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Full-Text Articles in Molecular Biology
Cloning, Sequencing, And Expression Of Brucella Abortus Heat Shock 70 Gene , Emily C. L. Chin
Cloning, Sequencing, And Expression Of Brucella Abortus Heat Shock 70 Gene , Emily C. L. Chin
Retrospective Theses and Dissertations
Brucella abortus is a gram negative bacteria that can infect and cause serious disease in many mammals including humans. Any organism, including Brucella, that can survive as an intracellular parasite must be able to survive the stress of a foreign and often hostile environment of the host. Stress usually results in a dramatic change in gene expression including an elevated synthesis of heat shock proteins;We have subcloned, sequenced and expressed the Brucella HSP70 and the upstream sequences. The gene was put under the control of the strong "tac" promoter. However, when expressed, we saw a high accumulation of a ...
Molecular Analysis Of Protein Kinase C In The Sea Urchin Lytechinus Pictus , Terese Louise Rakow
Molecular Analysis Of Protein Kinase C In The Sea Urchin Lytechinus Pictus , Terese Louise Rakow
Retrospective Theses and Dissertations
Protein kinase C (PKC) was originally described as a serine threonine kinase in brain tissue. This activity was found to be dependent upon Ca[superscript]2+ and phosphatidyl serine. Today protein kinase C consists of a family of kinases with nine known members. Some members are Ca[superscript]2+-independent isozymes, and all of them have subtle activation differences. The multiple isozymes with varying activities in addition to different tissue distributions lead to the idea that members of the family have different functions. PKC has been well studied in mammalian systems and it is concluded that PKC is activated in ...
Cloning And Molecular Characterization Of Two Nuclear Genes For Zea Mays Mitochondrial Chaperonin 60 , Pamela Sue Close
Cloning And Molecular Characterization Of Two Nuclear Genes For Zea Mays Mitochondrial Chaperonin 60 , Pamela Sue Close
Retrospective Theses and Dissertations
Hsp60 is a mitochondrial protein first identified in Tetrahymena thermophila and Saccharomyces cerevisiae. It belongs to a class of proteins called "molecular chaperones" which prevent aggregation and precipitation of partially or completely unfolded polypeptides;Two cDNAs for maize cpn60 were identified and sequenced. Southern blots of maize DNA showed two bands hybridizing with mt-cpn60 in maize inbreds. One copy was present in Arabidopsis. Both maize genes are contained within the nuclear genome. The chromosomal location of the cpn60 genes have been mapped by RFLPs. A maize B73 genomic library has been constructed and screened for cpn60 clones and two major ...
Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu
Characterization Of Cis-Acting Replication Control Elements In Ribosomal Rna Genes Of Tetrahymena Thermophila , Wen-Ling Shaiu
Retrospective Theses and Dissertations
This dissertation describes the identification and characterization of specific cis-acting replication control elements in the replication origin of Tetrahymena rDNA. Earlier studies of a mutant rDNA allele, C3-rmm1, identified a cis-acting sequence (the Type I repeat) within the 5[superscript]' non-transcribed spacer of rDNA that affects rDNA replication (Larson et al. 1986). Characterization of two additional, independently-isolated mutants (C3-rmm3 and C3-rmm4) and a revertant of C3-rmm4 strongly argue that the Type I repeat is indeed a cis-acting replication control element. Mutations causing rDNA replication defects were identified in copies of the Type I repeat that are separated by >600 bp ...
Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson
Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson
Retrospective Theses and Dissertations
Prior to licensing of veterinary vaccines, it is essential to demonstrate that the use of the product is safe (e.g., use of the product will not have unexpected or undesirable effects on animals exposed to the agent or on the environment) and potent (e.g., produces the desired result). The projects described in this dissertation demonstrate research directed toward the development and improvement of safety and potency testing of veterinary vaccines from a regulatory perspective;In the first study, recombination between vaccine strains of pseudorabies virus (PRV) resulting in the creation of strains with undesirable virulence and antigenic combinations ...
Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun
Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun
Retrospective Theses and Dissertations
Proteins that bind to specific sequences in replication origins have been shown to be important components of both prokaryotic and eukaryotic DNA replication systems. An origin of DNA replication is located within the 5[superscript]' non-transcribed spacer region of the rRNA genes (rDNA) of Tetrahymena thermophila. Previous work has shown that several cis-acting mutations responsible for rDNA replication defects lie in upstream repeats of a transcriptional promoter element, the Type I repeat;Using FE(II)EDTA cleavage footprinting and gel-mobility shift assays, I identified and characterized two distinct DNA-binding activities that interact with the Type I repeat. One of these ...