Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- Analogs & derivatives (1)
- Bacterial (1)
- Biofilms (1)
- Biological Sciences (1)
- Biomedical Sciences (1)
-
- Biotransformation (1)
- COLSA (1)
- Confocal (1)
- Cyanobacteria (1)
- Cyclic GMP (1)
- Gene deletion (1)
- Gene library (1)
- Genetics (1)
- Growth & development (1)
- Interaction (1)
- Locomotion (1)
- Metabolic networks and pathways (1)
- Metabolism (1)
- Oyster (1)
- Pathogen (1)
- Physiology (1)
- Polysaccharides (1)
- Pseudomonas aeruginosa (1)
- Vibrio (1)
- Publication Type
Articles 1 - 2 of 2
Full-Text Articles in Pathogenic Microbiology
Deletion Mutant Library For Investigation Of Functional Outputs Of Cyclic Diguanylate Metabolism In Pseudomonas Aeruginosa Pa14, Dae-Gon Ha, Megan E. Richman, George A. O'Toole
Deletion Mutant Library For Investigation Of Functional Outputs Of Cyclic Diguanylate Metabolism In Pseudomonas Aeruginosa Pa14, Dae-Gon Ha, Megan E. Richman, George A. O'Toole
Dartmouth Scholarship
We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Investigating The Interactions Between Cyanobacteria And Vibrio Parahaemolyticus, Caroline E. Ward
Honors Theses and Capstones
One well-known pathogen that has been the topic of many recent studies is Vibrio parahaemolyticus, which causes thousands of foodborne illnesses a year, mostly from the ingestion of raw or undercooked oysters. It has been shown cyanobacteria can act as a long-term reservoir of Vibrio cholerae, another pathogenic Vibrio, by encasing the cells within mucilaginous sheaths during which Vibrios enter a viable but non-culturable state. In this study we investigated the interaction of V. parahaemolyticus with cyanobacteria to determine whether cyanobacteria aid in the longevity and survival of V. parahaemolyticus. We found that non-pathogenic V. parahaemolyticus …