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Full-Text Articles in Animal Experimentation and Research

Recent Efforts To Elucidate The Scientific Validity Of Animal-Based Drug Tests By The Pharmaceutical Industry, Pro-Testing Lobby Groups, And Animal Welfare Organisations, Jarrod Bailey Mar 2019

Recent Efforts To Elucidate The Scientific Validity Of Animal-Based Drug Tests By The Pharmaceutical Industry, Pro-Testing Lobby Groups, And Animal Welfare Organisations, Jarrod Bailey

Validation of Animal Experimentation

Background: Even after several decades of human drug development, there remains an absence of published, substantial, comprehensive data to validate the use of animals in preclinical drug testing, and to point to their predictive nature with regard to human safety/toxicity and efficacy. Two recent papers, authored by pharmaceutical industry scientists, added to the few substantive publications that exist. In this brief article, we discuss both these papers, as well as our own series of three papers on the subject, and also various views and criticisms of lobby groups that advocate the animal testing of new drugs.

Main text: We ...


Changes In Expression Of The Autophagy-Related Genes Microtubule-Associated Protein 1 Light Chain 3Β And Autophagy Related 7 In Skeletal Muscle Of Fattening Japanese Black Cattle: A Pilot Study, Tomonori Nakanishi, Tadaaki Tokunaga, Takafumi Ishida, Ikuo Kobayashi, Yuta Katahama, Azusa Yano, Laurie Erickson, Satoshi Kawahara Jan 2019

Changes In Expression Of The Autophagy-Related Genes Microtubule-Associated Protein 1 Light Chain 3Β And Autophagy Related 7 In Skeletal Muscle Of Fattening Japanese Black Cattle: A Pilot Study, Tomonori Nakanishi, Tadaaki Tokunaga, Takafumi Ishida, Ikuo Kobayashi, Yuta Katahama, Azusa Yano, Laurie Erickson, Satoshi Kawahara

Hebrew Theological College Publications and Research

Objective

Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain 3β (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth.

Methods

Six castrated Japanese Black cattle (initial body weight: 503±20 kg) were enrolled ...


Effect Of Group Size On The Activity Budget Of Two Captive Chimpanzees (Pan Troglodytes), Kaeley Sullins Jan 2019

Effect Of Group Size On The Activity Budget Of Two Captive Chimpanzees (Pan Troglodytes), Kaeley Sullins

All Master's Theses

Captive facilities housing chimpanzees are required to provide adequate care and provisions such as dietary, social, and environmental enrichment to promote the psychological well-being of the apes in their care. Chimpanzees are social creatures and changes in groups as well as relocation to a facility with new social partners, can impact each individual chimpanzee’s welfare. By tracking each chimpanzee’s activity budgets, managers can assess welfare and make improvements or adjustments if necessary. I looked at the activity budgets of two captive chimpanzees after the death of a group member and the two chimpanzees’ subsequent relocation to a novel ...


Study Of Three Potential Variables That May Impact The Maximum Shear Force And Shear Variation During The Growth And Transport Of Mixed Sex Broilers Grown In Commercial Poultry Houses, Mark Christie Dec 2018

Study Of Three Potential Variables That May Impact The Maximum Shear Force And Shear Variation During The Growth And Transport Of Mixed Sex Broilers Grown In Commercial Poultry Houses, Mark Christie

Theses and Dissertations

Temperature conditions during the transportation to the processing facility of commercial broilers have a direct impact on the meat texture, specifically maximum shear force and shear variation of cooked breast meat. Analysis of a calendar year of flock data (N=346), reveals that the largest impact of transport conditions, specifically temperature, is on the standard deviation of the flocks range of cooked shear values. The optimal transport temperature that yields broiler breast meat with average flock minimum shear and standard deviation values is approximately 60°F. The shear values for flocks at or near 60°F are on average 1 ...


Optimizing Methods To Determine Metabolizable Energy Values Of Feed Ingredients For Broilers, Skyler P. West Dec 2018

Optimizing Methods To Determine Metabolizable Energy Values Of Feed Ingredients For Broilers, Skyler P. West

Theses and Dissertations

Determination of metabolizable energy (ME) and amino acid (AA) digestibility values of single feed indigents continues to be two of the most important aspects for successful least-cost poultry feed formulation. It would be advantageous if a common diet type could be utilized to determine both ME and AA digestibility values of feed ingredients within a single assay. Two experiments were conducted to evaluate the effect of basal diet type and excreta collection method on the ME value of single feed ingredients determined in broiler chicks using the regression method. In experiment 1, the objective was to investigate the influence of ...


