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Full-Text Articles in Medicine and Health Sciences

Pharmacokinetics Of Intramuscular Maropitant In Pigs (Sus Scrofa Domesticus), Joseph Smith, Jessica E. Gebert, Lisa S. Ebner, Kailee O. Bennett, Rebecca J. Collins, Chiara E. Hampton, Stephanie A. Kleine, Pierre-Yves Mulon, Christopher K. Smith, Reza Seddighi, Genevieve Bussieres, Jonathan P. Mochel, Healther K. Knych Mar 2023

Pharmacokinetics Of Intramuscular Maropitant In Pigs (Sus Scrofa Domesticus), Joseph Smith, Jessica E. Gebert, Lisa S. Ebner, Kailee O. Bennett, Rebecca J. Collins, Chiara E. Hampton, Stephanie A. Kleine, Pierre-Yves Mulon, Christopher K. Smith, Reza Seddighi, Genevieve Bussieres, Jonathan P. Mochel, Healther K. Knych

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Pigs are at risk of vomiting from medical conditions as well as the emetic side effects of drugs administered for peri-operative manipulations, but there is a lack of pharmacokinetic data for potential anti-emetic therapies, such as maropitant, in this species. The main objective of this study was to estimate plasma pharmacokinetic parameters for maropitant in pigs after a single intramuscular (IM) administration dosed at 1.0 mg/ kg. A secondary objective was to estimate pilot pharmacokinetic parameters in pigs after oral (PO) administration at 2.0 mg/kg. Maropitant was administered to six commercial pigs at a dose of 1.0 mg/kg IM. Plasma …


Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmina Gallardo, Jordan T. Gebhardt, Jurgen A. Richt Jan 2021

Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmina Gallardo, Jordan T. Gebhardt, Jurgen A. Richt

Kansas Agricultural Experiment Station Research Reports

As the United States maintains trade with countries where African swine fever virus (ASFV) is endemic, it is critical to have methods that can detect and mitigate the risk of ASFV in potentially contaminated feed or ingredients. Therefore, the objectives of this study were to 1) evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and 2) determine if a feed sampling method could identify ASFV following experimental inoculation. Batches of feed were manufactured in a BSL-3Ag room at Kansas State University’s Biosafety Research Institute in Manhattan, KS. First, the pilot feed manufacturing system …


Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmen Gallardo, Jordan T. Gebhardt, Jurgen A. Richt Jan 2021

Prevalence And Distribution Of African Swine Fever Virus In Swine Feed After Mixing And Feed Batch Sequencing, C. Grace Elijah, Jessie D. Trujillo, Cassandra K. Jones, Taeyong Kwon, Charles R. Stark, Konner R. Cool, Chad B. Paulk, Natasha N. Gaudreault, Jason C. Woodworth, Igor Morozov, Carmen Gallardo, Jordan T. Gebhardt, Jurgen A. Richt

Kansas Agricultural Experiment Station Research Reports

As the United States maintains trade with countries where African swine fever virus (ASFV) is endemic, it is critical to have methods that can detect and mitigate the risk of ASFV in potentially contaminated feed or ingredients. Therefore, the objectives of this study were to 1) evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and 2) determine if a feed sampling method could identify ASFV following experimental inoculation. Batches of feed were manufactured in a BSL-3Ag room at Kansas State University’s Biosafety Research Institute in Manhattan, KS. First, the pilot feed manufacturing system …


Multi-Level Movement Response Of Invasive Wild Pigs (Sus Scrofa) To Removal, Guillaume Bastille-Rousseau, Peter E. Schlichting, David A. Keiter, Joshua B. Smith, John C. Kilgo, George Wittemyer, Kurt C. Vercauteren, James C. Beasley, Kim M. Pepin Jan 2021

Multi-Level Movement Response Of Invasive Wild Pigs (Sus Scrofa) To Removal, Guillaume Bastille-Rousseau, Peter E. Schlichting, David A. Keiter, Joshua B. Smith, John C. Kilgo, George Wittemyer, Kurt C. Vercauteren, James C. Beasley, Kim M. Pepin

