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Articles 1 - 11 of 11
Full-Text Articles in Virology
Processing And Localization Of Dengue Virus Type 2 Polyprotein Precursor Ns3-Ns4a-Ns4b-Ns5, Luwen Zhang, P. Maruthi Mohan, R. Padmanabhan
Processing And Localization Of Dengue Virus Type 2 Polyprotein Precursor Ns3-Ns4a-Ns4b-Ns5, Luwen Zhang, P. Maruthi Mohan, R. Padmanabhan
Nebraska Center for Virology: Faculty Publications
Processing of dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5 could be mediated by the catalytically active NS3 protease domain and NS2B in trans at the dibasic sites NS3-NS4A and NS4B-NS5. Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5.
Loss Of Infectivity By Progeny Virus From Alpha Interferon- Treated Human Immunodeficiency Virus Type 1-Infected T Cells Is Associated With Defective Assembly Of Envelope Gp120, Brian D. Hanson, Peter L. Nara, Radha K. Maheshwari, Girmel S. Sidhu, John G. Bernbaum, David Hoekzema, Monte S. Meltzer, Howard Gendelman
Loss Of Infectivity By Progeny Virus From Alpha Interferon- Treated Human Immunodeficiency Virus Type 1-Infected T Cells Is Associated With Defective Assembly Of Envelope Gp120, Brian D. Hanson, Peter L. Nara, Radha K. Maheshwari, Girmel S. Sidhu, John G. Bernbaum, David Hoekzema, Monte S. Meltzer, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HlV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN-treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of a120 in aberrant cell compartments might affect HlV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to …
Worldwide Prevalence Of Lentivirus Infection In Wild Feline Species: Epidemiologic And Phylogenetic Aspects, Robert A. Olmstead, Raymond Langley, Melody E. Roelke, Robert M. Goeken, Diane Adger-Johnson, Julie P. Goff, John P. Albert, Craig Packer, M. Karen Laurenson, Tim M. Caro, Lue Scheepers, David E. Wildt, Mitchell Bush, Janice S. Martenson, Stephen J. O'Brien
Worldwide Prevalence Of Lentivirus Infection In Wild Feline Species: Epidemiologic And Phylogenetic Aspects, Robert A. Olmstead, Raymond Langley, Melody E. Roelke, Robert M. Goeken, Diane Adger-Johnson, Julie P. Goff, John P. Albert, Craig Packer, M. Karen Laurenson, Tim M. Caro, Lue Scheepers, David E. Wildt, Mitchell Bush, Janice S. Martenson, Stephen J. O'Brien
Biology Faculty Articles
The natural occurrence of lentiviruses closely related to feline immunodeficiency virus (FIV) in nondomestic felid species is shown here to be worldwide. Cross-reactive antibodies to FIV were common in several free-ranging populations of large cats, including East African lions and cheetahs of the Serengeti ecosystem and in puma (also called cougar or mountain lion) populations throughout North America. Infectious puma lentivirus (PLV) was isolated from several Florida panthers, a severely endangered relict puma subspecies inhabiting the Big Cypress Swamp and Everglades ecosystems in southern Florida. Phylogenetic analysis of PLV genomic sequences from disparate geographic isolates revealed appreciable divergence from domestic …
Identification And Characterization Of The Bovine Immunodeficiency-Like Virus Tat Gene, Zhen-Qian Liu, Deborah Sheridan, Charles Wood
Identification And Characterization Of The Bovine Immunodeficiency-Like Virus Tat Gene, Zhen-Qian Liu, Deborah Sheridan, Charles Wood
Nebraska Center for Virology: Faculty Publications
A cDNA clone of the bovine immunodeficiency-like virus (BW) trans-activator gene (tat) was identified and characterized. The tat cDNA clone was generated by splicing, and on the basis of sequence analysis, the Tat protein was found to be encoded entirely by the first exon. It is 103 amino acids in size and shares sequence homology with the human immunodeficiency virus (HW) Tat. The BIV tat clone can trans activate the BIV promoter effectively, as measured by the expression of the bacterial chloramphenicol acetyltransferase gene, when transfected into bovine cells. Besides activating the BIV promoter, the BIV Tat can also trans …
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Chemistry & Biochemistry Theses & Dissertations
The human immunodeficiency virus (HIV) reverse transcriptase (RT) (EC 2.7.7.49) is the central replication enzyme for HIV. In general, the kinetic mechanism for this and all other polymerases involves the ordered binding of two substrates: a primer-template (PT) followed by a deoxyribonucleoside triphosphate (dNTP). Previous investigations prompted this research when it was discovered that the substrate dNTP, in absence of PT, could protect a recombinant c-terminal mutant HIV-1 RT from inhibition by pyridoxal-5'-monophosphate (PLP), an active-site dNTP inhibitor. In contrast, the non-mutant recombinant HIV-1 RT required both substrates for protection from PLP inhibition. This investigation sought to determine if this …
Dual Regulation Of Silent And Productive Infection In Monocytes By Distinct Human Immunodeficiency Virus Type 1 Determinants, Howard Gendelman, Peter Westervelt, Timothy Henkel, David B. Trowbridge, Jan Orenstein, John Heuser, Lee Ratner
Dual Regulation Of Silent And Productive Infection In Monocytes By Distinct Human Immunodeficiency Virus Type 1 Determinants, Howard Gendelman, Peter Westervelt, Timothy Henkel, David B. Trowbridge, Jan Orenstein, John Heuser, Lee Ratner
