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Full-Text Articles in Pathogenic Microbiology

Natural Vertical Transmission Of Wnv In Montana, Inderbir Bains Mar 2017

Natural Vertical Transmission Of Wnv In Montana, Inderbir Bains

Life and Environmental Sciences Undergraduate Theses

West Nile Virus (WNV) emerged and rapidly spread throughout the United States within several years. WNV is spread in Montana primarily by the mosquito vector Culex tarsalis. Horizontal transmission of WNV has been extensively studied, while little is known regarding vertical transmission. Previous research suggests vertical transmission is possible in controlled laboratory studies. This research attempts to document natural vertical transmission in Montana. Larvae were collected and analyzed for WNV via RTPCR. Results show no presence of natural vertical transmission. Culex larvae and adult male mosquito collection methods were insufficient, fluctuations in temperatures and variant rainfall were contributing factors. Future ...


Plasmid-Mediated Transference Of Multiple-Antibiotic Resistance Between Escherichia Coli Isolates In The Western Montana Region, Karyn Beiber Apr 2009

Plasmid-Mediated Transference Of Multiple-Antibiotic Resistance Between Escherichia Coli Isolates In The Western Montana Region, Karyn Beiber

Life and Environmental Sciences Undergraduate Theses

This thesis investigates the multiple drug resistant (MDR) characteristic within enteric Escherichia coli (E. coli) isolates and their ability to transfer this phenotype via resistant-plasmid (R-plasmid) conjugation. E. coli were isolated from bovine fecal samples in the western Montana region. These isolates were subjected to antibiotic resistance testing, then sorted and stored based on general resistance to two broad-spectrum antibiotics, ampicillin and chlorotetracycline. All isolates underwent molecular differentiation using RAPD PCR, then plasmid extraction using a Qiagen Maxi kit. These results were compared to determine if genetically similar isolates, as determined by RAPD PCR, were more likely to contain a ...


Prevalent Multiple Antibiotic Resistance Phenotypes In Commensal Escherichia Coli Isolated From Bovine Feces Of The Rocky Mountain Region Of Montana, Jacqueline Schmidt Apr 2009

Prevalent Multiple Antibiotic Resistance Phenotypes In Commensal Escherichia Coli Isolated From Bovine Feces Of The Rocky Mountain Region Of Montana, Jacqueline Schmidt

Life and Environmental Sciences Undergraduate Theses

The phenomenon of antibiotic resistance has lead to heightened discussion on how these adaptations may have arisen with such universality. Why antibiotic resistance occurs in an unpredictable manner within bacterial populations is poorly understood. It has been observed that resistance to many respective antibiotics is seen within the same organism as part of one phenotype. Each class of antibiotic has a distinct mode of action suggesting that adaptation to one antibiotic would not necessary illicit resistance to another. This study evaluates and outlines the environmental contributions to these multiple antibiotic resistance phenotypes present in bovine of the Rocky Mountain region ...


Influence Of Sex, Age, And Antibiotic Soap Use On The Number And Antibiotic Resistance Of Bacteria, Anna Bramucci Apr 2007

Influence Of Sex, Age, And Antibiotic Soap Use On The Number And Antibiotic Resistance Of Bacteria, Anna Bramucci

Life and Environmental Sciences Undergraduate Theses

This research dealt with factors that may impact the number of enteric and fecal coliform bacteria and the antibiotic resistance of the bacteria present on the hands of children ages one to 15. The targeted bacteria were the subset enteric bacteria, which cause potentially lethal cases of diarrhea in the United States and worldwide. Further exacerbating the mortality rate associated with enteric bacterial infections is the degree of antibiotic resistance acquired by the bacteria. The sterile bag method was used to acquire bacteria from the hands of children; the bacteria were counted and tested for degrees of antibiotic resistance using ...


Five Extracellular Proteins Of Group A Streptococcus: Antigenicity And Gene Expression, Elisha Stenseth Apr 2002

Five Extracellular Proteins Of Group A Streptococcus: Antigenicity And Gene Expression, Elisha Stenseth

Life and Environmental Sciences Undergraduate Theses

Five genes that encode novel extracellular proteins were previously identified through the genomic analysis of four Group A Streptococcus strains (serotypes Ml, M3, M5, and M18) (Reid et al., 2001). Four of the five proteins have an LPXTG amino acid motif at the carboxyterminus, a motif that covalently links extracellular proteins to the cell surface in many gram-positive pathogens. All of the genes encode proteins with a secretion signal sequence at the aminoterminus. In vivo expression was demonstrated with Western blots using sera obtained from 80 patients with invasive infections, non-invasive skin infections, pharyngitis, and acute rheumatic fever. Real-time reverse ...


