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Full-Text Articles in Medicine and Health Sciences

Designing And Testing Of A System For Aerosolization And Recovery Of Viable Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): Theoretical And Engineering Considerations, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks May 2021

Designing And Testing Of A System For Aerosolization And Recovery Of Viable Porcine Reproductive And Respiratory Syndrome Virus (Prrsv): Theoretical And Engineering Considerations, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, Myeongseong Lee, Baitong Chen, William S. Jenks

Agricultural and Biosystems Engineering Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, and ...


Radiation Exposure Associated With Percutaneous Fluoroscopically Guided Lag Screw Fixation For Sacroiliac Luxation In Dogs, Jaron H. Naiman, Eric M. Zellner, Brian L. Petrovsky, Thomas O. Riegel, Elizabeth M. Schmitt, Lingnan Yuan, Jonathan P. Mochel, Karl H. Kraus May 2021

Radiation Exposure Associated With Percutaneous Fluoroscopically Guided Lag Screw Fixation For Sacroiliac Luxation In Dogs, Jaron H. Naiman, Eric M. Zellner, Brian L. Petrovsky, Thomas O. Riegel, Elizabeth M. Schmitt, Lingnan Yuan, Jonathan P. Mochel, Karl H. Kraus

Biomedical Sciences Publications

Objective: To determine radiation exposure to surgical personnel and to evaluate the accuracy of a modified percutaneous lag screw fixation technique for sacroiliac luxation (SIL) under fluoroscopic guidance in dogs.

Study design: Cadaveric experimental study.

Sample population: Seventeen beagle cadavers with iatrogenic SIL.

Methods: Seventeen beagles with iatrogenic SIL underwent reduction and stabilization with 3.5‐mm screws. Hypodermic needles (14 gauge) and fluoroscopy were used to orient two Kirschner wires for temporary stabilization and to guide drilling of glide and pilot holes using cannulated drill bits. Duration of surgery and radiation exposure were recorded. Postoperative computed tomographic evaluation of ...


Short‐Term Outcomes Of 59 Dogs Treated For Ilial Body Fractures With Locking Or Non‐Locking Plates, Brian L. Petrovsky, Taylor Knuth, Cristina Aponte‐Colón, William Hoefle, Karl H. Kraus, Jaron H. Naiman, Lingnan Yuan, Jonathan P. Mochel, Eric Zellner May 2021

Short‐Term Outcomes Of 59 Dogs Treated For Ilial Body Fractures With Locking Or Non‐Locking Plates, Brian L. Petrovsky, Taylor Knuth, Cristina Aponte‐Colón, William Hoefle, Karl H. Kraus, Jaron H. Naiman, Lingnan Yuan, Jonathan P. Mochel, Eric Zellner

Biomedical Sciences Publications

Objective: To determine the influence of plating systems on the clinical outcomes in dogs treated for ilial fractures.

Design: Retrospective study.

Animals: Fifty‐nine dogs (63 hemipelves).

Methods: Radiographs and medical records of dogs with ilial fractures presented to Iowa State University between 2003 and 2019 were reviewed. After fracture reduction, fractures were fixed with a locking plate system (LPS) or non‐locking plate system (NLS). Perioperative, long‐term complications, and follow‐up data were recorded. The frequency of implant failure and pelvic collapse were compared using a logistic and linear regression analysis, respectively. Where the univariate test was statistically ...


Comparison Of Canine And Human Physiological Factors: Understanding Interspecies Differences That Impact Drug Pharmacokinetics, Marilyn N. Martinez, Jonathan P. Mochel, Sibylle Neuhoff, Devendra Pade Apr 2021

Comparison Of Canine And Human Physiological Factors: Understanding Interspecies Differences That Impact Drug Pharmacokinetics, Marilyn N. Martinez, Jonathan P. Mochel, Sibylle Neuhoff, Devendra Pade

Biomedical Sciences Publications

This review is a summary of factors affecting the drug pharmacokinetics (PK) of dogs versus humans. Identifying these interspecies differences can facilitate canine-human PK extrapolations while providing mechanistic insights into species-specific drug in vivo behavior. Such a cross-cutting perspective can be particularly useful when developing therapeutics targeting diseases shared between the two species such as cancer, diabetes, cognitive dysfunction, and inflammatory bowel disease. Furthermore, recognizing these differences also supports a reverse PK extrapolations from humans to dogs. To appreciate the canine-human differences that can affect drug absorption, distribution, metabolism, and elimination, this review provides a comparison of the physiology, drug ...


