Physiology Commons^™

A Bioinformatics Approach To Understanding The Pathogenesis Of Ectopic Spine Calcification, Fang Chi Wang

Electronic Thesis and Dissertation Repository

This study used mice lacking equilibrative nucleoside transporter 1 (ENT1^-/-) as a preclinical model to study pathogenesis of ectopic spine mineralization in diffuse idiopathic skeletal hyperostosis (DISH). We hypothesized that mineralization of the annulus fibrosus (AF), was driven by dysregulation of cellular processes and pathways associated with apoptosis, S100A9 proteins, the PI3K-Akt pathway, and lipid metabolism. Target pathways and processes were assessed using in situ localization and quantitative analyses. Metabolomic analysis of AF and plasma data was conducted to identify altered metabolites. Results demonstrate increased caspase-3 activity in the AF of ENT1^-/- mice at both timepoints …

Short-Term Hiit Impacts Hdl Function Differently In Lean, Obese, And Diabetic Subjects, Lin Zhu, Julia An, Thao Luu, Sara M. Reyna, Puntip Tantiwong, Apiradee Sriwijitkamol, Nicolas Musi, John M. Stafford

School of Medicine Publications and Presentations

Introduction: High density lipoproteins (HDL) exert cardiovascular protection in part through their antioxidant capacity and cholesterol efflux function. Effects of exercise training on HDL function are yet to be well established, while impact on triacylglycerol (TG)-lowering has been often reported. We previously showed that a short-term high-intensity interval training (HIIT) program improves insulin sensitivity but does not inhibit inflammatory pathways in immune cells in insulin-resistant subjects. The purpose of this study is to evaluate HDL function along with changes of lipoproteins after the short-term HIIT program in lean, obese nondiabetic, and obese type 2 diabetic (T2DM) subjects.

Methods: All individuals …

Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …

The Development, Implementation, And Value Of The Athletic Training Milestones For Assessing Progressive Clinical Behaviors, Hollie Walusz, Eric Sauers, Mark Laursen, Forrest Pecha

Clinical Practice in Athletic Training

No abstract provided.

The Value Of Using Validated Competency-Based Assessments Across The Career Span Of An Athletic Trainer, Matthew Drescher, Lindsey Eberman

Clinical Practice in Athletic Training

No abstract provided.

Perceptions And Use Of The Athletic Training Milestones In Education: A Report From The Aate Research Network, Cailee E. Welch Bacon, Barton E. Anderson, Julie M. Cavallario, Bonnie L. Van Lunen, Lindsey E. Eberman

Clinical Practice in Athletic Training

The Athletic Training Milestones (AT Milestones) were developed as an evaluation tool to capture the breadth and depth of athletic training knowledge, skills, and behavior. However, it is unclear whether athletic training programs are implementing this tool or how they are implementing it to gauge the clinical progression of students or residents in athletic training. We aimed to explore the perceptions and use of the AT Milestones among educators in athletic training programs. We used a cross-sectional, web- based survey with open-ended questions to collect data from athletic training programs on if and how they implement the AT Milestones at …

Standardized Patient Evaluation Tool: A Valid Measure Of The Core Competencies, Lindsey E. Eberman, Jessica Edler Nye

Clinical Practice in Athletic Training

No abstract provided.

Persistent Interruption In Parvalbuminpositive Inhibitory Interneurons: Biophysical And Mathematical Mechanisms, Carol M. Upchurch, Christopher J. Knowlton, Simon Chamberland, Carmen C. Canavier

School of Graduate Studies Faculty Publications

Persistent activity in excitatory pyramidal cells (PYRs) is a putative mechanism for maintaining memory traces during working memory. We have recently demonstrated persistent interruption of firing in fastspiking parvalbumin-expressing interneurons (PV-INs), a phenomenon that could serve as a substrate for persistent activity in PYRs through disinhibition lasting hundreds of milliseconds. Here, we find that hippocampal CA1 PV-INs exhibit type 2 excitability, like striatal and neocortical PV-INs. Modeling and mathematical analysis showed that the slowly inactivating potassium current KV1 contributes to type 2 excitability, enables the multiple firing regimes observed experimentally in PV-INs, and provides a mechanism for robust persistent interruption …

