Medicine and Health Sciences Commons^™

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …

Kinetics Of The Chromosome 14 Microrna Cluster Ortholog And Its Potential Role During Placental Development In The Pregnant Mare, Pouya Dini, Peter Daels, Shavahn C. Loux, Alejandro Esteller-Vico, Mariano Carossino, Kirsten E. Scoggin, Barry A. Ball

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The human chromosome 14 microRNA cluster (C14MC) is a conserved microRNA (miRNA) cluster across eutherian mammals, reported to play an important role in placental development. However, the expression kinetics and function of this cluster in the mammalian placenta are poorly understood. Here, we evaluated the expression kinetics of the equine C24MC, ortholog to the human C14MC, in the chorioallantoic membrane during the course of gestation.

Results: We demonstrated that C24MC-associated miRNAs presented a higher expression level during early stages of pregnancy, followed by a decline later in gestation. Evaluation of one member of C24MC (miR-409-3p) by in situ hybridization …

Access To Veterinary Care: Barriers, Current Practices, And Public Policy, Access To Veterinary Care Coalition

Faculty Publications and Other Works -- Small Animal Clinical Sciences

Pets have become an integral part of our families with over 80% of pet owners reporting that they consider their pets to be a member of their family. An estimated 29 million dogs and cats live in families that participate in the Supplemental Nutrition Assistance Program. There are also middle-class families that live paycheck to paycheck, with limited funds for veterinary care. These families deserve the companionship of pets to enjoy the mental, physical, and emotional benefits that come from this human-animal bond.

Through a grant from Maddie’s Fund®, the Access to Veterinary Care Coalition (AVCC) commissioned a national …

Hitting The Threshold Of Common Sense: The Time For Screening Limits To Guard Against Environmental Transfer Is Now, Clara Fenger, Thomas Tobin, Maria Catignani, Theodore Shults

Maxwell H. Gluck Equine Research Center Faculty Publications

Recreational drug use and the opiate epidemic have taken a great toll on the human population of the United States and beyond. Year over year, deaths from synthetic opioids—of which fentanyl ranks as the most prevalent—have skyrocketed, reaching 29,406 in 2017. Environmental transfer of drugs of human addiction to horses is nothing new. Cocaine and its primary metabolite, benzoylecgonine, have long been identified as environmental substances in post-race samples, and many jurisdictions have screening limits in place as a result. More recently, methamphetamine has begun to show up in post-race samples, reflecting the increasing addiction problem associated with this drug. …

Comparative Ultrasonographic And Computed Tomographic Images Of The Adrenal Glands Of Healthy Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

Feline adrenal disorders that affect the size of the adrenal glands can be detected by diagnostic imaging including ultrasonography and Computed Tomography (CT). The objectives of this study were to compare the appearance of the adrenal gland in healthy cats between these two techniques and establish the reference values of the CT dimensions of the adrenal gland in cats. We found that there was no significant difference in the length of both adrenal glands between the two techniques but the cranial and caudal heights of the left adrenal glands and cranial height of the right adrenal gland obtained from the …

Surgical Treatment For Tubular Colonic Duplication Communicating To Retroperitoneal Space In An Adult Dog, Nithida Boonwittaya, Piyathip Choochalermporn, Sunee Kunakornsawat, Teerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Canine colonic duplication is a rare congenital anomaly, usually symptomatic in the first year of life, with the common signs of bowel obstruction. Most importantly, colonic duplication in an adult dog has not been reported. This case document reports a non-communicating tubular colonic duplication with concurrent retroperitoneal mass in a 3- year-old female crossbred dog, presented for further diagnosis and treatment of the abdominal mass. Without any remarkable clinical signs, physical examination noted only abdominal distension with mild discomfort. Ultrasonography demonstrated a large mass in the mid-caudal abdomen, a tubular mass appearance on the colonic wall and the presence of …

Quantitative Microbial Risk Assessment For Parts, Ground, And Msc Poultry Product Including Intervention Analysis And Exploration Of Enterobacteriaceae As An Indicator Organism In Poultry Processing, Leigh Ann Parette

Graduate Theses and Dissertations

Samples collected at five different large bird poultry processing facilities over a period of 7 months from prescald to post debone locations were enumerated for Enterobacteriaceae, Salmonella spp., and Campylobacter spp. and the results were used to create Quantitative Microbial Risk Analyses (QMRA) models for parts, ground, and mechanically separated chicken (MSC) products. Sensitivity analyses indicated the points in the process at which reductions would be most advantageous to the endpoint and simulation models were run to test reductions required to meet the current USDA performance standards.

