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Articles 1 - 30 of 597
Full-Text Articles in Biology
Wolbachia Infection In Gall Associated Insect Communities In Illinois And Indiana, Jakeb Watts
Wolbachia Infection In Gall Associated Insect Communities In Illinois And Indiana, Jakeb Watts
Masters Theses
Wolbachia is a maternally inherited, gram-negative bacterium and has been found to be associated with up to 60% of all insects, frequently resulting in male-killing, feminization, and cytoplasmic incompatibility in the hosts. Gall wasps and other gall-forming insects induce on various host plants abnormal tissue growths, or plant galls, with distinct morphology. They are considered as obligatory parasites to their host plant. The presence of Wolbachia infection in gall wasp populations in North America has not been extensively documented. Given the strong influence on host reproduction, not knowing Wolbachia infection in insect populations prevents the understanding of how populations are …
Effects Of Wastewater Effluent On Fish Fin Morphology Of Larval Fathead Minnows (Pimephales Promelas) And Swimming Performance Of Smallmouth Buffalo (Ictiobus Bubalus), Seth Michael Bogue
Effects Of Wastewater Effluent On Fish Fin Morphology Of Larval Fathead Minnows (Pimephales Promelas) And Swimming Performance Of Smallmouth Buffalo (Ictiobus Bubalus), Seth Michael Bogue
Masters Theses
The Sangamon River flows across central Illinois and is mostly free flowing except in the city of Decatur where it is impounded to form Lake Decatur. The Sanitary District of Decatur (SDD) is located approximately three miles downstream of the Lake Decatur dam. For a significant portion of the year, the stretch of the Sangamon River downstream of SDD is dominated by effluent due to limited discharge from the Lake Decatur dam. This has resulted in heavy nutrient loading, most notably phosphorus, and elevated levels of nickel and other compounds in the downstream section of the river. Smallmouth buffalo ( …
Seasonal Variation In Mitochondrial Bioenergetics Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow Midwest River, Derick Isaac Lamptey
Seasonal Variation In Mitochondrial Bioenergetics Of The Bluegill Sunfish, Lepomis Macrochirus, From A Shallow Midwest River, Derick Isaac Lamptey
Masters Theses
As average global temperature increase, the frequency and magnitude of extreme temperatures in shallow aquatic ecosystems are more ubiquitous. In order to understand how these changing thermal regimes affect aquatic ectotherms, it is essential to develop studies evaluating the response of ectotherms to seasonal fluctuating thermal regimes. Previous studies on fluctuating temperature regimes have reported an increased physiological stress leading to morphological, behavioral and biochemical adaptations. From the latter, the adaptive capacity and seasonal performance associated with optimal function of the oxidative phosphorylation system (OXPHOS) are key for species persistence. However, studies on this matter are scarce. This study explores …
Monitoring Temporal Trends In Catostomidae Larvae In Large Sized River Tributaries, Kellie Nicole Hanser
Monitoring Temporal Trends In Catostomidae Larvae In Large Sized River Tributaries, Kellie Nicole Hanser
Masters Theses
Large river ecosystems can support a complex network of tributaries that provide optimal habitat for fish reproduction. Anthropogenic alteration of rivers and tributaries has been shown to have adverse effects on river fish communities, but few studies have focused on the effects river regulation may have on the reproduction of native suckers (Family Catostomidae). In this study, I evaluated differences in biotic and abiotic factors associated with catostomid reproduction between tributaries of an altered and free-flowing river. I conducted ichthyoplankton sampling using drift nets and larval push nets in tributaries of the Illinois River, which is regulated for navigation, and …
Dam Effects On Freshwater Mussel Growth And Recruitment In A Midwestern Stream, Taylor Lyndon Fagin
Dam Effects On Freshwater Mussel Growth And Recruitment In A Midwestern Stream, Taylor Lyndon Fagin
Masters Theses
