Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Natural Products (9)
- Cellular Biology (7)
- Natural Anti Cancer drugs (7)
- Apoptois (6)
- Cancer (6)
-
- Natural product (6)
- Pharmacology (6)
- Syam mohan (6)
- Apoptosis (3)
- Design (2)
- Development (2)
- Engineering (2)
- Packaging (2)
- Packaging Development & Engineering: Business Strategy (2)
- Profit (2)
- Signalling Pathways (2)
- Administrative Law (1)
- Aglaia exima; Meliaceae; Cycloartane; Cytotoxicity; Steroids; 24(E)-cycloart-24-ene-26-ol-3-one (1)
- Alkaloids (1)
- Alkaloids_ Apoptosis (1)
- And Hormone Antagonists Inorganic Chemicals Lipids Macromolecular Substances Nucleic Acids (1)
- And Nucleosides Organic Chemicals Other Chemicals and Drugs Pharmaceutical Preparations Polycyclic Compounds Communication Sciences and Disorders Speech Pathology and Audiology Speech and Hearing Science Dentistry Dental Materials Dental Public Health and Education Endodontics and Endodontology Oral Biology and Oral Pathology Oral and Maxillofacial Surgery Orthodontics and Orthodontology Other Dentistry Pediatric Dentistry and Pedodontics Periodontics and Periodontology Prosthodontics and Prosthodontology Dietetics and Clinical Nutrition Diseases Health and Medical Administration Knowledge Translation Medical Education Medical Sciences Medical Specialties Mental and Social Health Nursing Optometry Organisms Other Medicine and Health Sciences Pharmacy and Pharmaceutical Sciences Medicinal and Pharmaceutical Chemistry Natural Products Chemistry and Pharmacognosy Other Pharmacy and Pharmaceutical Sciences Pharmaceutics and Drug Design Pharmacoeconomics and Pharmaceutical Economics Pharmacy Administration (1)
- And Proteins Biological Factors Biomedical and Dental Materials Carbohydrates Chemical Actions and Uses Complex Mixtures Enzymes and Coenzymes Heterocyclic Compounds Hormones (1)
- And Public Health Veterinary Toxicology and Pharmacology (1)
- Anti-apoptotic proteins (1)
- Antiepileptic drug (1)
- Antipsychotic (1)
- Audiogenic seizures (1)
- Bcl-2 (1)
- Bcl-xL (1)
- Publication Year
Articles 1 - 30 of 32
Full-Text Articles in Medicine and Health Sciences
The Medicine Shoppe V. Loretta Lynch: Pharmacists’ Corresponding Responsibility With Physicians Under Dea Interpretation Of The “Legitimate Medical Purpose” Standard, Jeffrey C. Grass Jd, Ms, Aclm
The Medicine Shoppe V. Loretta Lynch: Pharmacists’ Corresponding Responsibility With Physicians Under Dea Interpretation Of The “Legitimate Medical Purpose” Standard, Jeffrey C. Grass Jd, Ms, Aclm
Jeffrey C. Grass JD, MS, ACLM
The Medicine Shoppe v. Loretta Lynch et al. 14-1223 will assist pharmacists and pharmacy owners in understanding their duties “corresponding responsibility to assure that its prescriptions for controlled substances are issued for a legitimate medical purpose” and “in the practitioner's usual course of professional practice.” In the meantime, pharmacists need to know that they are not immune from administrative, regulatory or criminal prosecution under the CSA solely due to their status. Rather, they are expected to dispense drugs for the bona fide treatment of a patient's disease. Under this regime, he or she must exercise sound professional judgment when evaluating …
Risperidone And Its Deconstructed Analogs: Functional Effects On The 5ht2ar, Sneha Shah
Risperidone And Its Deconstructed Analogs: Functional Effects On The 5ht2ar, Sneha Shah
Sneha Shah
G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that sense extracellular signal and activate intracellular signaling pathways. The serotonin 5HT2A receptor (or 2AR) is one of the GPCRs coupled to Gq proteins, activating PLC and hydrolyzing PIP2. This hydrolysis causes a diffusion of bound PIP2 away from the channel binding site resulting in G protein-gated inwardly rectifying K+ channel (GIRK) inhibition and a downstream stimulation of Ca2+ release from endoplasmic reticulum stores. Previous experiments have demonstrated that the serotonin 5HTA receptor is a target of serotonergic psychedelic drugs, such as LSD, and partially mediates the action of many atypical antipsychotic …