No Evidence For Kin Protection In The Expression Of Sickness Behaviors In House Mice, Patricia C. Lopes, Per Block, Alice Pontiggia, Anna K. Lindholm, Barbara König Nov 2018

No Evidence For Kin Protection In The Expression Of Sickness Behaviors In House Mice, Patricia C. Lopes, Per Block, Alice Pontiggia, Anna K. Lindholm, Barbara König

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

When infected, animals change their behaviors in several ways, including by decreasing their activity, their food and water intake, and their interest in social interactions. These behavioral alterations are collectively called sickness behaviors and, for several decades, the main hypotheses put forward to explain this phenomenon were that engaging in sickness behaviors facilitated the fever response and improved the likelihood of host survival. However, a new hypothesis was recently proposed suggesting that engaging in sickness behaviors may serve to protect kin. We tested this kin protection hypothesis by combining a field and a laboratory experiment in house mice. In both ...


Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater Nov 2018

Development Of A Pd-L1 Pet Imaging Biomarker, Caleb Jack Bridgwater

Posters-at-the-Capitol

Immunotherapy strategies are very promising treatments for cancer patients. Specifically, Immune checkpoint inhibitor therapy focusing on the PD-1/PD-L1 pathway shows long-lasting positive results in many cancer patients. Unfortunately, not all the patients can benefit from this highly effective treatment. Hence, there is a great need for predictive biomarkers. Immunohistochemical (IHC) staining has been used as a way of predicting patient response, yet shows many problems. For example, IHC utilizes an invasive biopsy and sample fixing, which creates an incomplete and delayed picture of the patient’s biochemistry and the tumor microenvironment, consequently ignoring metastases.

The purpose of this study ...


Cannabinoid Transmission In The Basolateral Amygdala Modulates Prefrontal Cortex And Ventral Hippocampal Activity, Brian J. Pereira Oct 2018

Cannabinoid Transmission In The Basolateral Amygdala Modulates Prefrontal Cortex And Ventral Hippocampal Activity, Brian J. Pereira

Electronic Thesis and Dissertation Repository

The cannabinoid system is important for maintaining neuron-to-neuron communication within the mammalian brain. One of the most commonly used substances to alter the cannabinoid system is cannabis. Individuals who are exposed to cannabis report having dissociable effects; both positive and negative. High amounts of THC have been commonly associated with the negative effects of cannabis, whereas CBD can be used to counter these. Pre-clinical evidence suggests that the combination of the two compounds can produce a therapeutic benefit for individuals who are susceptible to the effects of THC. The present study investigates whether the combination of THC+CBD can prevent ...


Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low Aug 2018

Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low

The Summer Undergraduate Research Fellowship (SURF) Symposium

In patients over the age of 65 especially, bone fractures represent a significant disease burden. Non-invasive drug therapies are not available for bone fractures which represents a problem for this population. Vasoactive intestinal peptide (VIP) and Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), two neuromodulator peptides in the glucagon superfamily, have demonstrated positive regulation of osteoblast proliferation and activity. Using acidic oligopeptides, we have developed ligands that target to and accumulate at fracture sites. These targeting ligands can be synthesized in sequence with bone anabolic peptides to minimize off target effects and increase potency at the fracture site to create safer and ...


Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan Jul 2018

Investigation Of Zebrafish Larvae Behavior As Precursor For Suborbital Flights: Feasibility Study, Pedro Llanos, Kristina Andrijauskaite, Mark Rubinstein, Sherine S.L. Chan

Pedro J. Llanos (www.AstronauticsLlanos.com)


Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light ...


Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk Jul 2018

Identifying The 5’End Of The Camta1 Genes In Zebrafish, Morgan Fronk

Undergraduate Honors Theses

Congenital heart diseases (CHDs) are a significant cause of infant death and are frequently caused by mutations in transcription factors. Camta1 (calmodulin binding transcription activator 1) is a transcription factor that has been proposed as a modulator in embryonic heart development and a possible cause of CHDs. The only other known member of its family in vertebrates is involved in activating a hypertrophy gene program in adult heart failure. Unlike camta2, camta1 is expressed in the embryonic heart during heart looping. However, few studies have been done on camta1. In zebrafish, there are two camta1 ohnologs (homologs created through a ...


Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie Jul 2018

Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie

Masters Theses & Specialist Projects

While substrate-borne vibrations are utilized by different reptile species, true conspecific communication via biotremors has not yet been demonstrated in reptiles. This study follows a preliminary report that the veiled chameleon (Chamaeleo calyptratus) could produce biotremors in communicative contexts. I tested chameleon behavioral sensitivity to vibrations by placing them on a dowel attached to a shaker emitting vibrations of 25, 50, 150, 300, and 600 Hz and then measured their changes in velocity before and after the stimulus. I then paired chameleons in various social contexts [anthropogenic disturbance (human disruption of animal); dominance (malemale; female-female C. calyptratus); courtship (male-female C ...


Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins Jun 2018

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas ...


The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage May 2018

The Wide Utility Of Rabbits As Models Of Human Diseases, Pedro J. Esteves, Yuxing Chen, Shuying Liu, Shan Lu, Michael Vaine, Shixia Wang, Rose Mage

Open Access Articles

Studies using the European rabbit Oryctolagus cuniculus contributed to elucidating numerous fundamental aspects of antibody structure and diversification mechanisms and continue to be valuable for the development and testing of therapeutic humanized polyclonal and monoclonal antibodies. Additionally, during the last two decades, the use of the European rabbit as an animal model has been increasingly extended to many human diseases. This review documents the continuing wide utility of the rabbit as a reliable disease model for development of therapeutics and vaccines and studies of the cellular and molecular mechanisms underlying many human diseases. Examples include syphilis, tuberculosis, HIV-AIDS, acute hepatic ...


Juvenile Amphibian Response To Oak And Maple Leaf Litter, Benjamin Breslau May 2018

Juvenile Amphibian Response To Oak And Maple Leaf Litter, Benjamin Breslau

Honors Scholar Theses

The composition of tree species within New England forests has changed significantly in recent decades, with an increase in maple (Acer spp.) abundance and a decrease in oak (Quercus spp.) abundance. Changing forest structure results in changing leaf litter composition of the forest floor, which influences the ground-dwelling amphibians that live in the litter. To better understand how changes to forest composition alters amphibian habitat quality, we recorded the growth and survival of 27 juvenile wood frogs (Lithobates sylvaticus or Rana sylvatica) and 27 juvenile American toads (Anaxyrus americanus) in response to leaf litter type. Between early August and late ...


The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd May 2018

The Effect Of Diet On Craniofacial Growth In Osteogenesis Imperfecta Mouse Model, Summer H. Ladd

Theses and Dissertations

Osteogenesis imperfecta (OI, or “brittle bone disease”) is a rare disorder that is caused by genetic point mutations (COL1A1/COL1A2) that affect type 1 collagen. In OI type III (severe) patients, limb bones are more susceptible to skeletal fractures and the bones of the craniofacial region are underdeveloped. Some OI type III patients also suffer from dental malocclusions or fractures (dentinogenesis imperfecta, DI). The goals of this project are 1) to describe the facial phenotype in an OI mouse model, to see if this model can be used to test potential behavioral and pharmaceutical interventions; and 2) to determine if ...


Pyruvate Kinase Isoform M2 Influences Autophagy And Related Processes In Hepatocellular Carcinoma Cells, Matthew Lin May 2018

Pyruvate Kinase Isoform M2 Influences Autophagy And Related Processes In Hepatocellular Carcinoma Cells, Matthew Lin

University Scholar Projects

Hepatocellular carcinoma (HCC) is the most common form of liver cancer that affects ~14 million people in the world. Like all cancers, HCC is a disease that arises from unstinted cellular growth initiated by genetic alterations, metabolic changes, and dysregulation in key cellular pathways. Of interest is the relationship between metabolism and cell proliferation/degradation for therapeutic targeting. Pyruvate kinase M2 is a dimeric, glycolytically inactive isoform of the final enzyme involved in glycolysis, that is often upregulated in cancerous tissue. It is thought that the enzymatic function of PKM2 outside of glycolysis contributes to the biosynthesis of anabolic intermediates ...


