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Pathogenic Microbiology Commons

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Full-Text Articles in Pathogenic Microbiology

The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin Nov 2016

The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin

Dissertations and Theses in Biological Sciences

Due to the increased number of immunocompromised patients, infections by Candida albicans have significantly increased in recent years. C. albicans transition from yeast to germ tubes is an essential factor for virulence. In this study we noted that Lee's medium, commonly used to induce filamentation, contained 500-fold more biotin than needed for growth. Thus, we investigated the effects of excess biotin on growth rate and filamentation by C. albicans in different media. At 37 °C, excess biotin (4 µM) enhanced germ tube formation (GTF) ca. 10-fold in both Lee's medium and a defined glucose proline medium, and ca ...


A Lipidomics Approach To The Viral-Host Dynamics Of The Unicellular, Eukaryotic Alga Chlorella Variabilis And Its Viral Pathogen, Pbcv-1, Suzanne Rose Dec 2015

A Lipidomics Approach To The Viral-Host Dynamics Of The Unicellular, Eukaryotic Alga Chlorella Variabilis And Its Viral Pathogen, Pbcv-1, Suzanne Rose

Dissertations and Theses in Biological Sciences

This thesis focuses on the sterol and sphingolipid composition in the unicellular, green alga Chlorella variabilis and the lipidomic changes that occur during viral infection. Using lipid analysis by mass spectrometry, we have identified the major sterol, ergosterol and sphingolipid, glucosyl inositol phosphoceramide (GIPC) as constituents of C. variabilis cell membranes. Sterols and sphingolipids have essential biological functions such as hormone-based signaling, plant defense, and apoptosis as well as critical roles in structural components of the cell and organelle membranes. In chapters two and three, we focus on the characterization of sterol composition among both freshwater and marine alga and ...


Chlorovirus Skp1 And Core Ankyrin-Repeat Protein Interplay And Mimicry Of Cellular Ubiquitin Ligase Machinery, Eric Andrew Noel Jun 2014

Chlorovirus Skp1 And Core Ankyrin-Repeat Protein Interplay And Mimicry Of Cellular Ubiquitin Ligase Machinery, Eric Andrew Noel

Dissertations and Theses in Biological Sciences

The ubiquitin-proteasome system is a common target of several unrelated viruses that have evolved convergent strategies to redirect host ubiquitin machinery to serve their own needs. Members of the genus Chlorovirus, a group of large dsDNA viruses that infect certain freshwater chlorella-like green algae, encode a conserved Skp1 homolog and ankyrin-repeat (ANK) proteins, some of which contain C-terminal domains characteristic of cellular F-boxes or related viral PRANC domains. These observations suggested that this unique combination of chlorovirus proteins either interact with or imitate the key components of the SCF (Skp1-Cul1-F-box) ubiquitin ligases. Using mass spectrometry, we identified two functional classes ...


Coccidia Of Gerbils From Mongolia, Ethan T. Jensen Aug 2013

Coccidia Of Gerbils From Mongolia, Ethan T. Jensen

Dissertations and Theses in Biological Sciences

In this study, gerbils collected in the Mongolia over the summers of 2009-2012 were examined for coccidia. In total, 171 gerbils of three species from 22 localities were examined for coccidia. Coccidian oocysts were identified from 21 gerbils, but those found in 1 of those gerbils were probably pseudoparasites of the host from which they were recovered. From the remaining 20 gerbils, 7 morphotypes of Eimeria and 1 morphotype of Isospora were identified. Four of the 7 morphotypes of Eimeria were attributed to new species which were described in this study. In addition, 10 previously described species of Eimeria were ...


The Pseudomonas Syringae Type Iii Secretion System: The Translocator Proteins, Their Secretion, And The Restriction Of Translocation By The Plant Immune System, Emerson Crabill Jul 2012

The Pseudomonas Syringae Type Iii Secretion System: The Translocator Proteins, Their Secretion, And The Restriction Of Translocation By The Plant Immune System, Emerson Crabill

Dissertations and Theses in Biological Sciences

Pseudomonas syringae is a Gram-negative plant pathogen whose virulence is dependent upon its type III secretion system (T3SS), a nanosyringe that facilitates translocation, or injection, of type III effector (T3E) proteins into eukaryotic cells. The primary function of P. syringae T3E proteins is suppression of plant immunity. Bacterial proteins called translocators form a translocon that forms a pore in the host plasma membrane which is traversed by T3Es. HrpK1, a putative P. syringae translocator, is a type III-secreted protein important for virulence and T3E injection, but not secretion of T3Es. Harpins are a group of proteins specific to plant pathogens ...


Farnesol Signaling In Candida Albicans, Melanie L. Langford Apr 2010

Farnesol Signaling In Candida Albicans, Melanie L. Langford

Dissertations and Theses in Biological Sciences

Candida albicans is a polymorphic fungus that causes a range of disease in humans, from mucosal infections to systemic disease. Its ability to cause disease is linked to conversion between yeast and filamentous forms of growth, and the first quorum-sensing molecule discovered in an eukaryote, farnesol, blocks this transition. In C. albicans, farnesol also kills mating-competent opaque cells, inhibits biofilm formation, protects the cells from oxidative stress, and can be a virulence factor or protective agent in disseminated and mucosal mouse models of infection, respectively. While much emphasis has been placed on determining its effect on C. albicans morphology, the ...