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Pathogenic Microbiology Commons

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Full-Text Articles in Pathogenic Microbiology

A Lipidomics Approach To The Viral-Host Dynamics Of The Unicellular, Eukaryotic Alga Chlorella Variabilis And Its Viral Pathogen, Pbcv-1, Suzanne Rose Dec 2015

A Lipidomics Approach To The Viral-Host Dynamics Of The Unicellular, Eukaryotic Alga Chlorella Variabilis And Its Viral Pathogen, Pbcv-1, Suzanne Rose

Dissertations and Theses in Biological Sciences

This thesis focuses on the sterol and sphingolipid composition in the unicellular, green alga Chlorella variabilis and the lipidomic changes that occur during viral infection. Using lipid analysis by mass spectrometry, we have identified the major sterol, ergosterol and sphingolipid, glucosyl inositol phosphoceramide (GIPC) as constituents of C. variabilis cell membranes. Sterols and sphingolipids have essential biological functions such as hormone-based signaling, plant defense, and apoptosis as well as critical roles in structural components of the cell and organelle membranes. In chapters two and three, we focus on the characterization of sterol composition among both freshwater and marine alga and ...


Rnasek Is A V-Atpase-Associated Factor Required For Endocytosis And The Replication Of Rhinovirus, Influenza A Virus, And Dengue Virus, Jill Perreira, Aaron Aker, George Savidis, Christopher R. Chin, William M. Mcdougall, Jocelyn M. Portmann, Paul Meraner, Miles Smith, Motiur Rahman, Richard E. Baker, Annick Gauthier, Michael Franti, Abraham L. Brass Aug 2015

Rnasek Is A V-Atpase-Associated Factor Required For Endocytosis And The Replication Of Rhinovirus, Influenza A Virus, And Dengue Virus, Jill Perreira, Aaron Aker, George Savidis, Christopher R. Chin, William M. Mcdougall, Jocelyn M. Portmann, Paul Meraner, Miles Smith, Motiur Rahman, Richard E. Baker, Annick Gauthier, Michael Franti, Abraham L. Brass

Open Access Articles

Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane protein, was needed for the replication of HRV, influenza A virus, and dengue virus. RNASEK localizes to the cell surface and endosomal pathway and closely associates with the vacuolar ATPase (V-ATPase) proton pump. RNASEK is required for endocytosis, and its depletion produces enlarged clathrin-coated pits (CCPs) at the cell surface. These enlarged CCPs contain endocytic cargo and are bound by the scissioning GTPase, DNM2. Loss of RNASEK alters the ...


Transduction As The Method Of Horizontal Gene Transfer Of The Staphylococcal Chromosomal Cassette Mec (Sccmec), Amber B. Sauder May 2015

Transduction As The Method Of Horizontal Gene Transfer Of The Staphylococcal Chromosomal Cassette Mec (Sccmec), Amber B. Sauder

Senior Honors Projects, 2010-current

Methicillin-resistant Staphylococcus aureus (MRSA) gains resistance to β-lactam antibiotics through a mutated penicillin binding protein (PBP2a) encoded on the SCCmec element. In combination with the recombinase encoded by ccr, these two genes are used as markers of the mobile genetic element (SCCmec). Due to recent increases in community acquired MRSA infections, the mechanisms of antibiotic resistance gene transfer have gained attention. Transduction, a method of horizontal gene transfer mediated by bacteriophage, is believed to be responsible for the movement of the SCCmec element. Recent studies have shown the transduction of the SCCmec element in clinical isolates; however, this study is ...


Characterization Of The Interactions Between Staphylococcal Phage 80 Alpha Scaffold And Capsid Proteins, Laura Klenow Jan 2015

Characterization Of The Interactions Between Staphylococcal Phage 80 Alpha Scaffold And Capsid Proteins, Laura Klenow

Theses and Dissertations

Staphylococcal phage 80α can serve as a helper bacteriophage for a family of mobile genetic elements called Staphylococcus aureus pathogenicity islands (SaPIs). The prototype island, SaPI1, is able to hijack the 80α capsid assembly process and redirect capsid formation to yield smaller, phage-like transducing particles carrying SaPI DNA. Capsid size redirection is accomplished through two SaPI1-encoded gene products, CpmA and an alternate scaffold protein, CpmB. The normal 80α scaffold and the SaPI1 CpmB scaffold share a small block of conserved residues at their C-termini, several of which had been shown to be essential for CpmB function. This led to the ...