Measuring Activity Demands On Fatigue In Individuals With Parkinson's Disease, Laura R. Mccray, Kara M. Lee

Graduate Student Scholarship

The functional presentation of those with Parkinson's disease (PD) are complex, varying depending on cognition, physiological deficits, and support network. This study assesses the perceptions of fatigue across individuals with Parkinson's disease with different clinical presentations to guide clinicians in their selection of evidence-based interventions to facilitate greater functional outcomes. A mixed-methods design was used to collect data on the mental and physiological perceptions of fatigue, utilizing surveys and physiological measures such as heart rate. Participants were recruited from a community group targeting those with PD with inclusion criteria being a diagnosis of PD. Results for both heart rate and …

Effects Of Exercise Or Metformin On Myokine Concentrations In Patients With Breast And Colorectal Cancer: A Phase Ii Multi-Centre Factorial Randomized Trial, Justin C. Brown, Guillaume Spielmann, Shengping Yang, Stephanie L.E. Compton, Lee W. Jones, Melinda L. Irwin, Jennifer A. Ligibel, Jeffrey A. Meyerhardt

School of Medicine Faculty Publications

Background: Physical activity and metformin pharmacotherapy are associated with improved clinical outcomes in breast and colorectal cancer survivors. Myokines are cytokines secreted from skeletal muscle that may mediate these associations. Methods: This hypothesis-generating analysis used biospecimens collected from a multi-centre 2 × 2 factorial randomized design of 116 patients with stage I–III breast and colorectal cancer who were randomized to 12 weeks of (1) aerobic exercise (moderate intensity titrated to 220 min/week); (2) metformin (850 mg daily for 2 weeks and then titrated to 850 mg twice per day); (3) aerobic exercise and metformin; or (4) control. Fourteen myokines were …

Photobiomodulation Of Bovine Oocytes During Maturation Increases Atp Content And Enhances Subsequent Embryonic Development, Kendall Richey

All Theses

Bovine in-vitro production(IVP) of embryos is a growing field for producing offspring with desirable genetics in the cattle industries. While IVP is effective, oocyte cytoplasmic maturation is compromised, and bovine oocytes matured in-vitro have reduced metabolic activity than those matured in-vivo. Mitochondria are the central unit of oocyte metabolism, producing ATP through OXPHOS. Photobiomodulation is a light treatment reported to improve metabolic activity. In five experiments, we measured the effects of photobiomodulation treatment with red LED at 16-(L-16) and 20 h(L-20) of bovine oocyte in-vitro maturation on subsequent embryonic development, mitochondrial activity and nuclear progression. Bovine COCs were aspirated …

Effects Of Blood Flow Restriction During Acute Multi-Joint Eccentric Exercise On Muscle Recovery, Cody Fisher

All Graduate Reports and Creative Projects, Fall 2023 to Present

Blood flow restriction (BFR) exercise has become an increasingly common modality due to evidence that it may yield positive muscle-related effects from low-intensity exercise. However, eccentric-only exercise in a multi-joint format has not yet been investigated in regard to the BFR influence on muscle recovery characteristics. The purpose of this study was to assess muscle recovery responses via isometric peak force (PF), soreness (i.e., VAS, PPT, PPD), and functional power (i.e., SLHD) following a 5-minute low-load multi-joint eccentric exercise bout with or without blood flow restriction. Thirty participants were randomly assigned into either an eccentric-only (CON) or an eccentric BFR …

Investigating The Modulation Of Metastasis By Dax-1 In Adrenal Carcinoma Cells, Aarya Mishra

Undergraduate Honors Theses

The nuclear hormone receptor (NHR), DAX-1 (dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1), is important in adrenal and gonadal development as well as steroidogenesis. It is encoded by the NR0B1 gene and functions mainly as a transcriptional repressor. Classified as an orphan receptor within the NHR superfamily, DAX-1 has been shown to inhibit other NHRs including estrogen receptor, androgen receptor and steroidogenic factor 1. DAX-1 is found to be underexpressed in breast and prostate cancers and, specifically in prostate cancer, is believed to be a transcriptional repressor of genes that are involved in epithelial-mesenchymal transition …

Artificial Insemination And Fetal Programming In Beef Production Systems, Dempster Christenson

Department of Animal Science: Dissertations, Theses, and Student Research

Pregnancy to artificial insemination and late gestation fetal programming of the next calf crop are central to genetic development, reproductive longevity, calf crop productivity, and efficiency of cow/calf ranching. The purposes of this research are to find methods to improve pregnancy rate to artificial insemination, the growth and reproductive health of pregnant heifers, and the productivity of their offspring. In the first of four studies we found that extending the period of progesterone administration in an estrus synchronization protocol did not significantly hasten estrus response or increase pregnancy rate to artificial insemination, but timing of estrus within the melengestrol acetate …

The Role Of B Cell Activation State And Sex In Aryl Hydrocarbon Receptor Mediated Induction Of Chemokine Receptor 9 And Alpha4beta7 Expression In Vitro, Logan Bauerle

Master's Theses

Defense of mucosal tissues from microbial infection and allergy is reliant on continual production of antibodies. The aryl hydrocarbon receptor (AhR) is known to regulate B cell development and is associated with suppression of systemic humoral immunity. Recent attention has been paid to the role of the AhR in altering expression of cell adhesion molecules (CAMs). B cells express CAMs and chemokine receptors to migrate around the body for localized secretion of antibodies. AhR agonists promote B cell migration to the small intestine through upregulation of chemokine receptor 9 (CCR9) and integrin α₄β₇. Both the AhR …

Sarcopenia And The Importance Of Resistance Training And Protein-Rich Diets For Prevention Of Muscle Loss In Older Adults: A Literature Review And Informational Pamphlet, Madison Steele

Honors Theses

The growing prevalence of sarcopenia among older adults as well as youths has prompted further research aimed at developing effective preventative measures and treatment plans. Sarcopenia is often thought to occur solely due to aging, but several other factors contribute to progressive muscle loss. Previous research studies have found an effect of physical activity and dietary protein levels on the risk of developing sarcopenia as well as its onset age. This study aims to review existing knowledge in the field to compile an extensive list of sarcopenia causes and methods of prevention. The main causes identified in this review are …

Affective Temperaments And Personality Traits In Couple Well-Being, Carmela Mento, Chiara La Barbiera, Maria Catena Silvestri, Maria Rosaria Anna Muscatello, Clemente Cedro, Antonio Bruno, Gianluca Pandolfo, Fiammetta Iannuzzo, Clara Lombardo

Journal of Mind and Medical Sciences

Background. The objective of this study is to establish the link between affective temperament traits and maladaptive personality traits, to verify whether the potential presence of elements related to emotional, affective and dysfunctional relational functioning can affect the couple satisfaction, modifying the well-being or discomfort condition. Materials and Methods. A data collection questionnaire was developed to investigate the factors associated with dysfunctional emotional, affective, and relational modes of functioning. The sample consisted of 473 subjects. Data were collected including the TEMPS-A questionnaire, The Dirty Dozen Italian Assessment and the Relationship Assessment Scale (RAS). Results. The findings of this study showed …

Peak Aerobic Capacity And Dietary Composition Are Associated With The Bioenergetic Profile Of Platelets In Children, Duncan C. Troup

Scholars Day Conference

Atherosclerosis is a disease of the cardiovascular system that is characterized by the buildup of plaque inside arteries over several decades. These plaques have the potential to rupture, causing a clot to form in the vessel. This results in an acute cardiovascular event such as myocardial infarction or stroke. Atherosclerosis remains a significant public health challenge in developed countries, being the foundational cause of roughly 50% of deaths in westernized societies and a major contributor to health costs [13,14]. Understanding this devastating disease and developing preventative measures is a highly important area of research. While the impact of cardiovascular disease …

Gut Microbial Dysbiosis Differs In Two Distinct Cachectic Tumor-Bearing Models Consuming The Same Diet, Lauri O. Byerley, Brittany Lorenzen, Hsiao Man Chang, William G. Hartman, Michael J. Keenan, Ryan Page, Meng Luo, Scot E. Dowd, Christopher M. Taylor

School of Medicine Faculty Publications

The impact of cancer cachexia on the colonic microbiota is poorly characterized. This study assessed the effect of two cachectic-producing tumor types on the gut microbiota to determine if a similar dysbiosis could be found. In addition, it was determined if a diet containing an immunonutrient-rich food (walnuts) known to promote the growth of probiotic bacteria in the colon could alter the dysbiosis and slow cachexia. Male Fisher 344 rats were randomly assigned to a semi-purified diet with or without walnuts. Then, within each diet group, rats were further assigned randomly to a treatment group: tumor-bearing ad libitum fed (TB), …

Cortisol Production In Female Soccer Players, Katelyn Seagraves

ASPIRE 2024

Cortisol is released from the adrenal glands when there is an apparent stressor. Exercise, while beneficial, is seen as stress to the body and incites increased cortisol release. Cortisol is known to increase with exercise, and higher-intensity exercise tends to raise cortisol more compared to lower intensities. However, less is known about interval training and cortisol concentrations.

This review of literature dives into the background of cortisol: how it's produced, what systems it regulates, and differences in production based on gender, stressors, exercise intensity, and duration. This paper identifies some knowledge gaps and links training intensities in soccer to cortisol …

Occupational Engagement In Recreational Sports For The Deaf And Hard Of Hearing: An Employee Guidebook, Colleen Mcerlean, Mary Ann Smith, Christopher Ebner

Spring 2024 Virtual OTD Capstone Symposium

An employee guidebook to help increase occupational engagement within recreational sports for the Deaf and Hard of Hearing population in the Austin, TX area.

Effects Of Balance Training On Spinal Reflexive Excitability Modulation, Corticospinal Excitability, And Balance Performance In Individuals With Chronic Ankle Instability, Sunghoon Chung

Rehabilitation Sciences Theses & Dissertations

Chronic ankle instability (CAI) is a neurophysiologic deficit resulting in diverse sensorimotor impairments. Following acute ankle sprains, pain, mechanical instability, and joint deafferentation reduce sensory input to the central nervous system (CNS). In response, the CNS sends altered motor signals to lower extremity muscles. These CNS changes contribute to various neuromuscular impairments in CAI patients, the most common of which is reduced balance performance. Specifically, CAI patients struggle to modulate spinal reflex excitability of the soleus muscle when progressing from simpler to more complex balance tasks. This overreliance on spinal reflexes results in inconsistent activation of the ankle stabilizing muscle. …

Cortisol Production In Female Soccer Players, Katelyn Seagraves

Honors Projects

Cortisol is released from the adrenal glands when there is an apparent stressor. Exercise, while beneficial, is seen as stress to the body and incites increased cortisol release. Cortisol is known to increase with exercise, and higher-intensity exercise tends to raise cortisol more compared to lower intensities. However, less is known about interval training and cortisol concentrations.

This review of literature dives into the background of cortisol: how it's produced, what systems it regulates, and differences in production based on gender, stressors, exercise intensity, and duration. This paper identifies some knowledge gaps and links training intensities in soccer to cortisol …

Attitudes And Self-Efficacy Of Swimming Coaches Towards The Inclusion Of Swimmers With Autism Spectrum Disorder, Eleftheria Kasagianni, Dimitrios Kokaridas, Panagiotis Varsamis, Vasilis Tsimaras

International Journal of Aquatic Research and Education

The purpose of this study was to investigate the attitudes and self-efficacy of swimming coaches regarding the inclusion of swimmers with autism spectrum disorder (ASD). The sample consisted of 150 Greek swimming coaches with an average age of 29.58 years. Each participant completed the Swimming Coaches Attitudes towards Inclusion Questionnaire for perceptions assessment and the Biddle and Goudas (1997) self-efficacy questionnaire. The statistical analysis used SPSS 27 to calculate Cronbach’s alpha, Pearson product-moment correlations, independent t-tests, and ANOVA. The findings of the study showed that the swimming coaches expressed positive attitudes and a high percentage of perceived self-efficacy towards the …

‘You Have To Respect The Water’: Participant Experiences Of Appreciating And Managing The Risks Associated With Open Water Swimming – A Rapid Ethnographic Study, Mark A. Christie, David Elliott

International Journal of Aquatic Research and Education

Open water swimming (OWS) has rapidly grown in popularity, driven by the purported health benefits of cold-water immersion. A paucity of research remains specifically considering the notable risks inherent in OWS participation, and a lack of qualitative research on freshwater swimming experiences, and safety-related issues therein. This rapid ethnographic study, based at a dedicated OWS lake in the UK, conducted semi-structured interviews with OWS participants (n=17; female=11, male=6). Two core themes emerged: environmental issues impacting OWS experiences and behaviours; and knowledge and education of OWS which highlighted safe/unsafe practices, levels of education for managing risks, personal preparedness, swimming solo/with others, …

Barriers: Location, Functionality, And Method Of Access In Childhood Pool/Spa Submersion Incidents, United States, 2000-2017, Alison Miller, Kristina R. Anderson, William D. Ramos

International Journal of Aquatic Research and Education

Drowning is a leading cause of fatality among children in the United States, and residential pools/spas currently account for as much as 80% of these submersion incidents. This study reviewed narrative case reports obtained from the United States Consumer Product Safety Commission (CPSC) to understand barrier location, type, and functionality as methods of pool/spa access for childhood submersion incidents. Retroactive analysis of 1,523 fatal and non-fatal submersion incidents among children aged 13 years old and younger was conducted using the CPSC in depth investigation dataset from 2000-2017. Narrative descriptions were coded according to the attributes of barrier location, functionality, and …

Perceived And Real Water Competency And Drowning Risk Among Adults, Teresa Stanley, Kevin Moran, Dawn Garbett, Alan Ovens

International Journal of Aquatic Research and Education

Traditionally, public understanding of drowning survival has focused on swimming capacity, often measured by how far a person can swim. With respect to the high incidence of adult drowning in high-income countries, using the more inclusive concept of water competency may yield a more comprehensive explanatory framework for understanding the reasons behind these drownings. Any competency base required to survive a drowning situation is dynamic, complex, and multi-faceted. Furthermore, perceptions of risk and capacity to cope with that risk are likely to be pivotal to the avoidance of drowning. Adults’ perceived water competence was measured against their actual water competence …

A Research Framework To Improve Evidence-Based Practice Surrounding The Resuscitation Response To Drowning In Surf Lifesaving, Elissa Hooper, Aaron T. Scanlan, Shayne D. Baker, Samantha Fien

International Journal of Aquatic Research and Education

Surf lifesaving members are essential first responders who patrol public waterways, performing rescues and resuscitation to individuals in the community when needed. In this way, surf lifesaving members experience arduous physical requirements alongside extensive psychological stress during resuscitation. Surf Life Saving Queensland (SLSQ) represents the predominant, frontline lifesaving service in Queensland, Australia; however, little research has explored key aspects of drowning resuscitations involving this organisation, as well as in the broader lifesaving industry, limiting our current understanding on this vital topic. We sought to explore key aspects of drowning resuscitations within SLSQ and create a framework to generate evidence-based practice. …

Lay Rescuer Equipment Preferences And Efficacy During A Simulated Drowning Event, Alison M. Miller, William D. Ramos, Kristina R. Anderson, Jill Cuvala

International Journal of Aquatic Research and Education

During a drowning incident where a lifeguard is not present, a bystander – referred to as a lay rescuer - may put themselves in danger by attempting a rescue. When lay rescuers can avoid entering the water by using rescue equipment to help a drowning victim, it serves to not only help the person actively drowning, but also provides a layer of protection to a lay rescuer. This study sought to examine the following questions: (a) which pieces of rescue equipment were preferred by lay rescuers at pre-determined short and long distances, (b) do lay rescuers select appropriate rescue equipment …

Stress Hormone And Heart Rate Responses To Various Exercise Training Methods, Tomas Gomez Jr., Rebekah Schlatter, Megan Zamora, Marco Arriago, Sue Anne Chew, Murat Karabulut

Research Symposium

Background: Blood Flow Resistance (BFR) training has garnered attention for its ability to induce positive physiological adaptations with low-load resistance exercise. The present study aimed to examine the responses of catabolic hormones and heart rates (HR) to various BFR training protocols. This investigation seeks to provide insights into the stress levels induced by different protocols and identification behind the most effective protocol for optimal positive exercise-related adaptations.

Methods: Study population involved 10 healthy adult males (height: 175.0±5.0 cm, weight: 96.67±26.6 kg, age: 21.3±2.67 yr.) in a five-session investigation. Informed consent paperwork, pre-testing and anthropometric measurements served as session 1; sessions …