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Articles 1 - 30 of 45
Full-Text Articles in Plant Pathology
Tal Effector-Nucleotide Targeter (Tale-Nt) 2.0: Tools For Tal Effector Design And Target Prediction, Erin L. Doyle, Nicholas J. Booher, Daniel S. Standage, Daniel F. Voytas, Volker P. Brendel, John K. Vandyk, Adam J. Bogdanove
Tal Effector-Nucleotide Targeter (Tale-Nt) 2.0: Tools For Tal Effector Design And Target Prediction, Erin L. Doyle, Nicholas J. Booher, Daniel S. Standage, Daniel F. Voytas, Volker P. Brendel, John K. Vandyk, Adam J. Bogdanove
Nicholas J. Booher
Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant pathogenic bacteria to manipulate host gene expression. Repeats are polymorphic and individually specify single nucleotides in the DNA target, with some degeneracy. A TAL effector-nucleotide binding code that links repeat type to specified nucleotide enables prediction of genomic binding sites for TAL effectors and customization of TAL effectors for use in DNA targeting, in particular as custom transcription factors for engineered gene regulation and as site-specific nucleases for genome editing. We have developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 (TALE-NT 2.0;https://boglab.plp.iastate.edu/) that enables design …
Interchromosomal Transfer Of Immune Regulation During Infection Of Barley With The Powdery Mildew Pathogen, Priyanka Surana, Ruo Xu, Gregory Fuerst, Antony V. E. Chapman, Dan Nettleton, Roger P. Wise
Interchromosomal Transfer Of Immune Regulation During Infection Of Barley With The Powdery Mildew Pathogen, Priyanka Surana, Ruo Xu, Gregory Fuerst, Antony V. E. Chapman, Dan Nettleton, Roger P. Wise
Dan Nettleton
Powdery mildew pathogens colonize over 9500 plant species, causing critical yield loss. The Ascomycete fungus, Blumeria graminis f. sp. hordei (Bgh), causes powdery mildew disease in barley (Hordeum vulgare L.). Successful infection begins with penetration of host epidermal cells, culminating in haustorial feeding structures, facilitating delivery of fungal effectors to the plant and exchange of nutrients from host to pathogen. We used expression Quantitative Trait Locus (eQTL) analysis to dissect the temporal control of immunity-associated gene expression in a doubled haploid barley population challenged with Bgh. Two highly significant regions possessing trans eQTL were identified near …
Parallel Genome-Wide Expression Profiling Of Host And Pathogen During Soybean Cyst Nematode Infection Of Soybean, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, Melissa G. Mitchum
Parallel Genome-Wide Expression Profiling Of Host And Pathogen During Soybean Cyst Nematode Infection Of Soybean, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, Melissa G. Mitchum
Dan Nettleton
Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential …
Sequence Mining And Transcript Profiling To Explore Cyst Nematode Parasitism, Axel A. Elling, Makedonka Mitreva, Xiaowu Gai, John Martin, Justin Recknor, Eric L. Davis, Richard S. Hussey, Dan Nettleton, James P. Mccarter, Thomas J. Baum
Sequence Mining And Transcript Profiling To Explore Cyst Nematode Parasitism, Axel A. Elling, Makedonka Mitreva, Xiaowu Gai, John Martin, Justin Recknor, Eric L. Davis, Richard S. Hussey, Dan Nettleton, James P. Mccarter, Thomas J. Baum
Dan Nettleton
Background: Cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins. These proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants.
Results: We present here the expression patterns of all previously described parasitism genes of the soybean cyst nematode, Heterodera glycines, in all major life stages except the adult male. These insights were gained by analyzing our gene expression dataset from experiments using the Affymetrix Soybean …
Flor Revisited (Again): Eqtl And Mutational Analysis Of Nb-Lrr Mediated Immunity To Powdery Mildew In Barley, Roger P. Wise, Priyanka Surana, Gregory S. Fuerst, Ruo Xu, Divya Mistry, Julie A. Dickerson, Dan Nettleton Dr.