Effects Of Hypoxia On Swimming And Sensing In A Weakly Electric Fish, Kerri Lynn Ackerly, Rüdiger Krahe, Christopher P. Sanford, Lauren J. Chapman May 2018

Effects Of Hypoxia On Swimming And Sensing In A Weakly Electric Fish, Kerri Lynn Ackerly, Rüdiger Krahe, Christopher P. Sanford, Lauren J. Chapman

Faculty Publications

Low dissolved oxygen (hypoxia) can severely limit fish performance, especially aerobically expensive behaviours including swimming and acquisition of sensory information. Fishes can reduce oxygen requirements by altering these behaviours under hypoxia, but the underlying mechanisms can be difficult to quantify. We used a weakly electric fish as a model system to explore potential effects of hypoxia on swim performance and sensory information acquisition, which enabled us to non-invasively record electric signalling activity used for active acquisition of sensory information during swimming. To quantify potential effects of hypoxia, we measured critical swim speed (Ucrit) and concurrent electric signalling activity under highand ...


Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff Apr 2018

Respiratory Disease, Behavior, And Survival Of Mountain Goat Kids, Julie A. Blanchong, Christopher A. Anderson, Nicholas Clark, Robert W. Klaver, Paul J. Plummer, Mike Cox, Caleb Mcadoo, Peregrine L. Wolff

Natural Resource Ecology and Management Publications

Bacterial pneumonia is a threat to bighorn sheep (Ovis canadensis) populations. Bighorn sheep in the East Humboldt Mountain Range (EHR), Nevada, USA, experienced a pneumonia epizootic in 2009–2010. Testing of mountain goats (Oreamnos americanus) that were captured or found dead on this range during and after the epizootic detected bacteria commonly associated with bighorn sheep pneumonia die‐offs. Additionally, in years subsequent to the bighorn sheep epizootic, the mountain goat population had low kid:adult ratios, a common outcome for bighorn sheep populations that have experienced a pneumonia epizootic. We hypothesized that pneumonia was present and negatively affecting mountain ...


Emptying And Refilling Of Slime Glands In Atlantic (Myxine Glutinosa) And Pacific (Eptatretus Stoutii) Hagfishes, Sarah Schorno, Todd E. Gillis, Douglas S. Fudge Apr 2018

Emptying And Refilling Of Slime Glands In Atlantic (Myxine Glutinosa) And Pacific (Eptatretus Stoutii) Hagfishes, Sarah Schorno, Todd E. Gillis, Douglas S. Fudge

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Hagfishes are known for their unique defensive slime, which they use toward off gill-breathing predators. Although much is known about the slime cells (gland thread cells and gland mucous cells), little is known about how long slime gland refilling takes, or how slime composition changes with refilling or repeated stimulation of the same gland. Slime glands can be individually electrostimulated to release slime, and this technique was used to measure slime gland refilling times for Atlantic and Pacific hagfish. The amount of exudate produced, the composition of the exudate and the morphometrics of slime cells were analyzed during refilling, and ...


Whole Tart Cherry Improves Disease Activity Index In Rat Model Of Dextran Sulfate Solution – Induced Ulcerative Colitis By Downregulating The Janus 1 And Janus 3 Kinases And Tnf - Alpha, Johana Alexandra Coronel Apr 2018

Whole Tart Cherry Improves Disease Activity Index In Rat Model Of Dextran Sulfate Solution – Induced Ulcerative Colitis By Downregulating The Janus 1 And Janus 3 Kinases And Tnf - Alpha, Johana Alexandra Coronel

LSU Master's Theses

Ulcerative colitis (UC) is a disabling inflammatory bowel disease. UC is characterized by chronic, relapsing inflammation of the colon and rectum. Current treatments such as monoclonal antibodies against TNF-α, IL-6, IL-12/p40, adhesion molecules, Janus kinases (JAK) inhibitors have side effects or lose their effects over time. Alternative approaches with fewer side effects for patients are needed. Montmorency tart cherries (Prunus cerasus) are a good source of anti-inflammatory flavonoids. We hypothesized that regular consumption whole tart cherry (TC) standardized to its major anthocyanin content, namely cyanidin-3-glucosyl-rutinoside, would be effective in reducing inflammation in UC. The aim of this research was ...