Dna Hybridization Studies Of Borrelia Using Isolated Plasmid Dna To Probe Southern Blots Of Undigested Whole Dna And Pst 1 Digested Whole Dna, Jenifer Maloney Apr 1995

Dna Hybridization Studies Of Borrelia Using Isolated Plasmid Dna To Probe Southern Blots Of Undigested Whole Dna And Pst 1 Digested Whole Dna, Jenifer Maloney

Life and Environmental Sciences Undergraduate Theses

Borrelia spirochetes, the etiologic agents of Lyme disease, are unique among bacteria because they contain linear, rather than circular, molecules of DNA. In addition, a significant amount, sometimes up to 25%, of the bacteria’s genome, is in the form of plasmids, small pieces of DNA which can replicate autonomously and can contain functional genes. This study was undertaken to investigate the structure and origin of several unusually large plasmids in four isolates of Borrelia, HO14, BO23, VS116, and R100. Isolating the desired plasmids from the bacterial cells was accomplished through gel electrophoresis, making it possible to isolate the desired ...


Induced Aggregation Of Culture Cells By Measles Virus And Its Possible Link To Viral Dissemination, Walter Kemp Apr 1993

Induced Aggregation Of Culture Cells By Measles Virus And Its Possible Link To Viral Dissemination, Walter Kemp

Life and Environmental Sciences Undergraduate Theses

Measles Virus (MV) Enders and MV Edmonston-Zagreb (EZ) were grown in both U937 cells and PBLs to study their dissemination. The > presence of aggregation and formation of giant cells was observed in those cultures infected with MV EZ, while it was notably absent from cultures infected with the MV Enders. Believing this difference to be a possible link in the understanding of measles virus dissemination, further studies were conducted to test the idea that MV EZ may disseminate intracellularly, while MV Enders disseminates extracellularly. Gamma Gard was added to cultures to study its effects on the dissemination of the two ...


Characterization And Cloning Of Coxiella Burnetii, Janelle Mclean Apr 1992

Characterization And Cloning Of Coxiella Burnetii, Janelle Mclean

Life and Environmental Sciences Undergraduate Theses

Coxiella burnetii, an obligate intracellular parasite, is the etiological agent of the disease QFever, which can occur in acute and chronic forms. The acute form is rarely serious, but chronic Q-Fever may result in endocarditis, which is usually fatal. It is therefore of interest to determine which strains of Coxiella burnetii lead to the acute versus the endocarditis-associated sickness, and what the mechanisms involved in each are. This past summer at Rocky Mountain Laboratories in Hamilton, Montana, under the direction of Dr.Ted Hackstadt,I began to research the Coxiella burnetii 9-Mile strain, which is associated with the acute form ...


A Proposed Diagonostic Test For Lyme Disease, Steven Klepps Apr 1992

A Proposed Diagonostic Test For Lyme Disease, Steven Klepps

Life and Environmental Sciences Undergraduate Theses

Lyme disease is caused by the spirochete, Borrelia burgdorferi, which is most commonly transmitted to humans in the bite of a deer tick. Because it is a pathogenic bacterium, the correct choice of antibiotic as well as the timely application of this antibiotic kills the bacterium thus halting the effects of the disease. The main problem in treating patients is the lack of a consistent and accurate diagnostic test. Because of this problem, a diagnostic test for Lyme borreliosis has been developed by researchers at Rocky Mountain Laboratories in Hamilton, MT. To determine the accuracy of this immunoassay, various human ...


Development Of A Vaccinia Virus Expression System For Structural Proteins Of Aleutian Mink Disease Parvovirus, Bradley Berry Apr 1992

Development Of A Vaccinia Virus Expression System For Structural Proteins Of Aleutian Mink Disease Parvovirus, Bradley Berry

Life and Environmental Sciences Undergraduate Theses

Aleutian Mink Disease Parvovirus (ADV) belongs to the parvovirus family. Although ADV can be grown in tissue culture and has been cloned in procaryotic systems, a eucaryotic expression system was desired. This would allow the opportunity to examine viral synthesis in absence of ADV replication. I attempted to develop a eucaryotic cloning system using vaccinia virus to express the ADV virion protein genes. My goal was to clone a segment of a cDNA clone (pIB21), that coded for the open reading frame containing both virion protein-1 (VP1) and virion protein-2 (VP2) of ADV, into pSCll, a modified vaccinia virus co-insertion ...