Flor Revisited (Again): Eqtl And Mutational Analysis Of Nb-Lrr Mediated Immunity To Powdery Mildew In Barley, Roger P. Wise, Priyanka Surana, Gregory S. Fuerst, Ruo Xu, Divya Mistry, Julie A. Dickerson, Dan Nettleton Dr.
Dan Nettleton
Genes encoding early signaling events in pathogen defense often are identified only by their phenotype. Such genes involved in barley-powdery mildew interactions include Mla, specifying race-specific resistance; Rar1 (Required for Mla12-specified resistance1), and Rom1 (Restoration of Mla-specified resistance1). The HSP90-SGT1-RAR1 complex appears to function as chaperone in MLA-specified resistance, however, much remains to be discovered regarding the precise signaling underlying plant immunity. Genetic analyses of fast-neutron mutants derived from CI 16151 (Mla6) uncovered a novel locus, designated Rar3 (Required for Mla6-specified resistance3). Rar3 segregates independent of Mla6 and Rar1, and rar3 mutants are susceptible to Blumeria graminis f. sp. hordei …
Biphasic Gene Expression Changes Elicited By Phakopsora Pachyrhizi In Soybean Correlate With Fungal Penetration And Haustoria Formation, Katherine T. Schneider, Martijn Van De Mortel, Timothy J. Bancroft, Edward J. Braun, Dan Nettleton, Rex T. Nelson, Reid D. Frederick, Thomas J. Baum, Michelle Graham, Steven A. Whitham
Biphasic Gene Expression Changes Elicited By Phakopsora Pachyrhizi In Soybean Correlate With Fungal Penetration And Haustoria Formation, Katherine T. Schneider, Martijn Van De Mortel, Timothy J. Bancroft, Edward J. Braun, Dan Nettleton, Rex T. Nelson, Reid D. Frederick, Thomas J. Baum, Michelle Graham, Steven A. Whitham
Dan Nettleton
Inoculation of soybean (Glycine max) plants with Phakopsora pachyrhizi, the causal organism of Asian soybean rust, elicits a biphasic response characterized by a burst of differential gene expression in the first 12 h. A quiescent period occurs from 24 to 48 h after inoculation, in which P. pachyrhizi continues to develop but does not elicit strong host responses, followed by a second phase of intense gene expression. To correlate soybean responses with P. pachyrhizi growth and development, we inoculated the soybean cultivar Ankur (accession PI462312), which carries the Rpp3 resistance gene, with avirulent and virulent isolates …
Distinct Biphasic Mrna Changes In Response To Asian Soybean Rust Infection, Martijn Van De Mortel, Justin C. Recknor, Michelle Graham, Dan Nettleton, Jaime D. Dittman, Rex T. Nelson, Claudia V. Godoy, Ricardo V. Abdelnoor, Alvaro M.R. Almeida, Thomas J. Baum, Steven A. Whitham
Distinct Biphasic Mrna Changes In Response To Asian Soybean Rust Infection, Martijn Van De Mortel, Justin C. Recknor, Michelle Graham, Dan Nettleton, Jaime D. Dittman, Rex T. Nelson, Claudia V. Godoy, Ricardo V. Abdelnoor, Alvaro M.R. Almeida, Thomas J. Baum, Steven A. Whitham
Dan Nettleton
Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. …
Developmental Transcript Profiling Of Cyst Nematode Feeding Cells In Soybean Roots, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Tom Maier, Thomas J. Baum, Melissa G. Mitchum
Developmental Transcript Profiling Of Cyst Nematode Feeding Cells In Soybean Roots, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Tom Maier, Thomas J. Baum, Melissa G. Mitchum
Dan Nettleton