These data were analyzed to determine the reductions from one node (location) to …

A Preliminary Study On Diversity Of Midgut Microbiota In Aedes Aegypti (Linnaeus) And Culex Quinquefasciatus (Say) Collected From Bangkok, Thailand, Sonthaya Tiawsirisup, Jirinnut Sirijutalak, Mananya Sondang, Manunya Supol, Nut Ansusinha, Ranida Tuanudom, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Aedes aegypti and Culex quinquefasciatus are important mosquito vectors for many infectious diseases. A number of factors affect the vector competence of these mosquitoes for a specific pathogen. The bacteria harbored in the midgut are known to influence mosquito physiology and can alter the response to various pathogens. Bacteria from the midgut of Ae. aegypti and Cx. quinquefasciatus were cultured and identified using bacteriological and molecular techniques in this study in which two groups of mosquitoes were examined. The first group was laboratory- reared, and the second group were field- collected mosquitoes from Bangkok. Twelve bacterial genera ( i. e. …

Plasmid Profile Of Enterococcus Faecium And Enterococcus Faecalis Isolated From Pigs, Pork And Humans In Thai-Laos Border Provinces, Wink Phyo Thu, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

This study aimed to determine plasmid profile of E. faecium and E. faecalis isolated from pigs, pig products and humans in Thailand-Laos border provinces. A total of 96 Enterococcus spp. including E. faecium (n=59) and E. faecalis (n=37) from pigs, pork and humans were included. All were determined for their antimicrobial susceptibilities and plasmid profile. Most isolates (96.9%) carried one to four plasmids with a molecular weight of 0.03-35 kb in E. faecium and 19-40 kb in E. faecalis. The 26 kb plasmid was most commonly detected. Variable plasmid profiles were defined, of which the most common pattern was, 23 …

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram

The Thai Journal of Veterinary Medicine

A 10-year-old female Pomeranian dog was presented to the Small Animal Teaching Hospital, Chulalongkorn University, on December, 2018, with a history of anorexia, frequent vomiting, depress and oliguria. Physical examination revealed that the dog was depressed but responsive to the stimuli. The dog was dehydrated (7%) with pale-pink mucous membrane. The capillary refilling time of 1.5 seconds with strong femoral pulse were detected. The respiratory rate was 65 breath per minute with normal sound while heart rate was approximately 60 beats per minute without cardiac murmur from auscultation using stethoscope. The systolic blood pressure was 120 mmHg. The blood collection …

Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye

Graduate Theses and Dissertations

Campylobacter jejuni is a prevalent infectious enteritis mainly foodborne from chickens. Despite of reducing C. jejuni food contamination dramatically decreases campylobacteriosis, few effective approaches are available for the bacterial reduction in chickens. The aim of this study was to use microbial metabolic product deoxycholic acid (DCA) to reduce C. jejuni chicken colonization. Broiler chicks were fed 0 or 1.5 g/kg DCA, lithocholic acid (LCA), or urodeoxycholic acid (UDCA) in diets or orally gavaged with cholic acid (CA, 1.5g/kg body weight). Birds were also transplanted with DCA modulated anaerobes (DCA-Anearo) or aerobes (DCA-Aero). Birds were infected with 109 CFU/bird human clinical …

Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land

Master's Theses

As keeping backyard poultry rises, human contact with zoonotic pathogens will increase. One such pathogen that backyard enthusiasts have exposure risks to is Salmonella spp. which may cause a potential public health threat due to its increasing multidrug resistancy. Salmonella spp. were present in 33 of 50 samples collected from 29 sites with backyard poultry coops in San Luis Obispo County during March to May in 2014. Two different Hardy-CHROME™ Salmonella Selective Media plates were used to culture and isolate positive samples of Salmonella spp.. Each positive isolate was tested for antimicrobial sensitivity to 6 standard antibiotics: Ampicillin, Bacitracin, …