There have been many studies that examine the negative impact of dams on freshwater mussel populations. However, recent studies suggest that some dams may create more suitable growing conditions immediately below some dams. This study’s main objectives were to 1) document where faster growth of mussels is found in relation to the Charleston Dam, 2) determine if sites with faster growth have higher recruitment and 3) investigate which water quality variable(s) could be correlated with faster growth. Live Corbicula sp. were set in cages in multiple sites at varying distances from the dam and shells of two native mussel species …
Reprograming Neuronal Cells By Overexpression Of Fibroblast-Specific Transcription Factors, Abdulmohsen Alanazi
Reprograming Neuronal Cells By Overexpression Of Fibroblast-Specific Transcription Factors, Abdulmohsen Alanazi
Masters Theses
In mammals, a complex system of regulatory signals distinguishes tissues, structures and functions. Combinations of transcription factors and co-factors regulate activation and repression of genes that result in cellular differentiation. Whole genome arrays allow the monitoring of genomic expression in specific tissues. Fibroblast microarray studies have shown candidate genes that may be involved in fibroblast identification, including genes that express transcription factors Prrx1, Snai2 and Twist1. A previous study showed that the Prrx1 and Snai2 could reactivate a fibroblast phenotype in hybrid cells that had lost fibroblast identity. Furthermore, overexpression of these factors in liver-derived cells strongly repressed liver gene …
Comparison Of The Effects Of Growing-Season Burns And Dormant-Season Burns On Vegetation Structure And Grassland Bird Diversity In East-Central Illinois Prairies, Joseph Lee Boise
Comparison Of The Effects Of Growing-Season Burns And Dormant-Season Burns On Vegetation Structure And Grassland Bird Diversity In East-Central Illinois Prairies, Joseph Lee Boise
Masters Theses
For managed grasslands, prescribed burns are a method to manage species composition by preventing crowding by bunch grasses and woody encroachment. While dormant-season (spring) burns are routinely performed, managers have recently introduced growing-season (late summer/fall) burns to prairies in east-central Illinois. The purpose of this study was to compare the effects of dormant-season and growingseason burns on vegetation structure and associated avian diversity. Specifically, objectives were to determine if: (1) Growing-season burns increase bird diversity or density of rare/declining species relative to dormant season burns; (2) Growing-season burns cause more bare ground to open and reduce litter and woody species …
Bigheaded Carp Spatial Reproductive Dynamics And Population Genetics In Large River Tributaries, Samuel J. Schaick
Bigheaded Carp Spatial Reproductive Dynamics And Population Genetics In Large River Tributaries, Samuel J. Schaick
Masters Theses
Fisheries managers are devoting considerable effort, time, and funding towards limiting the spread of invasive Bighead and Silver (bigheaded) Carp. A better understanding of factors that influence spatial patterns of bigheaded carp reproduction can help fisheries managers prevent their spread. To examine factors that influence spatial patterns of reproduction, we sampled ichthyoplankton using drift nets and larval push nets in three tributaries of the Illinois and Wabash Rivers March-September 2016-2018. We compared relative abundances of bigheaded carp eggs and larvae among tributaries using catch per unit effort (CPUE). Abiotic data were analyzed from individual study tributaries and pooled among tributaries …
Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest, Paul Switzer
Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest, Paul Switzer
Faculty Research & Creative Activity
If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.
Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest (Reduced File Size), Paul Switzer
Faculty Research & Creative Activity
If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.
Reduced File Size: Quick And Easy Guide To Common Butterflies: An Introduction To The 25 Most Commonly Encountered Butterflies In The Midwest, Paul Switzer
Faculty Research & Creative Activity
If you are new to butterfly watching, this is the book for you! It covers 25 of the most commonly encountered butterflies in the Midwestern U.S. Learning these common species will give you a great start in your discovery of the more than 100 butterfly species in our region, many of which look very similar to those included in this guide.
