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Articles 1 - 15 of 15
Full-Text Articles in Cell and Developmental Biology
Heterochronic Genes Control The Stage-Specific Initiation And Expression Of The Dauer Larva Developmental Program In Caenorhabditis Elegans, Zhongchi Liu, Victor R. Ambros
Heterochronic Genes Control The Stage-Specific Initiation And Expression Of The Dauer Larva Developmental Program In Caenorhabditis Elegans, Zhongchi Liu, Victor R. Ambros
Victor R. Ambros
We report that a stage-specific developmental program, dauer larva formation, is temporally regulated by four heterochronic genes, lin-4, lin-14, lin-28, and lin-29. The effects of mutations in these four genes on dauer larva formation have revealed that they regulate two different processes of dauer larva formation: (1) a decision specifying the larval stage at which dauer larva development initiates, and (2) the specialized differentiation of hypodermal cells during dauer larva morphogenesis. Epistasis analysis has suggested a model in which lin-4 negatively regulates lin-14, and the resulting temporal decrease in lin-14 activity specifies the stage of dauer larva initiation. Our results …
Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little
Embryonic Chicken Fibroblast Collagen Binding Proteins: Distribution, Role In Substratum Adhesion, And Relationship To Integrins, Roy C. Ogle, A. Jeannette Potts, Marchall Yacoe, Charles D. Little
Medical Diagnostics & Translational Sciences Faculty Publications
Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment of cells adherent to a planar substratum. This process of antibody-mediated substratum detachment resulted in a clustering of CBP and cell-associated extracellular matrix at the cell surface, and the rearrangement of filamentous actin. Other functional studies showed that cells grown within a three-dimensional gel of type I collagen cannot be immunostained at the cell surface with CBP …
A New Kind Of Informational Suppression In The Nematode Caenorhabditis Elegans, Jonathan Hodgekin, Andrew Papp, Rock Pulak, Victor Ambros, Philip Anderson
A New Kind Of Informational Suppression In The Nematode Caenorhabditis Elegans, Jonathan Hodgekin, Andrew Papp, Rock Pulak, Victor Ambros, Philip Anderson
Victor R. Ambros
Independent reversions of mutations affecting three different Caenorhabditis elegans genes have each yielded representatives of the same set of extragenic suppressors. Mutations at any one of six loci act as allele-specific recessive suppressors of certain allels of unc-54 (a myosin heavy chain gene), lin-29 (a heterochronic gene), and tra-2 (a sex determination gene). The same mutations also suppress certain alleles of another sex determination gene, tra-1, and of a morphogenetic gene, dpy-5. In addition to their suppression phenotype, the suppressor mutations cause abnormal morphogenesis of the male bursa and the hermaphrodite vulva. We name these genes smg-1 through smg-6 (suppressor …
Effect Of Photoperiod On Developmental Morphology And Enolase Isoenzyme Immunohistochemistry In Rat And Djungarian Hamster Superficial Pineal Glands, Chalmer D. Mcclure
Effect Of Photoperiod On Developmental Morphology And Enolase Isoenzyme Immunohistochemistry In Rat And Djungarian Hamster Superficial Pineal Glands, Chalmer D. Mcclure
Loma Linda University Electronic Theses, Dissertations & Projects
The best understood functional activity of the pineal gland is its diurnal production of melatonin in response to environmental lighting cues. Several enzymes of the melatonin pathway respond to daily photoperiod changes, for example hydroxyindole-O-methyltransferase (HIOMT) and serotonin N-acetyltransferase (SNAT). Increased levels of the glycolytic enzyme neuron-specific enolase (NSE) are thought to reflect increased physiological demands placed on neurons and neuroendocrine tissues. Homodimer non-neuronal enolase isoenzyme (NNE) is immunolocalized to cells, and the hybrid enolase (consisting of subunits from NSE and NNE) has been seen in cerebellar stellate and basket cells. Although not rate limiting, concentrations of both NSE and …
Immunosuppressive Activity Derived From The Uc729-6 Lymphoblastoid And M21-Hpb Melanoma Cell Lines, Charlene J. Repique
Immunosuppressive Activity Derived From The Uc729-6 Lymphoblastoid And M21-Hpb Melanoma Cell Lines, Charlene J. Repique
Loma Linda University Electronic Theses, Dissertations & Projects
