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Full-Text Articles in Biology

Dna Extraction And Microsatellite Amplification Of Daphnia Pulicaria Resting Eggs: Analysis Of Allele Frequencies Through Time, Anna Ries Jan 2018

Dna Extraction And Microsatellite Amplification Of Daphnia Pulicaria Resting Eggs: Analysis Of Allele Frequencies Through Time, Anna Ries

Departmental Honors Projects

A paleoecological approach allows for the study of genetic change in populations over longer periods of time than would be possible if one were sampling populations from year to year. Daphnia and other cladoceran zooplankton are amenable to this type of study because they produce diapausing embryos (ephippial eggs) when they sexually reproduce, and these resting eggs can remain viable for decades to centuries in lake sediments. This study uses paleoecological methods as well as a new methodology for ephippial DNA extraction and amplification to assay for genetic variation in ephippial eggs obtained from sediments of varying ages from Square …


Uncovering Candidate Cold Tolerance Genes In Maize (Zea Mays), Raeann N. Goering Jan 2017

Uncovering Candidate Cold Tolerance Genes In Maize (Zea Mays), Raeann N. Goering

Departmental Honors Projects

With booming populations soon to overwhelm the world's food production capabilities, studying what makes crop organisms, like maize, efficient is crucial to ensure that the demand for food is met. Planting earlier in the spring would lengthen the crop season and produce larger yields provided the crop is tolerant to early spring’s low temperatures. Plants can adjust to abiotic stresses through biochemical changes controlled by transcription of genes. Trained plants can be produced by pre-exposing them to a lesser stress, allowing them to recover, then exposing them to a greater, longer stress. It is hypothesized that trained plants will tolerate …


Assessing The Use Of Food Waste Biochar As A Biodynamic Plant Fertilizer, Rachel Mazac Jan 2016

Assessing The Use Of Food Waste Biochar As A Biodynamic Plant Fertilizer, Rachel Mazac

Departmental Honors Projects

Biochar is a charcoal-like substance produced from plant material such as food waste. Converting food waste into a useful product would mitigate environmental damage through reduced landfill inputs, reduced greenhouse gas production, and increased benefits to soils. I asked (1) if biochar improved plant growth and (2) if the effects of biochar varied among different samples of mixed food waste (batches) and between different biochar preparation times (treatments). Four independent batches of biochar were prepared with assorted, uncooked food waste collected from a university dining facility. Each batch was dried then placed in a covered ceramic pot at 260℃ for …


Characterization Of Cp43 And Pif1 Gwas Selected Candidate Genes In Medicago Truncatula And Their Contribution To Rhizobia Symbiosis And Phenotypes Of Interest Using An Hprna Based Rnai Platform, Nathaniel Henning Jan 2015

Characterization Of Cp43 And Pif1 Gwas Selected Candidate Genes In Medicago Truncatula And Their Contribution To Rhizobia Symbiosis And Phenotypes Of Interest Using An Hprna Based Rnai Platform, Nathaniel Henning

Departmental Honors Projects

Medicago truncatula is a model organism used to study the symbiotic relationship between plants and nitrogen fixing soil bacteria of the genus rhizobia. By studying the genes that are involved in this symbiosis we can accomplish the goal of transplanting the symbiotic relationship into agriculture staples (e.g. corn, wheat, etc.) that do not utilize similar symbiotic relationships with nitrogen fixers. This would help to reduce our reliance on nitrogen-based fertilizers. In order to identify plant genes that might be important for establishing and regulating optimal nitrogen-fixing symbioses, a large-scale Genome Wide Association Study (GWAS) was performed. This study identified …