Cyst nematodes of the genus Heterodera are obligate, sedentary endoparasites that have developed highly evolved relationships with specific host plant species. Successful parasitism involves significant physiological and morphological changes to plant root cells for the formation of specialized feeding cells called syncytia. To better understand the molecular mechanisms that lead to the development of nematode feeding cells, transcript profiling was conducted on developing syncytia induced by the soybean cyst nematode Heterodera glycines in soybean roots by coupling laser capture microdissection with high-density oligonucleotide microarray analysis. This approach has identified pathways that may play intrinsic roles in syncytium induction, formation, and …
Rethinking Invasion Impacts Across Multiple Field Sites Using European Swallowwort (Vincetoxicum Rossicum) As A Model Invader, Grant L. Thompson, Terrence H. Bell, Jenny Kao-Kniffin
Rethinking Invasion Impacts Across Multiple Field Sites Using European Swallowwort (Vincetoxicum Rossicum) As A Model Invader, Grant L. Thompson, Terrence H. Bell, Jenny Kao-Kniffin
Grant Thompson
Surface-Soil Properties Of Alder Balds With Respect To Grassy And Rhododendron Balds On Roan Mountain, North Carolina—Tennessee, James T. Donaldson, Zachary C. Dinkins, Foster Levy, Arpita Nandi
Surface-Soil Properties Of Alder Balds With Respect To Grassy And Rhododendron Balds On Roan Mountain, North Carolina—Tennessee, James T. Donaldson, Zachary C. Dinkins, Foster Levy, Arpita Nandi
Foster Levy
We analyzed soils in Alder Bald, Grassy Bald, and Rhododendron Bald communities on Roan Mountain to infer the influence of vegetation on soil and to help guide management strategies. In all vegetation types, soils were acid (pH = 4–5) sandy loams. We found vegetation-associated differences for organic content, cation exchange capacity, acidity, two plant macronutrients (K, Mg), and three cations (Fe, Na, Zn). We predicted that nitrogen compounds would be highest in the Alder Bald because Alnus viridis ssp. crispa (Green Alder) can harbor nitrogen-fixing bacteria. Organic content was highest at the alder-bald sites, ammonium was similar among vegetation types, …
Pattern And Rate Of Decline Of A Population Of Carolina Hemlock (Tsuga Caroliniana Engelm.) In North Carolina, Foster Levy, Elaine S. Walker
Pattern And Rate Of Decline Of A Population Of Carolina Hemlock (Tsuga Caroliniana Engelm.) In North Carolina, Foster Levy, Elaine S. Walker
Foster Levy
We monitored a population of Carolina Hemlocks in northwestern North Carolina for four years to examine the rate and pattern of decline in response to infestation by Adelges tsugae (Hemlock Woolly Adelgid). Our yearly census of hemlock condition and severity of the adelgid infestation included trees of all sizes. We estimated declines in condition as the portions of the leaf canopy that were lost. Initially, infestation occurred throughout the population but was severe in only a small cluster of individuals. Within 1 year, the area of severe infestation increased in size to encompass 48% of the population. In another region …
Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson
Supplemental Data, Russell J. Ingram, Foster Levy, Cindy L. Barrett, James T. Donaldson
Foster Levy
No abstract provided.