USDA Wildlife Services: Staff Publications

BACKGROUND: Lethal removal of invasive species, such as wild pigs (Sus scrofa), is often the most efficient approach for reducing their negative impacts. Wild pigs are one of the most widespread and destructive invasive mammals in the USA. Lethal management techniques are a key approach for wild pigs and can alter wild pig spatial behavior, but it is unclear how wild pigs respond to the most common removal technique, trapping.We investigated the spatial behavior of wild pigs following intensive removal of conspecifics via trapping at three sites within the Savannah River Site, SC, USA. We evaluated changes in …


A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber Jan 2021

A Framework For Surveillance Of Emerging Pathogens At The Human-Animal Interface: Pigs And Coronaviruses As A Case Study, Kim M. Pepin, Ryan S. Miller, Mark Q. Wilber

USDA Wildlife Services: Staff Publications

Pigs (Sus scrofa) may be important surveillance targets for risk assessment and risk-based control planning against emerging zoonoses. Pigs have high contact rates with humans and other animals, transmit similar pathogens as humans including CoVs, and serve as reservoirs and intermediate hosts for notable human pandemics. Wild and domestic pigs both interface with humans and each other but have unique ecologies that demand different surveillance strategies. Three fundamental questions shape any surveillance program: where, when, and how can surveillance be conducted to optimize the surveillance objective? Using theory of mechanisms of zoonotic spillover and data on risk factors, …


A Micromorphometric Study On The Common Bile Duct And Major Duodenal Papilla Of Thedomestic Swine, Ivaylo Stefanov Stefanov Jan 2021

A Micromorphometric Study On The Common Bile Duct And Major Duodenal Papilla Of Thedomestic Swine, Ivaylo Stefanov Stefanov

Turkish Journal of Veterinary & Animal Sciences

The aim of the current study was to perform a comparative micromorphometric study on the thickness of mucosa, fibromuscular layer, glandular zone, serosa, and subserous layer, as well as of the entire wall of extra- and intramural parts of the porcine common bile duct in immature and mature animals. This micromorphometric study also evaluated the height and width of the lining epithelium, glandular epithelium, and goblet cells in 2-month, 6-month, and 3-year old male pigs. It was found that significant age-dependent differences in the studied parameters exist. Some differences in the studied parameters were also detected between different parts of …


Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández Dec 2020

Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández

Graduate Theses and Dissertations

To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …


Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little Jan 2019

Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …


Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth Jan 2019

Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Medium chain fatty acid (MCFA) application has been identified as a promising strategy to decrease viral pathogen transmission in swine feed. Four experiments were conducted to: 1) determine if MCFAs are effective when applied to feed both prior to and after porcine epidemic diarrhea virus (PEDV) inoculation measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 2) evaluate the effects of varying amounts and combinations of MCFA measured by qRT-PCR, and 3) evaluate selected MCFA treatments in a bioassay. In Exp. 1, treatments were arranged in a 2 × 2 + 1 factorial with the main effects of chemical treatment …


Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron Dec 2018

Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron

The Thai Journal of Veterinary Medicine

Fibrin glue has been widely used for controlling hemorrhage and sealing tissue in surgery. The purpose of this study was to evaluate the hemostatic efficacy of sheep-derived fibrin glue. Six Marino sheep were used to collect concentrate fibrinogen preparation by the ammonium sulfate precipitation method. Crossbred pigs (n=6) were used to evaluate hemostatic efficacy by monitoring whole blood clotting time by the glass slide method, bleeding time and bleeding quantity during liver biopsy. Mean whole blood clotting time on a glass slide started at 201.1±90.47 and 4.43±3.73 seconds, and was completed at 447.83±63.77 and 31.93±4.28 seconds in control and treatment …


Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech Sep 2018

Evaluation Of A Commercial Elisa Test Kit On Classical Swine Fever Antibody Detection Using Oral Fluid Samples, Panchan Sitthicharoenchai, Yonlayong Woonwong, Korakrit Poonsuk, Jirapat Arunorat, Chonnatcha Muangpaisarn, Kanokwan Samatiwat, Worapatch Konthong, Whannaphorn Sattathara, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV) contributes to economic loss of swine production in endemic countries. Serum neutralization and enzyme-linked immunosorbent assay (ELISA) are serological tests commonly used for monitoring CSFV antibody status with serum samples. This experiment evaluated the detection of CSFV antibody in oral fluid samples in commercial ELISA test kit by in vitro study of negative oral fluid mixed with serum of known CSFV serum neutralizing (SN) titer and in vivo oral fluid samples obtained from experimental animals. Correlations of SN titer and S/P ratio of ELISA were observed with the in vitro oral fluid samples, indicating the …


Detection Of Prrsv Circulation Using Oral Fluid Samples For Nursery Management In Endemically Prrsv-Infected Farms, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Chaitawat Sirisereewan, Teerawut Nedumpun, Korakrit Poonsuk, Yaowalak Panyasing, Pariwat Poolperm, Alongkot Boonsoongnern, Roongroje Thanawongnuwech Mar 2018

Detection Of Prrsv Circulation Using Oral Fluid Samples For Nursery Management In Endemically Prrsv-Infected Farms, Yonlayong Woonwong, Roongtham Kedkovid, Jirapat Arunorat, Chaitawat Sirisereewan, Teerawut Nedumpun, Korakrit Poonsuk, Yaowalak Panyasing, Pariwat Poolperm, Alongkot Boonsoongnern, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Porcine reproductive and respiratory syndrome (PRRS) has become a major swine disease worldwide. Relevant management strategies and diagnostic assays are of importance for PRRS virus (PRRSV) control. The objective of this study was to determine the use of oral fluids for PRRSV monitoring in endemically PRRSV-infected herds. PRRSV RNA and PRRSV-specific antibodies were monitored using oral fluid samples and serum samples in two conventional swine farms in Thailand (‘Farm A’, a one-site conventional system farm, and ‘Farm B’, a one-site P0-P1 segregation system farm) during farrowing to nursery periods. Both PRRSV RNA and PRRSV antibodies were detected from 3 to …


Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt Jan 2018

Alkaline Stabilization Of Manure Slurry Inactivates Porcine Epidemic Diarrhea Virus, Erin E. Stevens, Daniel N. Miller, Bethany A. Brittenham, Sarah J. Vitosh-Sillman, Bruce W. Brodersen, Virginia L. Jin, John D. Loy, Amy M. Schmidt

School of Veterinary and Biomedical Sciences: Faculty Publications

Hydrated lime manure treatment was evaluated to determine porcine epidemic diarrhea virus (PEDV) susceptibility to alkaline stabilization. At pH 10, PEDV decreased (quantitative polymerase chain reaction) and lost infectivity (swine bioassay). Although ammonium decreased above pH 9 (up to 25%), alkaline stabilization managed to control potential infection from manure sources.


Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford Jan 2018

Anticoagulant Rodenticide Residues In Game Animals In California, Stella C. Mcmillin, Robert H. Poppenga, Shannon C. Chandler, Deana L. Clifford

USDA Wildlife Services: Staff Publications

Anticoagulant rodenticides (ARs) are used to control rodents around homes, buildings, and in agriculture. They have been found widely in predatory and scavenging wildlife as a result of secondary exposure and less commonly in herbivores and omnivores from primary exposure. While predators and scavengers have been monitored for AR exposure, very little information is available about AR residues in edible muscle tissue of game animals. Game animals may be exposed to ARs through direct consumption of bait, ingestion of contaminated food or vegetation, or consumption of contaminated prey items. Carcasses of three species of game animals (black bear, wild pigs, …


Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang Jan 2017

Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang

Kansas Agricultural Experiment Station Research Reports

Enterotoxigenic Escherichia coli (ETEC) bacteria are the primary cause of diarrheal disease, especially porcine post-weaning diarrhea (PWD). Post-weaning diarrhea is one of the most common diseases in piglets 3 to 10 days after weaning and causes the loss of millions of dollars annually to United States swine industry and other countries. These ETEC bacteria produce two types of virulence factors: 1) fimbriae adhesins, which promote bacterial attachment and colonization in pig small intestine; and 2) enterotoxins that disrupt fluid homeostasis and cause fluid hype-secretion and watery diarrhea. The F4 (K88) is the most important fimbria in ETEC bacteria causing PWD. …


Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz Jan 2016

Evaluating The Impact Of Vevovitall And/Or Crina As Potential Porcine Epidemic Diarrhea Virus Mitigation Strategies As Determined By Polymerase Chain Reaction Analysis And Bioassay, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, M. D. Tokach, J. M. Derouchey, R. D. Goodband, R. A. Cochrane, C. R. Stark, J. Bergstrom, Phillip Charles Gauger, J. Bai, Qi Chen, Jianqiang Zhang, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Feed and feed ingredients have been shown to be potential vectors of porcine epidemic diarrhea virus (PEDV). Potential strategies to mitigate the risk of disease transmission via feed and feed ingredients would be valuable to the swine and feed milling industries. Therefore, the objective of this experiment was to determine the impact of VevoVitall (5,000 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), CRINA (200 ppm; DSM Nutritional Products Inc., Parsipanny, NJ), and a combination of both products (COMBINATION; 5,000 ppm VevoVitall and 200 ppm CRINA) as feed additives with potential to mitigate the risk of PEDV, in swine gestation diet …


Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz Jan 2016

Evaluation Of The Effects Of Flushing Feed Manufacturing Equipment With Chemically- Treated Rice Hulls On Porcine Epidemic Diarrhea Virus Cross Contamination During Feed Manufacturing, J. T. Gebhardt, J. C. Woodworth, C. K. Jones, Phillip Charles Gauger, M. D. Tokach, J. M. Derouchey, R. D. Goodband, M. Muckey, R. A. Cochrane, M. Niederwerder, C. R. Stark, J. Bai, Qi Chen, Jianqiang Zhang, Alejandro Ramirez, Rachel J. Derscheid, Rodger G. Main, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

Various strategies have been proposed to mitigate potential risk of porcine epidemic diarrhea virus (PEDV) transmission via feed and feed ingredients. Wet decontamination has been found to be the most effective decontamination of feed mill surfaces; however, this is not practical on a commercial feed production-scale. Another potential mitigation strategy, easier to implement, would be using chemically-treated rice hulls flushed through the feed manufacturing equipment. The objective of this experiment was to determine the impact of MCFA- or formaldehyde-treated rice hull flush batches as potential PEDV mitigation strategies during feed manufacturing. Feed without evidence of PEDV RNA contamination was inoculated …


Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala Jan 2016

Quality Of Oocytes In Prepubertal And Pubertal Swine, Rita Grabowska, Barbara Blaszczyk, Tomasz Stankiewicz, Tomasz Banas, Sarah Hale, Jan Udala

Turkish Journal of Veterinary & Animal Sciences

In vitro fertilization is a common method of fertilization in the case of both humans and animals. Although this method has developed very rapidly, there are still many unanswered questions about the optimal characteristics that oocytes should have for performing this procedure. The object of this study was to describe the correlation between the diameter and size of swine oocytes in relation to pubescence. The research was conducted on ovaries of piglets (n = 65) and adult swine (n = 69). Each oocyte was examined with and without the zona pellucida. The analysis showed that the average size of the …


Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones Jan 2016

Evaluating The Inclusion Level Of Medium Chain Fatty Acids To Reduce The Risk Of Porcine Epidemic Diarrhea Virus In Complete Feed And Spray-Dried Animal Plasma, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Drew Robert Magstadt, Paulo Elias Arruda, Alejandro Ramirez, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of MCFA levels below 2% inclusion is unknown. The objective of this experiment was to evaluate if a 1% inclusion of MCFA is as effective at PEDV mitigation as a 2% inclusion or formaldehyde in swine feed and spray-dried animal plasma (SDAP). Treatments were arranged in a 4 × 2 × 7 plus 2 factorial with 4 chemical treatments: 1) PEDV positive with no chemical …


Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones Jan 2016

Assessing The Effects Of Medium Chain Fatty Acids And Fat Sources On Porcine Epidemic Diarrhea Virus Viral Rna Stability And Infectivity, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, M. Saensukjaroenphon, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rachel J. Derscheid, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Research has confirmed that chemical treatments, such as medium chain fatty acids (MCFA) and commercial formaldehyde, can be effective to reduce the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination in feed. However, the efficacy of individual MCFA levels are unknown. The objective of this study is to compare the efficacy of commercially-available sources of MCFA and other fat sources versus a synthetic custom blend of MCFA to minimize the risk of PEDV cross-contamination as measured by qRT-PCR and bioassay. Treatments were arranged in a 17 × 4 plus 1 factorial with 17 chemical treatments: 1) Positive control with PEDV …


Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand, David C. Love, Prasit Tharavichitkul, Orapun Arjkumpa, Maho Imanishi, Soawapak Hinjoy, Kenrad Nelson, Keeve E. Nachman Mar 2015

Antimicrobial Use And Multidrug-Resistant Salmonella Spp., Escherichia Coli, And Enterococcus Faecalis In Swine From Northern Thailand, David C. Love, Prasit Tharavichitkul, Orapun Arjkumpa, Maho Imanishi, Soawapak Hinjoy, Kenrad Nelson, Keeve E. Nachman

The Thai Journal of Veterinary Medicine

The aim of this study was to isolate antimicrobial-resistant bacteria from intensive commercial and backyard swine operations (farms) in Northern Thailand and to characterize antimicrobial usage among swine farmers. A total of 51 swine farms in Chiang Mai, Lampoon, and Nan provinces were visited from February to October 2011 to collect swine fecal samples for bacterial isolation (Salmonella spp., Escherichia coli, Enterococcus faecalis) and antimicrobial resistance testing. A survey about production intensity, management practices, animal health, and antimicrobial usage was conducted with a sub-set of farms (n = 20) located in Nan province. The farm-level prevalence of drug-resistant E. coli, …


Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones Jan 2015

Effect Of Thermal Mitigation On Porcine Epidemic Diarrhea Virus (Pedv)- Contaminated Feed, R. A. Cochrane, L. L. Schumacher, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, Qi Chen, Jianqiang Zhang, Phillip Charles Gauger, Rodger G. Main, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. However, epidemiological evidence has shown that swine feed and ingredients may serve as potential vectors of transmission. Since it is known that PEDV is a heat-sensitive virus, we hypothesized that a conditioner and pellet mill mimicking commercial thermal processing would mitigate PEDV infectivity. To test this hypothesis, two experiments were designed to determine if different pellet mill conditioner retention times or temperatures would impact PEDV infectivity determined by polymerase chain reaction (PCR) analysis and bioassay. For the first study, a 3×3×2 factorial was utilized, with three pelleting temperatures (155, …


Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones Jan 2015

Evaluating Chemical Mitigation Of Porcine Epidemic Diarrhea Virus (Pedv) In Swine Feed And Ingredients, R. A. Cochrane, S. S. Dritz, J. C. Woodworth, A. R. Huss, C. R. Stark, R. A. Hesse, Jianqiang Zhang, M. D. Tokach, J. Bai, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

