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Articles 1 - 30 of 395

Full-Text Articles in Biology

Characterizing Predator-Prey Relationships Of Great Lakes Fishes Using Targeted Stomach Content Dna Analyses, Matthew Richard Charron Nov 2023

Characterizing Predator-Prey Relationships Of Great Lakes Fishes Using Targeted Stomach Content Dna Analyses, Matthew Richard Charron

Electronic Theses and Dissertations

Stomach content DNA (scDNA) analyses provide important insights into feeding ecology, trophic interactions, and diet composition of predator fishes. However, previous scDNA analyses have focused on investigating a small number of predator and prey fishes using binary presence/absence data. Here, I describe the development and validation of a panel of single-species quantitative real-time PCR (qRT-PCR) assays targeting the polymorphic mitochondrial cytochrome oxidase subunit 1 (CO1) region of 28 Great Lakes prey fishes. The assay panel will significantly increase the number of targeted prey species available for predator diet assessment, while at the same time improving detection sensitivity and through-put. I …

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Microcystin Aids In Cold Temperature Acclimation: Differences Between A Toxic Microcystis Wildtype And Non-Toxic Mutant, Gwendolyn F. Stark, Robbie M. Martin, Laura E. Smith, Bofan Wei, Ferdi L. Hellweger, George S. Bullerjahn, R. Michael L. Mckay, Gregory L. Boyer, Steven W. Wilhelm Nov 2023

Microcystin Aids In Cold Temperature Acclimation: Differences Between A Toxic Microcystis Wildtype And Non-Toxic Mutant, Gwendolyn F. Stark, Robbie M. Martin, Laura E. Smith, Bofan Wei, Ferdi L. Hellweger, George S. Bullerjahn, R. Michael L. Mckay, Gregory L. Boyer, Steven W. Wilhelm

Great Lakes Institute for Environmental Research Publications

For Microcystis aeruginosa PCC 7806, temperature decreases from 26 °C to 19 °C double the microcystin quota per cell during growth in continuous culture. Here we tested whether this increase in microcystin provided M. aeruginosa PCC 7806 with a fitness advantage during colder-temperature growth by comparing cell concentration, cellular physiology, reactive oxygen species damage, and the transcriptomics-inferred metabolism to a non-toxigenic mutant strain M. aeruginosa PCC 7806 ΔmcyB. Photo-physiological data combined with transcriptomic data revealed metabolic changes in the mutant strain during growth at 19 °C, which included increased electron sinks and non-photochemical quenching. Increased gene expression was observed for …

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A Colonial-Nesting Seabird Shows Limited Heart Rate Responses To Natural Variation In Threats Of Polar Bears, Erica A. Geldart, Oliver P. Love, Andrew F. Barnas, Christopher M. Harris, H. Grant Gilchrist, Christina A.D. Semeniuk Oct 2023

A Colonial-Nesting Seabird Shows Limited Heart Rate Responses To Natural Variation In Threats Of Polar Bears, Erica A. Geldart, Oliver P. Love, Andrew F. Barnas, Christopher M. Harris, H. Grant Gilchrist, Christina A.D. Semeniuk

Great Lakes Institute for Environmental Research Publications

Several predator-prey systems are in flux as an indirect result of climate change. In the Arctic, earlier sea-ice loss is driving polar bears (Ursus maritimus) onto land when many colonial nesting seabirds are breeding. The result is a higher threat of nest predation for birds with potential limited ability to respond. We quantified heart rate change in a large common eider (Somateria mollissima) breeding colony in the Canadian Arctic to explore their adaptive capacity to keep pace with the increasing risk of egg predation by polar bears. Eiders displayed on average higher heart rates from baseline when polar bears were …

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Physiological And Behavioural Responses Of Arctic-Breeding Shorebirds To Weather And Habitat Conditions, Sara Bellefontaine Oct 2023

Physiological And Behavioural Responses Of Arctic-Breeding Shorebirds To Weather And Habitat Conditions, Sara Bellefontaine

Electronic Theses and Dissertations

Arctic-breeding shorebirds balance proximate and ultimate trade-offs in an energetically demanding environment. While these species are selective of nest sites, previous research shows that the characteristics of preferred shorebird nests do not predict increased breeding success. Thus, I aimed to identify detectable mechanisms driving nest preferences in Arctic-breeding shorebirds. Using an archival dataset, I first examined long-term nest preferences in relation to wind conditions at the East Bay Migratory Bird Sanctuary, on Southampton Island, Nunavut, Canada. I then studied energetic responses of two shorebird species to weather conditions across various nest types in a field study using non-invasive heart rate …

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Regulation Of Natural Killer Cell Responses By Myeloid-Derived Suppressor Cells In Mouse Mammary Tumors, Mary Ibrahim Sep 2023

Regulation Of Natural Killer Cell Responses By Myeloid-Derived Suppressor Cells In Mouse Mammary Tumors, Mary Ibrahim