Antiplasmodial And Antioxidant Isoquinoline Alkaloids From Dehaasia Longipedicellata, Kok Hoong Leong
Antiplasmodial And Antioxidant Isoquinoline Alkaloids From Dehaasia Longipedicellata, Kok Hoong Leong
Kok Hoong Leong
The crude extract of the bark of Dehaasia longipedicellata exhibited antiplasmodial activity against the growth of Plasmodium falciparum K1 isolate (resistant strain). Phytochemical studies of the extract led to the isolation of six alkaloids: two morphinandienones, (+)-sebiferine (1) and (−)-milonine (2); two aporphines, (−)-boldine (3) and (−)-norboldine (4); one benzlyisoquinoline, (−)-reticuline (5); and one bisbenzylisoquinoline, (−)-O-O-dimethylgrisabine (6). Their structures were determined on the basis of 1D and 2D NMR, IR, UV, and LCMS spectroscopic techniques and upon comparison with literature values. Antiplasmodial activity was determined for all of the isolated compounds. They showed potent to moderate activity with IC50 values …
Subditine, A New Monoterpenoid Indole Alkaloid From Bark Of Nauclea Subdita (Korth.) Steud. Induces Apoptosis In Human Prostate Cancer Cells, Kok Hoong Leong
Subditine, A New Monoterpenoid Indole Alkaloid From Bark Of Nauclea Subdita (Korth.) Steud. Induces Apoptosis In Human Prostate Cancer Cells, Kok Hoong Leong
Kok Hoong Leong
In this study, a new apoptotic monoterpenoid indole alkaloid, subditine (1), and four known compounds were isolated from the bark of Nauclea subdita. Complete 1H- and 13C- NMR data of the new compound were reported. The structures of isolated compounds were elucidated with various spectroscopic methods such as 1D- and 2D- NMR, IR, UV and LCMS. All five compounds were screened for cytotoxic activities on LNCaP and PC-3 human prostate cancer cell-lines. Among the five compounds, the new alkaloid, subditine (1), demonstrated the most potent cell growth inhibition activity and selective against LNCaP with an IC50 of 12.2460.19 mM and …
Kingianic Acids A–G, Endiandric Acid Analogues From Endiandra Kingiana, Kok Hoong Leong
Kingianic Acids A–G, Endiandric Acid Analogues From Endiandra Kingiana, Kok Hoong Leong
Kok Hoong Leong
A phytochemical investigation of the methanolic extract of the bark of Endiandra kingiana led to the isolation of seven new tetracyclic endiandric acid analogues, kingianic acids A–G (1–7), together with endiandric acid M (8), tsangibeilin B (9) and endiandric acid (10). Their structures were determined by 1D- and 2D-NMR analysis in combination with HRMS experiments. The structure of compounds 9 and 10 were confirmed by single-crystal X-ray diffraction analysis. These compounds were screened for Bcl-xL and Mcl-1 binding affinities and cytotoxic activity on various cancer cell lines. Compound 5 showed moderate cytotoxic activity against human colorectal adeno-carcinoma (HT-29) and lung …
Induction Of Apoptosis In Melanoma A375 Cells By A Chloroform Fraction Of Centratherum Anthelminticum (L.) Seeds Involves Nf-Kappab, P53 And Bcl-2-Controlled Mitochondrial Signaling Pathways, Kok Hoong Leong
Kok Hoong Leong
Centratherum anthelminticum (L.) Kuntze (scientific synonyms: Vernonia anthelmintica; black cumin) is one of the ingredients of an Ayurvedic preparation, called “Kayakalp”, commonly applied to treat skin disorders in India and Southeast Asia. Despite its well known anti-inflammatory property on skin diseases, the anti-cancer effect of C. anthelminticum seeds on skin cancer is less documented. The present study aims to investigate the anti-cancer effect of Centratherum anthelminticum (L.) seeds chloroform fraction (CACF) on human melanoma cells and to elucidate the molecular mechanism involved.
