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Articles 1 - 6 of 6

Full-Text Articles in Molecular Biology

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee Jan 1999

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee

Retrospective Theses and Dissertations

We have examined the developmental distribution and differential localization of presynaptic terminal associated proteins in the mammalian retina. We have used antibodies specific for synaptic vesicle associated proteins Synaptotagmin, Rab 3A, Synaptophysin and Synaptobrevin, and presynaptic terminal membrane associated proteins SNAP-25 and Syntaxin, to characterize their spatio-temporal distribution during retinal differentiation and in the mature retina;The vertebrate retina has a laminar organization consisting of three cellular layers separated by two synaptic layers, the inner piexiform layer and the outer plexiform layer. In general, immunoreactivity for presynaptic terminal associated proteins was first observed in cellular layers, and as differentiation progressed ...


Transposon-Induced Homologous Recombination At The Maize P Locus And In Transgenic Arabidopsis , Yongli Xiao Jan 1999

Transposon-Induced Homologous Recombination At The Maize P Locus And In Transgenic Arabidopsis , Yongli Xiao

Retrospective Theses and Dissertations

The maize P gene encodes a Myb-homologous regulator of red phlobaphene pigment biosynthesis in the pericarp, cob and other floral tissues. The P locus has a unique structure with two 5.2kb direct repeats flanking the P gene coding region. When the transposon (Ac) inserts into one site between the two direct repeats in the P-ovov-1114 allele, homologous recombination between the two 5.2kb repeats can occur and the whole P gene coding sequence is deleted;To further study this transposon-induced homologous recombination. This study examined six alleles that carry Ac insertions at different sites in the P locus, in ...


Genome Reorganization And Non-Linear Transposition In Maize , Jianbo Zhang Jan 1999

Genome Reorganization And Non-Linear Transposition In Maize , Jianbo Zhang

Retrospective Theses and Dissertations

Transposable elements have long been considered as potential agents of large-scale genome reorganization by virtue of their ability to induce chromosomal rearrangements such as deletions, duplications, inversions, and reciprocal translocations. Previous researchers have shown that particular configurations of transposon termini can induce chromosome rearrangements at high frequencies. By analyzing two derivatives of an unstable allele of the maize P1 (pericarp color) gene carrying both a full-length Ac (Activator) transposable element and a Ac terminal fragment termed fAc (fractured Ac) that were recovered from a classical maize ear twinned sector, it were found that the twinned alleles are a large inverted ...


Molecular And Genetic Analysis Of Jil-1, A Chromatin Associated Protein Kinase Implicated In Transcriptional Regulation In Drosophila , Ye Jin Jan 1999

Molecular And Genetic Analysis Of Jil-1, A Chromatin Associated Protein Kinase Implicated In Transcriptional Regulation In Drosophila , Ye Jin

Retrospective Theses and Dissertations

Chromatin remodeling is an essential step for many chromatin-related fundamental cellular processes, such as gene replication, transcription, recombination and gene silencing. Two classes of protein complexes contribute to chromatin remodeling: protein complexes with ATPase activity and protein complexes capable of enzymatic modifications of chromosomal proteins, especially histones. Compared to well-studied histone acetylation which has been directly linked to gene transcription, the role of histone phosphorylation in chromatin remodeling for gene transcription is still poorly understood although there is some correlation between histone phosphorylation and gene transcription;A novel nuclear tandem protein kinase JIL-1 was cloned and identified in Kristen Johansen ...


Molecular Characterization Of Myb-Homologous Transcriptional Factors Of The Flavonoid Pathway In Zea Mays , Peifen Zhang Jan 1999

Molecular Characterization Of Myb-Homologous Transcriptional Factors Of The Flavonoid Pathway In Zea Mays , Peifen Zhang

Retrospective Theses and Dissertations

Structural genes encoding enzymes that catalyze each step of flavonoid synthesis are temporally and spatially regulated. It has been shown that specific anthocyanin pigmentation patterns in maize are achieved by the tissue-specific expression of regulatory genes. Moreover, this tissue-specific regulation is a consequence of the generation of different alleles or duplicated copies of regulatory genes during evolution;In contrast, the myb-homologous P1 gene was the only known regulatory gene required for phlobaphene biosynthesis. The isolation and characterization of the P2 gene, reported here, provides direct molecular evidence that the P region contains duplicated genes. Furthermore, the duplicated P1 (herein, P1-rr ...


Novel G-Dna Binding Proteins From The Ciliate Tetrahymena Thermophila: Purification, Characterization, Cloning And Functional Analyses , Quan Lu Jan 1999

Novel G-Dna Binding Proteins From The Ciliate Tetrahymena Thermophila: Purification, Characterization, Cloning And Functional Analyses , Quan Lu

Retrospective Theses and Dissertations

G-DNA is a family of novel four-stranded DNA structures characterized by motifs called G-quartets. Evidence is growing to suggest that G-DNA exists and plays biological roles in vivo. In order to further elucidate the functions of G-DNA, we have studied proteins that specifically bind to the DNA structure;Two G-DNA binding activities, TGPI and TGP3, were purified from the ciliate Tetrahymena thermophila. Based on the peptide sequences obtained from direct internal peptide sequencing, the cDNAs coding for the genes were cloned. Deduced protein sequences showed that TGP1 and TGP3 are novel proteins but share significant homology with each other. Furthermore ...