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Haploid Plant Regeneration From Anther Cultures Of Three North American Cultivars Of Strawberry (Fragaria X Ananassa Duch.), Henry R. Owen, A. Raymond Miller
Haploid Plant Regeneration From Anther Cultures Of Three North American Cultivars Of Strawberry (Fragaria X Ananassa Duch.), Henry R. Owen, A. Raymond Miller
Henry R. Owen
A study was conducted to maxmuze plant regeneration frequencies from cultured anthers of' Chandler', 'Honeoye', and 'Redchief strawberries (Fragaria x ananassa Duch.). A comparison of auxins (IAA, NAA), cytokinins (BA, BPA, KIN) and carbohydrates (sucrose, glucose, maltose) in MS medium showed that the highest shoot regeneration across cultivars (8%) occurred when using a medium containing 2 mgtl IAA, I mgtl BA, and 0.2 M glucose. A comparison ofMS, NN, and HI inorganic medium (a new formulation based on the anther culture literature) solidified with either agar or gellan gum and containing IAA, BA, and glucose, showed the highest shoot regeneration …