Electronic Theses and Dissertations

Natural killer (NK) cells of the innate immune system play important roles in anti-cancer immunity. NK cell functions are regulated by inhibitory and activating receptors, which recognize specific ligands on the target cells. One example is the inhibitory NKR-P1B receptor which recognizes the ligand C-type lectin-related protein b (Clr-b). Work in our lab has shown NKR-P1B:Clr-b interactions are involved in mammary tumor immunosurveillance by NK cells. However, the cellular interactions and factors involved in modulating NK cell functions via this receptor:ligand system in the tumor microenvironment (TME) are not fully understood. This project aims to understand the role of myeloid-derived …

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Rapidly Developed, Optimized, And Applied Wastewater Surveillance System For Real-Time Monitoring Of Low-Incidence, High-Impact Mpox Outbreak, Chandler H. Wong, Zhihao Zhang, Walaa Eid, Julio Plaza-Diaz, Pervez Kabir, Shen Wan, Jian Jun Jia, Elisabeth Mercier, Ocean Thakali, Lakshmi Pisharody, Nada Hegazy, Sean E. Stephenson, Wanting Fang, Tram B. Nguyen, Nathan T. Ramsay, R. Michael Mckay, Ryland Corchis-Scott, Alex E. Mackenzie Sep 2023

Rapidly Developed, Optimized, And Applied Wastewater Surveillance System For Real-Time Monitoring Of Low-Incidence, High-Impact Mpox Outbreak, Chandler H. Wong, Zhihao Zhang, Walaa Eid, Julio Plaza-Diaz, Pervez Kabir, Shen Wan, Jian Jun Jia, Elisabeth Mercier, Ocean Thakali, Lakshmi Pisharody, Nada Hegazy, Sean E. Stephenson, Wanting Fang, Tram B. Nguyen, Nathan T. Ramsay, R. Michael Mckay, Ryland Corchis-Scott, Alex E. Mackenzie

Great Lakes Institute for Environmental Research Publications

Recent MPOX viral resurgences have mobilized public health agencies around the world. Recognizing the significant risk of MPOX outbreaks, large-scale human testing, and immunization campaigns have been initiated by local, national, and global public health authorities. Recently, traditional clinical surveillance campaigns for MPOX have been complemented with wastewater surveillance (WWS), building on the effectiveness of existing wastewater programs that were built to monitor SARS-CoV-2 and recently expanded to include influenza and respiratory syncytial virus surveillance in wastewaters. In the present study, we demonstrate and further support the finding that MPOX viral fragments agglomerate in the wastewater solids fraction. Furthermore, this …

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A Combined Microscopy And Single-Cell Sequencing Approach Reveals The Ecology, Morphology, And Phylogeny Of Uncultured Lineages Of Zoosporic Fungi, Kensuke Seto, D. Rabern Simmons, C. Alisha Quandt, Thijs Frenken, Alden C. Dirks, Rebecca A. Clemons, Katelyn M. Mckindles, R. Michael L. Mckay, Timothy Y. James Aug 2023

A Combined Microscopy And Single-Cell Sequencing Approach Reveals The Ecology, Morphology, And Phylogeny Of Uncultured Lineages Of Zoosporic Fungi, Kensuke Seto, D. Rabern Simmons, C. Alisha Quandt, Thijs Frenken, Alden C. Dirks, Rebecca A. Clemons, Katelyn M. Mckindles, R. Michael L. Mckay, Timothy Y. James

Great Lakes Institute for Environmental Research Publications

Environmental DNA analyses of fungal communities typically reveal a much larger diversity than can be ascribed to known species. Much of this hidden diversity lies within undescribed fungal lineages, especially the early diverging fungi (EDF). Although these EDF often represent new lineages even at the phylum level, they have never been cultured, making their morphology and ecology uncertain. One of the methods to characterize these uncultured fungi is a single-cell DNA sequencing approach. In this study, we established a large data set of single-cell sequences of EDF by manually isolating and photographing parasitic fungi on various hosts such as algae, …

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Exploring The Relationships Between Environmentally Influenced Foraging Behaviour, Energetics, And Breeding Decisions In Arctic Breeding Common Eiders, Erika Nissen Jun 2023

Exploring The Relationships Between Environmentally Influenced Foraging Behaviour, Energetics, And Breeding Decisions In Arctic Breeding Common Eiders, Erika Nissen

Electronic Theses and Dissertations

Migratory species breeding in polar environments face highly seasonal conditions and significant temporal and spatial constraints which can limit their ability to reproduce successfully. Effective energetic management is especially important at high latitudes, where a shortened breeding season and variable spring climatic conditions can restrict seasonal food availability, impact ability to initiate reproduction and successfully raise offspring. We explored this paradigm in marine benthic foraging common eiders (Somateria mollissima), at a long-term studied colonial nesting site at East Bay (Mitivik) Island, Nunavut, Canada. Eiders rely on marine prey to gain the energy necessary to invest in reproduction when arriving at …