Longitudinal Piglet Sampling In Commercial Sow Farms Highlights The Challenge Of Prrsv Detection, Marcelo Nunes De Almeida, Cesar A. Corzo, Jeffrey J. Zimmerman, Daniel C. L. Linhares Apr 2021

Longitudinal Piglet Sampling In Commercial Sow Farms Highlights The Challenge Of Prrsv Detection, Marcelo Nunes De Almeida, Cesar A. Corzo, Jeffrey J. Zimmerman, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Background: Processing fluids (PF) and family oral fluids (FOF) are population-based surveillance samples collected from 2- to 5-day-old piglets and due-to-wean piglets, respectively. Although they are described for the surveillance of PRRSV in sows and piglet populations at processing and weaning, there is limited information on their use in commercial herds. This observational study described PRRSV RNA detection over time in PF, FOF, and piglet serum collected from farrowing groups in commercial breeding farms with the objective of achieving robust, practical, and effective PRRSV surveillance protocols. Weekly PF (an aggregate sample of all litters processed in a week from each ...


Swine Disease Reporting System Report #38, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jamie Christopher-Henningson Apr 2021

Swine Disease Reporting System Report #38, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jamie Christopher-Henningson

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...


Pseudorabies (Aujeszky's Disease) Virus Dna Detection In Swine Nasal Swab And Oral Fluid Specimens Using A Gb-Based Real-Time Quantitative Pcr, Ting-Yu Cheng, Alexandra Henao-Diaz, Korakrit Poonsuk, Alexandra Buckley, Albert Van Geelen, Kelly Lager, Karen M. Harmon, Phillip Gauger, Chong Wang, Aruna Ambagala, Jeffrey J. Zimmerman, Luis Giménez-Lirola Apr 2021

Pseudorabies (Aujeszky's Disease) Virus Dna Detection In Swine Nasal Swab And Oral Fluid Specimens Using A Gb-Based Real-Time Quantitative Pcr, Ting-Yu Cheng, Alexandra Henao-Diaz, Korakrit Poonsuk, Alexandra Buckley, Albert Van Geelen, Kelly Lager, Karen M. Harmon, Phillip Gauger, Chong Wang, Aruna Ambagala, Jeffrey J. Zimmerman, Luis Giménez-Lirola

Veterinary Diagnostic and Production Animal Medicine Publications

In this study, the detection of PRV DNA in nasal swab (n = 440) and oral fluid (n = 1,545) samples collected over time from experimentally PRV vaccinated and/or PRV inoculated pigs (n = 40) was comparatively evaluated by real-time PCR. Serum samples (n = 440) were tested by PRV gB/gE blocking ELISAs (Pseudorabies Virus gB Antibody Test Kit and Pseudorabies Virus gpI Antibody Test Kit, IDEXX Laboratories, Inc., Westbrook, ME) to monitor PRV status over time. Following exposure to a gE-deleted modified live vaccine (Ingelvac® Aujeszky MLV, Boehringer Ingelheim, Ridgefield, CT) and/or a wild-type virus (3 CR Ossabaw), PRV ...


Genomics Of Response To Prrsv In Purebred And Crossbred Sows: Antibody Response And Performance Following Natural Infection Versus Vaccination, Leticia P. Sanglard, Felipe M. W. Hickmann, Yijian Huang, Kent A. Gray, Daniel C. L. Linhares, Jack C. M. Dekkers, Megan C. Niederwerder, Rohan L. Fernando, Joseph Braccini Neto, Nick V. L. Serão Mar 2021

Genomics Of Response To Prrsv In Purebred And Crossbred Sows: Antibody Response And Performance Following Natural Infection Versus Vaccination, Leticia P. Sanglard, Felipe M. W. Hickmann, Yijian Huang, Kent A. Gray, Daniel C. L. Linhares, Jack C. M. Dekkers, Megan C. Niederwerder, Rohan L. Fernando, Joseph Braccini Neto, Nick V. L. Serão

Veterinary Diagnostic and Production Animal Medicine Publications

Antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) following a PRRSV-outbreak (S/POutbreak) in a purebred nucleus and following a PRRSV-vaccination (S/PVx) in commercial crossbred herds have been proposed as genetic indicator traits for improved reproductive performance in PRRSV-infected purebred and PRRSV-vaccinated crossbred sows, respectively. In this study, we investigated the genetic relationships of S/POutbreak and S/PVx with performance at the commercial (vaccinated crossbred sows) and nucleus level (non-infected and PRRSV-infected purebred sows), respectively, and tested the effect of previously identified SNP for these indicator traits. Antibody response was ...


