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Full-Text Articles in Medicine and Health Sciences
Nanostructures, Concentrations And Energies: An Ideal Equation To Extend Therapeutic Efficiency On Radioresistant 9l Tumour Cells Using Ta2o5 Ceramic Nanostructured Particles, Ryan Brown, Stephanie Corde, Sianne Oktaria, Konstantin K. Konstantinov, Anatoly B. Rosenfeld, Michael L. F Lerch, Moeava Tehei
Nanostructures, Concentrations And Energies: An Ideal Equation To Extend Therapeutic Efficiency On Radioresistant 9l Tumour Cells Using Ta2o5 Ceramic Nanostructured Particles, Ryan Brown, Stephanie Corde, Sianne Oktaria, Konstantin K. Konstantinov, Anatoly B. Rosenfeld, Michael L. F Lerch, Moeava Tehei
Illawarra Health and Medical Research Institute
This work presents an in-depth analysis into the dependencies of radiosensitisation on X-ray beam energy, particle morphology and particle concentration for Ta2O5 nanostructured particles. A maximum sensitisation enhancement ratio of 1.46 was attained with irradiation of a 10 MV x-ray photon beam on 9L cells exposed to the less aggregated form of NSPs at 500 μg/mL-1. A significant increase in sensitisation of 30% was noted at 150 kVp for irradiation of the less aggregated form of tantalum pentoxide NSPs compared to its more agglomerated counterpart. Interestingly, no differences in sensitisation were observed between 50 and …
Berberine Alleviates Olanzapine-Induced Adipogenesis Via The Ampkα-Srebp Pathway In 3t3-L1 Cells, Yan Jie Li, Xiaomin Zhao, Xiyu Feng, Xuemei Liu, Chao Deng, Chang-Hua Hu
Berberine Alleviates Olanzapine-Induced Adipogenesis Via The Ampkα-Srebp Pathway In 3t3-L1 Cells, Yan Jie Li, Xiaomin Zhao, Xiyu Feng, Xuemei Liu, Chao Deng, Chang-Hua Hu
Illawarra Health and Medical Research Institute
The aim of this study was to investigate the mechanisms underlying the inhibitory effects of berberine (BBR) on olanzapine (OLZ)-induced adipogenesis in a well-replicated 3T3-L1 cell model. Oil-Red-O (ORO) staining showed that BBR significantly decreased OLZ-induced adipogenesis. Co-treatment with OLZ and BBR decreased the accumulation of triglyceride (TG) and total cholesterol (TC) by 55.58% ± 3.65% and 49.84% ± 8.31%, respectively, in 3T3-L1 adipocytes accompanied by reduced expression of Sterol regulatory element binding proteins 1 (SREBP1), fatty acid synthase (FAS), peroxisome proliferator activated receptorγ (PPARγ), SREBP2, low-density lipoprotein receptor (LDLR), and hydroxymethylglutaryl-coenzyme A reductase (HMGR) genes compared with OLZ alone. …
First Proof Of Bismuth Oxide Nanoparticles As Efficient Radiosensitisers On Highly Radioresistant Cancer Cells, Callum Stewart, Konstantin K. Konstantinov, Sally Mckinnon, Susanna Guatelli, Michael L. F Lerch, Anatoly B. Rosenfeld, Moeava Tehei, Stephanie Corde
First Proof Of Bismuth Oxide Nanoparticles As Efficient Radiosensitisers On Highly Radioresistant Cancer Cells, Callum Stewart, Konstantin K. Konstantinov, Sally Mckinnon, Susanna Guatelli, Michael L. F Lerch, Anatoly B. Rosenfeld, Moeava Tehei, Stephanie Corde
Illawarra Health and Medical Research Institute
This study provides the first proof of the novel application of bismuth oxide as a radiosensitiser. It was shown that on the highly radioresistant 9L gliosarcoma cell line, bismuth oxide nanoparticles sensitise to both kilovoltage (kVp) or megavoltage (MV) X-rays radiation. 9L cells were exposed to a concentration of 50 μg.mL−1 of nanoparticle before irradiation at 125 kVp and 10 MV. Sensitisation enhancement ratios of 1.48 and 1.25 for 125 kVp and 10 MV were obtained in vitro, respectively. The radiation enhancement of the nanoparticles is postulated to be a combination of the high Z nature of the …
The Key Regulatory Roles Of The Pi3k/Akt Signaling Pathway In The Functionalities Of Mesenchymal Stem Cells And Applications In Tissue Regeneration, Jiezhong Chen, Ross Crawford, Chen Chen, Yin Xiao
The Key Regulatory Roles Of The Pi3k/Akt Signaling Pathway In The Functionalities Of Mesenchymal Stem Cells And Applications In Tissue Regeneration, Jiezhong Chen, Ross Crawford, Chen Chen, Yin Xiao
Illawarra Health and Medical Research Institute
Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and have been widely used in tissue engineering application. In tissue engineering, a scaffold, MSCs and growth factors are used as essential components and their interactions have been regarded to be important for regeneration of tissues. A critical problem for MSCs in tissue engineering is their low survival ability and functionality. Most MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity …
Tnf-Α Promotes Ifn-Γ-Induced Cd40 Expression And Antigen Process In Myb-Transformed Hematological Cells, Wenyi Gu, Jiezhong Chen, Lei Yang, Kong-Nan Zhao
Tnf-Α Promotes Ifn-Γ-Induced Cd40 Expression And Antigen Process In Myb-Transformed Hematological Cells, Wenyi Gu, Jiezhong Chen, Lei Yang, Kong-Nan Zhao
Illawarra Health and Medical Research Institute
Tumour necrosis factor-α, interferon-γ and interleukin-4 are critical cytokines in regulating the immune responses against infections and tumours. In this study, we investigated the effects of three cytokines on CD40 expression in Myb-transformed hematological cells and their regulatory roles in promoting these cells into dendritic cells. We observed that both interleukin-4 and interferon-γ increased CD40 expression in these hematological cells in a dose-dependent manner, although the concentration required for interleukin-4 was significantly higher than that for interferon-γ. We found that tumour necrosis factor-α promoted CD40 expression induced by interferon-γ, but not by interleukin-4. Our data showed that tumour necrosis factor-α …
Functional Expression Of The Damage-Associated Molecular Pattern Receptor P2x7 On Canine Kidney Epithelial Cells, Iman Jalilian, Mari Spildrejorde, Aine Seavers, Belinda L. Curtis, Jason D. Mcarthur, Ronald Sluyter
Functional Expression Of The Damage-Associated Molecular Pattern Receptor P2x7 On Canine Kidney Epithelial Cells, Iman Jalilian, Mari Spildrejorde, Aine Seavers, Belinda L. Curtis, Jason D. Mcarthur, Ronald Sluyter
Illawarra Health and Medical Research Institute
Epithelial cells are important in inflammation and immunity. In this study, we examined if Madin-Darby canine kidney (MDCK) epithelial cells express functional P2X7 receptors, which bind the damage-associated molecular pattern extracellular adenosine 5'-triphosphate (ATP). Reverse transcription (RT)-PCR and immunoblotting revealed the expression of P2X7 in MDCK cells. A flow cytometric assay demonstrated that ATP or 2'(3')-O-(4-benzoylbenzoyl)ATP induced ethidium(+) uptake into MDCK cells, and that this process was impaired by the P2X7 antagonists KN-62 and A438079. RT-PCR also demonstrated the presence of Toll-like receptor 4, NALP3, caspase-1, interleukin-1β and interleukin-18 in MDCK cells, as well as in positive control LPS-primed canine …