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Heightened Heart Rate But Similar Flight Responses To Evolved Versus Recent Predators In An Arctic Seabird, Erica A. Geldart, Oliver P. Love, H. Grant Gilchrist, Andrew F. Barnas, Christopher M. Harris, Christina A.D. Semeniuk Jun 2023

Heightened Heart Rate But Similar Flight Responses To Evolved Versus Recent Predators In An Arctic Seabird, Erica A. Geldart, Oliver P. Love, H. Grant Gilchrist, Andrew F. Barnas, Christopher M. Harris, Christina A.D. Semeniuk

Great Lakes Institute for Environmental Research Publications

Predator-prey dynamics in the Arctic are being altered with changing sea ice phenology. The increasing frequency of predation on colonial nesting seabirds and their eggs by the polar bear (Ursus maritimus) is a consequence of bears shifting to terrestrial food resources through a shortened seal-hunting season. We examined antipredator responses in a colony of nesting Common Eiders (Somateria mollissima) on East Bay Island, Nunavut, Canada, which is exposed to established nest predators, such as arctic fox (Vulpes lagopus), but also to recent increases in polar bear nest predation due to the bears’ lost on-ice hunting opportunities. Given eiders’ limited eco-evolutionary …

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Sulfolipid Substitution Ratios Of Microcystis Aeruginosa And Planktonic Communities As An Indicator Of Phosphorus Limitation In Lake Erie, Robbie M. Martin, Maddie K. Denney, Helena L. Pound, Justin D. Chaffin, George S. Bullerjahn, R. Michael L. Mckay, Arthur Zastepa, Katarina A. Jones, Hector F. Castro, Shawn R. Campagna, Steven W. Wilhelm May 2023

Sulfolipid Substitution Ratios Of Microcystis Aeruginosa And Planktonic Communities As An Indicator Of Phosphorus Limitation In Lake Erie, Robbie M. Martin, Maddie K. Denney, Helena L. Pound, Justin D. Chaffin, George S. Bullerjahn, R. Michael L. Mckay, Arthur Zastepa, Katarina A. Jones, Hector F. Castro, Shawn R. Campagna, Steven W. Wilhelm

Great Lakes Institute for Environmental Research Publications

Phosphorus (P) availability frequently limits primary production in lakes, influences the physiology of phytoplankton, shapes community structure, and can stimulate or constrain the formation of cyanobacterial blooms. Given the importance of P, numerous methods are available to assess P stress in phytoplankton communities. Marine phytoplankton are known to substitute sulfolipids for phospholipids in response to P limitation. We asked whether sulfolipid substitution might serve as an additional indicator of P stress in freshwater phytoplankton communities. The question was addressed using cultures of Microcystis aeruginosa, Lake Erie microcosms, and surveys of lipid profiles in Lake Erie during a Microcystis spp. bloom. …

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Spatio-Temporal Connectivity Of The Aquatic Microbiome Associated With Cyanobacterial Blooms Along A Great Lake Riverine-Lacustrine Continuum, Sophie Crevecoeur, Thomas A. Edge, Linet Cynthia Watson, Susan B. Watson, Charles W. Greer, Jan J.H. Ciborowski, Ngan Diep, Alice Dove, Kenneth G. Drouillard, Thijs Frenken, Robert Michael Mckay, Arthur Zastepa, Jérôme Comte Feb 2023

Spatio-Temporal Connectivity Of The Aquatic Microbiome Associated With Cyanobacterial Blooms Along A Great Lake Riverine-Lacustrine Continuum, Sophie Crevecoeur, Thomas A. Edge, Linet Cynthia Watson, Susan B. Watson, Charles W. Greer, Jan J.H. Ciborowski, Ngan Diep, Alice Dove, Kenneth G. Drouillard, Thijs Frenken, Robert Michael Mckay, Arthur Zastepa, Jérôme Comte

Great Lakes Institute for Environmental Research Publications

Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the conditions that lead to bloom formation, such as assessing the physico-chemical and biological factors that influence the lake microbial community, as well as identifying the linkages between Lake Erie and the surrounding watershed. Within the scope of the Government of Canada’s Genomics Research and Development Initiative (GRDI) Ecobiomics project, we used high-throughput sequencing of the …

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Interactions Between Chytrids Cause Variable Infection Strategies On Harmful Algal Bloom Forming Species, Katelyn M. Mckindles, R. Michael L. Mckay, George S. Bullerjahn, Thijs Frenken Feb 2023

Interactions Between Chytrids Cause Variable Infection Strategies On Harmful Algal Bloom Forming Species, Katelyn M. Mckindles, R. Michael L. Mckay, George S. Bullerjahn, Thijs Frenken