An Assessment Of Night Time And Seasonal Electrofishing In The Lower Wabash River, Eric Christopher Hine
An Assessment Of Night Time And Seasonal Electrofishing In The Lower Wabash River, Eric Christopher Hine
Masters Theses
Large rivers are highly important systems; being exploited both commercially and recreationally. Because of this usage by humans, close monitoring of the ecology of theses rivers is of the utmost importance. The Long-Term Electrofishing project (LTEF) monitors the fish communities of the Illinois, Mississippi, Wabash, and Ohio rivers using day time, pulsed-DC electrofishing during the late Spring through the early Fall each year. Given that previous studies have noted diel and seasonal changes in catch and composition of fish communities, the addition of night time electrofishing may be beneficial to the overarching goals of the LTEF. This study sought to …
The Role Of Red Leaf Coloration In Prey Capture For Pinguicula Planifolia, Jenna Annis, Janice Coons, Charles Helm, Brenda Molano-Flores
The Role Of Red Leaf Coloration In Prey Capture For Pinguicula Planifolia, Jenna Annis, Janice Coons, Charles Helm, Brenda Molano-Flores
Faculty Research & Creative Activity
Anthocyanins in the leaves of carnivorous plants are suggested to play a role inprey capture. In this study, we investigated the role of red leaf coloration (an indicator of anthocyanins)on prey capture using Pinguicula planifolia (Chapman’s Butterwort). Overall,red leaves had less prey (i.e., Collembola) than green leaves, suggesting that red colorationdoes not enhance prey capture for Chapman’s Butterwort. However, the frequent presenceof Collembola on leaves suggests that this plant species could be relying on other cues toattract prey (e.g., olfactory cues).
A New Species Of Cyperus Section Incurvi (Cyperaceae) From Venezuela, Gordon Tucker
A New Species Of Cyperus Section Incurvi (Cyperaceae) From Venezuela, Gordon Tucker
Faculty Research & Creative Activity
Cyperus fedoniae is described as a new species. It is known from a single collection from the lowlands of southern Venezuela. It is a member of section Incurvi. It is related to C. dichromeniformis and C. inops, from which it can be distinguished by its smaller size and features of spikelets and achenes. A distinctive feature is the arching culms which take root at the base of the spikelets.Cyperus fedoniae es descrito como una nueva especie. Se conoce de una sola muestra de las tierras bajas del sur de Venezuela. Es un miembro de la secci.n Incurvi. Est. relacionada con …
Tradeoffs Of Warm Adaptation In Aquatic Ectotherms: Live Fast, Die Young?, A. P. Porreca, E. Martinez, R. E. Colombo, Michael A. Menze
Tradeoffs Of Warm Adaptation In Aquatic Ectotherms: Live Fast, Die Young?, A. P. Porreca, E. Martinez, R. E. Colombo, Michael A. Menze
Faculty Research & Creative Activity
In the face of a changing climate, questions regarding sub-lethal effects of elevated habitat temperature on the physiology of ectotherms remain unanswered. In particular, long-term responses of ectotherms to the warming trend in tropical regions are unknown and significantly understudied due primarily to the difficulties in specimen and community traceability. In freshwater lakes employed as cooling reservoirs for power plants, increased physiological stress from high water temperature can lead to an increase in mortality, reduce growth, and potentially alter the community structure of fishes. Throughout this study, we employ this highly tractable system to assess how elevated thermal regimes can …
Streamwise Vortices Destabilize Swimming Bluegill Sunfish (Lepomis Macrochirus), Anabela Maia, Alex P. Sheltzer, Eric D. Tytell
Streamwise Vortices Destabilize Swimming Bluegill Sunfish (Lepomis Macrochirus), Anabela Maia, Alex P. Sheltzer, Eric D. Tytell
Faculty Research & Creative Activity
In their natural environment, fish must swim stably through unsteady flows and vortices, including vertical vortices, typically shed by posts in a flow, horizontal cross-flow vortices, often produced by a step or a waterfall in a stream, and streamwise vortices, where the axis of rotation is aligned with the direction of the flow. Streamwise vortices are commonly shed by bluff bodies in streams and by ships' propellers and axial turbines, but we know little about their effects on fish. Here, we describe how bluegill sunfish use more energy and are destabilized more often in flow with strong streamwise vorticity. The …
Geographic Range Predicts Photosynthetic And Growth Response To Warming In Co-Occurring Tree Species, Peter B. Reich, Kerrie M. Sendall, Karen Rice, Roy L. Rich, Artur Stefanski, Sarah E. Hobbie, Rebecca A. Montgomery
Geographic Range Predicts Photosynthetic And Growth Response To Warming In Co-Occurring Tree Species, Peter B. Reich, Kerrie M. Sendall, Karen Rice, Roy L. Rich, Artur Stefanski, Sarah E. Hobbie, Rebecca A. Montgomery
Faculty Research & Creative Activity
No abstract provided.