Deidamia Inscriptum (Lettered Sphinx Moth) Caterpillars Feeding On Oxydendrum Arboreum (Sourwood) And Their Predation By Black Bears In Northeast Tennessee, Foster Levy, David L. Wagner, Elaine S. Walker
Deidamia Inscriptum (Lettered Sphinx Moth) Caterpillars Feeding On Oxydendrum Arboreum (Sourwood) And Their Predation By Black Bears In Northeast Tennessee, Foster Levy, David L. Wagner, Elaine S. Walker
Foster Levy
An outbreak of Deidamia inscriptum (Lettered Sphinx Moth) caterpillars was noted in northeast Tennessee where Oxydendrum arboreum (Sourwood) trees were defoliated. Nearly all published literature and online resources list only plants in the grape family (Vitaceae) as larval food plants. Food-plant preference trials using fresh leaves of 3 woody plant species showed that Deidamiacaterpillars from this region had a preference for Sourwood over Parthenocissus quinquefolia(Virginia Creeper), and rejected Acer rubrum (Red Maple), a non-host species. Ursus americanus(Black Bear) were feeding on the caterpillars as evidenced by bent and broken Sourwood saplings bearing claw marks and by abundant …
Applying Biodiversity And Ecosystem Function Theory To Turfgrass Management, Grant L. Thompson, Jenny Kao-Kniffin
Applying Biodiversity And Ecosystem Function Theory To Turfgrass Management, Grant L. Thompson, Jenny Kao-Kniffin
Grant Thompson
Corn Nitrogen Fertilization Requirement And Corn–Soybean Productivity With A Rye Cover Crop, Jose L. Pantoja, Krishna P. Woli, John E. Sawyer, Daniel W. Barker
Corn Nitrogen Fertilization Requirement And Corn–Soybean Productivity With A Rye Cover Crop, Jose L. Pantoja, Krishna P. Woli, John E. Sawyer, Daniel W. Barker
John E. Sawyer
Winter rye (Secale cereale L.) cover crop (RCC) has potential to reduce NO3–N loss from corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] fields. However, RCC effects on annual crop productivity and corn optimal N fertilization requirement are unclear. The objectives were to evaluate corn and soybean yield response to RCC and corn optimal N rate. Treatments were no-RCC and RCC with six fertilizer N rates (0–225 kg N ha-1) applied to corn in a no-till corn–soybean (CS) rotation at four Iowa sites in 2009 through 2011. The RCC biomass and …
Standardized Research Protocols Enable Transdisciplinary Research Of Climate Variation Impacts In Corn Production Systems, E. J. Kladivko, M. J. Helmers, L. J. Abendroth, D. Herzmann, R. Lal, M. J. Castellano, D. S. Mueller, J. E. Sawyer, R. P. Anex, R. W. Arritt, B. Basso, J. V. Bonta, L. C. Bowling, R. M. Cruse, N. R. Fausey, J. R. Frankenberger, P. W. Gassman, A. J. Gassmann, C. L. Kling, A. Kravchenko, J. G. Lauer, F. E. Miguez, E. D. Nafziger, N. Nkongolo, M. O'Neal, L. B. Owens, P. R. Owens, P. Scharf, M. J. Shipitalo, J. S. Strock, M. B. Villamil
Standardized Research Protocols Enable Transdisciplinary Research Of Climate Variation Impacts In Corn Production Systems, E. J. Kladivko, M. J. Helmers, L. J. Abendroth, D. Herzmann, R. Lal, M. J. Castellano, D. S. Mueller, J. E. Sawyer, R. P. Anex, R. W. Arritt, B. Basso, J. V. Bonta, L. C. Bowling, R. M. Cruse, N. R. Fausey, J. R. Frankenberger, P. W. Gassman, A. J. Gassmann, C. L. Kling, A. Kravchenko, J. G. Lauer, F. E. Miguez, E. D. Nafziger, N. Nkongolo, M. O'Neal, L. B. Owens, P. R. Owens, P. Scharf, M. J. Shipitalo, J. S. Strock, M. B. Villamil
John E. Sawyer
The important questions about agriculture, climate, and sustainability have become increasingly complex and require a coordinated, multifaceted approach for developing new knowledge and understanding. A multistate, transdisciplinary project was begun in 2011 to study the potential for both mitigation and adaptation of corn-based cropping systems to climate variations. The team is measuring the baseline as well as change of the system's carbon (C), nitrogen (N), and water footprints, crop productivity, and pest pressure in response to existing and novel production practices. Nine states and 11 institutions are participating in the project, necessitating a well thought out approach to coordinating field …
Soybean Top Dieback Shows Up In Iowa Again, Xiao-Bing Yang, John E. Sawyer
Soybean Top Dieback Shows Up In Iowa Again, Xiao-Bing Yang, John E. Sawyer
John E. Sawyer
Extension field agronomists report the occurrence of soybean top dieback in northeastern Iowa soybean fields this week. Soybean top dieback symptoms have shown up occasionally since it was first reported by Jim Fawcett and Jerry Long in Cedar County a few years ago. Last year it was also found in eastern and central Iowa.