A Preliminary Microbiological Survey Of Helena Area Water For The Presence Of Pseudomonas : Potentially Pathogenic Microorganisms, Patricia Palagi Apr 1991

A Preliminary Microbiological Survey Of Helena Area Water For The Presence Of Pseudomonas : Potentially Pathogenic Microorganisms, Patricia Palagi

Life and Environmental Sciences Undergraduate Theses

A preliminary survey of Helena water for the presence of Pseudomonas, a potentially pathogenic genus of bacteria, was undertaken from June through August 1990. Microbiological methods suited specifically for recognizing this genus were utilized. The details of those methods, the results obtained through their use, and various ways the survey could be expanded are presented here. A particular member of the genus, Pseudomonas aeruginosa, is also looked at in detail due to its growing importance as an opportunistic pathogen.


The Role Of Recombination In Gonococcal Pilin Gene Variation, Conley Lynch Apr 1991

The Role Of Recombination In Gonococcal Pilin Gene Variation, Conley Lynch

Life and Environmental Sciences Undergraduate Theses

Pili show remarkable variation in Neisserria gonorrhoeae. This variation is due to variable pilin, the primary protein component of pili. The variation in pilin is in turn due to events at the genetic level. Intragenic recombination (i.e., recombination from a homologous duplex located on the same chromosome) is one means by which variation could be brought about. The presence of the recBCD gene wpuld be consistent with such a mechanism since the presence of this gene is involved in recombinational events. This past summer at the Rocky Mountain Labs under the direction of Stuart Hill, I attempted to identify ...


Molecular Cloning And Characterization Of A 35 K Protein-Coding Gene That Is Coregulated With The Gene(S) For The Outer Membrane Protein P.Lla In Neisseria Gonorrhoeae, Kathryn Wells Apr 1989

Molecular Cloning And Characterization Of A 35 K Protein-Coding Gene That Is Coregulated With The Gene(S) For The Outer Membrane Protein P.Lla In Neisseria Gonorrhoeae, Kathryn Wells

Life and Environmental Sciences Undergraduate Theses

A gene which coregulates the gene(s) that codes for the outer membrane protein P.ll in the bacterium Neisseria gonorrhoeae was cloned and characterized. As the cell expresses the P.ll protein, it no longer expresses the second protein; however, as the cell turns off the expression of P.ll, it then makes the second protein. A library of strain MS11 of the bacterium was constructed using the Lambda ZAP bacteriophage vector. This library was screened using a monospecific antiserum for the second protein. Molecular characterization of the positive clones followed.


Susceptibility Of Bovine Cell Cultures To The Bovine Viral Diarrhea Virus, Garret Mcfarland Apr 1989

Susceptibility Of Bovine Cell Cultures To The Bovine Viral Diarrhea Virus, Garret Mcfarland

Life and Environmental Sciences Undergraduate Theses

The bovine viral diarrhea (BVD) virus causes bovine viral diarrhea-mucosal disease (BVD-MD). Bovine fetal epithelial cell cultures of the lung tissue (EBL), kidney tissue (EBK), endothelium of the heart (EBEH), cornea tissue (EBC), turbinate cells (EBT), spleen tissue (EBSP), intestinal villi (EBI) and skin tissue (EBSK) were tested for their susceptibility to the BVD virus. The serial titers of the BVD virus used for the inoculation of the cells were from 10'® to 10"®. The degree of susceptibility was the greatest in the EBI and the EBEH at a degree of 5.14 for both. The EBL showed a degree ...


Increased Production Of Pertussigen S1 Subunit Via Phage Expression And Insertion Of A Transcription Terminator, Bradley Mott Apr 1989

Increased Production Of Pertussigen S1 Subunit Via Phage Expression And Insertion Of A Transcription Terminator, Bradley Mott

Life and Environmental Sciences Undergraduate Theses

Increased production of the S1 subunit of the toxin secreted by Bordetella pertussis, the causitive agent of whooping cough, was attempted by two methods. The first method was to use phage to insert the sequence for the S1 subunit into IL coli so that this bacterium would produce the subunit. This method was used to replace an inefficient heat transduction method formerly used. The second method attempted to increase the S1 subunit production by inserting a termination sequence after the S1 subunit sequence. It was hoped that reading of the sequence would stop after the S1 sequence, disengage and go ...