Data Standardization Implementation And Applications Within And Among Diagnostic Laboratories: Integrating And Monitoring Enteric Coronaviruses, Giovani Trevisan, Leticia C. M. Linhares, Kent J. Schwartz, Eric R. Burrough, Edison De S. Magalhães, Bret Crim, Poonam Dubey, Rodger G. Main, Phillip C. Gauger, Mary Thurn, Paulo T. F. Lages, Cesar A. Corzo, Jerry Torrison, Jamie Henningson, Eric Herrman, Rob Mcgaughey, Giselle Cino, Jon Greseth, Travis Clement, Jane Christopher-Hennings, Daniel C. L. Linhares Mar 2021

Data Standardization Implementation And Applications Within And Among Diagnostic Laboratories: Integrating And Monitoring Enteric Coronaviruses, Giovani Trevisan, Leticia C. M. Linhares, Kent J. Schwartz, Eric R. Burrough, Edison De S. Magalhães, Bret Crim, Poonam Dubey, Rodger G. Main, Phillip C. Gauger, Mary Thurn, Paulo T. F. Lages, Cesar A. Corzo, Jerry Torrison, Jamie Henningson, Eric Herrman, Rob Mcgaughey, Giselle Cino, Jon Greseth, Travis Clement, Jane Christopher-Hennings, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Every day, thousands of samples from diverse populations of animals are submitted to veterinary diagnostic laboratories (VDLs) for testing. Each VDL has its own laboratory information management system (LIMS), with processes and procedures to capture submission information, perform laboratory tests, define the boundaries of test results (i.e., positive or negative), and report results, in addition to internal business and accounting applications. Enormous quantities of data are accumulated and stored within VDL LIMSs. There is a need for platforms that allow VDLs to exchange and share portions of laboratory data using standardized, reliable, and sustainable information technology processes. Here we ...


Mitigation Of Airborne Prrsv Transmission With Uv Light Treatment: Proof-Of-Concept, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, William S. Jenks, Myeongseong Lee, Baitong Chen, Steven J. Hoff Mar 2021

Mitigation Of Airborne Prrsv Transmission With Uv Light Treatment: Proof-Of-Concept, Peiyang Li, Jacek A. Koziel, Jeffrey J. Zimmerman, Jianqiang Zhang, Ting-Yu Cheng, Wannarat Yim-Im, William S. Jenks, Myeongseong Lee, Baitong Chen, Steven J. Hoff

Agricultural and Biosystems Engineering Publications

Proper treatment of infectious air could potentially mitigate the spread of airborne viruses such as porcine reproductive and respiratory syndrome virus (PRRSV). The objective of this research is to test the effectiveness of ultraviolet (UV) in inactivating aerosolized PRRSV, specifically, four UV lamps, UV-A (365 nm, both fluorescent and LED-based), “excimer” UV-C (222 nm), and germicidal UV-C (254 nm), were tested. The two UV-C lamps effectively irradiated fast-moving PRRSV aerosols with short treatment times (<2 s). One-stage and two-stage UV inactivation models estimated the UV doses needed for target percentage (%) reductions on PRRSV titer. UV-C (254 nm) dose needed for 3-log (99.9%) reduction was 0.521 and 0.0943 mJ/cm2, respectively, based on one-stage and two-stage models. An order of magnitude lower UV-C (222 nm) doses were needed for a 3-log reduction, i.e., 0 ...


