Entomology Commons^™

Taxonomic Revision Of Perdita Subgenus Heteroperdita Timberlake (Hymenoptera: Andrenidae), With Descriptions Of Two Ant-Like Males, Zachary M. Portman, John L. Neff, Terry L. Griswold

Zachary Portman

Permian Scorpions From The Petrified Forest Of Chemnitz, Germany, Jason A. Dunlop, David A. Legg, Paul L. Selden, Victor Fet, Joerg W. Schneider, Ronny Rößler

Victor Fet

Background: Paleozoic scorpions (Arachnida: Scorpiones) have been widely documented from the Carboniferous Period; which hosts a remarkable assemblage of more than sixty species including both putative stem- and crown-group fossils. By contrast the succeeding Permian Period is almost completely devoid of records, which are currently restricted to a trace fossil from the early Permian of New Mexico, USA and some limb fragments from the late Permian of the Vologda Region, Russia. Results: ?Opsieobuthus tungeri sp. nov. from the Petrified Forest of Chemnitz, Germany represents the first complete body fossils of scorpions from the Permian. Explosive volcanism preserved these remarkable specimens …

The Forgotten Cousin In Freshwater Community Ecology: Tidal Freshwater Wetlands, Jack R. Mclachlan

Electronic Theses and Dissertations

Tidal freshwaters are unique in their placement in the landscape, forming where freshwater riverine flows are sufficient to overwhelm the saline water of the incoming tide, but not strong enough to suppress tidal water-height fluctuations. Tidal freshwaters and their wetlands have been overlooked by freshwater and marine researchers alike; neither discipline considers tidal freshwaters to fall under their purview. Invertebrate communities in tidal freshwaters are thought to be species depauperate; the consensus is that they support fewer taxa than nearby non-tidal freshwaters, but little is known about how these communities are structured. This study investigated how tidal hydrology, an atypical …

Invasive Species Impacts On Infrastructure, Invasive Species Advisory Committee

National Invasive Species Council

Executive Summary

Invasive species represent one of the most significant threats to ecosystems, human and animal health, infrastructure, the economy, and cultural resources. Because potentially invasive, non-native species typically enter the United States through ports of entry in urban environments, some of the first observable impacts may be to infrastructure, yet little is known about the economic costs associated with these impacts to the “built” environment. In addition, federal agencies currently lack the authority necessary to effectively prevent, eradicate, and control invasive species that impact the human-built environment. This lack of authority prevents rapid response to some of the most …

Insecticide Sensitivity Of Native Chloride And Sodium Channels In A Mosquito Cell Line, Lacey J. Jenson, Troy D. Anderson, Jeffrey R. Bloomquist

Department of Entomology: Faculty Publications

The aim of this study was to investigate the utility of cultured Anopheles gambiae Sua1B cells for insecticide screening applications without genetic engineering or other treatments. Sua1B cells were exposed to the known insecticidal compounds lindane and DIDS, which inhibited cell growth at micromolar concentrations. In patch clamp studies, DIDS produced partial inhibition (69%) of chloride current amplitudes, and an IC50 of 5.1 μM was determined for Sua1B cells. A sub-set of chloride currents showed no response to DIDS; however, inhibition (64%) of these currents was achieved using a low chloride saline solution, confirming their identity as chloride channels. In …

Resistance Of Rice Varieties To Sitophilus Oryzae (Coleoptera: Curculionidae), Diana Cristina Da Silva Costa, André Cirilo De Sousa Almeida, Marcio Da Silva Araújo, Elvis Arden Heinrichs, Mabio Chrisley Lacerda, José Alexandre Freitas Barrigossi, Flávio Gonçalves De Jesus

Department of Entomology: Faculty Publications

Abstract

Rice, Oryza sativa L. (Poaceae), is one of the world’s most important food crops. Among the insects that damage rice grains, the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), is the most important species. The objective of this study was to identify rice varieties with resistance to S. oryzae as measured by grain feeding damage and S. oryzae reproduction. The rice varieties evaluated were obtained from the germplasm bank of the National Center of Research Rice and Beans, Embrapa, Brazil. The experimental design was completely randomized with 3 replications and 22 treatments (varieties). The varieties Bonança, Esmeralda, and Rio …