Molecular Approaches For Improving Desiccation Tolerance: Insights From The Brine Shrimp Artemia Franciscana, Steven C. Hand, Michael A. Menze
Molecular Approaches For Improving Desiccation Tolerance: Insights From The Brine Shrimp Artemia Franciscana, Steven C. Hand, Michael A. Menze
Faculty Research & Creative Activity
Main conclusion We have evaluated the endogenous expression and molecular properties of selected Group 3 LEA proteins from Artemia franciscana , and the capacity of selected Groups 1 and 3 proteins transfected into various desiccation-sensitive cell lines to improve tolerance to drying. Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons—exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages …
Tradeoffs Of Warm Adaptation In Aquatic Ectotherms: Live Fast, Die Young?, Eloy Martinez, Anthony Porreca, Robert Colombo, Michael Menze
Tradeoffs Of Warm Adaptation In Aquatic Ectotherms: Live Fast, Die Young?, Eloy Martinez, Anthony Porreca, Robert Colombo, Michael Menze
Faculty Research & Creative Activity
In the face of a changing climate, questions regarding sub-lethal effects of elevated habitat temperature on the physiology of ectotherms remain unanswered. In particular, long-term responses of ectotherms to the warming trend in tropical regions are unknown, and understudied due to the difficulties in specimen and community traceability. In freshwater lakes employed as cooling reservoirs for power plants, increased physiological stress from high water temperature can potentially alter the community structure of fishes. We employ this highly tractable system to assess how thermal regimes can alter the physiology and ecology of aquatic species. We documented a significantly reduced lifespan, growth …
Acclimation Of Photosynthetic Temperature Optima Of Temperate And Boreal Tree Species In Response To Experimental Forest Warming, Kerrie M. Sendall, Peter B. Reich, Changming Zhao, Hou Jihua, Xia Orong Wei, Artur Stefanski, Karen Rice, Roy L. Rich, Rebecca A. Montgomery
Acclimation Of Photosynthetic Temperature Optima Of Temperate And Boreal Tree Species In Response To Experimental Forest Warming, Kerrie M. Sendall, Peter B. Reich, Changming Zhao, Hou Jihua, Xia Orong Wei, Artur Stefanski, Karen Rice, Roy L. Rich, Rebecca A. Montgomery
Faculty Research & Creative Activity
Rising temperatures caused by climate change could negatively alter plant ecosystems if temperatures exceed optimal
temperatures for carbon gain. Such changes may threaten temperature-sensitive species, causing local extinctions and
range migrations. This study examined the optimal temperature of net photosynthesis (Topt) of two boreal and four
temperate deciduous tree species grown in the field in northern Minnesota, United States under two contrasting temperature
regimes. We hypothesized that Topt would be higher in temperate than co-occurring boreal species, with
temperate species exhibiting greater plasticity in Topt, resulting in better acclimation to elevated temperatures. The
chamberless experiment, located at two sites in …
Group 3 Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana: Structural Properties And Protective Abilities During Desiccation, Leaf C. Boswell, Michael A. Menze, Steven C. Hand
Group 3 Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana: Structural Properties And Protective Abilities During Desiccation, Leaf C. Boswell, Michael A. Menze, Steven C. Hand
Faculty Research & Creative Activity
Group 3 late embryogenesis abundant (LEA) proteins are highly
hydrophilic, and their expression is associated with desiccation
tolerance in both plants and animals. Here we show that two
LEA proteins from embryos of Artemia franciscana, AfrLEA2
and AfrLEA3m, are intrinsically disordered in solution but
upon desiccation gain secondary structure, as measured by circular
dichroism. Trifluoroethanol and sodium dodecyl sulfate
are both shown to induce a-helical structure in AfrLEA2 and
AfrLEA3m. Bioinformatic predictions of secondary-structure
content for both proteins correspond most closely to conformations
measured in the dry state. Because some LEA proteins