Vaginal Microbiome And Serum Metabolite Differences In Late Gestation Commercial Sows At Risk For Pelvic Organ Prolapse, Zoë E. Kiefer, Lucas R. Koester, Lucas Showman, Jamie M. Studer, Amanda L. Chipman, Aileen F. Keating, Stephan Schmitz-Esser, Jason W. Ross Mar 2021

Vaginal Microbiome And Serum Metabolite Differences In Late Gestation Commercial Sows At Risk For Pelvic Organ Prolapse, Zoë E. Kiefer, Lucas R. Koester, Lucas Showman, Jamie M. Studer, Amanda L. Chipman, Aileen F. Keating, Stephan Schmitz-Esser, Jason W. Ross

Animal Science Publications

Sow mortality attributable to pelvic organ prolapse (POP) has increased in the U.S. swine industry and continues to worsen. Two main objectives of this study were, (1) to develop a perineal scoring system that can be correlated with POP risk, and (2) identify POP risk-associated biological factors. To assess POP risk during late gestation, sows (n = 213) were scored using a newly developed perineal scoring (PS) system. Sows scored as PS1 (low), PS2 (moderate), or PS3 (high) based on POP risk. Subsequently, 1.5, 0.8, and 23.1% of sows scored PS1, PS2, or PS3, respectively, experienced POP ...


Distribution Of Crispr Types In Fluoroquinolone-Resistant Campylobacter Jejuni Isolates, Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin Mar 2021

Distribution Of Crispr Types In Fluoroquinolone-Resistant Campylobacter Jejuni Isolates, Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin

Veterinary Microbiology and Preventive Medicine Publications

To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the ...


Visualization And Application Of Disease Diagnosis Codes For Population Health Management Using Porcine Diseases As A Model, Giovani Trevisan, Kent J. Schwartz, Eric R. Burrough, Bailey Arruda, Rachel J. Derscheid, Michael C. Rahe, Edison De Souza Magalhães, Marcelo N. Almeida, Rodger G. Main, Daniel C. L. Linhares Mar 2021

Visualization And Application Of Disease Diagnosis Codes For Population Health Management Using Porcine Diseases As A Model, Giovani Trevisan, Kent J. Schwartz, Eric R. Burrough, Bailey Arruda, Rachel J. Derscheid, Michael C. Rahe, Edison De Souza Magalhães, Marcelo N. Almeida, Rodger G. Main, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Accurate and timely results of diagnostic investigations and laboratory testing guide clinical interventions for the continuous improvement of animal health and welfare. Infectious diseases can severely limit the health, welfare, and productivity of populations of animals. Livestock veterinarians submit thousands of samples daily to veterinary diagnostic laboratories (VDLs) for disease diagnosis, pathogen monitoring, and surveillance. Individual diagnostic laboratory reports are immediately useful; however, aggregated historical laboratory data are increasingly valued by clinicians and decision-makers to identify changes in the health status of various animal populations over time and geographical space. The value of this historical information is enhanced by visualization ...


Systemic Tissue And Cellular Disruption From Sars-Cov-2 Infection Revealed In Covid- 19 Autopsies And Spatial Omics Tissue Maps, Jiwoon Park, Jonathan Foox, Tyler Hether, Afshin Beheshti, Amanda Saravia-Butler, Urminder Singh, Eve Syrkin Wurtele, Et Al. Mar 2021

Systemic Tissue And Cellular Disruption From Sars-Cov-2 Infection Revealed In Covid- 19 Autopsies And Spatial Omics Tissue Maps, Jiwoon Park, Jonathan Foox, Tyler Hether, Afshin Beheshti, Amanda Saravia-Butler, Urminder Singh, Eve Syrkin Wurtele, Et Al.

Genetics, Development and Cell Biology Publications

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus has infected over 115 million people and caused over 2.5 million deaths worldwide. Yet, the molecular mechanisms underlying the clinical manifestations of COVID-19, as well as what distinguishes them from common seasonal influenza virus and other lung injury states such as Acute Respiratory Distress Syndrome (ARDS), remains poorly understood. To address these challenges, we combined transcriptional profiling of 646 clinical nasopharyngeal swabs and 39 patient autopsy tissues, matched with spatial protein and expression profiling (GeoMx) across 357 tissue sections. These results define both body-wide and tissue-specific (heart, liver, lung, kidney ...