Great Lakes Institute for Environmental Research Publications

Cyanobacteria have a great diversity of natural enemies, such as herbivores and pathogens, including fungal pathogens within the Chytridiomycota (chytrids). While these pathogens have been previously described on a select number of cyanobacterial hosts and are suspected to play a significant ecological role, little is understood about species interactions and how competition between parasites can affect epidemic development and bloom formation. Here, three Planktothrix agardhii isolates from Sandusky Bay, Lake Erie (OH, USA) were challenged in monoculture and polyculture against infection by three isolates (C1, C2, C10) of their obligate chytrid fungal pathogen, Rhizophydiales sp. The chytrid isolates were inoculated …

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Comparative Metabolomic Analysis Of Exudates Of Microcystin-Producing And Microcystin-Free Microcystis Aeruginosa Strains, Yuan Zhou, Jun Xu, Hugh J. Macisaac, Robert Michael Mckay, Runbing Xu, Ying Pei, Yuanyan Zi, Jiaojiao Li, Yu Qian, Xuexiu Chang Jan 2023

Comparative Metabolomic Analysis Of Exudates Of Microcystin-Producing And Microcystin-Free Microcystis Aeruginosa Strains, Yuan Zhou, Jun Xu, Hugh J. Macisaac, Robert Michael Mckay, Runbing Xu, Ying Pei, Yuanyan Zi, Jiaojiao Li, Yu Qian, Xuexiu Chang

Great Lakes Institute for Environmental Research Publications

Cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis aeruginosa threaten the ecological integrity and beneficial uses of lakes globally. In addition to producing hepatotoxic microcystins (MC), M. aeruginosa exudates (MaE) contain various compounds with demonstrated toxicity to aquatic biota. Previously, we found that the ecotoxicity of MaE differed between MC-producing and MC-free strains at exponential (E-phase) and stationary (S-phase) growth phases. However, the components in these exudates and their specific harmful effects were unclear. In this study, we performed untargeted metabolomics based on liquid chromatography-mass spectrometry to reveal the constituents in MaE of a MC-producing and a MC-free strain at …

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Targeting A Free Viral Fraction Enhances The Early Alert Potential Of Wastewater Surveillance For Sars-Cov-2: A Methods Comparison Spanning The Transition Between Delta And Omicron Variants In A Large Urban Center, Liang Zhao, Qiudi Geng, Ryland Corchis-Scott, Robert Michael Mckay, John Norton, Irene Xagoraraki Jan 2023

Targeting A Free Viral Fraction Enhances The Early Alert Potential Of Wastewater Surveillance For Sars-Cov-2: A Methods Comparison Spanning The Transition Between Delta And Omicron Variants In A Large Urban Center, Liang Zhao, Qiudi Geng, Ryland Corchis-Scott, Robert Michael Mckay, John Norton, Irene Xagoraraki

Great Lakes Institute for Environmental Research Publications

Introduction: Wastewater surveillance has proven to be a valuable approach to monitoring the spread of SARS-CoV-2, the virus that causes Coronavirus disease 2019 (COVID-19). Recognizing the benefits of wastewater surveillance as a tool to support public health in tracking SARS-CoV-2 and other respiratory pathogens, numerous wastewater virus sampling and concentration methods have been tested for appropriate applications as well as their significance for actionability by public health practices. Methods: Here, we present a 34-week long wastewater surveillance study that covers nearly 4 million residents of the Detroit (MI, United States) metropolitan area. Three primary concentration methods were compared with respect …

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Multi-Year Molecular Quantification And ‘Omics Analysis Of Planktothrix-Specific Cyanophage Sequences From Sandusky Bay, Lake Erie, Katelyn M. Mckindles, Makayla Manes, Michelle Neudeck, Robert Michael Mckay, George S. Bullerjahn Jan 2023

Multi-Year Molecular Quantification And ‘Omics Analysis Of Planktothrix-Specific Cyanophage Sequences From Sandusky Bay, Lake Erie, Katelyn M. Mckindles, Makayla Manes, Michelle Neudeck, Robert Michael Mckay, George S. Bullerjahn

Great Lakes Institute for Environmental Research Publications

Introduction: Planktothrix agardhii is a microcystin-producing cyanobacterium found in Sandusky Bay, a shallow and turbid embayment of Lake Erie. Previous work in other systems has indicated that cyanophages are an important natural control factor of harmful algal blooms. Currently, there are few cyanophages that are known to infect P. agardhii, with the best-known being PaV-LD, a tail-less cyanophage isolated from Lake Donghu, China. Presented here is a molecular characterization of Planktothrix specific cyanophages in Sandusky Bay. Methods and Results: Putative Planktothrix-specific viral sequences from metagenomic data from the bay in 2013, 2018, and 2019 were identified by two approaches: homology …

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Actionable Wastewater Surveillance: Application To A University Residence Hall During The Transition Between Delta And Omicron Resurgences Of Covid-19, Ryland Corchis-Scott, Qiudi Geng, Abdul Monem Al Riahi, Amr Labak, Ana Podadera, Kenneth K.S. Ng, Lisa A. Porter, Yufeng Tong, Jess C. Dixon, Sherri Lynne Menard, Rajesh Seth, R. Michael Mckay Jan 2023