Pilus Antigenic Variation And Protein Ii Composition In The Outer Membrane Of Neisseria Gonorrhoeae, A Case History Of Patient 016, James Sola Apr 1988

Pilus Antigenic Variation And Protein Ii Composition In The Outer Membrane Of Neisseria Gonorrhoeae, A Case History Of Patient 016, James Sola

Life and Environmental Sciences Undergraduate Theses

In this study several male volunteers were infected with piliated gonococci expressing none of the pH proteins. Urine samples were collected from the patients twice daily until the patient developed clinical symptoms of gonorrhea. The samples from each volunteer were then analyzed in order to characterize how pII expression varied while being present in a human host. Pili were also examined for diferences in molecular weight. My role was to study the reisolated gonococci from patient 016. Pili were examined by epitope mapping, and the sequences of four pilin genes taken from the last urine sample were determined.


Screening For The Presence Of Escherichia Coli 0157:H7 In Montana, Richard Minudri Ii Apr 1988

Screening For The Presence Of Escherichia Coli 0157:H7 In Montana, Richard Minudri Ii

Life and Environmental Sciences Undergraduate Theses

The presence of Escherichia coli 0157:H7, a pathogenic strain of bacteria associated with hemorrhagic colitis and hemolytic uremic syndrome, was screened for in Montana from May through September 1987. Microbiological and biochemical methods of testing were employed in order to determine presumptive and confirmatory isolates of this organism. These tests resulted in 3 confirmed isolates of 124 tested stools providing the first detection of Escherichia coli 0157:H7 ever in the state of Montana. Applications and ramifications of the problem of diagnosis of this bacterial strain, seriousness of disease, and the importance of screening for it are discussed.


The Effects Of Attenuated Bovine Viral Diarrhea Virus On Cultured Bovine Fetal Cells, Warner Mcfarland Apr 1987

The Effects Of Attenuated Bovine Viral Diarrhea Virus On Cultured Bovine Fetal Cells, Warner Mcfarland

Life and Environmental Sciences Undergraduate Theses

Lung, kidney, intestine, aorta, turbinate epithelium, endothelium, cornea, and spleen were extracted from bovine fetuses. These cells were grown into monolayers and inoculated with serial dilutions of BVD virus. After a 96-hr period, the effects of BVD virus upon these cell cultures were studied. Our results demonstrated a difference in the susceptibility of these cell cultures towards BVD virus. The intestinal cells were the most susceptible to BVD viral infection and the least susceptible were cornea cells. There was a 4.5-fold difference in the amount of viral particles needed to cause infection.


A Study Of The Determinants On Gp70, A Viral Envelope Glycoprotein, To Which Neutralizing Monoclonal Antibodies Bind, Swend Holland Apr 1986

A Study Of The Determinants On Gp70, A Viral Envelope Glycoprotein, To Which Neutralizing Monoclonal Antibodies Bind, Swend Holland

Life and Environmental Sciences Undergraduate Theses

Lake Casitas Ecotropic virus causes a hind limb paralysis when injected into newborn mice. The long term goal is to select mutants of the virus using monoclonal antibodies and mutagens. Two of the monoclonal antibodies produced against this virus are neutralizing and also bind to the protein part of gp70, a viral envelope glycoprotein. Because of this, they can be used to select mutant viruses. There is no significant binding inhibition between monoclonal 704 and either of the two neutralizing monoclonals. Therefore, monoclonal 704 can be used in a focal-immuno-assay with the neutralizing monoclonals to locate mutant viruses.


Adp-Ribosylation Of Bsa By Pertussigen Follows The Same Mechanism As Adp-Ribosylation Of Transducine, John Cabrera Apr 1986

Adp-Ribosylation Of Bsa By Pertussigen Follows The Same Mechanism As Adp-Ribosylation Of Transducine, John Cabrera

Life and Environmental Sciences Undergraduate Theses

Pertussigen is a toxin responsible for the ADP-ribosylation of the Adenylate cyclase system inhibitory component. It has been verified through polyacrylamide gel electrophoresis that the ADP-ribosylation occurs only if the reaction mixture is supplemented with ATP, NAD and DTT. There is also a linear relationship between time and the amount of protein which is ADP ribosylated. Pertussigen can also ADP-ribosylize Bovine Serum Albumin (BSA) which offers researchers an inexpensive way to study the action of pertussigen.