Porcine Epidemic Diarrhea Virus (PEDV) is primarily transmitted by fecal-oral contamination. Research has confirmed swine feed or ingredients as potential vectors of transmission, so strategies are needed to mitigate PEDV in feed. The objective of this experiment was to evaluate the effectiveness of various chemical additives to prevent or mitigate post-processing PEDV contamination in swine feed and ingredients. Treatments were arranged in a 7 × 4 factorial with seven chemical treatments and four feed matrices. The chemical treatments included: negative control with no chemical addition, 0.3% commercial formaldehyde product, 1% sodium bisulfate, 1% sodium chlorate, 3% custom organic acid blend …


Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach Jan 2015

Utilizing Feed Sequencing To Decrease The Risk Of Porcine Epidemic Diarrhea Virus (Pedv) Cross-Contamination During Feed Manufacturing, L. L. Schumacher, R. A. Cochrane, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach

Kansas Agricultural Experiment Station Research Reports

Understanding key points of potential cross-contamination during the feed manufacturing process is important to developing efficacious methods to control or prevent transmission of pathogens into swine diets. In this study, an experiment was conducted involving 30 crossbred 10-d-old pigs that were used as a bioassay model for Porcine Epidemic Diarrhea Virus (PEDV) to determine the effects of feed batch sequencing on PEDV cross-contamination and subsequent infectivity. PEDV with a PCR cycle threshold value (Ct) of 11 was uniformly mixed into 4.5 kg of swine diet using a stainless steel bench top mixer validated for mixing efficiency. The inoculated feed was …


Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao Jan 2015

Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao

Turkish Journal of Veterinary & Animal Sciences

PCR-RFLP analysis of intron 17 of the MUC4 gene was conducted in Large White pigs and associations with cytokine levels (IFN-?, IL-1ß, IL-4, IL-6, IL-8, IL-10, TGF-ß, and TNF-?), production traits (100-kg-weight day age, back-fat depth, and eye muscle depth), and reproductive performance within different litters (total number born, number born alive, number in live weaning litter) were analyzed with the aim of identifying genetic markers of disease resistance. The results revealed AA, AG, and GG genotypes, with an A243G mutation identified in the GG genotype. Correlation analysis indicated that IL-8 and IL-10 levels and 100-kg-weight day age were significantly …


Assessment Of Swine (Sus Scrofa Domesticus) Alveolar Macrophage Viability Associated With Heavy Metal Air Pollutants, Gemerlyn G. Garcia, Eduardo H. Grivialde, Lerma C. Ocampo, Claro N. Mingala Dec 2014

Assessment Of Swine (Sus Scrofa Domesticus) Alveolar Macrophage Viability Associated With Heavy Metal Air Pollutants, Gemerlyn G. Garcia, Eduardo H. Grivialde, Lerma C. Ocampo, Claro N. Mingala

The Thai Journal of Veterinary Medicine

The impact of air pollution on airway cellular defense of pigs raised under backyard, semi-commercial and commercial conditions was investigated. Flame atomic absorption spectrometry was used to analyze Cadmium (Cd) and Lead (Pb) while manual cold vapor atomic absorption spectrometry was applied to evaluate Mercury (Hg) contents of dust particles from farms. Tests for viable counts of swine alveolar macrophages (SAM) that simulate animal responses to air pollutants, microbiological techniques for the recovery of bacterial cells and evaluation for cellular function were undertaken. Results showed that air pollution was accompanied by significant high levels of Pb and Cd. Exposure of …


Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen Jul 2012

Plasmid Profiles Of Multidrug-Resistant Escherichia Coli From Clinically Healthy Swine, Khin Khin Lay, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 145 Escherichia coli isolates originated from clinically-healthy pigs were analyzed for plasmidprofiles. All but one isolate (n=144) carried one to nine plasmids with size ranging from 0.7-16.2 Mda. Fifty-sixplasmid profiles were defined based on sizes and numbers of plasmids in each strain, of which the most commonpatterns were 15.8, 4, 3.3, 1.7, 1.3 Mda and 16.2, 15.8, 4, 3.3, 2.3, 1.7, 1.3, 1, 0.7 Mda. The results indicate widedistribution of plasmids among the commensal E. coli isolates originated from clinically-healthy pigs.