Actionable Wastewater Surveillance: Application To A University Residence Hall During The Transition Between Delta And Omicron Resurgences Of Covid-19, Ryland Corchis-Scott, Qiudi Geng, Abdul Monem Al Riahi, Amr Labak, Ana Podadera, Kenneth K.S. Ng, Lisa A. Porter, Yufeng Tong, Jess C. Dixon, Sherri Lynne Menard, Rajesh Seth, R. Michael Mckay

Great Lakes Institute for Environmental Research Publications

Wastewater surveillance has gained traction during the COVID-19 pandemic as an effective and non-biased means to track community infection. While most surveillance relies on samples collected at municipal wastewater treatment plants, surveillance is more actionable when samples are collected “upstream” where mitigation of transmission is tractable. This report describes the results of wastewater surveillance for SARS-CoV-2 at residence halls on a university campus aimed at preventing outbreak escalation by mitigating community spread. Another goal was to estimate fecal shedding rates of SARS-CoV-2 in a non-clinical setting. Passive sampling devices were deployed in sewer laterals originating from residence halls at a …

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The Influence Of Administrative Timing In Triple-Negative Breast Cancer Treatments, Emily Mailloux Jan 2023

The Influence Of Administrative Timing In Triple-Negative Breast Cancer Treatments, Emily Mailloux

Electronic Theses and Dissertations

Breast cancer accounts for 25% of all cancers in Canadian women, and 15-20% of these are triple-negative breast cancers (TNBC), which have a poorer prognosis than other breast cancer subtypes. TNBC lacks expression of the estrogen receptor, progesterone receptor, and the human epidermal growth factor receptor 2 (HER2), which are common therapeutic targets in breast cancer. Due to the lack of target therapy, generalized chemotherapy treatments are used instead. The standard of care for treatment of TNBC instead consists of doxorubicin (A), cyclophosphamide (C) paclitaxel (T), and carboplatin (Carbo), that target various aspects of the cell cycle to induce cell …

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Examining The Effectiveness Of Probiotic Therapy For Improving Growth Performance Of Triploid Chinook Salmon (Oncorhynchus Tshawytscha) In Aquaculture Using A Behavioural Genomics Approach, Chelsea Frank Jan 2023

Examining The Effectiveness Of Probiotic Therapy For Improving Growth Performance Of Triploid Chinook Salmon (Oncorhynchus Tshawytscha) In Aquaculture Using A Behavioural Genomics Approach, Chelsea Frank

Electronic Theses and Dissertations

With an increasing human population, there has been increased production of fish to meet nutritional needs. Commercial aquaculture accounts for a significant portion of seafood production with salmonids being the major farmed finfish in Canada. To obtain greater biomass from aquaculture with minimal drawbacks (e.g., compromised flesh quality), triploidization has been implemented, altering ploidy from 2N to 3N, to induce sterility and promote energy investment towards somatic growth. Triploid individuals experience transcriptional and behavioural changes resulting in disease, mortalities, and reduced growth. Probiotic therapies (live microorganisms) have been recommended to potentially overcome drawbacks of triploidy and improve mass due to …

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Anthropogenic Land Uses Influence Stream Dissolved Organic Matter Quality More Than Decomposition Rates And Macroinvertebrate Diversity, Shayenna Nolan, Alyssa Alves Frazao, J D. Hosen, Catherine Febria Jan 2023

Anthropogenic Land Uses Influence Stream Dissolved Organic Matter Quality More Than Decomposition Rates And Macroinvertebrate Diversity, Shayenna Nolan, Alyssa Alves Frazao, J D. Hosen, Catherine Febria

Integrative Biology Publications

In the era of the Anthropocene, streams and rivers are among the most heavily impacted ecosystems due to the influence of catchment land use on stream water quality and ecological condition. In practice, structural and functional indicators collected by biomonitoring programs are underused and thus limited in their ability to offer practical insight into functional-based restoration approaches. Here we applied a novel combination of indicators—cotton strip decomposition, benthic invertebrate sampling together with dissolved organic matter (DOM) composition — to determine if streams highly impacted by urbanized and agricultural land use across Windsor-Essex (southwestern Ontario, Canada) were consistent across season, anthropogenic …

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The Role Of Cort And Anaphase Promoting Complex/Cyclosome (Apc/C) In Drosophila Sex Determination And Meiosis, Abuzar Sikander Malik Jan 2023

The Role Of Cort And Anaphase Promoting Complex/Cyclosome (Apc/C) In Drosophila Sex Determination And Meiosis, Abuzar Sikander Malik