Isolation & Partial Characterization Of The Capsule Produced By Neisseria Gonorrhoeae, Patricia Sullivan Apr 1986

Isolation & Partial Characterization Of The Capsule Produced By Neisseria Gonorrhoeae, Patricia Sullivan

Life and Environmental Sciences Undergraduate Theses

The capsule of Neisseria gonorrhoeae strains JS1 and JS3 was extracted according to the procedure of Wolfgang Casper (1937). The extracted products were biologically and biochemically characterized. The extracts were comprised primarily of carbohydrate and other constituents detected are ribonucleic acids, protein, 1ipopolysaccharide, phosphorus, and 2-keto-3-deoxyoctonate. The presence of these impurities has greatly interfered with the identification of the specific polysaccharide components. Examination of Neisseria gonorrhoeae by scanning electron microscopy indicated that extracellular material was stabilized by low temperatures. In order to maximize the amount of extracted polysaccha ride, the bacterial suspensions were refrigerated whenever possible during the purification procedure.


A Comparative Study On The Effects Of Various Protease Treatments Upon The Structure, Immunogenicity, And Infectivity Of The Elementary Bodies Of Chlamydia Trachomatis (Strains L2 And D) And Chlamydia Psittaci (Strain Mn), Timothy Mcinnis Apr 1985

A Comparative Study On The Effects Of Various Protease Treatments Upon The Structure, Immunogenicity, And Infectivity Of The Elementary Bodies Of Chlamydia Trachomatis (Strains L2 And D) And Chlamydia Psittaci (Strain Mn), Timothy Mcinnis

Life and Environmental Sciences Undergraduate Theses

The effects of several proteases upon the structure, immunogenicity, and infectivity of the elementary bodies (EBs) of Chlamydia trachomatis (strains L2 and D) and ot C. psittaci (strain MN) was studied. Four major proteases were studied and ot these, thermolysin and proteinase K were extremely etticient in cleaving the chlamydial major outer membrane protein (MOMP) while trypsin and chymotrypsin demonstrated cleavage abilities to a more limited extent in all strains. Proteinase K had the added ettect ot cleaving the surtace-exposed proteins on the chlamydial outer wall to the extent that monomeric MOMP was no longer detectable by immunoblot procedures with ...


The Use Of Enzyme-Linked Immunosorbent Assays To Detect Antibodies Of The Colorado Tick Fever Virus, Charleen Harmon Apr 1985

The Use Of Enzyme-Linked Immunosorbent Assays To Detect Antibodies Of The Colorado Tick Fever Virus, Charleen Harmon

Life and Environmental Sciences Undergraduate Theses

An enzyme-linked immunosorbent assay was attempted to determine antibody titers of serum from potential Colorado tick fever victims. An exemplary assay of influenza A monoclonal antibodies worked very well. After turning to the elucidation of optimal reagent purifications and titrations, a high degree of non-specificity was found. The numerous non-specific reactions which were observed necessitate the need for further development of the technique by the State of Montana Health and Environmental Sciences virology department.


A Serological Study Of The Humoral Antibody Response Of (B10 .Ax A. Wysn) F1 Mice With Friend Virus-Induced Erythroleukemia To Sheep Erythrocyte Antigens, Cyndra Coffing Apr 1985

A Serological Study Of The Humoral Antibody Response Of (B10 .Ax A. Wysn) F1 Mice With Friend Virus-Induced Erythroleukemia To Sheep Erythrocyte Antigens, Cyndra Coffing

Life and Environmental Sciences Undergraduate Theses

Friend virus (FV) is a murine leukemia virus. Previous studies have indicated that a profound humoral immunosuppression accompanies the erythroleukemia caused by FV. The presence of antibodies to Friend virus and sheep erythrocytes in the plasma of erythroleukemic mice which are capable of mounting an immune response to FV was tested using agglutination and cell binding assays in order to determine if humoral immunosuppression exists in the bloodstream. Friend virus-infected mice exhibited the same antibody response as normal mice, so no immunosuppression was discovered. The immunosuppressive effects of the disease are under genetic control. A better understanding of immunosuppression effects ...