Corn Following Corn In 2008, Mahdi Al-Kaisi, Roger W. Elmore, Antonio P. Mallarino, Palle Pedersen, Alison E. Robertson, John E. Sawyer, Jon J. Tollefson
Corn Following Corn In 2008, Mahdi Al-Kaisi, Roger W. Elmore, Antonio P. Mallarino, Palle Pedersen, Alison E. Robertson, John E. Sawyer, Jon J. Tollefson
John E. Sawyer
Corn following corn is in rough shape in areas across Iowa. Many wonder what is happening. The crop’s condition in general is not normal for this time of year. For example, last Sunday the USDA rated this year’s Iowa corn crop as 54 percent in ‘Good’ to ‘Excellent’ condition and 15 percent is ‘Poor’ or ‘Very Poor.’ Last year 72 percent was rated ‘Good’ to ‘Excellent’ and 5 percent was in ‘Poor’ or ‘Very Poor’ condition during the same week. Average plant height as of Sunday was 24 inches compared to 40 inches at end of the same week last …
Corn Field Guide, Lori Abendroth, Roger Elmore, Robert G. Hartzler, Clarke Mcgrath, Daren S. Mueller, Gary P. Munkvold, Richard Pope, Marlin E. Rice, Alison E. Robertson, John E. Sawyer, Kristine J. P. Schaefer, Jon James Tollefson, Gregory L. Tylka
Corn Field Guide, Lori Abendroth, Roger Elmore, Robert G. Hartzler, Clarke Mcgrath, Daren S. Mueller, Gary P. Munkvold, Richard Pope, Marlin E. Rice, Alison E. Robertson, John E. Sawyer, Kristine J. P. Schaefer, Jon James Tollefson, Gregory L. Tylka
John E. Sawyer
A reference for identifying diseases, insect pests, and disorders of corn.
Diversity Enhances Npp, N Retention, And Soil Microbial Diversity In Experimental Urban Grassland Assemblages, Grant L. Thompson, Jenny Kao-Kniffin
Diversity Enhances Npp, N Retention, And Soil Microbial Diversity In Experimental Urban Grassland Assemblages, Grant L. Thompson, Jenny Kao-Kniffin
Grant Thompson
A Position Paper On The Electronic Publication Of Nematode Taxonomic Manuscripts, Eyualem Abebe, James G. Baldwin, Byron Adams, Duane Hope, Scott Gardner, Robin Huettel, Peter Mullin, Thomas O. Powers, Jyotsna Sharma, Weimin Ye, William K. Thomas
A Position Paper On The Electronic Publication Of Nematode Taxonomic Manuscripts, Eyualem Abebe, James G. Baldwin, Byron Adams, Duane Hope, Scott Gardner, Robin Huettel, Peter Mullin, Thomas O. Powers, Jyotsna Sharma, Weimin Ye, William K. Thomas
Byron Adams
Several nematode species have now attained ‘model organism’ status, yet there remain many niches in basic biological inquiry for which nematodes would be ideal model systems of study. However, furthering the model system approach is hindered by lack of information on nematode biodiversity. The shortage of taxonomic resources to inventory and characterize biodiversity hinders research programs in invasion biology, ecosystem functioning, conservation biology, and many others. The disproportion between numbers of species to be described and numbers of available taxonomic specialists is greater for Nematoda than for any other metazoan phylum. A partial solution to the taxonomic impediment is the …
Novel Application Of Phastsystem Polyacrylamide Gel Electrophoresis Using Restriction Fragment Length Polymorphism ± Internal Transcribed Spacer Patterns Of Individuals For Molecular Identification Of Entomopathogenic Nematodes, Horolma Pamjav, Dimitra Triga, Zsuzsanna Buzµs, Tibor Vellai, Attila Lucskai, Byron Adams, Alexander P. Reid, Ann Burnell, Christine Griffin, Itamar Glazer, Michael G. Klein, Andras Fodor
Novel Application Of Phastsystem Polyacrylamide Gel Electrophoresis Using Restriction Fragment Length Polymorphism ± Internal Transcribed Spacer Patterns Of Individuals For Molecular Identification Of Entomopathogenic Nematodes, Horolma Pamjav, Dimitra Triga, Zsuzsanna Buzµs, Tibor Vellai, Attila Lucskai, Byron Adams, Alexander P. Reid, Ann Burnell, Christine Griffin, Itamar Glazer, Michael G. Klein, Andras Fodor
