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Molecular Biology Commons

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Zoology and genetics

Biochemistry

1993

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Full-Text Articles in Molecular Biology

Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun Jan 1993

Characterization Of Dna-Binding Proteins That Recognize The Replication Origin Of The Rrna Genes Of Tetrahymena Thermophila , Angela Rae Umthun

Retrospective Theses and Dissertations

Proteins that bind to specific sequences in replication origins have been shown to be important components of both prokaryotic and eukaryotic DNA replication systems. An origin of DNA replication is located within the 5[superscript]' non-transcribed spacer region of the rRNA genes (rDNA) of Tetrahymena thermophila. Previous work has shown that several cis-acting mutations responsible for rDNA replication defects lie in upstream repeats of a transcriptional promoter element, the Type I repeat;Using FE(II)EDTA cleavage footprinting and gel-mobility shift assays, I identified and characterized two distinct DNA-binding activities that interact with the Type I repeat. One of these ...