A Study Of S-1 Subunit Specific Monoclonal Antibodies On Digested Whole Pertussigen: The Major Endotoxin Of Bordetella Pertussis, Mark Calderwood Apr 1985

A Study Of S-1 Subunit Specific Monoclonal Antibodies On Digested Whole Pertussigen: The Major Endotoxin Of Bordetella Pertussis, Mark Calderwood

Life and Environmental Sciences Undergraduate Theses

Common electophoretic and electroblot methods were used to differentiate between possibly different epitopes on digested whole pertussigen. In this study, S-l subunit specific monoclonal antibodies were used. The results of these tests indicate that these monoclonal antibodies react with the same antigenic region and do not distinguish between any other epitopes that may be present on the S-l subunit. Further development and testing of monoclonal antibodies against the various subunits of pertussigen will be required to elucidate the nature and presence of other epitopes of pertussigen.


Comparison Of Three Strains Of Contagious Equine Metritis Organism By Polyacrylamide Gel Electrophoresis And Description Of Three Colonial Opacity Variants, Albert Olszewski Apr 1984

Comparison Of Three Strains Of Contagious Equine Metritis Organism By Polyacrylamide Gel Electrophoresis And Description Of Three Colonial Opacity Variants, Albert Olszewski

Life and Environmental Sciences Undergraduate Theses

The colonial morphology and molecular composition of 26 phenotypes of Contagious Equine Metritis Organisms (CEMO) were studied by stereomicroscopic examination and polyacrylamide gel electrophoresis. Twenty-three phenotypes had colonies that were round and smooth. Three phenotypes had colonies that were round and smooth with occasional fimbria projecting from the edge of the colonies. The consistency of all the colonies varied from friable and butyrous to viscid. Based on external observation in reflected and transmitted light, the 26 phenotypes were separated into 3 different types of colonial variants based on opacity. The major stained bands in the page profiles of all 26 ...


Isolation And Characterization Of Protein Kinases From Chick Cells Infected With Avian Myeloblastosis Virus, Margaret Orth, Mark Stanchfield Apr 1982

Isolation And Characterization Of Protein Kinases From Chick Cells Infected With Avian Myeloblastosis Virus, Margaret Orth, Mark Stanchfield

Life and Environmental Sciences Undergraduate Theses

Protein kinases isolated from chick myeloblast cells infected with avian myeloblastosis virus were characterized and compared to protein kinases from the virus itself. A strong resemblance between the two kinase types is indicated, with respect to molecular weight and enzyme activity On the basis of these similarities, an implication may be made as to the origin of the viral kinases.


The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink, Beth Myhre Apr 1982

The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink, Beth Myhre

Life and Environmental Sciences Undergraduate Theses

Aleutian disease virus (ADV), a parvovirus, was treated with the enzymes trypsin, alpha-chymotrypsin (CT), pronase, deoxyribonuclease (DNase), and ribonuclease (RNase) by incubation at 37C for 60 min. Effects of enzyme treatment on the infectivity of ADV were determined by direct immunofluorescent assay, which showed the only significant drop in titer, about 2 logQ, from treatment with pronase, trypsin, and CT. A change of over 1 log-,g was found to be significant using this assay. A time course of digestion by trypsin and CT was followed through liquid scintillation counting (LSC) of S methionine-labeled Crandall feline kidney cells (CRFKs), both ...


Development And Evaluation Of A Simple Chemically Defined Minimal Growth Medium For The Detection And Identification Of Neisseria Gonorrhoeae, Anna Moudy Apr 1981

Development And Evaluation Of A Simple Chemically Defined Minimal Growth Medium For The Detection And Identification Of Neisseria Gonorrhoeae, Anna Moudy

Life and Environmental Sciences Undergraduate Theses

A simple chemically defined medium was developed and shown to he useful in the detection of nutritional mutants of Neisseria gonorrhoeae. These nutritional mutants are most often problematic organisms found in gonococcal infections. The procedure used in preparation of the medium will enable researchers to easily produce a specific medium in a relatively short time. Variations of the medium can be achieved through simple alterations in the composition of one of three stock solutions used.


Studies On The Isolation Of Campylobacter Fetus Subspecies Jejuni From Environmental Samples, Dale Tischmak Apr 1981

Studies On The Isolation Of Campylobacter Fetus Subspecies Jejuni From Environmental Samples, Dale Tischmak

Life and Environmental Sciences Undergraduate Theses

Various methods and samples were investigated for isolation of Campylobacter fetus subspecies jejuni. With the growing concern over intestinal infection due to Campylobacter fetus subspecies jejuni, discovery of its epidemiology has become increasingly important. The most likely source of infection is from ingested liquids, and results showed filtration followed by an inverted filter incubation to be the best method tested for recovery of the organisms.