Byron Adams
A relatively rapid and economic way of identifying and assigning nematodes to taxons, which had already been determined either by comparative sequence analysis of nuclear rDNA internal transcribed spacer (ITS) region or by other methods of molecular or conventional taxonomy, is provided. Molecular identification of entomopathogenic nematodes (EPN) can be upgraded by basing it on PhastSystem polyacrylamide gel electrophoresis (PAGE) analysis of restriction fragment length polymorphism (RFLP) patterns of polymerase chain reaction (PCR)-amplified DNA derived from single nematodes of Steinernema or Heterorhabditis spp. Although analysis from single worms has previously been made on agarose gel, the resolution on PhastSystem PAGE …
Tal Effector-Nucleotide Targeter (Tale-Nt) 2.0: Tools For Tal Effector Design And Target Prediction, Erin L. Doyle, Nicholas J. Booher, Daniel S. Standage, Daniel F. Voytas, Volker P. Brendel, John K. Vandyk, Adam J. Bogdanove
Tal Effector-Nucleotide Targeter (Tale-Nt) 2.0: Tools For Tal Effector Design And Target Prediction, Erin L. Doyle, Nicholas J. Booher, Daniel S. Standage, Daniel F. Voytas, Volker P. Brendel, John K. Vandyk, Adam J. Bogdanove
John K. VanDyk
Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant pathogenic bacteria to manipulate host gene expression. Repeats are polymorphic and individually specify single nucleotides in the DNA target, with some degeneracy. A TAL effector-nucleotide binding code that links repeat type to specified nucleotide enables prediction of genomic binding sites for TAL effectors and customization of TAL effectors for use in DNA targeting, in particular as custom transcription factors for engineered gene regulation and as site-specific nucleases for genome editing. We have developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 (TALE-NT 2.0;https://boglab.plp.iastate.edu/) that enables design of custom …
Wheat Streak Mosaic Virus Infects Systemically Despite Extensive Coat Protein Deletions: Identification Of Virion Assembly And Cell-To-Cell Movement Determinants, Satyanarayana Tatineni, Frank A. Kovacs, Roy C. French
Wheat Streak Mosaic Virus Infects Systemically Despite Extensive Coat Protein Deletions: Identification Of Virion Assembly And Cell-To-Cell Movement Determinants, Satyanarayana Tatineni, Frank A. Kovacs, Roy C. French
Satyanarayana Tatineni
Viral coat proteins function in virion assembly and virus biology in a tightly coordinated manner with a role for virtually every amino acid. In this study, we demonstrated that the coat protein (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus; family Potyviridae) is unusually tolerant of extensive deletions with continued virion assembly and/or systemic infection. A series of deletion and point mutations were created in the CP cistron of wild-type and/or GFP-tagged WSMV and examined the effects of these mutations on cell-to-cell and systemic transport and virion assembly of WSMV. Mutants with overlapping deletions comprising N-terminal amino acids 6 …
Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a, Gerhard Thiel, Anna Moroni, David Dunigan, James L. Van Etten
Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a, Gerhard Thiel, Anna Moroni, David Dunigan, James L. Van Etten
David D Dunigan Ph. D.
Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms …
Dna Viruses: The Really Big Ones (Giruses), James L. Van Etten, Leslie C. Lane, David Dunigan
Dna Viruses: The Really Big Ones (Giruses), James L. Van Etten, Leslie C. Lane, David Dunigan
David D Dunigan Ph. D.