afford protection to desiccation-sensitive proteins during drying
and …
Cryopreservation Of Hepatocyte (Hepg2) Cell Monolayers: Impact Of Trehalose, Blake Stokich, Quinn Osgood, David Grimm, Shhyam Moorthy, Nilay Chakraborty, Michael A. Menze
Cryopreservation Of Hepatocyte (Hepg2) Cell Monolayers: Impact Of Trehalose, Blake Stokich, Quinn Osgood, David Grimm, Shhyam Moorthy, Nilay Chakraborty, Michael A. Menze
Faculty Research & Creative Activity
A simple method to cryogenically preserve hepatocyte monolayers is currently not available but such a technique would facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. We investigated the effect of trehalose and dimethyl sulfoxide (Me2SO) in cryopreservation of human hepatocellular carcinoma (HepG2) cells in suspension and monolayer formats. HepG2 cell monolayers were incubated for 24 h at varying concentrations of trehalose (50–150 mM) prior to cryopreservation to identify the optimum concentration for such preincubation. When trehalose alone was used as the cryoprotective agent (CPA), cells in monolayer format did not survive freezing while …
Are U, Ni, And Hg An Environmental Risk Within A Rcra/Cercla Unit On The U.S. Department Of Energy's Savannah River Site?, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas
Are U, Ni, And Hg An Environmental Risk Within A Rcra/Cercla Unit On The U.S. Department Of Energy's Savannah River Site?, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas
Faculty Research & Creative Activity
The U.S. Department of Energy's Savannah River Site (SRS) is a former nuclear weapon production facility. From 1954–1985, releases of Al, Cu, Cr, Hg, Ni, Pb, U, and Zn were discharged into the Tims Branch-Steed Pond water system. This study investigates whether metal concentrations in Tims Branch's sediment, biofilm and other biota exceed screening level risk calculations to determine if remedial actions should be pursued for the Contaminants of Potential Concern (U, Ni, Hg). Transfer factors (TFs) were calculated to determine metal concentration changes throughout lower trophic levels and results were compared with sediment benchmarks to create hazard quotients (HQs) …
Trophic Dynamics Of U, Ni, Hg And Other Contaminants Of Potential Concern On The Department Of Energy’S Savannah River Site, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas
Trophic Dynamics Of U, Ni, Hg And Other Contaminants Of Potential Concern On The Department Of Energy’S Savannah River Site, Paul G. Edwards, Karen F. Gaines, A. Lawrence Bryan Jr., James M. Novak, Susan A. Blas
Faculty Research & Creative Activity
The Department of Energy’s Savannah River Site is a former nuclear weapon material production and current research facility located in South Carolina, USA. Wastewater discharges from a fuel and nuclear reactor target manufacturing facility released depleted and natural U, as well as other metals into the Tims Branch- Steed Pond water system. We investigated the current dynamics of this system for the purposes of environmental monitoring and assessment by examining metal concentrations, bioavailability, and trophic transfer of contaminants in seven ponds. Biofilm, detritus, and Anuran and Anisopteran larvae were collected and analyzed for stable isotopes (δ15N, δ13C) and contaminants of …
Genetic Engineering, A Hope For Sustainable Biofuel Production: Review, Sudip Paudel, Michael A. Menze
Genetic Engineering, A Hope For Sustainable Biofuel Production: Review, Sudip Paudel, Michael A. Menze
Faculty Research & Creative Activity
The use of recently developed genetic engineering tools in combination with organisms that have the potential to produce precursors for the production of biodiesel, promises a sustainable and environment friendly energy source. Enhanced lipid production in wild type and/or genetically engineered organisms can offer sufficient raw material for industrial transesterification of plant-based triglycerides. Bio-diesel, produced with the help of genetically modified organisms, might be one of the best alternatives to fossil fuels and to mitigate various environmental hazards.