Previous work conducted in our laboratories, using a murine model, suggested that soluble factors secreted by tumor cells suppress lymphocyte responses. To apply this premise to human tumors, we studied the effects of UC729-6 (lymphoblastoid B-cell) and M21-HPB (malignant melanoma) conditioned media (CM) on lymphocyte proliferation assays, as well as on the growth of other cells. The CM was collected at 2-5 day intervals from cultures of UC729-6 and M21- HPB cells in serum-free media. In assays using phytohemagglutinin (PHA) and concanavalin A (ConA), mononuclear peripheral blood cells from healthy human donors showed decreased [3H]-thymidine ([3H]-Tdr) …
Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little
Collagen Binding Proteins Derived From The Embryonic Fibroblast Cell Surface Recognize Arginine-Glycine-Aspartic Acid, Roy C. Ogle, Charles D. Little
Medical Diagnostics & Translational Sciences Faculty Publications
Several cell surface proteins (Mr = 120,000, 90,000, 63,000 and 47,000) apparently integral to embryonic fibroblast plasma membranes were extracted with detergent and isolated by collagen affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000, and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist on the embryonic fibroblast cell surface. Some of the proteins may be collagen receptors binding to RGD sequences in the collagen molecule while at least one of the proteins (Mr = 63,000) recognizes …
A Hierarchy Of Regulatory Genes Controls A Larva-To-Adult Developmental Switch In C. Elegans, Victor Ambros
A Hierarchy Of Regulatory Genes Controls A Larva-To-Adult Developmental Switch In C. Elegans, Victor Ambros
Victor R. Ambros
The heterochronic genes lin-4, lin-14, lin-28, and lin-29 control the timing of specific postembryonic developmental events in C. elegans. The experiments described here examine how these four genes interact to control a particular stage-specific event of the lateral hypodermal cell lineages. This event, termed the "larva-to-adult switch" (L/A switch), involves several coordinate changes in the behavior of hypodermal cells at the fourth molt: cessation of cell division, formation of adult (instead of larval) cuticle, cell fusion, and cessation of the molting cycle. The phenotypes of multiply mutant strains suggest a model wherein the L/A switch is controlled by the stage-specific …
Hypoxanthine-Induced Differentiation Of Cultured Human Leukemia Cells, Gayle Jennette Singleton
Hypoxanthine-Induced Differentiation Of Cultured Human Leukemia Cells, Gayle Jennette Singleton
Chemistry & Biochemistry Theses & Dissertations
Human cultured leukemia cells appear to have a decreased amount of inosine in their tRNA. When cells with inosine deficient tRNA are placed in a hypoxanthine fortified media, they incorporate hypoxanthine into their tRNA by the action of the enzyme tRNA-hypoxanthine ribosyl transferase. This generates the nucleoside inosine in the tRNA. The cultured human leukemia cell lines, CCRF-CEM, HL-60, and HGPRT(-) HL- 60, incorporate hypoxanthine into their tRNA, as determined by tRNA isolation, hydrolysis, and HPLC analysis. Hypoxanthine treatment dramatically inhibited cell growth in conjunction with partial induction of differentiation in the CCRF-CEM, HL-60, and HGPRT ( - ) HL-60 …
Molecular Genetics Of The Caenorhabditis Elegans Heterochronic Gene Lin-14, Gary Ruvkun, Victor Ambros, Alan Coulson, Robert Waterston, John Sulston, H. Horvitz
Molecular Genetics Of The Caenorhabditis Elegans Heterochronic Gene Lin-14, Gary Ruvkun, Victor Ambros, Alan Coulson, Robert Waterston, John Sulston, H. Horvitz
Victor R. Ambros
We describe a general strategy for the genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical genetic loci within about 100 kb of any gene in Caenorhabditis elegans. We have used this strategy of parallel RFLP mapping to clone the heterochronic gene lin-14, which controls the timing and sequence of many C. elegans postembryonic developmental events. We found that of about 400 polymorphic loci in the C. elegans genome associated with the Tc1 family of repetitive elements, six are within 0.3 map unit of lin-14. The three …
A Flow Cytometric Approach To Assessing The Environmental And Physiological Status Of Phytoplankton, Serge Demers, Kimberly Davis, Terry L. Cucci
A Flow Cytometric Approach To Assessing The Environmental And Physiological Status Of Phytoplankton, Serge Demers, Kimberly Davis, Terry L. Cucci
CCPO Publications
No abstract provided.