Serotype, Antimicrobial Susceptibility, And Genotype Of Salmonella Isolates From Swine And Pork In Sa Kaew Province, Thailand, Chaiwat Pulsrikarn, Phattharaphron Chaichana, Srirat Pornruangwong, Yukio Morita, Shigeki Yamamoto, Sumalee Boonmar Mar 2012

Serotype, Antimicrobial Susceptibility, And Genotype Of Salmonella Isolates From Swine And Pork In Sa Kaew Province, Thailand, Chaiwat Pulsrikarn, Phattharaphron Chaichana, Srirat Pornruangwong, Yukio Morita, Shigeki Yamamoto, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

In January 2010, we examined the prevalence of Salmonella in the rectal swab of 66 swine on 8 farms and in 25 pork products at 6 meat retailers of Sa Kaew province. Salmonella was isolated from 3%(2/66) of swine rectal swab samples and 96%(24/25) of pork samples. In rectal swab samples, both S. Weltevreden and S. Dumfries were isolated from one sample, and S. Stanley from another sample. In pork, a total of 42 isolates (16 serovars) were found ; 8 strains of S. Rissen, 5 strains of S. Stanley and S. Anatum, 4 strains of S. Give and S. …


Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang Jan 2012

Prevalence And Patterns Of Antimicrobial Resistant Of Fecal Escherichia Coli Isolates Among Pigs On Eight Farrow-To-Finish Farms And Their Production Environments In Shandong Province, China, Hongchao Zhou, Lin Liu, Zhaofang Tian, Wenjuan Zhang

Turkish Journal of Veterinary & Animal Sciences

The main objectives of this study were to determine the prevalence and antimicrobial resistance patterns of Escherichia coli isolates in feces of pigs and their production environments on farms. A total of 560 isolates of Escherichia coli, from 112 samples obtained from weaner and finisher pigs, feed, water, and house sparrows on 8 farrow-to-finish farms, were tested for susceptibility to 16 antimicrobials. The prevalence of resistance varied widely (0.0% to 69.5%) among the antimicrobials tested. From the tested swines, 75.75% of the isolates were resistant to one or more antimicrobials. Resistance was significantly more frequent (P = 0.005, P < 0.01) in swine isolates compared to those from feed, water, and sparrows and significantly more frequent (P = 0.007, P < 0.01) in weaner pigs compared to finisher pigs. The findings indicate that resistance to a broad range of antimicrobials was prevalent among fecal E. coli isolates of swine on the study farms. Fortunately, E. coli was not isolated from the feed, water, and house sparrows.


Salbutamol Residues In Swine Tissues And Body Fluids After Feeding, Jen Hung Ming, Hsiang Huang Han, Ling Chen Chao, Jen Wu Yu, Katie Marie Dixon, Liang Mao Chi Dec 2010

Salbutamol Residues In Swine Tissues And Body Fluids After Feeding, Jen Hung Ming, Hsiang Huang Han, Ling Chen Chao, Jen Wu Yu, Katie Marie Dixon, Liang Mao Chi

The Thai Journal of Veterinary Medicine

Salbutamol residues in swine tissues and fluids were investigated. Tissue, serum and urine samples were collected from 33 pigs after 14 days of oral administration of 3 ppm salbutamol. The concentrations of salbutamol were determined by enzyme-linked immunosorbent assay and further confirmed by capillary electrophoresis. The highest urinary concentration of salbutamol was 145.12 ng/ml at 18 hour and remained detectable for 30 days after the last feeding. Salbutamol was undetectable in serum samples. The highest concentrations of salbutamol in liver, kidney, lung, heart, brain and muscle were 70.42 ng/g, 31.88 ng/g, 26.06 ng/g, 6.76 ng/g, 3.41 ng/g and 2.97 ng/g, …