Developing New Apparatus For In-Situ Vermicomposting And Varying Diet Parameters To Understand Optimal Conditions For Soil Fertilization., Nataliya Ryzhenko Mar 2018

Developing New Apparatus For In-Situ Vermicomposting And Varying Diet Parameters To Understand Optimal Conditions For Soil Fertilization., Nataliya Ryzhenko

Lesley University Community of Scholars Day

Vermicomposting is the process of decomposing vegetable and other food waste using earthworms.

We investigate apparatus for in-situ vermicomposting and vary diet parameters to understand optimal conditions for soil fertilization.

As a pilot project, we created a small in-situ vermicomposting apparatus which can be used in a home or at a small farm. In this setting, we need to ensure that the setup does not produce repulsive odor, since it is meant to be kept indoors. Also, the setup should be easily maintained: the food waste easily added, and the bedding kept moist.

Based on results seen, in-situ vermicomposting farm ...


Addressing The Challenges Of Conducting Observational Studies In Sheep Abattoirs, Elyssa Payne, Melissa Starling, Paul Mcgreevy Feb 2018

Addressing The Challenges Of Conducting Observational Studies In Sheep Abattoirs, Elyssa Payne, Melissa Starling, Paul Mcgreevy

Paul McGreevy, Ph.D.

The competing needs of maintaining productivity within abattoirs, and maintaining high standards of animal welfare, provide fertile grounds for applied research in animal behavior. However, there are challenges involved in capturing useful behavioral data from the supply chain (from paddock to processing plant). The challenges identified in this report are based on a review of the scientific literature as well as field study observations. This article describes those challenges as they relate to collecting behavioral data on livestock-herding dogs, humans and livestock as they interact in abattoirs, and provides insights and recommendations for others embarking on animal studies in confined ...


The Role Of Ripk1 Kinase Activity In Regulating Inflammation And Necroptotic Death, Matija Zelic Jan 2018

The Role Of Ripk1 Kinase Activity In Regulating Inflammation And Necroptotic Death, Matija Zelic

GSBS Dissertations and Theses

Necroptosis, a type of regulated necrotic cell death, involves cell membrane permeabilization and has been implicated in various acute and chronic pro-inflammatory diseases, including ischemia-reperfusion injury and neurodegenerative diseases. By using in vitro reconstitution studies and a chemical inhibitor, the kinase activity of the serine/threonine kinase RIPK1 had been shown to regulate necroptotic signaling downstream of TNF and Toll-like receptors (TLRs). To investigate the contribution of RIPK1 kinase activity to inflammation and necroptosis in vivo, we generated kinase inactive RIPK1 knock-in mice. Utilizing fibroblasts and macrophages from these mice, we demonstrate that RIPK1 kinase activity is required for necroptotic ...


Information Retrieval For Systematic Reviews In Food And Feed Topics: A Narrative Review, Hannah Wood, Annette O'Connor, Jan Sargeant, Julie Glanville Jan 2018

Information Retrieval For Systematic Reviews In Food And Feed Topics: A Narrative Review, Hannah Wood, Annette O'Connor, Jan Sargeant, Julie Glanville

Veterinary Diagnostic and Production Animal Medicine Publications

Introduction Systematic review methods are now being used for reviews of food production, food safety and security, plant health, and animal health and welfare. Information retrieval methods in this context have been informed by human healthcare approaches and ideally should be based on relevant research and experience.

Objective This narrative review seeks to identify and summarise current research-based evidence and experience on information retrieval for systematic reviews in food and feed topics.

Methods MEDLINE (Ovid), Science Citation Index (Web of Science) and ScienceDirect (http://www.sciencedirect.com/) were searched in 2012 and 2016. We also contacted topic experts and undertook ...


Disarming “Nature” As A Weapon: A Queer Ecosemiotic Reimagining Of Futurity And Environmental Ethics Through Memoir, Sam Lauer Jan 2018

Disarming “Nature” As A Weapon: A Queer Ecosemiotic Reimagining Of Futurity And Environmental Ethics Through Memoir, Sam Lauer

Master’s Theses

In this thesis, I posit that the need for an active, conscious, and radical queering of ecocriticism as a literary and cultural theory has arisen in light of the postmodern problematization of “nature” and the “natural,” along with the queerness of society, culture, and science. The way we understand “nature” (in life and in texts), whether of physical environments, inherent selfhood, or normalcy, begs to be appropriately informed by discourses and realities of queerness in order for both social and environmental healing to take place. I have analyzed three works of queer creative nonfiction—memoirs—to illuminate the ways in ...