Electronic Theses and Dissertations

The E3 ubiquitin ligase, APC/C, is essential for the completion cell cycle; along with its co-activators it allows mitotic exit and maintenance of G1. APC/C marks various substrates with ubiquitin chains; marked substrates are subsequently destroyed via the 26S proteasome pathway. Cort is a Drosophila female meiosis specific activator of APC/C. Cort works within meiosis in conjunction with Fzy to mediate Securin and cyclin destruction. A C-terminal IR-tail motif and a N-terminal C-box support Cort-APC/C interaction, whereas short motifs like D-box and KEN-box on the target protein impart substrate recognition to Cort. Cort expression is tightly controlled in the female …

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The Black Soldier Fly, Hermetia Illucens Linnaeus (Diptera: Stratiomyidae): A Novel Approach To Combat Food Waste In Windsor-Essex., Patricia Obianuju Okpara Jan 2023

The Black Soldier Fly, Hermetia Illucens Linnaeus (Diptera: Stratiomyidae): A Novel Approach To Combat Food Waste In Windsor-Essex., Patricia Obianuju Okpara

Electronic Theses and Dissertations

Within Ontario alone, approximately 3.7 million tonnes of organic food waste is generated yearly. This waste includes food scraps, wasted food, and plant wastes such as leaves, plant stems, and fruit. Most of it is transported to landfills for composting each year, but the use of landfills as a method of waste management is not sustainable. It adds strain to the environment by releasing harmful greenhouse gases and by demanding landfill space. The current protocols set by the Ontario government, while promising, do not address methods that support the safe disposal of organic waste and conversion into valuable end products …

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A Colonial-Nesting Seabird Shows No Heart-Rate Response To Drone-Based Population Surveys, Erica A. Geldart, Andrew F. Barnas, Christina A.D. Semeniuk, H. Grant Gilchrist, Christopher M. Harris, Oliver P. Love Dec 2022

A Colonial-Nesting Seabird Shows No Heart-Rate Response To Drone-Based Population Surveys, Erica A. Geldart, Andrew F. Barnas, Christina A.D. Semeniuk, H. Grant Gilchrist, Christopher M. Harris, Oliver P. Love

Great Lakes Institute for Environmental Research Publications

Aerial drones are increasingly being used as tools for ecological research and wildlife monitoring in hard-to-access study systems, such as in studies of colonial-nesting birds. Despite their many advantages over traditional survey methods, there remains concerns about possible disturbance effects that standard drone survey protocols may have on bird colonies. There is a particular gap in the study of their influence on physiological measures of stress. We measured heart rates of incubating female common eider ducks (Somateria mollissima) to determine whether our drone-based population survey affected them. To do so, we used heart-rate recorders placed in nests to quantify their …

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Technoscience And The Modernization Of Freshwater Fisheries Assessment And Management, S. J. Cooke, M. F. Docker, N. E. Mandrak, N. Young, D. D. Heath, K. M. Jeffries, A. Howarth, J. W. Brownscombe, J. Livernois, C. A.D. Semeniuk, P. A. Venturelli, A. J. Danylchuk, R. J. Lennox, I. Jarić, A. T. Fisk, C. S. Vandergoot, J. R. Britton, A. M. Muir Nov 2022

Technoscience And The Modernization Of Freshwater Fisheries Assessment And Management, S. J. Cooke, M. F. Docker, N. E. Mandrak, N. Young, D. D. Heath, K. M. Jeffries, A. Howarth, J. W. Brownscombe, J. Livernois, C. A.D. Semeniuk, P. A. Venturelli, A. J. Danylchuk, R. J. Lennox, I. Jarić, A. T. Fisk, C. S. Vandergoot, J. R. Britton, A. M. Muir

Great Lakes Institute for Environmental Research Publications

Inland fisheries assessment and management are challenging given the inherent complexity of working in diverse habitats (e.g., rivers, lakes, wetlands) that are dynamic on organisms that are often cryptic and where fishers are often highly mobile. Yet, technoscience is offering new tools that have the potential to reimagine how inland fisheries are assessed and managed. So-called “technoscience” refers to instances in which science and technology unfurl together, offering novel ways of spurring and achieving meaningful change. This paper considers the role of technoscience and its potential for modernizing the assessment and management of inland fisheries. It first explores technoscience and …

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Innovating Transcriptomics For Practitioners In Freshwater Fish Management And Conservation: Best Practices Across Diverse Resource-Sector Users, C. A.D. Semeniuk, K. M. Jeffries, T. Li, C. M. Bettles, S. J. Cooke, B. A. Dufour, E. A. Halfyard, J. W. Heath, K. Keeshig, N. E. Mandrak, A. J. Muir, L. Postma, D. D. Heath Sep 2022

Innovating Transcriptomics For Practitioners In Freshwater Fish Management And Conservation: Best Practices Across Diverse Resource-Sector Users, C. A.D. Semeniuk, K. M. Jeffries, T. Li, C. M. Bettles, S. J. Cooke, B. A. Dufour, E. A. Halfyard, J. W. Heath, K. Keeshig, N. E. Mandrak, A. J. Muir, L. Postma, D. D. Heath