Freeze Tolerance Of Cyphoderris Monstrosa (Orthoptera: Prophalangopsidae), Jantina Toxopeus, Jacqueline Lebenzon, Alexander H. Mckinnon, Brent J. Sinclair

Biology Publications

The great grig, Cyphoderris monstrosa Uhler (Orthoptera: Prophalangopsidae), is a large (20-30 mm, >1 g), nocturnal ensiferan that in habits montane coniferous forests in northwestern North America. C. monstrosa overwinters as a late-instar nymph, but its cold tolerance strategy has not previously been reported. We collected nymphs from near Kamloops, British Columbia, in late spring to determine their cold tolerance strategy. C. monstrosa nymphs were active at low temperatures until they froze at -4.6 ± 0.3 °C. The nymphs survived internal ice formation (i.e. are freeze tolerant), had a lethal temperature between -9 and -12 °C, and could survive for …

Novel Aspects Of Drosophila Suzukii (Diptera: Drosophilidae) Biology And An Improved Method For Culturing This Invasive Species With A Modified D. Melanogaster Diet, Blair J. Sampson, Trevor Mallette, Karla M. Addesso, Oscar E. Liburd, Lindsy E. Iglesias, Stephen J. Stringer, Chris T. Werle, Donna A. Shaw, Drew Larsen, John J. Adamczyk

Agricultural and Environmental Sciences Faculty Research

Drosophila suzukii (Matsumara) (Diptera: Drosophilidae), the spotted wing drosophila, is a global pest of soft fruits now rearable on a standard D. melanogaster (Meigen) diet containing the fly's own natural food: soft-skinned berries. The techniques tested here can save 40% of cultures from microbial contamination that develops after combining artificial food sources (e.g., standard drosophila media) with unsterilized host plant material (berries). A suitable ratio for mixing dietary ingredients for a vial or test-tube rearing system includes, by weight, 1 part berry tissue for oviposition, 1.5 parts dry diet media for carbohydrate, 7 parts clean water for moisture, and ∼5 …

Investigating The Functional Role Of Tick Antioxidants In Hematophagy And Vector Competence, Deepak Kumar

Dissertations

Ticks are obligate hematophagous arthropods and harbor several pathogens which transmit various diseases to humans and their domesticated animals. Host blood- digestion in a tick midgut (MG) generates several reactive oxygen species (ROS), which are extremely toxic to essential macromolecules (e.g. DNA, proteins, and lipids) within the cell, resulting in high oxidative stress. Thus, this dissertation focuses on the questions of how tick homeostasis responds to high oxidative stress, and how ticks and their harbored pathogens survive the high surge of oxidative stress during blood digestion. We are specifically interested in the tick-pathogen, Rickettsia parkeri (R. parkeri, Rp), harbored by …

Functional Diversity Enhances Detection Of Ecosystem Stability And Resolution Of Predator-Prey Interactions Within A Multitrophic Community, Ashley Lorraine Kissick

Open Access Dissertations

Habitat fragmentation and loss are principal factors that contribute to the decline of biodiversity which in turn has a negative impact on ecosystem function. There has been growing interest in understanding diversity’s role in the mechanisms behind ecosystem resilience with much attention focusing on how functional diversity, or the range of species’ ecological roles in a community, impacts ecosystem function. Under the functional insurance hypothesis, stability in ecosystems is maintained by species that perform similar functions but have asynchronous responses to disturbance. There are three proposed stability mechanisms that operate through species’ asynchronous responses: cross-scale resilience, response diversity, and density …

Billbug (Sphenophorus Spp.) Chemical Ecology And Seasonal Biology In Indiana Turfgrass, Alexandra G. Duffy

Open Access Theses

Billbugs (Coleoptera: Curculionoidea: Sphenophorus) are serious pests of managed turfgrass across North America. Damage symptoms are most visible during stressful periods of the growing season and are commonly confused with disease, drought, or nutrient deficiency. Billbugs are frequently a perennial problem and when misdiagnosed, damage often results in seriously degraded stands of turfgrass that are easily encroached by weeds. Presently, management of billbugs relies heavily on chemical insecticides. Even then, the nationwide assemblage of multiple sympatric billbug species and the cryptic nature of the damaging larval stage makes management of these insects challenging. A better understanding of billbug biology …