Prior Voluntary Wheel Running Prevents Memory Deficits And Enhanced Neuropathic Pain Presenting After Morphine Dosing Ceases, Nathan Plattner Jan 2018

Prior Voluntary Wheel Running Prevents Memory Deficits And Enhanced Neuropathic Pain Presenting After Morphine Dosing Ceases, Nathan Plattner

Undergraduate Honors Theses

Clinical research studies show that chronic pain is a prevalent, difficult affliction to manage. The most common management for chronic pain is opioids, which research is showing to paradoxically induce persistent, worsened pain states as well as potentially having negative effects on memory. The necessity for understanding chronic pain and drug management options is apparent, and research is aiming to do just that. Exercise is one potential preventative measure or post hoc treatment possibility for chronic pain, as abundant research has shown. Knowledge of the interactions between exercise, morphine dosing, and peripheral nerve injury is nonexistent. As such, we desired ...


Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom Jan 2018

Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom

Food Science and Human Nutrition Publications

Water-soluble proteins extracted from two species of grasshoppers, Patanga succincta (WSPP) and Chondracris roseapbrunner (WSPC), were characterized as well as their functional properties and antioxidant activities were investigated. The extraction yield, on a wet weight basis, was 7.35% and 7.46% for WSPP and WSPC, respectively. The most abundant amino acid in both proteins was glutamic acid, followed by aspartic, alanine, and leucine, in that order. The electrophoretic study revealed that proteins with MW of 29, 42, 50, 69, and 146 kDa were the major protein components in WSPP and WSPC. FTIR analysis showed that those proteins remained their ...


Mtorc1 Plays An Important Role In Osteoblastic Regulation Of B-Lymphopoiesis, Sally K. Martin, Stephen Fitter, Nadia El Khawanky, Randall H. Grose, Carl Walkley, Louise E. Purton, Markus A. Ruegg, Michael N. Hall, Stan Gronthos, Andrew C. W. Zannettino Jan 2018

Mtorc1 Plays An Important Role In Osteoblastic Regulation Of B-Lymphopoiesis, Sally K. Martin, Stephen Fitter, Nadia El Khawanky, Randall H. Grose, Carl Walkley, Louise E. Purton, Markus A. Ruegg, Michael N. Hall, Stan Gronthos, Andrew C. W. Zannettino

Faculty of Health Sciences Publications

Skeletal osteoblasts are important regulators of B-lymphopoiesis, serving as a rich source of factors such as CXCL12 and IL-7 which are crucial for B-cell development. Recent studies from our laboratory and others have shown that deletion of Rptor, a unique component of the mTORC1 nutrient-sensing complex, early in the osteoblast lineage development results in defective bone development in mice. In this study, we now demonstrate that mTORC1 signalling in pre-osteoblasts is required for normal B-lymphocyte development in mice. Targeted deletion of Rptor in osterix-expressing pre-osteoblasts (Rptorob−/−) leads to a significant reduction in the number of B-cells in the bone marrow ...


Possible Breakdown Of Dopamine Receptor Synergism In A Mouse Model Of Huntington's Disease, Samantha F. Kennedy Dec 2017

Possible Breakdown Of Dopamine Receptor Synergism In A Mouse Model Of Huntington's Disease, Samantha F. Kennedy

University of New Orleans Theses and Dissertations

The model of basal ganglia function proposed by Albin, Young and Penney (1989) describes two anatomically independent motor pathways, the direct and indirect. However, under normal conditions striatal dopamine (DA) is required for the expression of motor behavior, and DAergic control of the two pathways (via D1 and D2 receptors, respectively) is dependent on co-activation. We tested for a possible breakdown of D1/D2 synergism using transgenic R6/1 mice bearing the human huntingtin allele (Htt). Motor stereotypy, observed prior to the onset of HD-related symptoms, was rated on a 5-point scale following activation of: A) D1 receptors alone, B ...