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Full-Text Articles in Agronomy and Crop Sciences
Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson, Fiona Goggin
Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson, Fiona Goggin
Inquiry: The University of Arkansas Undergraduate Research Journal
Plant elicitor peptides (Peps) – endogenous chains of amino acids involved in natural plant defense – have been shown to decrease damage from herbivores and pathogens by inducing an immune response, increasing the emission of volatile organic compounds (VOCS), transcripts, and metabolites. Exogenous treatment of soybean seeds with plant elicitor peptide GmPep3 has been shown to induce these broad-spectrum defenses and offers a new method for increasing crop yield. However, the effects of GmPep3 on indicators of soybean health – root characteristics, growth stages, etc. – have not been fully realized. Using the root-phenotyping platform RhizoVision Explorer, several root traits …
Use Of Calcium Hypochlorite As A Sanitizer For Seeds Used For Sprouting: Task #2; Impact: Improved Alfalfa Decontamination Technologies, Emily Damron, Carrie Klein, Melissa Leach, Jordan Mourot, Tom Murphy, Amy Seamans, Ryan Wilson
Use Of Calcium Hypochlorite As A Sanitizer For Seeds Used For Sprouting: Task #2; Impact: Improved Alfalfa Decontamination Technologies, Emily Damron, Carrie Klein, Melissa Leach, Jordan Mourot, Tom Murphy, Amy Seamans, Ryan Wilson
Inquiry: The University of Arkansas Undergraduate Research Journal
Consumption of raw or lightly cooked alfalfa sprouts has been a concern of the U.S. Food and Drug Administration (FDA) in recent years due to connections between sprouts and foodborne illnesses. Researchers have identified, contaminated seeds as the primary source of alfalfa sprouts contamination. Contamination of alfalfa seeds can originate in the field, harvesting, storing, or sprouting. Two pathogens of particular concern on alfalfa seeds are Escherichia coli 0157:H7 (E. coli) and Salmonella. These pathogens are capable of producing biofilms that provide protection for individual cells and allow for survival in otherwise hostile environments, including some disinfectant washes. Other factors …
Propagation Of Thornless Blackberries Utilizing Adventitious Shoots From Root Cuttings, Ellen Thompson
Propagation Of Thornless Blackberries Utilizing Adventitious Shoots From Root Cuttings, Ellen Thompson
Inquiry: The University of Arkansas Undergraduate Research Journal
Studies were conducted in early 2003 to determine the effect of root source and length on yield of adventitious shoots from root cuttings and on subsequent plant yield for University of Arkansas-developed thornless blackberries. In the first study, roots from 'Arapaho' and 'Apache' plants grown in an aboveground bed containing commercial potting soil were compared to field-grown roots. Bed-grown roots averaged 6.9 shoots per 15 cm root cutting while field grown roots averaged 3.4. 'Apache' produced more shoots/root cutting compared to Arapaho, (5.9 vs. 4.4 shoots/root cutting, respectively). In a comparison of 15- vs. 30-cm-long root cuttings of 'Apache', 'Arapaho', …
Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix
Chitosanase May Enhance Anti-Fungal Defense Responses In Transgenic Tobacco, Bill L. Hendrix
Inquiry: The University of Arkansas Undergraduate Research Journal
Fungicides are expensive, dangerous, and can be harmful to the environment, but they are often necessary for profitable farming operations. New technologies may soon allow farmers to replace these chemicals with genetically engineered plants producing antifungal enzymes that degrade fungal cell walls. To explore this option, a Paenbacillus chitosanase gene was cloned, sequenced, and modified for plant expression. The modified gene was delivered to tobacco (Nicotiana tabacum L cv. Xanthine) leaf disks via Agrobacterium tumenfaciens-mediated transformation. The putative GMOs were tested for transgene integration, transcription, and translation. Confirmed transformants were then screened for enhanced responses to a Rhizoctonia solani cell …
Characterization Of Wound-Inducible Genes Encoding Enzymes For Terpenoid Biosynthesis In Medicago Truncatula, Mandy M. Cox
Characterization Of Wound-Inducible Genes Encoding Enzymes For Terpenoid Biosynthesis In Medicago Truncatula, Mandy M. Cox
Inquiry: The University of Arkansas Undergraduate Research Journal
In addition to having numerous applications and pharmaceuticals, terpenoids are an important class of defensive compounds that can accumulate in plants after pathogen infection or injury by insects. Sequences of DNA encoding putative terpene synthases and an oxidosqualene synthase, isolated from insect-damaged Medicago truncatula leaves, were selected from an expressed sequence tag (EST) data base. The cDNA clones were used as radio-labeled probes to analyze gene expression in leaves treated with factors known to trigger a defense response in plants. Transcript levels for all of the genes examined increased in response to artificial wounding, insect herbivory, and methyl jasmonate (meJA) …
Response Of Blackberry Cultivars To Nematode Transmission Of Tobacco Ringspot Virus, Alisha Sanny
Response Of Blackberry Cultivars To Nematode Transmission Of Tobacco Ringspot Virus, Alisha Sanny
Inquiry: The University of Arkansas Undergraduate Research Journal
A study was conducted on eight cultivars of blackberry ('Apache', 'Arapaho', 'Chester', 'Chickasaw', 'Kiowa', 'Navaho', 'Shawnee', and 'Triple Crown'), of which four plants of each were previously determined in the fall of 2001 to have root, but not leaf, infection with Tobacco ringspot virus (TRSV). The objective of our study was to determine virus effects on plant vigor, and the spread of virus infection in the plants. Eight plants of each cultivar, four infected and four free of infection, were grown in pots on a gravel pad for the 2002 growing season, and samples of primocane and floricane leaves were …
Evaluation Of Chilling Requirements For Six Arkansas Blackberry Cultivars Utilizing Stem Cuttings, Dayanee Yazzetti
Evaluation Of Chilling Requirements For Six Arkansas Blackberry Cultivars Utilizing Stem Cuttings, Dayanee Yazzetti
Inquiry: The University of Arkansas Undergraduate Research Journal
Woody perennial plants including blackberries (Rubus subgenus Rubus Watson) require certain amounts of chilling or rest hours below 7T during the dormant season for successful bud break the following year. Blackberry cultivars developed in Arkansas are being grown in various climates worldwide, and all cultivars need chilling requirement estimates for accurate recommendations of adaptation. Determining chilling requirements using stem cuttings collected from field-grown plants rather than whole plants is a desirable system. We conducted a study to evaluate both artificial- and field-chilling of six cultivars. For the artificial-chilling study, 12-node stem cuttings were collected 2 days after the first killing …