Acute Corneal Hydrops Of Presumed Traumatic Origin: An Uncontrolled Case Series (Three Horses), Louise M. O'Leary, Lionel Sebbag, L. M. Moody, K. A. Diehl, Rachel A. Allbaugh Mar 2021

Acute Corneal Hydrops Of Presumed Traumatic Origin: An Uncontrolled Case Series (Three Horses), Louise M. O'Leary, Lionel Sebbag, L. M. Moody, K. A. Diehl, Rachel A. Allbaugh

Veterinary Clinical Sciences Publications

This case series describes acute corneal hydrops in three young horses. Due to similarities in the clinical appearance and progression of the disease with that which is reported in humans and cats with acute corneal hydrops, traumatic Descemet’s membrane rupture was suspected to be the underlying aetiology in these equine cases. The horses presented with acute severe corneal oedema with intrastromal bullae formation and anterior bulging of the corneal contour. Focal posterior corneal changes were also seen in two of three cases. Mild anterior uveitis was also present. Other causes of corneal oedema (e.g. glaucoma) were ruled out ...


Swine Disease Reporting System Report #37, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jamie Christopher-Henningson Mar 2021

Swine Disease Reporting System Report #37, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jamie Christopher-Henningson

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...


Effect Of Prrsv Stability On Productive Parameters In Breeding Herds Of A Swine Large Integrated Group In Spain, D. Torrents, J. Miranda, Phillip C. Gauger, Alejandro Ramirez, Daniel C. L. Linhares Feb 2021

Effect Of Prrsv Stability On Productive Parameters In Breeding Herds Of A Swine Large Integrated Group In Spain, D. Torrents, J. Miranda, Phillip C. Gauger, Alejandro Ramirez, Daniel C. L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Background In breeding herds, porcine reproductive and respiratory syndrome (PRRS) clinically manifests as increased abortions, number of stillbirths, and pre-weaning mortality, and as a direct consequence, results in a decrease of the number of piglets weaned per sow per year. Breeding farm classification according the PRRS virus (PRRSV) status (unstable or stable) is a key control strategy for this disease. The aim of this study was to evaluate the production improvement related to achieving a PRRSV stable status in breeding herds in Spain. For this purpose, epidemiological and productivity data were collected from a systematic PRRSV monitoring program in 35 ...


Complete Genome Sequence Of Campylobacter Hepaticus Usa52, Associated With Chicken Spotty Liver Disease, Zuowei Wu, Orhan Sahin, Qijing Zhang Feb 2021

Complete Genome Sequence Of Campylobacter Hepaticus Usa52, Associated With Chicken Spotty Liver Disease, Zuowei Wu, Orhan Sahin, Qijing Zhang

Veterinary Microbiology and Preventive Medicine Publications

Campylobacter hepaticus was recently identified as the etiological agent of chicken spotty liver disease. Here, we report the complete genome sequence of C. hepaticus strain USA52 from the United States. The genome comprises a chromosome of 1,509,100 bp with an average GC content of 28.02%.


Collagen Has A Unique Sec24 Preference For Efficient Export From The Endoplasmic Reticulum, Chung-Ling Lu, Jacob Cain, Jon Brudvig, Steven Ortmeier, Simeon A. Boyadjiev, Jill M. Weimer, Jinoh Kim Feb 2021

Collagen Has A Unique Sec24 Preference For Efficient Export From The Endoplasmic Reticulum, Chung-Ling Lu, Jacob Cain, Jon Brudvig, Steven Ortmeier, Simeon A. Boyadjiev, Jill M. Weimer, Jinoh Kim

Biomedical Sciences Publications

Procollagen requires COPII coat proteins for export from the endoplasmic reticulum (ER). SEC24 is the major component of the COPII proteins that selects cargo during COPII vesicle assembly. There are four paralogs (A to D) of SEC24 in mammals, which are classified into two subgroups. Pathological mutations in SEC24D cause osteogenesis imperfecta with craniofacial dysplasia in humans and sec24d mutant fish also recapitulate this phenotypes. Consistent with the skeletal phenotypes, the secretion of collagen was severely defective in mutant fish, emphasizing the importance of SEC24D in collagen secretion. However, SEC24D patient derived fibroblasts show only a mild secretion phenotype, suggesting ...