Oxidative Stress And Antioxidant In Canine Cutaneous Mast Cell Tumors, Arayaporn Macotpet, Ekkachai Pattarapanwichien, Fanan Suksawat, Patcharee Boonsiri

The Thai Journal of Veterinary Medicine

Oxidative stress can result from either the excessive production of reactive oxygen species (ROS) or an impaired antioxidant system, or both. It causes damage to lipids, proteins and DNA. Therefore, oxidative stress may be involved in carcinogenesis, and is associated with many types of cancer in dogs. The objective of this study was to compare the levels of malondialdehyde, protein hydroperoxides, glutathione, retinol and alpha-tocopherol between dogs with mast cell tumors and clinically healthy controls. Blood samples were obtained from eighteen clinically healthy dogs and fourteen dogs with spontaneous mast cell tumors. Malondialdehyde and protein hydroperoxides levels were measured by …

Impact Of Feed Characteristics For Monitoring Afb1 Contamination In Commercial Concentrate Feeds Used In Small Holder Dairy Farms, Chiangmai, Thailand, Wasana Chaisri, Wantanwa Mongkon, Yoshigo Sugita-Konishi, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the impact of abnormal physical characteristics for monitoring aflatoxin B1 (AFB1) contamination in commercial dairy concentrate feeds. The study was conducted during March to May 2013 using 82 dairy farms in Chiang Mai province, Thailand. All feed samples were collected from new packages and their physical characteristics were subsequently determined. AFB1 concentrations in the samples were measured using the enzyme-linked immunosorbent assay (ELISA), and feed samples with AFB1 of more than 5 µg/Kg were determined as AFB1 contaminated samples. The impact of physical characteristics on AFB1 contamination were evaluated using a multiple …

Review Of The Cardiovascular Toxicity Of Amitriptyline Treatment For Canine Neuropathic Pain, Saikaew Sutayatram, Kumpanart Soontornvipart, Piyasiri Glangosol

The Thai Journal of Veterinary Medicine

Nowadays, the lifespan of pets has been increased by improvements in veterinary practice but age-related disorders are also increasing. Several nervous system attenuations are well-known to be the result of aging and/or pathological lesions. In many cases, neuropathic pain (NP) can develop and cause a significant impact on the clinical outcome and quality of life. With the difficulty in identification of the specific cause of NP, most NP management is planned according to clinical manifestations and recommended trial therapy. The drug of choice for trial therapy seems to be amitriptyline, a tricyclic antidepressant with multiple effects on neurotransmitter receptors and …

Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study determined the efficacy of recombinant HVT- NDV ( rHVT- NDV) vaccine simultaneously vaccinated with live vaccine at 1 day old and a booster vaccination with live vaccine at 10 days old against Asiatic, Genotype VII NDV in broiler chickens. The chickens were divided into 5 groups. The chickens in groups 1, 2 and 3 were vaccinated with rHVT- NDV vaccine and different live vaccines at 1 day old, and then finally received a booster vaccination at 10 days old. The chickens in groups 4 and 5 served as the positive and negative control groups, respectively. The chickens in …

High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

Zika virus (ZIKV), chikungunya virus (CHIKV) and dengue virus (DENV) are emerging and re-emerging arboviral diseases. These viruses are transmitted to humans through the bites of Aedes mosquitoes. Recently, ZIKV infection has been described as an emerging disease in Thailand and many countries, especially in tropical and subtropical areas. Specific drugs and vaccines against these infections are unavailable; therefore, effective disease control relies on vector control measures only. To understand the transmission cycle of these viruses and mosquito vectors, this study is designed to investigate the natural infection of ZIKV, CHIKV and DENV in field-caught mosquitoes by molecular techniques. Adults …

Emergence Of A Classical Variant Of Porcine Epidemic Diarrhea Virus Novel To Thailand Responsible For The Milder Clinical Disease In A Herd Previously Infected With A Pandemic Variant, Gun Temeeyasen, Anchalee Srijangwad, Thitima Tripipat, Angkana Tantituvanont, Dachrit Nilubol

The Thai Journal of Veterinary Medicine

In 2014, porcine epidemic diarrhea (PED) outbreaks characterized by mild clinical disease and low preweaning mortality increased in frequency in Thailand. An outbreak investigation, based on the detection of the classical variant and the allowance of the herd owners, was conducted in 5 swine herds and 25 intestinal samples were submitted from herds with outbreaks of diarrhea for sequencing analysis. PEDV isolation and complete nucleotide sequences of the spike (S) glycoprotein were determined. The PEDV isolate EAS1 was identified. Phylogenetic analysis based on the full-length spike gene demonstrated that EAS1 belonged to the classical variant group with high nucleotide and …

Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy

The Thai Journal of Veterinary Medicine

Kaempferol, myricetin and quercetin are abundant flavonols in edible fruit and vegetables. Much previous data has shown the beneficial effects of these flavonols in cancer treatments. Thus, the cytotoxic effects on rhesus monkey kidney epithelial cells (LLC-MK2) and the anti-proliferative effects on human cervical cancer (HeLa) cells through total ERK protein expression of kaempferol, myricetin and quercetin have been investigated. The cytotoxic assay at 24h revealed the high safety of three flavonols on LLC-MK2 cells. Kaempferol (1-1000 µM) did not have any significant effect on the viability of these normal epithelial cells. The cytotoxicity of myricetin and quercetin was 5 …

Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang

The Thai Journal of Veterinary Medicine

The frozen goat semen process must wash seminal fluid from goat semen. A small dosage of alpha1 adrenoceptor antagonist or tamsulosin may reduce seminal fluid in goat. A latin square 4x4 was applied to twelve male goats to receive either normal saline (NSS), tamsulosin 3, 6 and 9 µg/kg intramuscular injection at one-week intervals. Semen collection by artificial vagina and libido scoring was performed within one-hour post injection. Semen quality and seminal fluid were evaluated after semen collection The results showed that none of the libido scores in the groups was different. Anejaculations occurred in 1 (8.3%), 3 (25%) and …

Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The potential value of bolus tracking contrast enhanced computed tomography (CT) in the diagnosis of oral tumors in dogs was investigated by examining the relationship between contrast-enhanced CT image density and microvascularization. Among 20 dogs with oral tumors, aged 8 – 17 years, weight 3.6 – 40.0 kg, 13 dogs were suffering with melanoma (MM), 4 with squamous cell carcinoma (SCC), and 3 with fibrosarcoma. Based on contrast-enhanced CT scan, the time lag from the injection of contrast medium into the right cephalic vein until the contrast medium returned to the mid-cervical external jugular vein at the level of the …

Comparison Of Sperm Freezability And Fertility Among Hmong Black-Bone, Barred Plymouth Rock And Thai Native (Pradu Hang Dam) Chickens, Saennamphung Somtaw, Pornjit Sonseeda, Nalinee Tubtimtong, Yupin Phasuk, Thevin Vongpralub

The Thai Journal of Veterinary Medicine

Semen cryopreservation is the most useful method for the long-term storage of chicken genetic resources. The objective of this study was to compare post- thaw semen quality of Hmong Black- Bone to Barred Plymouth Rock and Thai native ( Pradu Hang Dam) chickens in terms of sperm motility, viability and fertility. A total of 9 males of each breed were used; semen collected from each cock was subsequently evaluated under a microscope. Semen samples were diluted with Schramm diluent, cooled down from 20– 25oC to 5oC and diluent plus DMF ( dimethylformamide) was added (6% DMF of final volume when …

Comparative Effect Of Dl-Methionine And Dl-Methionine Hydroxy Analogue Supplemented Diet On Productive Performance, Fat Accumulation And Lipid Profile In Blood Of Meat-Type Ducks, Chanwit Kaewtapee, Chaiyapoom Bunchasak

The Thai Journal of Veterinary Medicine

The objective of this study was to compare the effect of DL-methionine (DLM) and DL-methionine hydroxy analogue (DL-MHA) supplementation on the growth performance, carcass quality, fat accumulation and lipid profile in blood of meat-type duck. Three hundred male Cherry Valley ducks were divided into 3 groups with 4 replications of 25 ducklings each. Each group was given a diet as follows: 1) basal diet, 2) basal diet + DLM, and 3) basal diet + DLMHA. The results show that dietary supplementation with DLM and DL-MHA increased body weight (P<0.01), feed intake (P<0.01) and pectoralis major (P<0.05), but decreased abdominal fat (P<0.01) when compared to the basal diet. Supplementation with DL-MHA also resulted in greater levels of triglycerides (P<0.05), low-density lipoprotein (P<0.05) and cholesterol (P<0.05) in the blood compared to the DLM group, in addition to greater levels of triglycerides (P<0.05) and high-density lipoprotein (P<0.05) compared to the basal diet. Furthermore, supplementation with DLM was shown to lower the level of plasma uric acid (P<0.05) compared to the basal diet. It is concluded that DL-MHA can promote the same productive performance in meat-type ducks as DLM, but the fat accumulation and lipid profile in blood was greater with the DL-MHA supplementation than with DLM. Furthermore, the underlying mechanism of different effects between DL-MHA and DLM on the lipid metabolism in ducks should be further investigated.