Cytotoxic And Antioxidant Compounds From The Stem Bark Of Goniothalamus Tapisoides Mat Salleh, Kok Hoong Leong
Cytotoxic And Antioxidant Compounds From The Stem Bark Of Goniothalamus Tapisoides Mat Salleh, Kok Hoong Leong
Kok Hoong Leong
Eleven compounds:goniomicin A (1), goniomicin B (2), goniomicin C (3), goniomicin D (4), tapisoidin (5), goniothalamin (6), 9-deoxygoniopypyrone (7), pterodondiol (8), liriodenine (9), benzamide (10) and cinnamic acid (11), were isolated from the stem bark of Goniothalamus tapisoides. All compounds were identified by spectroscopic analysis and, for known compounds, by comparison with published data. Goniothalamin (6) exhibited mild cytotoxic activity towards a colon cancer cell line (HT-29), with an IC50value of 64.17 ± 5.60 μM. Goniomicin B (2) give the highest antioxidant activity in the DPPH assay among all compounds tested, with an IC50 of 0.207 μM.
Triterpenes And Steroids From The Leaves Of Aglaia Exima (Meliaceae), Kok Hoong Leong
Triterpenes And Steroids From The Leaves Of Aglaia Exima (Meliaceae), Kok Hoong Leong
Kok Hoong Leong
A study on the leaves of Aglaia exima led to the isolation of one new and seven known compounds: six triterpenoids and two steroids. Their structures were elucidated and analyzed mainly by using spectroscopic methods; 1D and 2D NMR, mass spectrometry, UV spectrometry and X-ray. All the triterpenoids and steroids were measured in vitro for their cytotoxic activities against eight cancer cell lines; lung (A549), prostate (DU-145), skin (SK-MEL-5), pancreatic (BxPC-3), liver (Hep G2), colon (HT-29), breast (MCF-7) and (MDA-MB-231). The new cycloartane triterpenoid, 24(E)-cycloart-24-ene-26-ol-3-one 1, showed potent cytotoxic activity against colon (HT-29) cancer cell line (IC50 11.5 μM).
Spectaflavoside A, A New Potent Iron Chelating Dimeric Flavonol Glycoside From The Rhizomes Of Zingiber Spectabile Griff, Kok Hoong Leong
Spectaflavoside A, A New Potent Iron Chelating Dimeric Flavonol Glycoside From The Rhizomes Of Zingiber Spectabile Griff, Kok Hoong Leong
Kok Hoong Leong
The rhizomes of Zingiber spectabile yielded a new dimeric flavonol glycoside for which the name kaempferol-3-O-(4″-O-acetyl)-α-l-rhamnopyranoside-(I-6,II-8)-kaempferol-3-O-(4″-O-acetyl)-α-l-rhamnopyranoside; spectaflavoside A (1) was proposed, along with kaempferol and its four acetylrhamnosides (2–6), demethoxycurcumin (7) and curcumin (8). The structure of spectaflavoside A was elucidated by spectroscopic methods including, 1D and 2D NMR techniques. This is the first report on the occurrence of a dimeric flavonol glycoside in the Zingiberaceae and the second in nature. Spectaflavoside A was found to be a potent iron chelating agent.
Erectogenic Effects Of Clerodendron Capitatum: Involvement Of Phosphodiesterase Type-5 Inhibition, Dr Syam Mohan
Erectogenic Effects Of Clerodendron Capitatum: Involvement Of Phosphodiesterase Type-5 Inhibition, Dr Syam Mohan
Syam Mohan
No abstract provided.