Nebraska Center for Virology: Faculty Publications
The regulation of human immunodeficiency virus type 1 infection and replication in primary monocytes was investigated by mutagenesis of recombinant proviral clones containing an env determinant required for the infectivity of monocytes. Virus replication was assayed by determination of reverse transcriptase activity in culture fluids and by recovery of virus from monocytes following cocultivation with uninfected peripheral blood mononuclear cells. Three virus replication phenotypes were observed in monocytes: productive infection, silent infection, and no infection. Incorporation of the monocytetropic env determinant in a full-length clone incapable of infection or replication in primary monocytes (no infection) conferred the capacity for highly …
Cytolytic T Lymphocytes Specific For Tumors And Infected Cells From Mice With A Retrovirus-Induced Immunodeficiency Syndrome., Jennifer G. Erbe, Kathy A. Green, Karen M. Crassi, Herbert C. Morse, W R. Green
Cytolytic T Lymphocytes Specific For Tumors And Infected Cells From Mice With A Retrovirus-Induced Immunodeficiency Syndrome., Jennifer G. Erbe, Kathy A. Green, Karen M. Crassi, Herbert C. Morse, W R. Green
Dartmouth Scholarship
LP-BM5 retrovirus complex-infected C57BL/6 mice develop immunodeficiency, somewhat analogous to AIDS, termed murine AIDS (MAIDS). After secondary stimulation with syngeneic B-cell lymphomas from LP-BM5-infected mice, C57BL/6 mice produced vigorous CD8+ cytotoxic T lymphocytes specific for MAIDS-associated tumors. An anti-LP-BM5 specificity was suggested because spleen and lymph node cells from LP-BM5-infected mice served as target cells in competition assays, and cells from LP-BM5, but not ecotropic, virus-infected mice functioned as secondary in vitro stimulators to generate cytotoxic T lymphocytes to MAIDS tumors.
Transformation Of A Continuous Rat Embryo Fibroblast Cell Line Requires Three Separate Domains Of Simian Virus 40 Large T Antigen., Jiyue Zhu, Philip W. Rice, Lisa Gorsch, Marina Abate, Charles N. Cole
Transformation Of A Continuous Rat Embryo Fibroblast Cell Line Requires Three Separate Domains Of Simian Virus 40 Large T Antigen., Jiyue Zhu, Philip W. Rice, Lisa Gorsch, Marina Abate, Charles N. Cole
Dartmouth Scholarship
Mouse C3H 10T1/2 cells and the established rat embryo fibroblast cell line REF-52 are two cell lines widely used in studies of viral transformation. Studies have shown that transformation of 10T1/2 cells requires only the amino-terminal 121 amino acids of simian virus 40 (SV40) large T antigen, while transformation of REF-52 cells requires considerably more of large T antigen, extending from near the N terminus to beyond residue 600. The ability of a large set of linker insertion, small deletion, and point mutants of SV40 T antigen to transform these two cell lines and to bind p105Rb was determined. Transformation …
Identification And Characterization Of A Human Herpesvirus 6 Gene Segment That Trans Activates The Human Immunodeficiency Virus Type 1 Promoter, Yunqi Geng, Bala Chandran, Steven Josephs, Charles Wood
Identification And Characterization Of A Human Herpesvirus 6 Gene Segment That Trans Activates The Human Immunodeficiency Virus Type 1 Promoter, Yunqi Geng, Bala Chandran, Steven Josephs, Charles Wood
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) is a lymphotropic herpesvirus, and in vitro, HHV-6 can productively infect many of the same cell types as can human immunodeficiency virus (HIV). Coinfection by both viruses in vitro can lead to both activation of the HIV promoter and acceleration of cytopathic effects. We have previously demonstrated that a large, 22.25-kb cloned HHV-6 fragment, pZVB70, can trans activate HIV promoter expression in vitro. In this study, we show that the pZVB70 fragment can trans activate the HIV promoter in human T-cell lines as well as in the monkey kidney cell line CV-1. The pZVB70 insert was …
The Inhibitory Effects Of The Extracts Of Zingiber Plants On The Adsorption, Growth, And Replication Of Phage Lpp-1 In Cyanobacterium, Ebby Paul Jido
The Inhibitory Effects Of The Extracts Of Zingiber Plants On The Adsorption, Growth, And Replication Of Phage Lpp-1 In Cyanobacterium, Ebby Paul Jido
Master's Theses
No abstract provided.
Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti
Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti
Nebraska Center for Virology: Faculty Publications
Replication of human adenovirus type 12 DNA is blocked in abortively infected baby hamster kidney cells. The activity and accumulation of adenovirus 12 DNA polymerase is equivalent in infected hamster and human cell extracts. However, the accumulation of adenovirus type 12 DNA-binding protein is approximately 120-fold lower in extracts from infected hamster cells when compared to infected permissive human cells. This difference in accumulation is not because of replication of viral DNA during productive infection, since this difference is observed in the presence of hydroxyurea. The DNA-binding protein from infected hamster cells retains the ability to bind denatured DNA-cellulose. An …