Survival Time And Prognosis Factors In Hypertrophic Cardiomyopathy Cats With Congestive Heart Failure, Lalida Tantisuwat, Panduangjai Puangampai, Pornchanok Panpakdee, Thitinan Tangarsasilp, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Hypertrophic cardiomyopathy (HCM) is a common myocardial disease in cats. Nowadays, there is no study determining the survival time of cats affected with HCM in Thailand. This study aimed to evaluate the survival time in HCM cats with congestive heart failure (CHF) in Thailand and to determine the factors associating with survival time. A retrospective study was performed in 47 HCM cats to analyze survival time and the association of survival time and factors including sex, age, breeds, weight, physical findings, the presence of complications, medication and echocardiographic findings. The data was analyzed by the Log-Rank test and displayed with …

Effects Of Supplementing Varying Levels Of 1,3-Diacylglycerol (Dag) On Growth Performance, Apparent Nutrient Digestibility And Selected Fecal Microbial Populations In Growing Pigs, Wen Chao Liu, Kwan Sik Yun, In Ho Kim

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate the effects of 1,3-diacylglycerol (DAG) supplementation on growth performance, apparent digestibility and selected fecal microbial populations in growing pigs. A total of 80 crossbred pigs [(Landrace × Yorkshire) × Duroc] with an average initial body weight (BW) of 27.52 ± 0.20 kg were used in a 6 week feeding trial. Pigs were allotted to 4 dietary treatments based on their initial BW and sex (5 replications; 4 pigs per pen with 2 barrows and 2 gilts). Dietary treatments included: 1) CON, basal diets; 2) CON+0.075% 1,3-DAG; 3) CON+0.10% 1,3-DAG; 4) CON+0.15% …

Food Is Medicine, Katherine Keane

Senior Honors Projects

No abstract provided.

Veterinary Telemedicine Perception And Utilization Intention, Chandler Hawk

Chancellor’s Honors Program Projects

No abstract provided.

Comparison Of Isolation Techniques For Bone Marrow Derived Canine Mesenchymal Stem Cells (Mscs) And The Compatibility Of Mscs Loaded Onto Polycarpolactone Hydroxyapatite, Chalika Wangdee, Sasithorn Panasophonkul, Napaphat Thadavirul, Kumpanart Soontornvipart, Prasit Pavasant, Pitt Supaphol, Mongkol Techakumphu, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Bone marrow was collected from 7 dogs and submitted to 3 different MSC isolation techniques (direct plating, red blood cell lysis and gradient density). The number of cells, expression of MSC markers and in vitro the osteogenic differentiation obtained from each technique were examined. In order to study in vivo the osteogenic capability of derived MSCs, non-union ulna lesions (n=3) were firstly induced by transplantation of a composite of polycarprolactone/ hydroxyapatite (PCL/HAp) scaffold experimentally on to a critical-size ulna bone defect. The MSCs were injected into the lesions. The non-union sites were examined by radiography, angiography and histology at 2, …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

A 6-month-old French Bulldog had been presented with left eye blepharospasm. The puppy had been treated with topical antibiotics for weeks from the local veterinarian. However, blepharospasm still persisted. Ophthalmic examination from distance showed mild conjunctival hyperemia. Excessive tear was observed around the eyeball. Close ophthalmic examinations revealed prominent conjunctival blood vessels at the outer portion of the nictitating membrane. Clump of abnormal hairs were identified at the ventro-nasal region of the palpebral conjunctiva, next to eyelid margin. Schirmer tear test 1 level of the left eye was 25 mm wetness, while it was 16 on the right.

What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A thirteen kilograms, 6-year-old, male, Shih Tzu dog.