Viruses with genomes greater than 300 kb and up to 1200 kb are being discovered with increasing frequency. These large viruses (often called giruses) can encode up to 900 proteins and also many tRNAs. Consequently, these viruses have more protein-encoding genes than many bacteria, and the concept of small particle/small genome that once defined viruses is no longer valid. Giruses infect bacteria and animals although most of the recently discovered ones infect protists. Thus, genome gigantism is not restricted to a specific host or phylogenetic clade. To date, most of the giruses are associated with aqueous environments. Many of these …
Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten
Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten
David D Dunigan Ph. D.
Taxonomy: Chlorella viruses are assigned to the family Phycodnaviridae, genus Chlorovirus, and are divided into three species: Chlorella NC64A viruses, Chlorella Pbi viruses and Hydra viridis Chlorella viruses. Chlorella viruses are large, icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorella-like green algae. The type member is Paramecium bursaria chlorella virus 1 (PBCV-1).
Physical properties: Chlorella virus particles are large (molecular weight ~1 × 109 Da) and complex. The virion of PBCV-1 contains more than 100 different proteins; the major capsid protein, Vp54, comprises ~40% of the virus protein. Cryoelectron microscopy and three-dimensional image reconstruction of PBCV-1 virions …
'Earlibrite' Strawberry, C. K. Chandler, D. E. Legard, David Dunigan, T. E. Crocker, C. A. Sims
'Earlibrite' Strawberry, C. K. Chandler, D. E. Legard, David Dunigan, T. E. Crocker, C. A. Sims
David D Dunigan Ph. D.
There is a need in west central Florida and other winter strawberry (Fragaria xananassa Duchesne) production areas for an early ripening cultivar to replace or be an alternative to 'Sweetcharlie' (Chandleretal., 1997a). 'Sweet Charlie' has benefitted the Florida strawberry industry through its relatively high production of fruit early in the season, when market prices are generally high. But the average fruit size is small, and the texture of its fruit is often soft, making shipment and shelf life of 'Sweet Charlie' problematic. 'Earlibrite' strawberry has produced high early-season (December through February) yields of large, flavorful fruit at the Univ. of …
Phycodnaviruses: A Peek At Genetic Diversity, David Dunigan, Lisa Fitzgerald, James L. Van Etten
Phycodnaviruses: A Peek At Genetic Diversity, David Dunigan, Lisa Fitzgerald, James L. Van Etten
David D Dunigan Ph. D.
The family Phycodnaviridae encompasses a diverse collection of large icosahedral, dsDNA viruses infecting algae. These viruses have genomes ranging from 160 to 560 kb. The family consists of six genera based initially on host range and supported by sequence comparisons. The family is monophyletic with branches for each genus, but the phycodnaviruses have evolutionary roots that connect with several other families of large DNA viruses, referred to as the nucleocytoplasmic large DNA viruses (NCLDV). The genomes of members in three genera in the Phycodnaviridae have recently been sequenced and the purpose of this manuscript is to summarize these data. The …
Microarray Analysis Of Paramecium Bursaria Chlorella Virus 1 Transcription, Giane M. Yanai-Balser, Garry A. Duncan, James D. Eudy, Dong Wang, Xiao Li, Irina V. Agarkova, David Dunigan, James L. Van Etten
Microarray Analysis Of Paramecium Bursaria Chlorella Virus 1 Transcription, Giane M. Yanai-Balser, Garry A. Duncan, James D. Eudy, Dong Wang, Xiao Li, Irina V. Agarkova, David Dunigan, James L. Van Etten
David D Dunigan Ph. D.
Paramecium bursaria chlorella virus 1 (PBCV-1), a member of the family Phycodnaviridae, is a large double-stranded DNA, plaque-forming virus that infects the unicellular green alga Chlorella sp. strain NC64A. The 330-kb PBCV-1 genome is predicted to encode 365 proteins and 11 tRNAs. To monitor global transcription during PBCV-1 replication, a microarray containing 50-mer probes to the PBCV-1 365 protein-encoding genes (CDSs) was constructed. Competitive hybridization experiments were conducted by using cDNAs from poly(A)- containing RNAs obtained from cells at seven time points after virus infection. The results led to the following conclusions: (i) the PBCV-1 replication cycle is temporally programmed …