Investigations On The Vampire Moth Genus Calyptra Ochsenheimer, Incorporating Taxonomy, Life History, And Bioinformatics (Lepidoptera: Erebidae: Calpinae), Julia L. Snyder

Open Access Theses

The seventeen species and two subspecies described in the genus Calyptra are known to be obligate fruit piercers, with some species being of economic importance. Males within the genus have not only been observed piercing their fruit hosts, but have also been documented to occasionally feed on mammalian blood. The genetic and ecological mechanisms contributing to host preference for either plant or vertebrate hosts in this lineage are unknown. Thus, the focus of this study was to investigate the chemosensory systems between and among Calyptra species exhibiting differential feeding strategies. Before investigating the chemosensory systems within Calyptra, the taxonomy …

Rapid Molecular Detection And Population Genetics Of Pityophthorus Juglandis, A Vector Of Thousand Cankers Disease In Juglans Spp., Emel Oren

Masters Theses

Thousand Cankers Disease (TCD) is a disease complex involving the fungal pathogen Geosmithia morbida, an insect vector Pityophthorus juglandis, and the hosts, Juglans spp. and Pterocarya spp. Signs and symptoms of TCD include crown thinning due to branch dieback, yellowing and wilting of the leaves, appearance of epicormic shoots, numerous entrance/exit holes, gallery formation by P. juglandis, and the development of small, dark brown cankers underneath the bark. TCD originally described from western U.S., has now expanded to eastern U.S. and northwestern Italy. The disease complex is often difficult to diagnose due to the absence of symptoms …

Expression Of Glycine-Rich Proteins Found In Salivary Glands Of The Lone Star Tick (Amblyomma Americanum) Using A Mammalian Cell Line, Annabelle Clark

Honors Theses

Ticks play an important ecological role as well as a growing role in human health and veterinary care. Ticks are hosts to a plethora of microbial pathogens that can be transferred during feeding to cause tick-borne diseases in humans and many animals. Ticks may in large part owe the success of the transfer of these pathogens between hosts to their complex saliva. The saliva secreted upon a tick’s attachment to a host serves the following, among other, functions: anti-hemostasis of the blood pool, preventing an inflammatory response at the bite site, and serving as a natural anti-microbial substance. An important …

Survey Of Nasal Mites (Acari: Rhinonyssidae, Ereynetidae, Turbinoptidae) In Birds From Three States In The United States, Alexis Danel Hilario Perez

Graduate Theses and Dissertations

Avian nasal mites are obligate endoparasites that spend their entire life in the respiratory system of birds. In North America, bird nasal mites are represented by different unrelated families in three different orders of mites: Rhinonyssidae (Mesostigmata), Ereynetidae (Prostigmata), and the Cytoditidae and Turbinoptidae (Astigmata). The most-diverse and most-abundant family of nasal mites is the Rhinonyssidae, in which mite species may have different levels of host specificity from host orders to families or even species level. Nasal mites in North America have been surveyed in different locations, such as studies ranging from the Gulf Coast of the US to Canada. …

Integration Of Plant Defense Traits With Biological Control Of Arthropod Pests: Challenges And Opportunities, Julie A. Peterson, Paul J. Ode, Camila Oliveira-Hofman, James D. Harwood

Department of Entomology: Faculty Publications

Crop plants exhibit a wide diversity of defensive traits and strategies to protect themselves from damage by herbivorous pests and disease. These defensive traits may be naturally occurring or artificially selected through crop breeding, including introduction via genetic engineering. While these traits can have obvious and direct impacts on herbivorous pests, many have profound effects on higher trophic levels, including the natural enemies of herbivores. Multi-trophic effects of host plant resistance have the potential to influence, both positively and negatively, biological control. Plant defense traits can influence both the numerical and functional responses of natural enemies; these interactions can be …