Clausena Excavata Burm. F. (Rutaceae): A Review Of Its Traditional Uses, Pharmacological And Phytochemical Properties., Dr Syam Mohan
Clausena Excavata Burm. F. (Rutaceae): A Review Of Its Traditional Uses, Pharmacological And Phytochemical Properties., Dr Syam Mohan
Syam Mohan
The present review describes the morphological, traditional, phytochemical and pharmacological aspects of Clausena excavata (Burm. f., Rutaceae). The plant grows wild in the tropical and subtropical parts of Asia and represents one of the richest sources of medicinal natural products. It is intensively used currently in traditional medicine for treatment of different illnesses. One part or a mixture of two or more parts of the plant in the form of powder or liquid doze is usually given to the patient. Phytochemically, many pure compounds from several secondary metabolite groups of C. excavata have been isolated from different parts of the …
Chloroform Fraction Of Centratherum Anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha And Exhibits Pleotropic Bioactivities: Inhibitory Role In Human Tumor Cells, Dr Syam Mohan
Syam Mohan
We investigated the antioxidant potential, cytotoxic effect, and TNF-α inhibition activity with NF-κB activation response in a chloroformfraction of Centratherum anthelminticum seeds (CACF). The antioxidant property of CACF was evaluated with DPPH, ORAC, and FRAP assays, which demonstrated significant antioxidant activity. The cytotoxicity of CACF was tested using theMTT assay; CACF effective inhibitory concentrations (IC50) for A549, PC-3, MCF-7, and WRL-68 cells were 31.42 ± 5.4, 22.61 ± 1.7, 8.1±0.9, and 54.93±8.3 μg/mL, respectively. CACF effectively and dose-dependently inhibited TNF-α release, in vitro and in vivo. CACF inhibited TNF-α secretion in stimulated RAW264.7macrophage supernatants with an IC50 of 0.012 μg/mL, …
In Vitro Anti-Inflammatory, Cytotoxic And Antioxidant Activities Of Boesenbergin A, A Chalcone Isolated From Boesenbergia Rotunda (L.) (Fingerroot), Dr Syam Mohan
Syam Mohan
The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as …
Synthesis Of Chalcones With Anticancer Activities., Dr Syam Mohan
Synthesis Of Chalcones With Anticancer Activities., Dr Syam Mohan
Syam Mohan
Several chalcones were synthesized and their in vitro cytotoxicity against various human cell lines, including human breast adenocarcinoma cell line MCF-7, human lung adenocarcinoma cell line A549, human prostate cancer cell line PC3, human adenocarcinoma cell line HT-29 (colorectal cancer) and human normal liver cell line WRL-68 was evaluated. Most of the compounds being active cytotoxic agents, four of them with minimal IC50 values were chosen and studied in detail with MCF-7 cells. The compounds 1, 5, 23, and 25 were capable in eliciting apoptosis in MCF-7 cells as shown by multiparameter cytotoxicity assay and caspase-3/7, -8, and -9 activities …
Cucurbitacin L 2-O- Glucoside Demonstrates Apoptogenesis In Colon Adenocarcinoma Cells (Ht-29): Involvement Of Reactive Oxygen And Nitrogen Species Regulation, Dr Syam Mohan
Syam Mohan
Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results …
Dentatin Induces Apoptosis In Prostate Cancer Cells Via Bcl-2, Bcl-Xl, Survivin Downregulation, Caspase-9, -3/7 Activation And Nf-Kb Inhibition., Dr Syam Mohan
Syam Mohan
This study was set to investigate antiproliferative potential of dentatin (a natural coumarin isolated from Clausena excavata Burm. F) against prostate cancer and to delineate the underlying mechanism of action. Treatment with dentatin dose-dependently inhibited cell growth of PC-3 and LNCaP prostate cancer cell lines, whereas it showed less cytotoxic effects on normal prostate epithelial cell line (RWPE-1). The inhibitory effect of dentatin on prostate cancer cell growth was due to induction of apoptosis as evidenced by Annexin V staining and cell shrinkage. We found that dentatin-mediated accumulation of reactive oxygen species (ROS) and downregulated expression levels of antiapoptotic molecules …
Lectin-Functionalized Carboxymethylated Kappa-Carrageenan Microparticles For Oral Insulin, Kok Hoong Leong
Lectin-Functionalized Carboxymethylated Kappa-Carrageenan Microparticles For Oral Insulin, Kok Hoong Leong
Kok Hoong Leong
