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Molecular Biology Commons

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Zoology and genetics

Genetics

1997

Articles 1 - 3 of 3

Full-Text Articles in Molecular Biology

Host Regulation Of The Replication Of The Yeast Retrotransposon Ty5 , Ning Ke Jan 1997

Host Regulation Of The Replication Of The Yeast Retrotransposon Ty5 , Ning Ke

Retrospective Theses and Dissertations

The replication of retroelements may have deleterious effects on the hosts. Therefore, elements and their hosts have coevolved mechanisms to regulate retroelement replication. This regulation can occur at the transcriptional, translational, or post-translational levels as well as through targeted integration or cDNA recombination. I have studied host regulation of the Saccharomyces retrotransposon Ty5. I have found that Ty5 transcription is carefully controlled. Transcription of Ty5 is haploid specific and is repressed 10 fold in diploid strains. Ty5 transcription is also regulated by the pheromone response pathway, which is activated during mating. In addition, Ty5 transcription is regulated by flanking sequences ...


Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen Jan 1997

Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen

Retrospective Theses and Dissertations

Aspergillus awamori glucoamylase (GA) is an enzyme involved in industrial corn starch processing. Genetic and biochemical approaches were used to study the mechanisms governing GA thermostability. Three proline substitution (Xaa → Pro) mutations were constructed that were predicted to increase the enzyme's stability by decreasing its conformational entropy of unfolding. When expressed in Saccharomyces cerevisiae Ser30 → Pro increased, Asp345 → Pro did not alter and Glu408 → Pro greatly decreased GA stability as measured by resistance to irreversible thermoinactivation relative to the wild-type enzyme. The Ser30 → Pro mutation was combined with other previously identified stabilizing mutations to examine whether combining such mutations ...


Cloning And Characterization Of A Soybean Snf2 Yeast Homolog , Weiping Chen Jan 1997

Cloning And Characterization Of A Soybean Snf2 Yeast Homolog , Weiping Chen

Retrospective Theses and Dissertations

It is of great economic and scientific interest to understand plant disease resistance genes at the molecular level. Phytophthora sojae, a fungal pathogen of soybean is of particular interest since the root and stem rot resulting from its infection causes great economic loss. It is the goal of this project to clone Phytophthora resistance genes from soybean. This was done by designing primers from the conserved regions of known plant disease resistance genes using a modified differential display for near isogenic lines; L85-3044 a resistant line and Williams, a susceptible line. Several polymorphic bands were identified, including a unique 0 ...