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Zoology and genetics

Medicine and Health Sciences

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Full-Text Articles in Molecular Biology

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee Jan 1999

Dynamic Changes In The Localization Of Synapse Associated Proteins During Development And Differentiation Of The Mammalian Retina , Mary Heather West Greenlee

Retrospective Theses and Dissertations

We have examined the developmental distribution and differential localization of presynaptic terminal associated proteins in the mammalian retina. We have used antibodies specific for synaptic vesicle associated proteins Synaptotagmin, Rab 3A, Synaptophysin and Synaptobrevin, and presynaptic terminal membrane associated proteins SNAP-25 and Syntaxin, to characterize their spatio-temporal distribution during retinal differentiation and in the mature retina;The vertebrate retina has a laminar organization consisting of three cellular layers separated by two synaptic layers, the inner piexiform layer and the outer plexiform layer. In general, immunoreactivity for presynaptic terminal associated proteins was first observed in cellular layers, and as differentiation progressed ...


Regulation Of Brucella Abortus Catalase As A Defensive Mechanism Against Oxidative Stress , Jeonga Kim Jan 1997

Regulation Of Brucella Abortus Catalase As A Defensive Mechanism Against Oxidative Stress , Jeonga Kim

Retrospective Theses and Dissertations

Free radicals in the nature are harmful to organisms because they cause damage to tissues, cell membranes, proteins, and DNA. The animal immune system (cell mediated immune response) uses oxygen molecules to kill foreign organisms. B. abortus causes a cattle disease called brucellosis. The mechanism for bacteria to survive and adapt in the face of oxidative attack is thought to be important for understanding pathogenesis. This dissertation presents the response of B. abortus to oxidative stress and the regulation of catalase as a defensive mechanism;Different protein expression patterns were observed during oxidative stress by conducting 2-D protein gel analysis ...


Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha Jan 1994

Purification, Characterization And Cloning Of A Periplasmic Catalase From B Abortus And The Role It Plays In The Pathogenesis Of Brucella , Zheng Yu Sha

Retrospective Theses and Dissertations

A periplasmic catalase has been identified and purified from Brucella abortus using a procedure which fractionates periplasmic B. abortus proteins from other cellular components while retaining biological activity. The biochemical characteristics of the purified enzyme were studied and the gene encoding the enzyme cloned and sequenced. SDS polyacrylamide gel electrophoresis of the purified enzyme revealed only a single subunit with a molecular weight of 59,000. Gel filtration indicates the native protein is a tetramer. The native protein has a visible absorption spectrum with maxima at 407, 501, and 628 nm which is typical of mammalian, but not some bacterial ...


Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor Jan 1994

Characterization And Immunogenicity Of The Heat Shock Protein, Hsp60 Of Brucella Abortus , Dennis Gor

Retrospective Theses and Dissertations

Brucella abortus is the causative agent of bovine brucellosis, a disease that causes spontaneous abortion in cows. The most effective form of protection against this pathogen is immunization; however, Brucella abortus strain 19 (the standard vaccine against Brucella abortus) is not completely protective. The vaccination of laboratory mice with the heat shock protein, hsp60 derived from Brucella abortus, using live attenuated recombinant Salmonella typhimurium expressing this protein (hsp60) as a delivery system, and the characterization of the immune response to Brucella abortus hsp60 over a 12 week time course following vaccination is described;Infection of BALB/c strain of laboratory ...


Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson Jan 1993

Safety And Potency Testing Of Veterinary Vaccines , Louise Mary Henderson

Retrospective Theses and Dissertations

Prior to licensing of veterinary vaccines, it is essential to demonstrate that the use of the product is safe (e.g., use of the product will not have unexpected or undesirable effects on animals exposed to the agent or on the environment) and potent (e.g., produces the desired result). The projects described in this dissertation demonstrate research directed toward the development and improvement of safety and potency testing of veterinary vaccines from a regulatory perspective;In the first study, recombination between vaccine strains of pseudorabies virus (PRV) resulting in the creation of strains with undesirable virulence and antigenic combinations ...