Great Lakes Institute for Environmental Research Publications

Thriving freshwater fish populations contribute to people’s economic prosperity and wellbeing. Yet, freshwater fish populations are in critical condition around the globe. Most stressors to freshwater fishes, fisheries, and culture stem from habitat impacts, water-quality issues, and aquatic invasive species. Logistical difficulties of monitoring fish health are compounded by the limitations of conventional (capture-based) sampling methods, which provide only a temporal “snapshot” and generate data lacking in sensitivity and prognostic ability. Here, we propose an innovative genomics approach to develop a health toolkit that will allow resource-sector users to determine the health status of freshwater fishes, including their coping capacity, …

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Metatranscriptomic Sequencing Of Winter And Spring Planktonic Communities From Lake Erie, A Laurentian Great Lake, Brittany N. Zepernick, Elizabeth R. Denison, Justin D. Chaffin, George S. Bullerjahn, Christa P. Pennacchio, Thijs Frenken, Daniel H. Peck, James T. Anderson, Derek Niles, Arthur Zastepa, R. L.Michael Mckay, Steven W. Wilhelm Jul 2022

Metatranscriptomic Sequencing Of Winter And Spring Planktonic Communities From Lake Erie, A Laurentian Great Lake, Brittany N. Zepernick, Elizabeth R. Denison, Justin D. Chaffin, George S. Bullerjahn, Christa P. Pennacchio, Thijs Frenken, Daniel H. Peck, James T. Anderson, Derek Niles, Arthur Zastepa, R. L.Michael Mckay, Steven W. Wilhelm

Great Lakes Institute for Environmental Research Publications

Previous reports suggest planktonic and under-ice winter microbial communities in Lake Erie are dominated by diatoms. Here, we report the assembled metatranscriptomes of 79 Lake Erie surface water microbial communities spanning both the winter (28 samples) and spring (51 samples) months over spatial, temporal, and climatic gradients in 2019 through 2020.

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Metagenomics Of Wastewater Influent From Wastewater Treatment Facilities Across Ontario In The Era Of Emerging Sars-Cov-2 Variants Of Concern, Opeyemi U. Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly A. Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Sean Ma, Hanlan Mcdougall, R. Michael Mckay, Aleksandra Mloszewska, Claire Oswald, Mark Servos, Megan Swinwood-Sky Jul 2022

Metagenomics Of Wastewater Influent From Wastewater Treatment Facilities Across Ontario In The Era Of Emerging Sars-Cov-2 Variants Of Concern, Opeyemi U. Lawal, Linkang Zhang, Valeria R. Parreira, R. Stephen Brown, Charles Chettleburgh, Nora Dannah, Robert Delatolla, Kimberly A. Gilbride, Tyson E. Graber, Golam Islam, James Knockleby, Sean Ma, Hanlan Mcdougall, R. Michael Mckay, Aleksandra Mloszewska, Claire Oswald, Mark Servos, Megan Swinwood-Sky

Great Lakes Institute for Environmental Research Publications

We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.

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Niches Of Marine Mammals In The European Arctic, K. M. Mackenzie, C. Lydersen, T. Haug, H. Routti, J. Aars, C. M. Andvik, K. Borgå, A. T. Fisk, S. Meier, M. Biuw, A. D. Lowther, U. Lindstrøm, K. M. Kovacs Mar 2022

Niches Of Marine Mammals In The European Arctic, K. M. Mackenzie, C. Lydersen, T. Haug, H. Routti, J. Aars, C. M. Andvik, K. Borgå, A. T. Fisk, S. Meier, M. Biuw, A. D. Lowther, U. Lindstrøm, K. M. Kovacs

Great Lakes Institute for Environmental Research Publications

The Arctic is warming rapidly, with concomitant sea ice losses and ecosystem changes. The animals most vulnerable to Arctic food web changes are long-lived and slow-growing such as marine mammals, which may not be able to adapt rapidly enough to respond to changes in their resource bases. To determine the current extent and sources of these resource bases, we examined isotopic and trophic niches for marine mammals in the European Arctic using skin carbon (δ13C) and nitrogen (δ 15N) stable isotope (SI) compositions from 10 species: blue, fin, humpback, minke, sperm and white whales, bearded and ringed seals, walruses and …

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Bear Presence Attracts Avian Predators But Does Not Impact Lesser Snow Goose Daily Nest Attendance, Andrew F. Barnas, Brian J. Darby, David T. Iles, David N. Koons, Robert F. Rockwell, Christina A.D. Semeniuk, Susan N. Ellis-Felege Mar 2022

Bear Presence Attracts Avian Predators But Does Not Impact Lesser Snow Goose Daily Nest Attendance, Andrew F. Barnas, Brian J. Darby, David T. Iles, David N. Koons, Robert F. Rockwell, Christina A.D. Semeniuk, Susan N. Ellis-Felege