We hypothesized that pH-responsive carboxymethylated kappa-carrageenan microparticles could protect entrapped oral insulin from acidic and proteolytic degradation in the gastrointestinal tract. Therefore, the objectives of this study were to prepare and characterize insulin entrapped in lectin-functionalized carboxymethylated kappa-carrageenan microparticles and to evaluate their therapeutic efficacy in vitro and in vivo. The encapsulation of insulin was performed using an ionic gelation technique and was optimized to give an encapsulation efficiency of 94.2 ± 2.6% and a drug-loading capacity of 13.5 ± 0.4%. The microparticles were further surface-lectin-functionalized for improved intestinal mucoadhesiveness. The oral administration of insulin entrapped in the microparticles led …
Flag On The Play: The Ninth Circuit’S End-Run Around Implied Rights Of Action Runs Afoul In County Of Santa Clara V. Astra, Usa Inc., Brooke Burns
Brooke Burns
In County of Santa Clara v. Astra, USA Inc., the Ninth Circuit held that a third party was entitled to bring a private right of action for breach of contract under federal common law, even though the governing statute neither expressly nor impliedly provides for this right. Because the Supreme Court has increasingly limited the ability of a third party to bring an implied right of action claim, private parties have pursued third party beneficiary claims instead. As a result, a considerable circuit split has resulted as to whether federal common law provides a private right of action to a …
Turning Packaging Into Profit, Rihaz Z. Chughatta
Turning Packaging Into Profit, Rihaz Z. Chughatta
Rihaz Z Chughatta
Packaging can be an expensive function to maintain, but it doesn’t have to be. In fact, Packaging can be a source of income for a company. More accurately, what if you took a look at your company’s Profit and Loss and saw Packaging as a negative expense? That’s right, you can turn packaging into profit for your company. How do you accomplish this feat? Glad you asked … read on to find three simple ways to start this process (and satisfy your curiosity) …
Identification Of 2-Aminothiazole-4-Carboxylate Derivatives Active Against Mycobacterium Tuberculosis H37rv And The B-Ketoacyl-Acp Synthase Mtfabh, Philadelphia University
Identification Of 2-Aminothiazole-4-Carboxylate Derivatives Active Against Mycobacterium Tuberculosis H37rv And The B-Ketoacyl-Acp Synthase Mtfabh, Philadelphia University
Philadelphia University, Jordan
No abstract provided.
21st Century Healthcare - A New Scenario Needs New Rules Of The Game, Mian Atif Saeed
21st Century Healthcare - A New Scenario Needs New Rules Of The Game, Mian Atif Saeed
Mian Atif Saeed
We are witnessing a changing paradigm of healthcare sector in view increasing pressure on governments to provide free healthcare to citizens and increasing cost-consciousness of governments/payers to address this public issue. This changing paradigm in healthcare requirements requires a holistic review of the legislative framework in which industry operates. All stakeholders and all applicable regulatory and legal frameworks need to be appraised in order to address the requirements of 21st century healthcare. Drug development is very risky, costly and lengthy process and inventors and investor deserve financial gains for their efforts. Pharmaceutical industry requires the money to keep investing back …
Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr
Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr
Maria Cekanova MS, RNDr, PhD
No abstract provided.
Brivaracetam: A Rational Drug Discovery Success Story, Michael Rogawski
Brivaracetam: A Rational Drug Discovery Success Story, Michael Rogawski
Michael A. Rogawski
Levetiracetam, the alpha-ethyl analogue of the nootropic piracetam, is a widely used antiepileptic drug (AED) that provides protection against partial seizures and is also effective in the treatment of primary generalized seizure syndromes including juvenile myoclonic epilepsy. Levetiracetam was discovered in 1992 through screening in audiogenic seizure susceptible mice and, 3 years later, was reported to exhibit saturable, stereospecific binding in brain to a approximately 90 kDa protein, later identified as the ubiquitous synaptic vesicle glycoprotein SV2A. A large-scale screening effort to optimize binding affinity identified the 4-n-propyl analogue, brivaracetam, as having greater potency and a broadened spectrum of activity …
Outsourcing The Packaging Function, Rihaz Z. Chughatta
Outsourcing The Packaging Function, Rihaz Z. Chughatta
Rihaz Z Chughatta
If you are currently working in the packaging department of a major corporation in the pharmaceutical, food or consumer products industry, you have probably been exposed to some form of outsourcing, which is a global trend that has emerged over the past decade, and continues to evolve, within the packaging field.