Gene Expression In Tonsils In Swine Following Infection With Porcine Reproductive And Respiratory Syndrome Virus, Qian Dong, Joan K. Lunney, Kyu-Sang Lim, Yet Nguyen, Andrew S. Hess, Hamid Beiki, Raymond R. R. Rowland, Kristen Walker, James M. Reecy, Christopher K. Tuggle, Jack C. M. Dekkers Feb 2021

Gene Expression In Tonsils In Swine Following Infection With Porcine Reproductive And Respiratory Syndrome Virus, Qian Dong, Joan K. Lunney, Kyu-Sang Lim, Yet Nguyen, Andrew S. Hess, Hamid Beiki, Raymond R. R. Rowland, Kristen Walker, James M. Reecy, Christopher K. Tuggle, Jack C. M. Dekkers

Animal Science Publications

Background: Porcine reproductive and respiratory syndrome (PRRS) is a threat to pig production worldwide. Our objective was to understand mechanisms of persistence of PRRS virus (PRRSV) in tonsil. Transcriptome data from tonsil samples collected at 42 days post infection (dpi) were generated by RNA-seq and NanoString on 51 pigs that were selected to contrast the two PRRSV isolates used, NVSL and KS06, high and low tonsil viral level at 42 dpi, and the favorable and unfavorable genotypes at a genetic marker (WUR) for the putative PRRSV resistance gene GBP5.

Results: The number of differentially expressed genes (DEGs) differed markedly between ...


Nudivirus Sequences Identified From The Southern And Western Corn Rootworms (Coleoptera: Chrysomelidae), Sijun Liu, Thomas W. Sappington, Brad S. Coates, Bryony C. Bonning Feb 2021

Nudivirus Sequences Identified From The Southern And Western Corn Rootworms (Coleoptera: Chrysomelidae), Sijun Liu, Thomas W. Sappington, Brad S. Coates, Bryony C. Bonning

Entomology Publications

Analysis of pooled genomic short read sequence data revealed the presence of nudivirus-derived sequences from U.S. populations of both southern corn rootworm (SCR, Diabrotica undecimpunctata howardi Barber) and western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte). A near complete nudivirus genome sequence was assembled from sequence data for an SCR population with relatively high viral titers. A total of 147,179 bp was assembled from five contigs that collectively encode 109 putative open reading frames (ORFs) including 20 nudivirus core genes. In contrast, genome sequence recovery was incomplete for a second nudivirus from WCR, although sequences derived from this ...


Evaluation Of Individual And Ensemble Probabilistic Forecasts Of Covid-19 Mortality In The Us, Estee Y. Cramer, Velma K. Lopez, Jarad Niemi, Glover E. George, Jeffrey C. Cegan, Ian D. Dettwiller, William P. England, Matthew W. Farthing, Robert H. Hunter, Brandon Lafferty, Igor Linkov, Michael L. Mayo, Matthew D. Parno, Michael A. Rowland, Benjamin D. Trump, Lily Wang, Lei Gao, Zhiling Gu, Myungjin Kim, Yueying Wang, Jo W. Walker, Rachel B. Slayton, Michael Johansson, Matthew Biggerstaff, Et Al. Feb 2021

Evaluation Of Individual And Ensemble Probabilistic Forecasts Of Covid-19 Mortality In The Us, Estee Y. Cramer, Velma K. Lopez, Jarad Niemi, Glover E. George, Jeffrey C. Cegan, Ian D. Dettwiller, William P. England, Matthew W. Farthing, Robert H. Hunter, Brandon Lafferty, Igor Linkov, Michael L. Mayo, Matthew D. Parno, Michael A. Rowland, Benjamin D. Trump, Lily Wang, Lei Gao, Zhiling Gu, Myungjin Kim, Yueying Wang, Jo W. Walker, Rachel B. Slayton, Michael Johansson, Matthew Biggerstaff, Et Al.

Statistics Publications

Short-term probabilistic forecasts of the trajectory of the COVID-19 pandemic in the United States have served as a visible and important communication channel between the scientific modeling community and both the general public and decision-makers. Forecasting models provide specific, quantitative, and evaluable predictions that inform short-term decisions such as healthcare staffing needs, school closures, and allocation of medical supplies. In 2020, the COVID-19 Forecast Hub (https://covid19forecasthub.org/) collected, disseminated, and synthesized hundreds of thousands of specific predictions from more than 50 different academic, industry, and independent research groups. This manuscript systematically evaluates 23 models that regularly submitted forecasts of ...


Swine Disease Reporting System Report #36, Giovani Trevisan, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Daniel Linhares, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Gretseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings Feb 2021

Swine Disease Reporting System Report #36, Giovani Trevisan, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Ryan Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Daniel Linhares, Mary Thurn, Paulo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Gretseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...


Advances In Mutation Detection Using Loop-Mediated Isothermal Amplification, Marcelino Varona, Jared L. Anderson Jan 2021

Advances In Mutation Detection Using Loop-Mediated Isothermal Amplification, Marcelino Varona, Jared L. Anderson

Chemistry Publications

Detection of mutations and single-nucleotide polymorphisms is highly important for diagnostic applications. Loop-mediated isothermal amplification (LAMP) is a powerful technique for the rapid and sensitive detection of nucleic acids. However, LAMP traditionally does not possess the ability to resolve single-nucleotide differences within the target sequence. Because of its speed and isothermal nature, LAMP is ideally suited for point-of-care applications in resource-limited settings. Recently, different approaches have been developed and applied to enable single-nucleotide differentiation within target sequences. This Mini-Review highlights advancements in mutation detection using LAMP. Methods involving primer design and modification to enable sequence differentiation are discussed. In addition ...


Xylanase Modulates The Microbiota Of Ileal Mucosa And Digesta Of Pigs Fed Corn-Based Arabinoxylans Likely Through Both A Stimbiotic And Prebiotic Mechanism, Amy L. Petry, John F. Patience, Lucas R. Koester, Nichole F. Huntley, Michael R. Bedford, Stephan Schmitz-Esser Jan 2021

Xylanase Modulates The Microbiota Of Ileal Mucosa And Digesta Of Pigs Fed Corn-Based Arabinoxylans Likely Through Both A Stimbiotic And Prebiotic Mechanism, Amy L. Petry, John F. Patience, Lucas R. Koester, Nichole F. Huntley, Michael R. Bedford, Stephan Schmitz-Esser

Animal Science Publications

The experimental objective was to characterize the impact of insoluble corn-based fiber, xylanase, and an arabinoxylan-oligosaccharide on ileal digesta and mucosa microbiome of pigs. Three replicates of 20 gilts were blocked by initial body weight, individually-housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF), a 30% corn bran high-fiber control (HF), HF+100 mg/kg xylanase (HF+XY), and HF+50 mg/kg arabinoxylan oligosaccharide (HF+AX). Gilts were fed their respective treatments for 46 days. On day 46, pigs were euthanized and ileal digesta and mucosa were collected. The V4 region of the 16S rRNA ...


Letter To The Editor Regarding “Efficacy Of Adding Ramipril (Vasotop) To The Combination Of Furosemide (Lasix) And Pimobendan (Vetmedin) In Dogs With Mitral Valve Degeneration: The Valve Trial”, Clarke Atkins, Bruce Keene, Teresa C. Defrancesco, Sandra Tou, Valérie Chetboul, Étienne Côté, Stephen Ettinger, Philip R. Fox, Robert L. Hamlin, Jonathan P. Mochel, Jean‐Louis Pouchelon, Rebecca L. Stepien Jan 2021

Letter To The Editor Regarding “Efficacy Of Adding Ramipril (Vasotop) To The Combination Of Furosemide (Lasix) And Pimobendan (Vetmedin) In Dogs With Mitral Valve Degeneration: The Valve Trial”, Clarke Atkins, Bruce Keene, Teresa C. Defrancesco, Sandra Tou, Valérie Chetboul, Étienne Côté, Stephen Ettinger, Philip R. Fox, Robert L. Hamlin, Jonathan P. Mochel, Jean‐Louis Pouchelon, Rebecca L. Stepien

Biomedical Sciences Publications

The manuscript entitled Efficacy of adding ramipril (VAsotop) to the combination of furosemide (Lasix) and pimobendan (VEtmedin) in dogs with mitral valve degeneration: The VALVE trial1 reports a study which sought to answer the question “could pimobendan be all that is needed beyond loop diuretics to manage congestive heart failure (CHF) in myxomatous mitral valve disease (MMVD)?” This was done by prospectively comparing the efficacy of pimobendan + ramipril + furosemide (triple treatment) to pimobendan + furosemide (double treatment) to treat new‐onset CHF caused by MMVD.


