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Pathogenic Microbiology Commons

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Iowa State University

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Articles 1 - 12 of 12

Full-Text Articles in Pathogenic Microbiology

Lack Of Evidence For Erm(B) Infiltration Into Erythromycin-Resistant Campylobacter Coli And Campylobacter Jejuni From Commercial Turkey Production In Eastern North Carolina: A Major Turkey-Growing Region In The United States, Hannah K. Bolinger, Qijing Zhang, William G. Miller, Sophia Kathariou Aug 2018

Lack Of Evidence For Erm(B) Infiltration Into Erythromycin-Resistant Campylobacter Coli And Campylobacter Jejuni From Commercial Turkey Production In Eastern North Carolina: A Major Turkey-Growing Region In The United States, Hannah K. Bolinger, Qijing Zhang, William G. Miller, Sophia Kathariou

Veterinary Microbiology and Preventive Medicine Publications

In Campylobacter spp., resistance to erythromycin and other macrolides has typically implicated ribosomal mutations, especially substitutions in the 23S rRNA genes. However, in 2014, the macrolide resistance gene erm(B) was reported for the first time in Campylobacter and shown to be harbored by a multidrug resistance island in the chromosome of the swine-derived strain Campylobacter coli ZC113. erm(B)-positive C. coli and Campylobacter jejuni strains from the food supply have been mostly reported from China. However, erm(B)-positive C. coli isolates were also detected recently in fecal samples from turkeys in Spain. To determine whether erm(B ...


Changes In The Prevalence Of Salmonella Serovars Associated Swine Production And Correlations Of Avian, Bovine And Swine‐Associated Serovars With Human‐Associated Serovars In The United States (1997–2015), Chaohui Yuan, Adam C. Krull, Chong Wang, M. Erdman, P. J. Fedorka-Cray, C. M. Logue, Annette M. O'Connor Apr 2018

Changes In The Prevalence Of Salmonella Serovars Associated Swine Production And Correlations Of Avian, Bovine And Swine‐Associated Serovars With Human‐Associated Serovars In The United States (1997–2015), Chaohui Yuan, Adam C. Krull, Chong Wang, M. Erdman, P. J. Fedorka-Cray, C. M. Logue, Annette M. O'Connor

Veterinary Diagnostic and Production Animal Medicine Publications

As Salmonella enterica is an important pathogen of food animals, surveillance programmes for S. enterica serovars have existed for many years in the United States. Surveillance programmes serve many purposes, one of which is to evaluate alterations in the prevalence of serovars that may signal changes in the ecology of the target organism. The primary aim of this study was to evaluate changes in the proportion of S. enterica serovars isolated from swine over a near 20‐year observation period (1997–2015) using four longitudinal data sets from different food animal species. The secondary aim was to evaluate correlations between ...


Evaluate Broad Protection Of The Escherichia Coli Outer Membrane Protein (Omp) A Against Avian Pathogenic E. Coli Infections In Chickens, Caroline Treadwell Apr 2017

Evaluate Broad Protection Of The Escherichia Coli Outer Membrane Protein (Omp) A Against Avian Pathogenic E. Coli Infections In Chickens, Caroline Treadwell

Symposium on Undergraduate Research and Creative Expression

Avian Pathogenic E. coli (APEC) significantly impacts the poultry industry by causing the disease colibacillosis in chickens. In this research study, a vaccine containing Outer membrane protein A (OmpA), prevalent among APEC, was evaluated for its broad potential protectivity against APEC. The objectives of our study are to 1) evaluate whether an OmpA vaccine would stimulate an antibody response in chickens and 2) show in vitro broad protection against bacteria. At 4 days-old, White Leghorn chickens (9/group) were vaccinated subcutaneously twice at two week-intervals with either PBS (control) or 50 μg OmpA in CpG adjuvant. On day 33 of ...


