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Pathogenic Microbiology Commons

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Full-Text Articles in Pathogenic Microbiology

Isolation And Characterization Of Protein Kinases From Chick Cells Infected With Avian Myeloblastosis Virus, Margaret Orth, Mark Stanchfield Apr 1982

Isolation And Characterization Of Protein Kinases From Chick Cells Infected With Avian Myeloblastosis Virus, Margaret Orth, Mark Stanchfield

Life and Environmental Sciences Undergraduate Theses

Protein kinases isolated from chick myeloblast cells infected with avian myeloblastosis virus were characterized and compared to protein kinases from the virus itself. A strong resemblance between the two kinase types is indicated, with respect to molecular weight and enzyme activity On the basis of these similarities, an implication may be made as to the origin of the viral kinases.


The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink, Beth Myhre Apr 1982

The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink, Beth Myhre

Life and Environmental Sciences Undergraduate Theses

Aleutian disease virus (ADV), a parvovirus, was treated with the enzymes trypsin, alpha-chymotrypsin (CT), pronase, deoxyribonuclease (DNase), and ribonuclease (RNase) by incubation at 37C for 60 min. Effects of enzyme treatment on the infectivity of ADV were determined by direct immunofluorescent assay, which showed the only significant drop in titer, about 2 logQ, from treatment with pronase, trypsin, and CT. A change of over 1 log-,g was found to be significant using this assay. A time course of digestion by trypsin and CT was followed through liquid scintillation counting (LSC) of S methionine-labeled Crandall feline kidney cells (CRFKs), both ...