Integration Of Plant Defense Traits With Biological Control Of Arthropod Pests: Challenges And Opportunities, Julie A. Peterson, Paul J. Ode, Camila Oliveira-Hofman, James D. Harwood

Entomology Faculty Publications

Crop plants exhibit a wide diversity of defensive traits and strategies to protect themselves from damage by herbivorous pests and disease. These defensive traits may be naturally occurring or artificially selected through crop breeding, including introduction via genetic engineering. While these traits can have obvious and direct impacts on herbivorous pests, many have profound effects on higher trophic levels, including the natural enemies of herbivores. Multi-trophic effects of host plant resistance have the potential to influence, both positively and negatively, biological control. Plant defense traits can influence both the numerical and functional responses of natural enemies; these interactions can be …

Predicting Post-Fire Change In West Virginia, Usa From Remotely-Sensed Data, Michael Strager P. Strager, Melissa Thomas-Van Gundy, Aaron E. Maxwell

Journal of Geospatial Applications in Natural Resources

Prescribed burning is used in West Virginia, USA to return the important disturbance process of fire to oak and oak-pine forests. Species composition and structure are often the main goals for re-establishing fire with less emphasis on fuel reduction or reducing catastrophic wildfire. In planning prescribed fires land managers could benefit from the ability to predict mortality to overstory trees. In this study, wildfires and prescribed fires in West Virginia were examined to determine if specific landscape and terrain characteristics were associated with patches of high/moderate post-fire change. Using the ensemble machine learning approach of Random Forest, we determined that …

Water Loss Rates And Desiccation Tolerances For Spiders And Crickets, Matthew Zach, Kevin E. Mccluney

Honors Projects

Despite the importance of water to living organisms, access varies across the globe with high variability over space and time. Seventy-five percent of the world’s freshwater alone is ice while 99% of unfrozen freshwater is underground (Winter et al. 1998). Understanding how terrestrial species respond to water availability and compensate for water stress can provide insight to their behavior, ecology and physiology. The goal of this research was to examine the differences in the evaporative water loss rates and desiccation tolerances of Hogna carolinensis and Acheta domesticus. First, an experiment was conducted to quantify the rate of evaporative water …

Genome Of The Asian Longhorned Beetle (Anoplophora Glabripennis), A Globally Significant Invasive Species, Reveals Key Functional And Evolutionary Innovations At The Beetle-Plant Interface, Duane D. Mckenna, Erin D. Scully, Yannick Pauchet, Kelli Hoover, Roy Kirsch, Scott M. Geib, Robert F. Mitchell, Robert M. Waterhouse, Seung-Joon Ahn, Deanna Arsala, Joshua B. Benoit, Heath Blackmon, Tiffany Bledsoe, Julia H. Bowsher, André Busch, Bernarda Calla, Hsu Chao, Anna K. Childers, Christopher Childers, Dave J. Clarke, Lorna Cohen, Jeffery P. Demuth, Huyen Dinh, Harshavardhan Doddapaneni, Amanda Dolan, Jian J. Duan, Shannon Dugan, Markus Friedrich, Karl M. Glastad, Michael A. D. Goodisman, Stephanie Haddad, Yi Han, Daniel S. T. Hughes, Panagiotis Ioannidis, J. Spencer Johnston, Jeffery W. Jones, Leslie A. Kuhn, David R. Lance, Chien-Yueh Lee, Sandra L. Lee, Han Lin, Jeremy A. Lynch, Armin P. Moczek, Shwetha C. Murali, Donna M. Muzny, David R. Nelson, Subba R. Palli, Kristen A. Panfilio, Dan Pers, Monica F. Poelchau, Honghu Quan, Jiaxin Qu, Ann M. Ray, Joseph P. Rinehart, Hugh M. Robertson, Richard Roehrdanz, Andrew J. Rosendale, Seunggwan Shin, Christian Silva, Alex S. Torson, Iris M. Vargas Jentzsch, John H. Werren, Kim C. Worley, George Yocum, Evgeny M. Zdobnov, Richard A. Gibbs, Stephen Richards

Entomology Faculty Publications

Background: Relatively little is known about the genomic basis and evolution of wood-feeding in beetles. We undertook genome sequencing and annotation, gene expression assays, studies of plant cell wall degrading enzymes, and other functional and comparative studies of the Asian longhorned beetle, Anoplophora glabripennis, a globally significant invasive species capable of inflicting severe feeding damage on many important tree species. Complementary studies of genes encoding enzymes involved in digestion of woody plant tissues or detoxification of plant allelochemicals were undertaken with the genomes of 14 additional insects, including the newly sequenced emerald ash borer and bull-headed dung beetle.