Evaluation Of Escherichia Coli Outer Membrane Protein Etsc As A Candidate Antigen For Vaccine Against Avian Pathogenic E. Coli Infections In Chickens, David Couri Apr 2017

Evaluation Of Escherichia Coli Outer Membrane Protein Etsc As A Candidate Antigen For Vaccine Against Avian Pathogenic E. Coli Infections In Chickens, David Couri

Symposium on Undergraduate Research and Creative Expression

With the average American consuming 26 chickens per year, poultry is in high demand. Avian related infectious disease is rising and bacteria pose serious concerns for the health of livestock and possess zoonotic potential. The goal of the study is to evaluate surface protein EtsC highly prevalent among Avian Pathogenic E. coli (APEC), as a vaccine antigen candidate against these pathogens in chickens. The objective of this study is to determine antibody response in chickens vaccinated with the EtsC recombinant antigen. Eighteen 4 day-old White Leghorns (N=9/group) were subcutaneously vaccinated twice at two-week interval with PBS (control) or ...


Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, Heidi A. Wright, Byron F. Brehm-Stecher Dec 2016

Sodium Polyphosphate And Polyethylenimine Enhance The Antimicrobial Activities Of Plant Essential Oils, Heidi A. Wright, Byron F. Brehm-Stecher

Food Science and Human Nutrition Publications

Plant extracts have been used for millennia for treatment of disease, with much recent interest focusing on the antimicrobial activities of plant essential oils (EOs). Although EOs are active against common microbial pathogens, their effective use as topical, environmental, or food antimicrobials will require EO-based formulations with enhanced antimicrobial activities. In this study, two polyionic compounds, sodium polyphosphate (polyP, a polyanion) and polyethylenimine (PEI, a polycation), were evaluated for their abilities to enhance the antimicrobial activities of six EOs against the human pathogens Escherichia coli O157:H7, Salmonella enterica subsp. enterica ser. Minnesota, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and ...


Density-Dependent Prophylaxis In Crowded Beet Webworm, Loxostege Sticticalis (Lepidoptera: Pyralidae) Larvae To A Parasitoid And A Fungal Pathogen, Hailong Kong, Yunxia Cheng, Lizhi Luo, Thomas W. Sappington, Xingfu Jiang, Lei Zhang Jun 2013

Density-Dependent Prophylaxis In Crowded Beet Webworm, Loxostege Sticticalis (Lepidoptera: Pyralidae) Larvae To A Parasitoid And A Fungal Pathogen, Hailong Kong, Yunxia Cheng, Lizhi Luo, Thomas W. Sappington, Xingfu Jiang, Lei Zhang

Entomology Publications

Transmission of parasites and pathogens is generally positively density-dependent: as an insect population's density increases, the risk of an individual becoming attacked or infected also increases. In some insect species, individuals experiencing crowded conditions are more resistant to natural enemies than those experiencing low density conditions, and they are predicted to divert resources to increase resistance. This phenomenon is called density-dependent prophylaxis. Here, possible expression of prophylaxis in fifth-instar larvae of Beet Webworm, Loxostege sticticalis, to biocontrol agents was investigated under rearing densities of 1, 10, and 30 larvae per jar (650 mL). Larvae reared at the moderate density ...


Midgut Barrier Imparts Selective Resistance To Filarial Worm Infection In Culex Pipiens Pipiens, Michelle L. Michalski, Sarah M. Erickson, Bruce M. Christensen, Lyric C. Bartholomay Nov 2010

Midgut Barrier Imparts Selective Resistance To Filarial Worm Infection In Culex Pipiens Pipiens, Michelle L. Michalski, Sarah M. Erickson, Bruce M. Christensen, Lyric C. Bartholomay

Entomology Publications

Mosquitoes in the Culex pipiens complex thrive in temperate and tropical regions worldwide, and serve as efficient vectors of Bancroftian lymphatic filariasis (LF) caused by Wuchereria bancrofti in Asia, Africa, the West Indies, South America, and Micronesia. However, members of this mosquito complex do not act as natural vectors for Brugian LF caused by Brugia malayi, or for the cat parasite B. pahangi, despite their presence in South Asia where these parasites are endemic. Previous work with the Iowa strain of Culex pipiens pipiens demonstrates that it is equally susceptible to W. bancrofti as is the natural Cx. p. pipiens ...


Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, Andrew W. Lenssen, R. T. Lartey, T. C. Caesar-Tonthat, J. Eckhoff, S. L. Hanson, R. G. Evans Jan 2010

Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, Andrew W. Lenssen, R. T. Lartey, T. C. Caesar-Tonthat, J. Eckhoff, S. L. Hanson, R. G. Evans

Andrew W. Lenssen

Cercospora beticola, the causal agent of Cercospora leaf spot of sugar beet, survives as pseudostromata in infected sugar beet residues in the soil. Under optimal conditions, overwintering propagules germinate and produce conidia that are dispersed as primary inoculum to initiate infection in sugar beet. We developed a polymerase chain reaction (PCR) technique for rapid detection of C. beticola in field soils. Total DNA was first isolated from soil amended with C. beticola culture using the PowerSoil DNA Kit. The purified DNA was subjected to PCR in Extract- N-Amp PCR mix with CBACTIN primers over 35 cycles. The amplified products were ...


Improving Food Safety In Meat And Poultry: Will New Regulations Benefit Consumers?, Laurian J. Unnevehr, Tanya Roberts, Helen H. Jensen Jan 1997

Improving Food Safety In Meat And Poultry: Will New Regulations Benefit Consumers?, Laurian J. Unnevehr, Tanya Roberts, Helen H. Jensen

Economics Publications

The Centers for Disease Control and Prevention (CDC) state that "food­ borne disease remams one of the most common and important cases of illness and deaths" (Harman, et al., 1991). According to researchers at CDC and the U.S. Food and Drug Administration (FDA), 6.5 million to 33 million illnesses and up to 9,000 deaths may occur each year from foodborne pathogens (namely, bactena, parasites, virus­ es, and fungi). For just the few foodborne bacterial and parasitic diseases for which there are cost estimates, medical costs, and lost productivity cost society $6.5 to $35 bil­ lion annually ...


Sensitivity And Survival Of Escherichia Coli O157:H7 Isolates After X-Ray And Electron Beam Irradiation In Raw Ground Meat, Adelle Denise Chan Jan 1997

Sensitivity And Survival Of Escherichia Coli O157:H7 Isolates After X-Ray And Electron Beam Irradiation In Raw Ground Meat, Adelle Denise Chan

Retrospective Theses and Dissertations

No abstract provided.


[Beta]-Hemolysin And The Pathogenesis Of Serpulina Hyodysenteriae Infection, David Leonard Hutto Jan 1997

[Beta]-Hemolysin And The Pathogenesis Of Serpulina Hyodysenteriae Infection, David Leonard Hutto

Retrospective Theses and Dissertations

A murine model of Serpulina hyodysenteriae infection was utilized to explore the pathogenesis of lesion formation induced by that organism. Detailed characterization of gross and microscopic lesions in that model demonstrated that mice fed Teklad diet 85420 experienced the onset of severe gross and histologic lesions much sooner (within 24 hours) following infection with S. hyodysenteriae than did mice fed conventional rodent chow. However, gross and microscopic features of the induced lesions were no different in mice fed either diet.;The model was then used to document the progression of morphologic changes that occur in the cecal mucosa of mice ...


Determination Of Effect Of Sodium Ion Pretreatment On Escherichia Coli O157:H7 After Selected Organic Acid Treatments, Peggy Jeanette Wixom Jan 1994

Determination Of Effect Of Sodium Ion Pretreatment On Escherichia Coli O157:H7 After Selected Organic Acid Treatments, Peggy Jeanette Wixom

Retrospective Theses and Dissertations

No abstract provided.