Altered Corneal Innervation And Ocular Surface Homeostasis In Fhv-1-Exposed Cats: A Preliminary Study Suggesting Metaherpetic Disease, Lionel Sebbag, Sara M. Thomasy, Adriana Leland, Madison Mukai, Soohyun Kim, David J. Maggs Jan 2021

Altered Corneal Innervation And Ocular Surface Homeostasis In Fhv-1-Exposed Cats: A Preliminary Study Suggesting Metaherpetic Disease, Lionel Sebbag, Sara M. Thomasy, Adriana Leland, Madison Mukai, Soohyun Kim, David J. Maggs

Veterinary Clinical Sciences Publications

Metaherpetic disease is recognized in humans affected by herpes simplex virus-1 but is not reported in cats affected by feline herpesvirus-1 (FHV-1) despite the high prevalence of herpetic disease in this species and strong similarities in viral biology between alphaherpesviruses of humans and cats. This preliminary work evaluated cats naïve to FHV-1 (n = 9 cats, 18 eyes; control population) and cats naturally exposed to FHV-1 (n = 4 cats, 7 eyes), as confirmed by serologic testing and review of medical records. Antemortem assessment included clinical scoring, blink rate, corneal aesthesiometry, tear film breakup time (TFBUT), and Schirmer tear test-1 (STT-1) with ...


Measuring Femoral Neck Loads In Healthy Young And Older Adults During Stair Ascent And Descent, Chen Deng, Jason C. Gillette, Timothy R. Derrick Jan 2021

Measuring Femoral Neck Loads In Healthy Young And Older Adults During Stair Ascent And Descent, Chen Deng, Jason C. Gillette, Timothy R. Derrick

Kinesiology Publications

Understanding the hip loading environment for daily activities is useful for hip fracture prevention, rehabilitation, and the design of osteogenic exercises. Seventeen older adults (50–70 yrs) and twenty young adults (18–30 yrs) were recruited. A rigid body model combined with a musculoskeletal model was used to estimate lower extremity loading. An elliptical cross-section model of the femoral neck was used to estimate femoral neck stress during stair ascent and descent. Two peaks were identified in the stress curves, corresponding to the peaks in the vertical ground reaction force. During stair ascent, significantly higher tension on the superior femoral ...


Learning Innovation And Service-Learning In Medical Apparel Development Of Entrepreneurship: Face-Covering, Changhyun (Lyon) Nam, Srikant Manchiraju, Eulanda A. Sanders Jan 2021

Learning Innovation And Service-Learning In Medical Apparel Development Of Entrepreneurship: Face-Covering, Changhyun (Lyon) Nam, Srikant Manchiraju, Eulanda A. Sanders

Apparel, Events and Hospitality Management Conference Proceedings and Presentations

The Medical Apparel Development of Entrepreneurship course helps students: (a) understand fundamental product development concept and the process, (b) implement medical apparel development of entrepreneurship incorporating 3D printing, and (c) contribute to local community service centers for a service-learning practice task. The overall design concept and process will be useful in course content, including seven face-covering steps during the sixteenweek course. It is imperative for academia and industry to collaborate and contribute to finding an optimal face-covering prototype, created from suitable materials and elastics, which is washable, reusable, and hygienic better to understand the unique and straightforward pattern and design ...


Swine Disease Reporting System Report #35, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paolo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings Jan 2021

Swine Disease Reporting System Report #35, Giovani Trevisan, Daniel Linhares, Edison Magalhaes, Leticia C. M. Linhares, Bret Crim, Poonam Dubey, Kent J. Schwartz, Eric Burrough, Phillip Charles Gauger, Pablo Pineyro, Christopher Siepker, Rodger G. Main, Mary Thurn, Paolo Lages, Cesar Corzo, Jerry Torrison, Rob Mcgaughey, Eric Herrman, Roman Pogranichniy, Rachel Palinski, Jamie Henningson, Jon Greseth, Darren Kersey, Travis Clement, Jane Christopher-Hennings

Swine Disease Reporting System

What is the Swine Disease Reporting System (SDRS)? SDRS includes multiple projects that aggregate data from participating veterinary diagnostic laboratories (VDLs) in the United States of America (USA), and reports the major findings to the swine industry. Our goal is to share information on endemic and emerging diseases affecting the swine population in the USA, assisting veterinarians and producers in making informed decisions on disease prevention, detection, and management.

After aggregating information from participating VDLs and summarizing the data, we ask the input of our advisory group, which consists of veterinarians and producers across the USA swine industry. The intent ...