An Electrochemical Method Of Measuring The Oxidation Rate Of Ferrous To Ferric Iron With Oxygen In The Presence Of Thiobacillus Ferrooxidans, David J. Oliver, B. Pesic, P. Wichlacz
An Electrochemical Method Of Measuring The Oxidation Rate Of Ferrous To Ferric Iron With Oxygen In The Presence Of Thiobacillus Ferrooxidans, David J. Oliver, B. Pesic, P. Wichlacz
David J. Oliver
The oxidation of Fe2+ with oxygen in sulfate solutions was studied in the presence of T. ferrooxidans. To measure the chemical activity of bacteria, and the oxidation rate of iron, the redox potentials of solutions were continuously monitored during the experiments. The redox potentials were simultaneously monitored on the platinum and pyrite indicator electrodes. The redox potential versus time curves were further used to calculate the basic kinetic parameters, such as the reaction orders, the activation energy, and the frequency factor. It was found that under atmospheric conditions, and at Fe2+ < 0.001M, T < 25°C, and at pH above 2.2, the oxidation of iron is governed by the following rate expression: [equation image] Below pH = 2.2, the oxidation rate is independent of H+ Concentration.
Cell Surface Charge Characteristics And Their Relationship To Bacterial Attachment To Meat Surfaces, James S. Dickson, M. Koohmaraie
Cell Surface Charge Characteristics And Their Relationship To Bacterial Attachment To Meat Surfaces, James S. Dickson, M. Koohmaraie
James S. Dickson
Cell surface charge and hydrophobicity of Bacillus subtilis, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis were determined by hydrocarbon adherence, hydrophobic interaction, and electrostatic interaction chromatography. Surface charge and hydrophobicity were compared with the initial attachment values and rates of attachment of the bacteria to meat surfaces. There was a linear correlation between the relative negative charge on the bacterial cell surface and initial attachment to lean beef muscle (r2 = 0.885) and fat tissue (r2 = 0.777). Hydrophobicity correlated well with attachment to fat tissue only. The relative hydrophobicity of each bacterium …
Characterization Of Pulmonary Surfactant Apoproteins In The Diabetic Mouse, Kenneth Dean Mccarty
Characterization Of Pulmonary Surfactant Apoproteins In The Diabetic Mouse, Kenneth Dean Mccarty
Theses Digitization Project
No abstract provided.
Photoreactivation Of The Effect Of Uv Light On Gamma Ray Induced Chromosome Aberration Production In G1 Phase Xenophus Cells, Rebecca Rowe, H. Gaston Griggs
Photoreactivation Of The Effect Of Uv Light On Gamma Ray Induced Chromosome Aberration Production In G1 Phase Xenophus Cells, Rebecca Rowe, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.
Photoreactivation Of Chromatid Deletions Induced By Uv-Irradiation Of G1 Phase Hamster X Xenophus Hybrid Cells, Joel Staggers, H. Gaston Griggs
Photoreactivation Of Chromatid Deletions Induced By Uv-Irradiation Of G1 Phase Hamster X Xenophus Hybrid Cells, Joel Staggers, H. Gaston Griggs
Journal of the Arkansas Academy of Science
No abstract provided.