Dohad, Influenza And Economists, Stephen E. Snyder
Dohad, Influenza And Economists, Stephen E. Snyder
stephen e snyder
The Developmental Origin of Disease and Health hypothesizes that the early-life, including pre-natal, shocks to health. affects individuals’ later-life health and mortality. Following a line of research established by Doug Almond (2006), we examine whether the 1918 influenza epidemic is a health shock which is orthogonal to chronic health status. Almond, however, does not present results on mortality rates. Our findings are that 1) cross sectional data does not exist which would allow us to treat the influenza epidemic as a field experiment with state-by-state variation, and that when we use what data exists, controlling for geographic variation in health, …
Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller
Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller
Maria Cekanova MS, RNDr, PhD
BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six small …
The Neurobiology Of Antiepileptic Drugs For The Treatment Of Nonepileptic Conditions, Michael A. Rogawski, Wolfgang Löscher
The Neurobiology Of Antiepileptic Drugs For The Treatment Of Nonepileptic Conditions, Michael A. Rogawski, Wolfgang Löscher
Michael A. Rogawski
Antiepileptic drugs (AEDs) are commonly prescribed for nonepileptic conditions, including migraine headache, chronicneuropathic pain, mood disorders, schizophrenia and various neuromuscular syndromes. In many of these conditions, as in epilepsy, the drugs act by modifying the excitability of nerve (or muscle) through effects on voltage-gated sodium and calciumchannels or by promoting inhibition mediated by γ-aminobutyric acid (GABA) A receptors. In neuropathic pain, chronic nerveinjury is associated with the redistribution and altered subunit compositions of sodium and calcium channels that predisposeneurons in sensory pathways to fire spontaneously or at inappropriately high frequencies, often from ectopic sites. AEDs maycounteract this abnormal activity by …
Multiple Luteinizing Hormone Receptor (Lhr) Protein Variants, Interspecies Reactivity Of Anti-Lhr Mab Clone 3b5, Subcellular Localization Of Lhr In Human Placenta, Pelvic Floor And Brain, And Possible Role For Lhr In The Development Of Abnormal Pregnancy, Pelvic Floor Disorders And Alzheimer's Disease, A Bukovsky, K Indrapichate, H Fujiwara, Maria Cekanova Ms, Rndr, Phd, Me Ayala, R Dominguez, Mr Caudle, J Wimalsena, Rf Elder, P Copas, Jf Foster, Ri Fernando, Dc Henley, Nb Upadhyaya
Multiple Luteinizing Hormone Receptor (Lhr) Protein Variants, Interspecies Reactivity Of Anti-Lhr Mab Clone 3b5, Subcellular Localization Of Lhr In Human Placenta, Pelvic Floor And Brain, And Possible Role For Lhr In The Development Of Abnormal Pregnancy, Pelvic Floor Disorders And Alzheimer's Disease, A Bukovsky, K Indrapichate, H Fujiwara, Maria Cekanova Ms, Rndr, Phd, Me Ayala, R Dominguez, Mr Caudle, J Wimalsena, Rf Elder, P Copas, Jf Foster, Ri Fernando, Dc Henley, Nb Upadhyaya
Maria Cekanova MS, RNDr, PhD
Distinct luteinizing hormone receptor (LHR) protein variants exist due to the posttranslational modifications. Besides ovaries, LHR immunoreactivity (LHRI) was also found in other tissues, such as the brain, fallopian tube, endometrium, trophoblast and resident tissue macrophages. The 3B5 mouse monoclonal antibody was raised against purified rat LHR. In rat, porcine and human ovaries, the 3B5 identified six distinct LHR bands migrating at approximately 92, 80, 68, 59, 52 and 48 kDa. Characteristic LHRI was detected in rat, human and porcine corpora lutea. During cellular differentiation, subcellular LHR distribution changed from none to granular cytoplasmic, perinuclear, surface, nuclear and no staining. …
Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder
Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder
Maria Cekanova MS, RNDr, PhD
Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …
Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder
Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder
Maria Cekanova MS, RNDr, PhD
During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …