Virology Commons^™

Detection Of Neonatal Calf Diarrhea Virus (Ncdv) And Human Infant Reolike Diarrhea Virus, Martin Wyatt Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a diagnostic test and conduct a survey for the neonatal calf diarrhea virus (NCDV) and human infant reolike diarrhea virus. Two immunologic methods were developed in this investigation.

Immune electron microscopy (IEM) and the fluorescent viral precipitin test (FVPT) are the methods used to detect NCDV and the human virus. Both methods are based upon the principle that viral aggregates form when the virus is reacted with anti-NCDV antibody. Aggregates in the IEM method are negatively stained and observed with the use of an electron microscope. Fluorescein labeled antibody is used in …

Characterization Of A Bovine Parvovirus, Thomas J. Langpap

Electronic Theses and Dissertations

Bovine parvovirus has been shown to produce calf scours (40). Although exact figures are unavailable, a sizeable economic loss is suffered annually by South Dakotans as a result of this disease. Bovine parvovirus has been isolated in South Dakota (7). However, the extent of exposure to the virus is unknown. The object of this study is to characterize the South Dakota parvovirus isolate and determine by serological methods the extent of exposure in cattle. A preliminary study to determine the ability of parvovirus to cause abortion in cattle will also be undertaken.

Comparison Of The Direct Agglutination And Indirect Hemagglutination Tests In The Determination Of Blood Serum Titers To Escherichia Coli Organisms, I. A. Schipper, Clayton L. Kelling, H. Ebeltoft, D. Graves

Nebraska Center for Virology: Faculty Publications

A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies to Escherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming than for the direct agglutination test. Crude extract and purified polysaccharides were comparable as red blood cell sensitizing agents.

Application Of 125i To The Detection And Assay Of Viruses And Viral Antibody Adsorbed To Latex Particles, Charles Steven Duvall

Electronic Theses and Dissertations

This study, then, was undertaken to develop a universal, in vitro, extracellular method utilizing fluorescence and radioactive ¹²⁵I to rapidly detect, identify, and quantitate pathogenic viruses and viral antibodies. Focus of the study is the CLAM model, which consists of inert latex particles, plus four radioactively labeled components: antibody, virus, enzyme (RNase) and fluorochrome (FITC).

New Laboratory For Virus Disease Studies, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Exotic diseases continually pose a threat to Australia's animal industries and the consequences of major outbreak could be disastrous.

If an outbreak should occur, it is essential that clinical diagnosis is confirmed without delay for control measures to be effective.

The completion of the new Virology Laboratory provides a building in which most exotic viral diseases could be handled with safety for diagnostic purposes in the event of an outbreak.

Properties Of Salmonella Phage P3 After Subjection To Host-Controlled Modification By Passage Through E. Coli K-12, Benjamin J. Siapco

Loma Linda University Electronic Theses, Dissertations & Projects

Five new bacteriophages were isolated from lysogenic S. potsdam in this laboratory. Group B phages (P3 and P9a) were found to have unique properties, including their capability to infect strains of E. coli K-12 and E. coli C⁺/L. Passage of the phages through these hosts modified them (host-controlled modification) so that their ability to infect their original host, S. potsdam, was reduced and only 1-4 per million were able to form plaques.

To help elucidate the biochemical basis of host-controlled modification in the Salmonella potsdam group of bacteriophages, the clear plaque mutant of one of them, P3-c …

Barley Yellow Dwarf Virus In Western Australia, W A. Shipton

Journal of the Department of Agriculture, Western Australia, Series 4

BARLEY yellow dwarf virus disease was first positively identified in Western Australia in 1961.

Records indicate that symptoms approximating those later found to be caused by this disease were reported as early as 1936.

Swine Fever And African Swine Fever, V W. Smith

Journal of the Department of Agriculture, Western Australia, Series 4

PIGS may become infected by viruses which also cause disease in other animals (for instance foot-and-mouth disease) but there are two virus diseases worthy of special mention because they cause clinical disease in pigs only.

A Comparative Study Of The Growth Of Polyoma Virus In Two Secondary Cell Lines: Mouse Lymphoma And Normal Mouse Fibroblasts, Rheeta M. Stecker

Loma Linda University Electronic Theses, Dissertations & Projects

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D₁, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released …

Common Bean Mosaic, W P. Cass Smith

Journal of the Department of Agriculture, Western Australia, Series 4

COMMON bean mosaic is a widespread disease which attacks both dwarf and pole bean varieties.

The disease is caused by an infective principle or virus which spreads throughout the sap of affected plants, and causes both delayed maturity and drastic reduction in yield.

Replicating Single-Cycle Adenovirus Vectors Generate Amplified Influenza Vaccine Responses, Catherine M. Crosby, William E. Matchett, Stephanie S. Anguiano-Zarate, Christopher A. Parks, Eric A. Weaver, Larry R. Pease, Richard J. Webby, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Head-to-head comparisons of conventional influenza vaccines with adenovirus (Ad) gene-based vaccines demonstrated that these viral vectors can mediate more potent protection against influenza virus infection in animal models. In most cases, Ad vaccines are engineered to be replication-defective (RD-Ad) vectors. In contrast, replication-competent Ad (RC-Ad) vaccines are markedly more potent but risk causing adenovirus diseases in vaccine recipients and health care workers. To harness antigen gene replication but avoid production of infectious virions, we developed “single-cycle” adenovirus (SC-Ad) vectors. Previous work demonstrated that SC-Ads amplify transgene expression 100-fold and produce markedly stronger and more persistent immune responses than RD-Ad vectors …