

An Environmental Scan Of Weight-Inclusive Resources, Sara A. Bozzo

Undergraduate Student Research Internships Conference

This summer, alongside another USRI student, I began to further explore weight-inclusive physical activity. This approach to movement emphasizes that health and well-being can be achieved by anyone, regardless of their size, if provided with accessible, non-stigmatizing environments and support. Though this approach has increased in prevalence over the years, little is known about the application of weight-inclusive approaches in real-world contexts. Using an environmental scan protocol, this study will aim to examine the breadth of online weight-inclusive resources and services within grey literature (internet, social media, experts); synthesize and evaluate the content within identified materials; and discover gaps in …

The Effects Of The Aromatase Inhibitor, Letrozole, On Lithium Chloride (Licl)-Induced Conditioned Disgust Behaviour (Anticipatory Nausea) In Male Rats, Vangel Matic

Undergraduate Student Research Internships Conference

Anticipatory Nausea (AN) is a form of classical conditioning in which the effects of a nausea-inducing toxin become associated with a specific environmental context. AN is often experienced by individuals receiving chemotherapy treatment, whereby the emetic effects of the chemotherapy agents become associated with the treatment context (i.e. hospital, clinic), such that exposure to the context alone can cause an individual to experience nausea and potentially withdraw from treatment. Chemotherapy-associated AN is suggested to have a higher incidence in females compared to males.

AN can be represented in a rodent model (conditioned disgust) through the occurrence of conditioned gaping behaviour …

Constructing An In Vitro 3d Model Of The Human Placenta, Michael Zheng

Undergraduate Student Research Internships Conference

The placenta is critical for nurturing fetal growth and development, with dysregulated placentation associated with adverse pregnancy outcomes. The main fetal and maternal placental components consist of trophoblasts and modified endometrial stromal cells known as decidual cells, respectively. Since investigating in vivo placentas in humans through non-invasive methods is challenging, comprehensive in vitro placental models are needed for in-depth studies. However, in vitro 3D placental models that adequately represent and combine fetal and maternal components have been lacking. In this study, we achieved valuable progress in developing an in vitro 3D placental model inclusive of fetal and maternal constituents. We …