Toxicodynamic Modeling Of 137cs To Estimate White-Tailed Deer Background Levels For The Department Of Energy's Savannah River Site, Karen F. Gaines, James M. Novak, Christopher W. Bobryk, Susan A. Blas
Toxicodynamic Modeling Of 137cs To Estimate White-Tailed Deer Background Levels For The Department Of Energy's Savannah River Site, Karen F. Gaines, James M. Novak, Christopher W. Bobryk, Susan A. Blas
Faculty Research & Creative Activity
The U.S. Department of Energy's (USDOE) Savannah River Site (SRS) is a former nuclear weapon material production and current research facility adjacent to the Savannah River in South Carolina, USA. The purpose of this study was to determine the background radiocesium (137Cs) body burden (e.g., from global fallout) for white-tailed deer (Odocoileus virginianus) inhabiting the SRS. To differentiate what the background burden is for the SRS versus 137Cs obtained from SRS nuclear activities, data were analyzed spatially, temporally and compared to other off-site hunting areas near the SRS. The specific objectives of this study were: to compare SRS and offsite …
Identification Of Disufide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle
Identification Of Disufide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle
Faculty Research & Creative Activity
MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. Proteomic analysis identified the specific cysteine residues that participate in the disulfide bond. This is the first report that effectively links mitoNEET to activation of the insulin regulator GDH1.
Differential Habitat Use By Common Watersnakes (Nerodia Sipedon), Lorin A. Neuman-Lee, Andrew M. Durso, Nicholas M. Kiriazis, Melanie J. Olds, Stephen J. Mullin
Differential Habitat Use By Common Watersnakes (Nerodia Sipedon), Lorin A. Neuman-Lee, Andrew M. Durso, Nicholas M. Kiriazis, Melanie J. Olds, Stephen J. Mullin
Faculty Research & Creative Activity
Understanding intraspecific variation in habitat use is important for the management of any species. In many studies of reptiles, habitat use by juveniles is poorly understood when compared to their adult conspecifics because of capture biases and logistical constraints. We compared habitat use between sexes and age classes of Common Watersnakes (Nerodia sipedon) at a reservoir in central Illinois. Juvenile N. sipedon occurred more frequently in habitat with high canopy cover, whereas adult N. sipedon, especially reproductive females, were found exclusively in habitat with no canopy cover. Adult males used both locations equally. We emphasize the need to investigate ontogenetic …
Identification Of Disulfide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle
Identification Of Disulfide Bond Formation Between Mitoneet And Glutamate Dehydrogenase 1, Morgan E. Roberts, Jacquelyn P. Crail, Megan M. Laffoon, William G. Fernandez, Michael A. Menze, Mary E. Konkle
Faculty Research & Creative Activity
MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding partner. GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. Proteomic analysis identified the specific cysteine residues that participate in the disulfide bond. This is the first report that effectively links mitoNEET to activation of the insulin regulator GDH1.
Mitochondrial Energetics Of Benthic And Pelagic Antarctic Teleosts, Eloy Martinez, Michael A. Menze, Joseph P. Torres
Mitochondrial Energetics Of Benthic And Pelagic Antarctic Teleosts, Eloy Martinez, Michael A. Menze, Joseph P. Torres
Faculty Research & Creative Activity
Antarctic fauna are highly adapted to the frigid waters of the Southern Ocean. This study describes the in vitro temperature sensitivity of oxygen consumption rates measured in liver mitochondria from the pelagic notothenioid Pleuragramma antarcticum between 5 and 35 C. Oxygen fluxes were measured after the addition of millimolar levels of pyruvate, malate, succinate and glutamate (state II, LEAK) and saturating levels of ADP [state III, oxidative phosphorylation (OXPHOS)]. State III respiration significantly decreased above 18.7 C. A comparison of the oxidative capacities among P. antarcticum and other notothenioids showed significant differences in state III respiration, where benthic species exhibited …