Results: …

Selection Of Reference Genes For Rt-Qpcr Analysis In Coccinella Septempunctata To Assess Un-Intended Effects Of Rnai Transgenic Plants, Chunxiao Yang, Evan L. Preisser, Hongjun Zhang, Yong Liu, Liangying Dai, Huipeng Pan, Xuguo Zhou

Entomology Faculty Publications

The development of genetically engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control. While this biotechnology shows tremendous promise, the potential for both non-target and off-target impacts, which likely manifest via altered mRNA expression in the exposed organisms, remains a major concern. One powerful tool for the analysis of these un-intended effects is reverse transcriptase-quantitative polymerase chain reaction, a technique for quantifying gene expression using a suite of reference genes for normalization. The seven-spotted ladybeetle Coccinella septempunctata, a commonly used predator in both classical and augmentative biological controls, is a …

Swimming Mechanisms Of Temperate Forest Ants, Noah D. Gripshover, Evan M. Gora, Stephen P. Yanoviak

Posters-at-the-Capitol

Swimming Mechanisms of Temperate Forest Ants (Camponotus pennsylvanicus and Formica subsericea)

Noah D. Gripshover, Evan M. Gora, and Stephen P. Yanoviak

University of Louisville

Abstract

Environmental challenges shape the evolution of animal behavior and morphology. For wingless terrestrial invertebrates like ants, pools of water on the forest floor are particularly dangerous. Here we show that ants can overcome this obstacle using a modified gait to transverse the water surface. We compared the locomotor morphology and swimming performance of two arboreal ant species that are common in Kentucky (Camponotus pennsylvanicus and Formica subsericea). We defined performance as speed …

Assessing The Risk Of Resistance Evolution, Adult And Larval Susceptibility, And Sublethal Effects After Exposure Of Corn Rootworms To Vacuolar Atpase-A And Snf7 Dsrnas, Adriano Elias Pereira

Department of Entomology: Dissertations, Theses, and Student Research

Corn rootworms are Diabroticite beetles from the genus Diabrotica and include the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, and the northern corn rootworm, D. barberi Smith & Lawrence, as the most important pests in the U.S. Corn Belt. The southern corn rootworm (SCR), D. undecimpunctata howardi Barber, is considered a polyphagous plant pest and feeds in a wide variety of plants including peanuts, cucurbits, soybeans, and especially corn. Resistance to control methods including behavioral resistance to crop rotation and to corn hybrids expressing Bacillus thuringiensis Berliner toxins in WCR has frequently been reported. SCR has been considered a …

Citations Increase With Manuscript Length, Author Number, And References Cited In Ecology Journals, Charles W. Fox, C. E. Timothy Paine, Boris Sauterey

Entomology Faculty Publications

Most top impact factor ecology journals indicate a preference or requirement for short manuscripts; some state clearly defined word limits, whereas others indicate a preference for more concise papers. Yet evidence from a variety of academic fields indicates that within journals longer papers are both more positively reviewed by referees and more highly cited. We examine the relationship between citations received and manuscript length, number of authors, and number of references cited for papers published in 32 ecology journals between 2009 and 2012. We find that longer papers, those with more authors, and those that cite more references are cited …

A New Species Of Psalidognathus Gray, 1831 From Peru (Coleoptera: Cerambycidae: Prioninae), Taketsune Noguchi, Antonio Santos-Silva

Insecta Mundi

Psalidognathus antonkozlovi (Coleoptera: Cerambycidae: Prioninae), a new species from Peru, is described and illustrated. The new species is included in a previous key.