Great Lakes Institute for Environmental Research Publications

The presence of foraging bears in Arctic breeding bird colonies has been increasingly reported in the literature, and these may constitute disturbance events which cause incubating birds to leave their nest. Avian predators may associate with bears during such events, likely to capitalize on unattended nests in the presence of bears. Here, we estimated changes in daily nest attendance of lesser snow geese Anser caerulescens caerulescens in the presence of foraging bears, and estimated the association between foraging bears and avian predators. We predicted decreased nest attendance by geese on days with bears, and close associations between avian predators and …

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Corrigendum To “Environmental And Life-History Factors Influence Inter-Colony Multidimensional Niche Metrics Of A Breeding Arctic Marine Bird” [Sci. Total Environ. 796 (2021) 148935] (Science Of The Total Environment (2021) 796, (S0048969721040079), (10.1016/J.Scitotenv.2021.148935)), Reyd A. Smith, David J. Yurkowski, Kyle J.L. Parkinson, Jérôme Fort, Holly L. Hennin, H. Grant Gilchrist, Keith A. Hobson, Mark L. Mallory, Paco Bustamante, Jóhannis Danielsen, Svend E. Garbus, Sveinn A. Hanssen, Jón Einar Jónsson, Christopher J. Latty, Ellen Magnúsdóttir, Børge Moe, Glen J. Parsons, Christian Sonne Feb 2022

Corrigendum To “Environmental And Life-History Factors Influence Inter-Colony Multidimensional Niche Metrics Of A Breeding Arctic Marine Bird” [Sci. Total Environ. 796 (2021) 148935] (Science Of The Total Environment (2021) 796, (S0048969721040079), (10.1016/J.Scitotenv.2021.148935)), Reyd A. Smith, David J. Yurkowski, Kyle J.L. Parkinson, Jérôme Fort, Holly L. Hennin, H. Grant Gilchrist, Keith A. Hobson, Mark L. Mallory, Paco Bustamante, Jóhannis Danielsen, Svend E. Garbus, Sveinn A. Hanssen, Jón Einar Jónsson, Christopher J. Latty, Ellen Magnúsdóttir, Børge Moe, Glen J. Parsons, Christian Sonne

Great Lakes Institute for Environmental Research Publications

The authors regret that the printed version of the above article contained an omission of an individual deserving of co-authorship. The correct and final version follows. The authors would like to apologise for any inconvenience caused. < Reyd A. Smith1⁎, David J. Yurkowski2, Kyle J.L. Parkinson1, Jérôme Fort3, Holly L. Hennin4, H. Grant Gilchrist4, Keith A. Hobson5, Mark L. Mallory6, Paco Bustamante3, Jóhannis Danielsen7, Svend E. Garbus8, Sveinn A. Hanssen9, Jón Einar Jónsson10, Christopher J. Latty11, Ellen Magnúsdóttir10, Børge Moe9, Glen J. Parsons12, Christian Sonne8, Grigori Tertitski13, and Oliver P. Love1> Windsor, Windsor, Ontario, Canada, N9B 3P4 2 Fisheries and Oceans Canada, Winnipeg, Manitoba, Canada, R3T 2N6 3 Littoral, Environnement et Sociétés (LIENSs), UMR 7266 CNRS – La Rochelle University, La Rochelle, France, FR-17000 4 Environment and Climate Change Canada, Ottawa, Ontario, Canada, K0A 1H0. 5 Western University, London, Ontario, Canada, N6A 3K7 6Acadia University, Wolfville, Nova Scotia, Canada, B4P 2R6 7 Faroe Marine Research …

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Favorable Spring Conditions Can Buffer The Impact Of Winter Carryover Effects On A Key Breeding Decision In An Arctic-Breeding Seabird, Rolanda J. Steenweg, Glenn T. Crossin, Holly L. Hennin, H. Grant Gilchrist, Oliver P. Love Feb 2022

Favorable Spring Conditions Can Buffer The Impact Of Winter Carryover Effects On A Key Breeding Decision In An Arctic-Breeding Seabird, Rolanda J. Steenweg, Glenn T. Crossin, Holly L. Hennin, H. Grant Gilchrist, Oliver P. Love

Great Lakes Institute for Environmental Research Publications

The availability and investment of energy among successive life-history stages is a key feature of carryover effects. In migratory organisms, examining how both winter and spring experiences carryover to affect breeding activity is difficult due to the challenges in tracking individuals through these periods without impacting their behavior, thereby biasing results. Using common eiders Somateria mollissima, we examined whether spring conditions at an Arctic breeding colony (East Bay Island, Nunavut, Canada) can buffer the impacts of winter temperatures on body mass and breeding decisions in birds that winter at different locations (Nuuk and Disko Bay, Greenland, and Newfoundland, Canada; assessed …

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