Currently, Psalidognathus Gray, 1831 encompasses 10 species distributed from Costa Rica to northern South America (Monné 2016). The species can be included in two groups: the “modestus” group, species without a spine at the apex of the basal antennomeres; and the “friendii” group, species with a spine at the apex of the basal antennomeres. Santos-Silva and Komiya (2012) published a provisional study of the species of the “modestus” group, considering seven species in it. The key to …

Parajulid Milliped Studies Xi: Initial Assessment Of The Tribe Gosiulini (Diplopoda: Julida), Rowland M. Shelley, Jamie M. Smith

Insecta Mundi

The parajulid milliped tribe Gosiulini (Diplopoda: Julida) comprises two genera – Gosiulus Chamberlin, with three projections on the posterior gonopod and two species in the southcentral/southwestern United States (US) [Arizona, Colorado, New Mexico, and Texas], and monotypic Minutissimiulus Shelley, n. gen., with two projections, in Nuevo León, Mexico. Gosiulus conformatus Chamberlin occupies the plains/fl atlands of Texas, while its congener inhabits high elevations to the west in all four US states. Both are anticipated in Mexico (Coahuila, Chihuahua, and Sonora), and G. conformatus is expected in southeastern Colorado, eastern New Mexico, and the Oklahoma panhandle. The eastern boundary …

Euotophorus Imeldae New Genus And New Species Of Mexican Aphodiini (Coleoptera: Scarabaeidae: Aphodiinae), Marco Dellacasa, Giovanni Dellacasa, Robert D. Gordon

Insecta Mundi

Euotophorus new genus and E. imeldae new species (Coleoptera: Scarabaeidae: Aphodiinae) from Mexico are described and figured.

Annotated Checklist And Biogeographic Composition Of The Lycaenidae (Lepidoptera) Of Trinidad, West Indies, Matthew Cock, Robert K. Robbins

Insecta Mundi

A revised annotated checklist for the butterfl y family Lycaenidae (Lepidoptera) of Trinidad is presented, updating nomenclature, and indicating synonyms from earlier lists and papers. The checklist includes 131 species of Lycaenidae, comprising 127 species of 49 genera of Eumaeini, Theclinae, and four species of three genera of Polyommatinae. There are more than 30 new island records. No lycaenid species is endemic to Trinidad, and the fauna consists primarily of widespread species (71%) that occur from Central America to the Amazon Basin. However, the primary biogeographic affi nity is the Amazon Region, where 94% of the Trinidad lycaenid fauna also …

Juvenile Hormone Regulation Of Female Reproduction In The Common Bed Bug, Cimex Lectularius, Hemant Gujar, Subba Reddy Palli

Entomology Faculty Publications

To begin studies on reproduction in common bed bug, Cimex lectularius, we identified three genes coding for vitellogenin (Vg, a protein required for the reproductive success of insects) and studied their hormonal regulation. RNA interference studied showed that expression of Vg3 gene in the adult females is a prerequisite for successful completion of embryogenesis in the eggs laid by them. Juvenile hormone (JH) receptor, Methoprene-tolerant (Met), steroid receptor coactivator (SRC) and GATAa but not ecdysone receptor (EcR) or its partner, ultraspiracle (USP) are required for expression of Vg genes. Feeding and mating working through Vg, Met, SRC, EcR, and …

Comparative Profiling Of Micrornas In The Winged And Wingless English Grain Aphid, Sitobion Avenae (F.) (Homoptera: Aphididae), Xiangrui Li, Fangmei Zhang, Brad Coates, Yunhui Zhang, Xuguo Zhou, Dengfa Cheng

Entomology Faculty Publications

MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate gene expression, particularly during development. In this study, 345 miRNAs were identified from the English green aphid, Sitobion avenae (F.), of which 168 were conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA expression levels indicated that 16 and 12 miRNAs were significantly up-regulated in winged and wingless S. avenae small RNA libraries, respectively. Differential expression of these miRNAs was confirmed by real-time quantitative RT-PCR validation. The putative transcript targets for these candidate miRNAs were predicted based on sequences from a model species Drosophila melanogaster and four aphid species …